马铃薯野生种试管微型薯诱导研究     

张易  杨清  陈敏 《园艺学报》2006,33(2):369-369

马铃薯野生种是重要遗传资源。微型薯诱导技术作为重要繁殖方法在栽培种上已得到广泛应用,但是在野生种上迄今仍鲜见报道。本研究以3个马铃薯野生种Solanumpinnatisectum,Solanumcardiophyllum和Solanumbulbocastanum为材料进行微型薯诱导试验。将带有两个节间的茎段外植体接种  相似文献   

Implantation study on the potential of murine epidermal stem cell differentiation     

LIN Yi  LI Hai-biao  HUANG Jin-tao  ZENG Shan  DI Jing-fang  ZENG Yao-ying 《园艺学报》2006,22(12):2418-2423

AIM: To investigate the potential of murine epidermal stem cell (ESC) differentiation after seeded in a biodegradable carrier and implanted subcutaneously into syngeneic recipient mice. METHODS: ES cells were induced in vitro to differentiate into ESCs. After stained with a fluorescent dye Hoechst 33342, these ESCs were seeded into a polyglycolic acid (PGA) net containing collagen gel, functioning as a cell carrier, and implanted subcutaneously into 129/J mice, which were syngeneic to these stem cells. RESULTS: The ESCs kept alive in the implant when observed under a fluorescent microscopy 3 weeks or longer after implantation, and could differentiate into hair follicle-like structure, glandular structure, and gave rise to additional structures displaying features resembling native dermis. No apparent rejection or severe side effects were observed at least 10 weeks post-implantation. CONCLUSION: It is feasible to use these ESCs as seed cells in the study to fabricate dermal equivalent having the potential to develop dermal appendages.  相似文献   

Effect of reactive oxygen species on the growth and apoptosis-related gene expression in ECV304 cells     

XIE Li-xia  YAO Shu-ming  ZHANG Xuan  HU Yi  CHEN Peng  ZHANG Heng-li  LI Li  WANG Dian-hua 《园艺学报》2006,22(10):2055-2058

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Detection and analyses of coagulation and fibrolysis factor mRNA levels in tissue of hepatocellular carcinoma patients     

ZHOU Qi  LI Shao-qiang  LIANG Li-jian  PENG Bao-gang  ZHEN Yu-yang  SU Cai-kun 《园艺学报》2006,22(7):1348-1352

AIM:To detect the mRNA expression of tissue factor (TF), urokinase-type plasminogen activator (u-PA) and urokinase-type plasminogen activator receptor (u-PAR) in the samples of hepatocellular carcinoma (HCC) and adjacent tissue, and to elucidate their association with clinical significance. METHODS:The mRNA levels of TF, u-PA and u-PAR in 27 human HCC tissue samples with their adjacent tissue samples and 27 normal liver tissues were detected by RT-PCR. The relationship between the mRNA expression and their clinic-pathological data were also analyzed. RESULTS:The expressive rate of TF, uPA and uPAR in HCC tissue samples was 62.96% (17/27), 70.37% (19/27), 77.78% (21/27), respectively and relative expression intensity of TF, uPA and uPAR were 0.567±0.268, 0.964±0.458 and 0.784±0.322, respectively, which were significantly higher than those in adjacent tissue group and normal liver group (P<0.05). TF, uPA and uPAR mRNA levels were all significantly higher in larger size and in the invasive and metastatic subgroup (P<0.05). There had positive relationship between the mRNA expressions of these three factors. CONCLUSION:The results indicate that TF, uPA and uPAR might play an important role in hepatic carcinogenesis, invasiveness and metastasis.  相似文献   

马铃薯卷叶病毒云南分离物外壳蛋白基因的克隆与序列分析     

丁铭  方琦  李婷婷  张丽珍  董家红  李展  张仲凯 《植物病理学报》2006,36(5):473-476

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条斑型玉米圆斑病病原鉴定及其生物学特性研究   总被引：5，自引：0，他引：5  

