Characterization of collagen and its hydrolysate from yellowfin tuna Thunnus albacares skin and their potencies as antioxidant and antiglycation agents     

Nurilmala  Mala  Pertiwi  Rizsa Mustika  Nurhayati  Tati  Fauzi  Shita  Batubara  Irmanida  Ochiai  Yoshihiro 《Fisheries Science》2019,85(3):591-599

Fisheries Science - Collagen is one of the biopolymer proteins that may be useful for food and non-food purposes. Recently, even for collagen, it is becoming necessary to meet the requirements of...  相似文献   

Interannual variation in quantitative relationships among egg production and densities of larvae and juveniles of the Japanese mantis shrimp <Emphasis Type="Italic">Oratosquilla oratoria</Emphasis> in Tokyo Bay,Japan     

Keita Kodama  Masaaki Oyama  Jeong-Hoon Lee  Yoshiaki Akaba  Yoshihiro Tajima  Takamichi Shimizu  Hiroaki Shiraishi  Toshihiro Horiguchi 《Fisheries Science》2009,75(4):875-886

To explore which lifestages affect the stock size of young-of-the-year mantis shrimp Oratosquilla oratoria in Tokyo Bay, Japan, we investigated interannual variations in the quantitative relationships among egg production, larval density, and juvenile density. We collected adult females, larvae, and juveniles during monthly field surveys from 2004 to 2007. The interannual trend for the juvenile density index differed from those for egg production and larval density; although indices of both egg production and larval density were high in 2004 and 2007, the juvenile density index was high only in 2007, suggesting high mortality during the pelagic larval stage or the early phase of the postsettlement juvenile stage in 2004. We found that larval settlement started at the end of August and peaked in October, although larvae from the early spawning season (May–June) should have settled in August or earlier. Juveniles were found throughout the bay except in areas where bottom hypoxia occurred, suggesting that hypoxia restricts the spatial distribution of juveniles. Our results suggest that mortality during the early life history fluctuates among years, probably because of changes in environmental conditions in the bay, resulting in interannual variation in the stock size of young-of-the-year juvenile O. oratoria.  相似文献   

Structural and thermodynamic characterization of tropomyosin from fast skeletal muscle of bluefin tuna   总被引：1，自引：0，他引：1  

Ming-Chih  HUANG  Yoshihiro  OCHIAI  Shugo  WATABE 《Fisheries Science》2004,70(4):667-674

ABSTRACT:   Tuna tropomyosin is a mixture of nearly equimolar amounts of two isoforms (designated α and β). cDNA encoding the α form was cloned from bluefin tuna Thunnus thynnus fast skeletal muscle. The full-length cDNA contained 1220 bp, comprising an open reading frame of 855 bp encoding 284 amino acid residues, flanked by 5'-untranslational regions (156 bp) and 3'-untranslational regions (209 bp). The deduced amino acid sequence showed considerably high homology in a range of 93.7–98.6% to those of other vertebrate α-type tropomyosins. In phylogenetic analysis, bluefin tuna tropomyosin showed the closest relationship with the white croaker counterpart. The predicted mass was 32 919 Da, and isoelectric point was 4.50, assuming acetylation of the N-terminus. By differential scanning calorimetry, bluefin tuna tropomyosin gave two major endothermic peaks at 29.3 and 41.5°C, probably caused by the presence of two isoforms. Circular dichroism spectra supported such a unique denaturation profile.  相似文献   

Precursor B-1 B cell lymphoma in a newborn calf.     

Shinji Yamamoto  Yoshihiro Wada  Yoshiharu Ishikawa  Koichi Kadota 《Journal of veterinary diagnostic investigation》2007,19(4):447-450

A newborn Holstein female calf had neoplastic lesions in the skin and within the thoracic and abdominal cavities but not in the bone marrow, spleen, thymus, or most lymph nodes. Because the tumor cells were positive for CD79a (B cell marker), CD5 (B-1 cell marker) and terminal deoxynucleotidyl transferase (marker for immature lymphoid precursors), a diagnosis of precursor B-1 B cell lymphoma was made. The diagnosis was strongly supported by the fact that B-1 cells can develop in the fetus, unlike B-2 cells, which are produced after birth. The lymphoma was distinct from the typical calf form of lymphoma of B-2 cell origin, which does not express CD5 and is characterized by generalized lymphadenopathy and involvement of the bone marrow, blood and spleen.  相似文献   

Factors affecting the growth of in vitro cultured lateral buds from Phalaenopsis flower stalks     

Michio Tanaka  Yoshihiro Sakanishi 《Scientia Horticulturae》1978,8(2):169-178

Phalaenopsis flower-stalk buds cultured in vitro show 3 modes of growth: dormant, vegetative and reproductive. The effects of bud position on the stalk, temperature, and benzyladenine (BA) on the mode of bud growth were examined.Flower stalks were cut into 1-node sections, each with 1 bud, and inserted into solid culture medium, top side up. Buds on the upper sections had a tendency to remain dormant regardless of temperature. Sprouting buds placed at 20°C or 25°C showed reproductive growth except for some on the basal sections which grew vegetatively. At 28°C, all buds developed vegetatively independent of their original position on the stalk. The buds on the sections taken from both stalks which had elongated at min 18°C and min 28°C were subcultured; the mode of growth of the cultured buds was not affected by the temperature during flower-stalk elongation but was affected by the subsequent culture temperature.Cultured buds which had remained dormant were stimulated to sprout by addition of BA to the medium. At 5 p.p.m. and above, all buds began to develop, but malformations developed on the leaves of the shoots. All factors considered, BA at 2.5 p.p.m. was found to be the optimum level. The further development of the buds released from dormancy by BA was also affected by the position on the flower stalk and by cultural temperature.  相似文献   

