Protective efficacy of stockpiled vaccine against H5N8 highly pathogenic avian influenza virus isolated from a chicken in Kumamoto prefecture,Japan, in 2014     

Koichiro GAMOH  Mari NAKAMIZO  Masatoshi OKAMATSU  Yoshihiro SAKODA  Hiroshi KIDA  Shoko SUZUKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):139-142

H5 highly pathogenic avian influenza (HPAI) viruses have spread worldwide, and antigenic variants of different clades have been selected. In this study, the national stockpiled vaccine prepared from A/duck/Hokkaido/Vac-1/2004 (H5N1) strain was evaluated for the protective efficacy against H5N8 HPAI virus isolated in Kumamoto prefecture, Japan, in April 2014. In the challenge test, all of the vaccinated chickens survived without showing any clinical signs and reduced virus shedding. It was concluded that the present stockpiled vaccine was effective against the H5N8 HPAI virus.  相似文献   

Relationship between formation of earlywood vessels and leaf phenology in two ring-porous hardwoods,Quercus serrata and Robinia pseudoacacia,in early spring     

Kayo Kudo  Koh Yasue  Yoshihiro Hosoo  Ryo Funada 《Journal of Wood Science》2015,61(5):455-464

  相似文献   

Method for estimation of stem carbon fixation of Japanese black pine by combining stem analysis and soft X-ray densitometry     

Seishiro Taki  Yoshihiro Nobori  Maximo Larry Lopez Caceres 《Journal of Forest Research》2014,19(1):226-232

We derived a formula for estimating the relationship between stem carbon weight and stem volume, which was calculated from DBH and tree height using a combination of stem analysis and soft X-ray densitometry. The results indicate carbon weight in a 33-year-old coastal Japanese black pine (Pinus thunbergii) forest is approximately 68,186 kg ha^?1 in Yamagata Prefecture and 38,253 kg ha^?1in a 42-year-old black pine forest in Hokkaido Prefecture, Japan. Also, age-related changes in the stem density following oven-drying of samples of black pine trees are small: the oven-dried density (hereafter “density”) of black pine trees in the two locations mentioned above were 425.6 (kg m^?3) and 523.2 (kg m^?3) respectively, which is comparable to the density (converted from basic density) of black pine of Land Use, Land-Use Change and Forestry (LULUCF) (533 kg m^?3). When compared with the carbon weight by the oven-dried density of LULUCF, the carbon weights calculated from each density were 27 % lower in Yamagata and 6 % lower in Hokkaido. This difference directly affects carbon weight for large-scale estimation and thus can create an error at a regional scale. This methodology can contribute to the management of forests acting as carbon sinks.  相似文献   

Field Distribution of END Phenomenon-Negative Bovine Viral Diarrhea Virus     

Kaoru NISHINE  Hiroshi AOKI  Yoshihiro SAKODA  Akio FUKUSHO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(12):1635-1639

Field isolates of BVDV which do not show the exaltation of Newcastle disease virus (END) phenomenon (END^–) are rarely reported. In this study, 45 BVDV field isolates from cattle in Hokkaido prefecture in Japan were analyzed by the reverse plaque formation method, the END method and observation of cytopathic effects. END^– virus was detected in 34 of 45 isolates (75.6%), although 35 of 45 field isolates contained END phenomenon positive virus as the predominant virus population. We propose that END^– viruses are widely distributed in the field and that it is possible that the mixture of biologically distinct BVDV correlates with the appearance of disease in infected animals.  相似文献   

Pathological and Immunohistochemical Findings of Natural Highly Pathogenic Avian Influenza Infection in Tufted Ducks during 2010–2011 Outbreaks in Japan     

Walied ABDO  Mohie HARIDY  Yuki KATOU  Minami GOTO  Toshio MIZOGUCHI  Yoshihiro SAKODA  Hiroki SAKAI  Tokuma YANAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(9):1285-1290

  相似文献   

Unpredictable Chronic Stress-Induced Reproductive Suppression Associated with the Decrease of Kisspeptin Immunoreactivity in Male Mice     