孙广宇  王琴  张荣  毛琦 《植物病理学报》2006,36(6):494-500

 本研究通过致病性测定、形态学和分子生物学鉴定方法,首次明确陕西省发生的玉米条斑型叶斑病是玉米圆斑病的一种症状类型,由玉米生离蠕孢(Bipolaris zeicola)3号生理小种引起。供试品种豫玉22、农大108、农大3138、东单7号、沈单10号、沈单16号、户单4号、新陕资1号、高农1号和陕单9号都对3号生理小种表现感病,品种间抗病性差异不显著。此菌在PDA、PSA、OMA、CMA、WA和Czapek上均可生长,最适生长培养基为PDA。菌落生长温度为5~35℃,最适菌落生长温度为25℃。分生孢子在5~40℃均可萌发,低于5℃高于40℃萌发率极低,萌发的最适温度为28℃。孢子在pH 3~11均可萌发,萌发最适pH为5。在自来水中分生孢子可以萌发,蔗糖和葡萄糖溶液可以明显促进孢子萌发。  相似文献   

烟草线条病毒RT-PCR检测方法   总被引：1，自引：0，他引：1  

魏梅生  相宁  张春泉  Said A.Ghabrial 《植物检疫》2006,20(3):136-138

根据已报道的烟草线条病毒外壳蛋白基因序列，设计出一对特异性引物，提取大豆病叶的总RNA，反转录成cDNA，PCR扩增出约400bp大小的产物，产物克隆到pGEM-T Easy载体上，转化大肠杆菌DH 5α，抽提的质粒用Not Ⅰ酶切，出现430bp大小的目标插入片段。经序列测定确认了插入片段的大小为404bp。  相似文献   

苹果轮纹病药剂筛选与药剂配方   总被引：6，自引：0，他引：6  

高艳敏  李广旭  沈永波  王毅  王佳军  王斌  马怀尧 《果树学报》2006,23(3):401-405

为解决苹果枝干轮纹病有效防治药剂缺乏的问题,1998—1999年在富士苹果枝干轮纹病树上开展其病害的药剂配方、浓度、防治次数、时期筛选及病斑不同刮治程度试验,2000—2002年在富士苹果枝干轮纹病树上开展果实轮纹病防治试验,结果表明,5波美度石硫合剂∶硅石粉剂=100∶4或100∶6对苹果枝干轮纹病具有显著的增效作用;1年2次施药效果显著;第1次施药前应采取轻刮,以后防治直接用药;多菌灵与波尔多液交替、多菌灵与石硫合剂∶硅石粉剂交替使用采收期与贮藏期防治果实轮纹病效果均突出;套袋果实轮纹病防效达100%。  相似文献   

温度和GA_3对桃果实采后乙烯合成及桃ETR1同源基因表达的影响   总被引：2，自引：0，他引：2  

谢永红  刘国杰  欧毅  吕斌  丁志祥 《果树学报》2006,23(4):523-526

为明确桃果实采后成熟衰老期间乙烯的合成与桃ETR1同源基因之间的关系,进一步了解乙烯的转导和作用过程,设计合成了桃ETR1扩增引物RTetr1和RTetr2,用RT-PCR方法研究了温度和GA3处理对白凤桃(Amyg-daluspersicaL.)果实采后乙烯生成和桃ETR1同源基因表达的影响。结果表明,低温处理降低了果实乙烯释放量,低温+GA3处理不仅降低了果实乙烯释放量,还延缓乙烯释放高峰期。PCR分析表明,桃ETR1的cDNA合成受温度和GA3调节,随着乙烯合成能力的增强,ETR1同源基因的表达水平增加,在乙烯信号转导过程中呈正向表达模式。  相似文献   

赤霞珠葡萄主要品质指标形成规律的研究   总被引：1，自引：0，他引：1  

齐秀东  徐继忠  齐永顺 《河北果树》2006,(4):4-5

在昌黎县酿酒葡萄赤霞珠栽培示范区,对赤霞珠葡萄主要品质指标形成规律进行了研究。测定分析了赤霞珠葡萄成熟过程中,可溶性固形物、糖、酸、果皮中单宁和花青素含量的变化规律。  相似文献