Inhibition of acetate ester biosynthesis in banana (Musa sapientum L.) fruit pulp under anaerobic conditions     

Wendakoon SK  Ueda Y  Imahori Y  Ishimaru M 《Journal of agricultural and food chemistry》2004,52(6):1615-1620

The effect of anaerobic conditions on acetate ester biosynthesis in ripened banana pulp was investigated. Incubation of the pulp in less than 1% O(2) resulted in a significant reduction in the formation of ethyl acetate. Regardless of the presence of a large amount of endogenous ethanol and the remaining exogenous isobutyl alcohol after complete anaerobic incubation with the pulp, the production of acetate ester decreased. The effect of addition of pyruvate, isobutyl alcohol, acetate, and methyl hexanoate on acetate ester formation in 100% N(2) was also investigated. The addition of pyruvate and isobutyl alcohol to the pulp gave lower acetate esters in N(2) than in air, whereas the pulp incubated with acetate and isobutyl alcohol produced more acetate ester in both conditions. Therefore, the lack of acetyl CoA, or more precisely acetate, in the tissue is the main reason for the inhibition of acetate ester formation under anaerobic conditions. The activity of beta-oxidation measured by incubation with methyl hexanoate was detected only in the samples incubated in air. The formation of acetyl CoA, derived from pyruvate through mitochondria and through beta-oxidation, was inhibited by anaerobic conditions, which suggests that mitochondrial activity and/or beta-oxidation are essential for ester biosynthesis.  相似文献   

Geographical and functional-morphological variations of the skull in the gray-bellied squirrel     

Endo H  Kimura J  Oshida T  Stafford BJ  Rerkamnuaychoke W  Nishida T  Sasaki M  Hayashida A  Hayashi Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(3):277-282

The geographical variations of the skulls were osteometrically examined in the gray-bellied squirrel (Callosciurus caniceps) from the populations of Korat, Ranong, southernmost Thailand, and Terutau Island. The skull size was larger in northern population than in the southern population in the continental mainland. The zoogeographical influences of the Isthmus of Kra remained unclear, since the plots from Korat population were intermingled with those from southernmost Thailand population in the principal component charts. Although Korat population has been thought to belong to north group, we suggest that Ranong and southernmost Thailand populations may contain individuals from both north and south groups separated by the ancient Kra barrier. Terutau Island population was similar to southernmost Thailand population in skull size, although Terutau population has been isolated in the island and separated from the south group of the Isthmus of Kra. In the proportional analysis the interorbital space was narrower and the binocular sense has been well-developed in Terutau population. It suggests that this population has been highly adapted to arboreal behavior. In contrast, the skull with larger interorbital space was more adaptive for terrestrial life in Korat population. The canonical discriminant analysis could clearly separate the four populations in the scattergrams of discriminant scores.  相似文献   

Geographical variation of skull size and shape in various populations in the black giant squirrel     

Endo H  Kimura J  Oshida T  Stafford BJ  Rerkamnuaychoke W  Nishida T  Sasaki M  Hayashida A  Hayashi Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(10):1213-1218

We osteometrically examined the skulls of the black giant squirrel (Ratufa bicolor) from three mainland populations (M. Malayan Peninsula, V. South Vietnam, and B. Burma, India and North Thailand) and from two island populations (T. Tioman, and S. Sumatra Islands). The skull in the Malayan peninsula population was significantly smaller than that of the two other mainland populations. It is consistent with Bergmann's rule as shown in the gray-bellied squirrel. The two island populations did not show obvious differences in comparison with the Malayan population in many measurements. In the proportion analysis eliminating the size factor, the differences among populations were not easily confirmed and we concluded that the osteological characters peculiar to each population could not be shown in this species. The first and second principal component scores of M, S, and T populations were intermingled, whereas the V and B populations of V and B were not separated in the chart. We pointed out that the morphological differences were demonstrated between northern and southern groups of the Isthmus of Kra in the mainland populations, and that the two island populations did not show the island-isolation effect in comparison with the M population. The adaptational variation related to feeding and locomotion could not be confirmed among populations of the black giant squirrel as shown in the proportion analysis.  相似文献   

High level activity of 2', 5'-oligoadenylate synthetase in dog serum     

Iwata A  Yamamoto A  Fujino M  Sato I  Hosokawa-Kanai T  Tuchiya K  Ishihama A  Sokawa Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(6):721-724

Most animal cells that are exposed to interferon (IFN) experience an increase in the activity of 2', 5'-oligoadenylate synthetase (OAS), which is an important effector of IFN's antiviral action. OAS activity has been widely used in clinical chemistry as an indicator of IFN activity. In this study, we found that OAS activity in canine serum is 46.0 +/- 40.4 nmol/dl/hr, which is 10- to 100-fold higher than in other animals such as the cat (1.9 +/- 2.1), rabbit (4.0 +/- 1.1), and guinea pig (0.3 +/- 0.6). The canine OAS protein was detected by Western blotting using a 68M-10 monoclonal anti-murine OAS antibody, and was found to be composed of at least three distinct molecular species of p40 class OAS. Among these, the 40 and 42 kDa components were determined to be the major species in serum and fibroblast cell lines, respectively.  相似文献   

Expression of Prnp mRNA (prion protein gene) in mouse spermatogenic cells     

Fujisawa M  Kanai Y  Nam SY  Maeda S  Nakamuta N  Kano K  Kurohmaru M  Hayashi Y 《The Journal of reproduction and development》2004,50(5):565-570

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