Tetsushi HIRANO  Yoshihiro KOBAYASHI  Takuya OMOTEHARA  Atsutoshi TATSUMI  Rie HASHIMOTO  Yuria UMEMURA  Daichi NAGAHARA  Youhei MANTANI  Toshifumi YOKOYAMA  Hiroshi KITAGAWA  Nobuhiko HOSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(9):1201-1208

  相似文献   

Multiple high-throughput analyses monitor the response of E. coli to perturbations     

Ishii N  Nakahigashi K  Baba T  Robert M  Soga T  Kanai A  Hirasawa T  Naba M  Hirai K  Hoque A  Ho PY  Kakazu Y  Sugawara K  Igarashi S  Harada S  Masuda T  Sugiyama N  Togashi T  Hasegawa M  Takai Y  Yugi K  Arakawa K  Iwata N  Toya Y  Nakayama Y  Nishioka T  Shimizu K  Mori H  Tomita M 《Science (New York, N.Y.)》2007,316(5824):593-597

Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response of Escherichia coli cells to genetic and environmental perturbations. Analysis of metabolic enzyme gene disruptants revealed unexpectedly small changes in messenger RNA and proteins for most disruptants. Overall, metabolite levels were also stable, reflecting the rerouting of fluxes in the metabolic network. In contrast, E. coli actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. E. coli thus seems to use complementary strategies that result in a metabolic network robust against perturbations.  相似文献   

Negative feedback regulation ensures the one receptor-one olfactory neuron rule in mouse   总被引：1，自引：0，他引：1  

Serizawa S  Miyamichi K  Nakatani H  Suzuki M  Saito M  Yoshihara Y  Sakano H 《Science (New York, N.Y.)》2003,302(5653):2088-2094

In the mouse olfactory system, each olfactory sensory neuron (OSN) expresses only one odorant receptor (OR) gene in a monoallelic and mutually exclusive manner. Such expression forms the genetic basis for OR-instructed axonal projection of OSNs to the olfactory bulb of the brain during development. Here, we identify an upstream cis-acting DNA region that activates the OR gene cluster in mouse and allows the expression of only one OR gene within the cluster. Deletion of the coding region of the expressed OR gene or a naturally occurring frame-shift mutation allows a second OR gene to be expressed. We propose that stochastic activation of only one OR gene within the cluster and negative feedback regulation by that OR gene product are necessary to ensure the one receptor-one neuron rule.  相似文献   

Protein synthesis and neurotrophin-dependent structural plasticity of single dendritic spines     

Tanaka J  Horiike Y  Matsuzaki M  Miyazaki T  Ellis-Davies GC  Kasai H 《Science (New York, N.Y.)》2008,319(5870):1683-1687

Long-term potentiation (LTP) at glutamatergic synapses is considered to underlie learning and memory and is associated with the enlargement of dendritic spines. Because the consolidation of memory and LTP require protein synthesis, it is important to clarify how protein synthesis affects spine enlargement. In rat brain slices, the repetitive pairing of postsynaptic spikes and two-photon uncaging of glutamate at single spines (a spike-timing protocol) produced both immediate and gradual phases of spine enlargement in CA1 pyramidal neurons. The gradual enlargement was strongly dependent on protein synthesis and brain-derived neurotrophic factor (BDNF) action, often associated with spine twitching, and was induced specifically at the spines that were immediately enlarged by the synaptic stimulation. Thus, this spike-timing protocol is an efficient trigger for BDNF secretion and induces protein synthesis-dependent long-term enlargement at the level of single spines.  相似文献   

The rotational spectrum and structure of the HOOO radical     

Suma K  Sumiyoshi Y  Endo Y 《Science (New York, N.Y.)》2005,308(5730):1885-1886

The adduct of the hydroxyl radical with oxygen has been studied theoretically, in connection with atmospheric reactions, but its stability and structure remained an open question. Pure rotational spectra of the HOOO and DOOO radicals have now been observed in a supersonic jet by using a Fourier-transform microwave spectrometer with a pulsed discharge nozzle. The molecular constants extracted from 12 rotational transitions with fine and hyperfine splittings support a trans planar molecular structure, in contrast to the cis planar structure predicted by most ab initio calculations. The bond linking the HO and O2 moieties is fairly long (1.688 angstroms) and comparable to the F-O bond in the isoelectronic FOO radical.  相似文献