Identification of Japanese strains of<Emphasis Type="Italic"> Barley yellow mosaic virus</Emphasis> by RT-PCR/RFLP analysis of the coat protein-coding region     

Yoshihiro Okada  Tsuneo Kato  Masahiro Yoshida  Akira Saito 《Journal of General Plant Pathology》2008,74(3):258-263

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Molecular species of collagen in muscular and vertebral parts of white sturgeon Acipenser transmontanus     

Mizuta  Shoshi  Asano  Chisato  Yokoyama  Yoshihiro  Taniguchi  Motoki 《Fisheries Science》2012,78(2):399-406

The molecular species of collagen in the muscular and vertebral parts of white sturgeon Acipenser transmontanus were examined by biochemical techniques. Pepsin-solubilized collagens were prepared from these parts, and fractionated into major and minor collagen fractions by differential salt precipitation at acidic or neutral pH. Collagens contained in these fractions showed several features characteristic of fish type I and V collagens, respectively, in SDS-PAGE patterns and the amino acid compositions of both parts. These results suggest that at least two molecular species, corresponding to type I and V collagens, are distributed in the muscular and vertebral parts of white sturgeon.  相似文献   

A general solution technique for electroelastic fields in piezoelectric bodies with D∞ symmetry in cylindrical coordinates     

Masayuki Ishihara  Yoshihiro Ootao  Yoshitaka Kameo 《Journal of Wood Science》2016,62(1):29-41

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Identification of introgression lines of Oryza glaberrima Steud. with high mineral content in grains     

Yoshihiro Ohmori  Naoyuki Sotta 《Soil Science and Plant Nutrition》2016,62(5-6):456-464

Mineral concentrations in cereals are crucial for human health, especially for people who consume cereals as a subsistence diet. Although rice (Oryza sativa L.) is one of most important staple crops in the world, starch constitutes a large portion of its grains, and mineral concentrations are lower than in other staple crops. One solution is the improvement of mineral concentrations in rice grains through biofortification, and this approach requires investigation of genetic resources that confer high mineral concentrations to rice grains. In the present study, we performed 2 years of field experiments, in 2012 and 2013, and determined sodium (Na), magnesium (Mg), phosphorus (P), potassium (K), calcium (Ca), boron (B), manganese (Mn), iron (Fe), copper (Cu), zinc (Zn) and molybdenum (Mo) concentrations in the rice grains of 40 introgression lines derived from a cross between a japonica cultivar, Taichung 65 (T65), and African rice, Oryza glaberrima Steud. Substantial variation in mineral concentrations was observed among the 40 introgression lines. We selected several elite lines that had significantly higher concentrations of minerals, including Fe, Cu, Zn, Mo, Mg, P and Ca than the cultivated rice T65. These lines could be novel potential materials for breeding programs for biofortification and provide us with positional information for the candidate loci in the O. glaberrima genome responsible for high mineral concentrations in rice grains.  相似文献   

Genomic survey of polymorphisms in pattern recognition receptors and their possible relationship to infections in pigs     

Uenishi H  Shinkai H  Morozumi T  Muneta Y 《Veterinary immunology and immunopathology》2012,148(1-2):69-73

Recent progress in the accumulation of pig genomic information has enabled us to comprehensively explore polymorphisms in pig genes. One of our targets for exploration has been the genes encoding molecules related to pathogen recognition, such as pattern recognition receptors (PRRs). PRRs play a role in the innate immune system, and possess various members such as Toll-like receptors (TLRs), NOD-like receptors (NLRs), RIG-like helicases (RLHs), and C-type lectin-like receptors (CLRs). PRRs are required for the monitoring of pathogens; therefore, polymorphisms in PRRs may influence molecular functions such as ligand recognition. There have been many studies on the relationship between polymorphisms within PRR genes and disease susceptibility in humans and mice. Our studies have revealed that porcine PRR genes possess many nonsynonymous polymorphisms, particularly in regions encoding the ectodomains of TLRs localized on the cell surface. The genes encoding TLRs located on the membrane of intracellular compartments, and cytoplasmic PRRs such as NLRs and RLHs, also possessed nonsynonymous polymorphisms. Several observations indicate that there are relationships between polymorphisms in PRR or related genes and disease susceptibility in livestock animals including pig. Such information may contribute to breeding aimed at disease resistance, and effective vaccine design.  相似文献   

Snapshots Analyses for Turbidity Measurements in Water     

Antonio Cesar Godoy  Alberto Yoshihiro Nakano  David Antônio Brum Siepmann  Ricardo Schneider  Felipe Walter Dafico Pfrimer  Oscar Oliveira Santos 《Water, air, and soil pollution》2018,229(12):378

Turbidity has been used as an effective indicator of water quality and it is regulated by national control agencies as a water potability parameter. This work proposes a simple, low-cost, and easily reproducible method based on digitally processed snapshots of electromagnetic radiation beam through a scattering medium in order to measure turbidity. The formazin polymer was used as the standard reference in the experiments to prepare samples from 0.1 to 100 NTU for high turbidity range and from 0.02 to 10 FNU for low turbidity range. The device design is comprised of a webcam and an LED/laser as a light sensor and a radiation source, respectively. The captured scattered light snapshot can be decomposed digitally in color components values and correlated with the turbidity parameter. It is possible to obtain differently device performances changing the light sensor device configuration. The linear regression analyses have shown a distinct relationship among red, green, blue, and grayscale components and the turbidity. At high range, the green component present the best LOD and LOQ values 0.64 and 2.10, respectively, operating with an ordinary webcam and white LED. Nevertheless, the best device performances were obtained with dedicated Raspberry Pi camera modules and white LED for low range turbidity reaching LOD=0.027 and LOQ=0.087 FNU. The figures of merit show recovery between (97.50?101.95%), repeatability (2.11%), within-laboratory reproducibility (2.25%), and limits of quantification (0.087 FNU). The achieved performance device shows the effectiveness of digitally processed snapshots obtained with digital cameras for turbidity measurements.  相似文献   

Oceanographic factors affecting interannual recruitment variability of Pacific saury (Cololabis saira) in the central and western North Pacific          下载免费PDF全文

Taro Ichii  Haruka Nishikawa  Kedarnath Mahapatra  Hiroshi Okamura  Hiromichi Igarashi  Mitsuo Sakai  Satoshi Suyama  Masayasu Nakagami  Miyako Naya  Norihisa Usui  Yoshihiro Okada 《Fisheries Oceanography》2018,27(5):445-457

Pacific saury (Cololabis saira) has a short life span of 2 years and tends to exhibit marked population fluctuations. To examine the importance of sea surface temperature (SST) and mixed layer depth (MLD) as oceanographic factors for interannual variability of saury recruitment in early life history, we analyzed the relationship between abundance index (survey CPUE (catch per unit of effort)) of age‐1 fish and the oceanographic factors in the spawning and nursery grounds of the previous year when they were born, for the period of 1979–2006, in the central and western North Pacific. Applying the mixture of two linear regression models, the variability in the survey CPUE was positively correlated with previous year's winter SST in the Kuroshio Recirculation region (KR) throughout the survey period except 1994–2002. In contrast, the survey CPUE was positively correlated with the previous year's spring MLD (a proxy of spring chlorophyll a (Chl‐a) concentration) in the Kuroshio‐Oyashio Transition and Kuroshio Extension (TKE) during 1994–2002. This period is characterized by unusually deep spring MLD during 1994–1997 and anomalous climate conditions during 1998–2002. We suggest that saury recruitment variability was generally driven by the winter SST in the KR (winter spawning/nursery ground), or by the spring Chl‐a concentration (a proxy of prey for saury larvae) in the TKE (spring spawning/nursery ground). These oceanographic factors could be potentially useful to predict abundance trends of age‐1 saury in the future if the conditions leading to the switch between SST and MLD as the key input variable are elucidated further.  相似文献   

Cetacean Toll-like receptor 4 and myeloid differentiation factor 2, and possible cetacean-specific responses against Gram-negative bacteria     

Reiko Shishido  Kazue Ohishi  Rintaro Suzuki  Kiyotaka Takishita  Dai Ohtsu  Kenji Okutsu  Koji Tokutake  Etsuko Katsumata  Takeharu Bando  Yoshihiro Fujise  Tsukasa Murayama  Tadashi Maruyama   《Comparative immunology, microbiology and infectious diseases》2010,33(6):e89-e98

Toll-like receptor 4 (TLR4) and myeloid differentiation factor 2 (MD-2) are essential for recognizing the lipopolysaccharides (LPS) of Gram-negative bacteria. We determined the sequences of cDNAs encoding TLR4 and MD-2 from cetaceans and generated three-dimensional (3D) models for a better understanding of their modes of interaction and LPS recognition. The 3D reconstructions showed that cetacean TLR4 and MD-2 formed a horseshoe-like structure comprised of parallel β-strands and a β-cup structure consisting of two anti-parallel β-sheets, respectively. The (TLR4-MD-2)₂ duplex-heterodimer was shown to form a symmetrical structure. Comparison with the interfaces of the complexes in other mammals revealed that cetacean TLR4s have some amino acid residue substitutions involved in duplex-heterodimer formation and in species variation for LPS recognition. These substitutions in the changed amino acid residues may alter the interaction among TLR4, MD-2, and LPS and modify the TLR4/MD-2 immunological responses.  相似文献   

Smenospongine,a Sesquiterpene Aminoquinone from a Marine Sponge,Induces G1 Arrest or Apoptosis in Different Leukemia Cells          下载免费PDF全文

Dexin Kong  Shunji Aoki  Yoshihiro Sowa  Toshiyuki Sakai  Motomasa Kobayashi 《Marine drugs》2008,6(3):480-488

Smenospongine, a sesquiterpene aminoquinone isolated from the marine sponge Dactylospongia elegans, was previously reported by us to induce erythroid differentiation and G1 phase arrest of K562 chronic myelogenous leukemia cells. In this study, we investigated the effect of smenospongine on the cell cycles of other leukemia cells, including HL60 human acute promyelocytic leukemia cells and U937 human histiocytic lymphoma cells by flow cytometric analysis. Smenospongine induced apoptosis dose-dependently in HL60 and U937 cells. The smenospongine treatment increased expression of p21 and inhibited phosphorylation of Rb in K562 cells, suggesting the p21-Rb pathway play an important role in G1 arrest in K562 cells. However, the p21 promoter was not activated by the smenospongine treatment based on a luciferase assay using the transfected K562 cells. Smenospongine might induce p21 expression via another mechanism than transactivation of p21 promoter.  相似文献   

Elimination of the major allergen tropomyosin from shrimp muscle by boiling treatment     

Ozawa  Hideo  Yamamura  Akira  Kimijima  Takeo  Ishizaki  Shoichiro  Ochiai  Yoshihiro 《Fisheries Science》2020,86(1):197-202

Fisheries Science - Tropomyosin (TM) is the major allergen found in invertebrates, including shrimp. To reduce the allergenicity of shrimp muscle, attempts were made to eliminate TM from the muscle...  相似文献