Elimination of the major allergen tropomyosin from shrimp muscle by boiling treatment     

Ozawa  Hideo  Yamamura  Akira  Kimijima  Takeo  Ishizaki  Shoichiro  Ochiai  Yoshihiro 《Fisheries Science》2020,86(1):197-202

Fisheries Science - Tropomyosin (TM) is the major allergen found in invertebrates, including shrimp. To reduce the allergenicity of shrimp muscle, attempts were made to eliminate TM from the muscle...  相似文献   

Characterization of collagen and its hydrolysate from yellowfin tuna Thunnus albacares skin and their potencies as antioxidant and antiglycation agents     

Nurilmala  Mala  Pertiwi  Rizsa Mustika  Nurhayati  Tati  Fauzi  Shita  Batubara  Irmanida  Ochiai  Yoshihiro 《Fisheries Science》2019,85(3):591-599

Fisheries Science - Collagen is one of the biopolymer proteins that may be useful for food and non-food purposes. Recently, even for collagen, it is becoming necessary to meet the requirements of...  相似文献   

Interannual variation in quantitative relationships among egg production and densities of larvae and juveniles of the Japanese mantis shrimp <Emphasis Type="Italic">Oratosquilla oratoria</Emphasis> in Tokyo Bay,Japan     

Keita Kodama  Masaaki Oyama  Jeong-Hoon Lee  Yoshiaki Akaba  Yoshihiro Tajima  Takamichi Shimizu  Hiroaki Shiraishi  Toshihiro Horiguchi 《Fisheries Science》2009,75(4):875-886

To explore which lifestages affect the stock size of young-of-the-year mantis shrimp Oratosquilla oratoria in Tokyo Bay, Japan, we investigated interannual variations in the quantitative relationships among egg production, larval density, and juvenile density. We collected adult females, larvae, and juveniles during monthly field surveys from 2004 to 2007. The interannual trend for the juvenile density index differed from those for egg production and larval density; although indices of both egg production and larval density were high in 2004 and 2007, the juvenile density index was high only in 2007, suggesting high mortality during the pelagic larval stage or the early phase of the postsettlement juvenile stage in 2004. We found that larval settlement started at the end of August and peaked in October, although larvae from the early spawning season (May–June) should have settled in August or earlier. Juveniles were found throughout the bay except in areas where bottom hypoxia occurred, suggesting that hypoxia restricts the spatial distribution of juveniles. Our results suggest that mortality during the early life history fluctuates among years, probably because of changes in environmental conditions in the bay, resulting in interannual variation in the stock size of young-of-the-year juvenile O. oratoria.  相似文献   

Endemic infections of bovine viral diarrhea virus genotypes 1b and 2a isolated from cattle in Japan between 2014 and 2020     

Asami NISHIMORI  Shizuka HIROSE  Saho OGINO  Kiyohiko ANDOH  Norikazu ISODA  Yoshihiro SAKODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(2):228

Bovine viral diarrhea virus (BVDV) is a causative agent of bovine viral diarrhea. In Japan, a previous study reported that subgenotype 1b viruses were predominant until 2014. Because there is little information regarding the recent epidemiological status of BVDV circulating in Japan, we performed genetic characterization of 909 BVDV isolates obtained between 2014 and 2020. We found that 657 and 252 isolates were classified as BVDV-1 and BVDV-2, respectively, and that they were further subdivided into 1a (35 isolates, 3.9%), 1b (588, 64.7%), 1c (34, 3.7%), and 2a (252, 27.7%). Phylogenetic analysis using entire E2 coding sequence revealed that a major domestic cluster in Japan among BVDV-1b and 2a viruses were unchanged from a previous study conducted from 2006 to 2014. These results provide updated information concerning the epidemic strain of BVDV in Japan, which would be helpful for appropriate vaccine selection.  相似文献   

Structural and thermodynamic characterization of tropomyosin from fast skeletal muscle of bluefin tuna   总被引：1，自引：0，他引：1  

Ming-Chih  HUANG  Yoshihiro  OCHIAI  Shugo  WATABE 《Fisheries Science》2004,70(4):667-674

ABSTRACT:   Tuna tropomyosin is a mixture of nearly equimolar amounts of two isoforms (designated α and β). cDNA encoding the α form was cloned from bluefin tuna Thunnus thynnus fast skeletal muscle. The full-length cDNA contained 1220 bp, comprising an open reading frame of 855 bp encoding 284 amino acid residues, flanked by 5'-untranslational regions (156 bp) and 3'-untranslational regions (209 bp). The deduced amino acid sequence showed considerably high homology in a range of 93.7–98.6% to those of other vertebrate α-type tropomyosins. In phylogenetic analysis, bluefin tuna tropomyosin showed the closest relationship with the white croaker counterpart. The predicted mass was 32 919 Da, and isoelectric point was 4.50, assuming acetylation of the N-terminus. By differential scanning calorimetry, bluefin tuna tropomyosin gave two major endothermic peaks at 29.3 and 41.5°C, probably caused by the presence of two isoforms. Circular dichroism spectra supported such a unique denaturation profile.  相似文献   

Effects of chitin and chitosan on collagen synthesis in wound healing   总被引：7，自引：0，他引：7  

Kojima K  Okamoto Y  Kojima K  Miyatake K  Fujise H  Shigemasa Y  Minami S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(12):1595-1598

Collagen synthesis was evaluated by measuring prolyl hydroxylase (PHL) activity induced within rat granulation tissue by a polyester non-woven fabric (NWF, 1 x 1 cm, 0.6 mm in thickness) impregnated with a chitin or chitosan suspension ranging in concentration from 0.1 to 10 mg/ml. In addition, PHL activity induced in rat granulation tissue by a NWF impregnated with a phosphate buffer solution was examined as a control. The PHL activity in each group remained low until 4 days post-implantation (Day 4). However, in the 10 mg chitin group, the PHL activity increased rapidly without scatter of the data at Day 7 and remained at a plateau until Day 14. In other groups, PHL activity increased linearly until Day 14. The data varied widely at Day 7. Compared to chitosan, chitin at the higher concentration was found to induce stable collagen synthesis in the early wound healing process.  相似文献   

Aspergillus fumigatus infection in an ostrich (Struthio camelus)     

Yokota T  Shibahara T  Wada Y  Hiraki R  Ishikawa Y  Kadota K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(2):201-204

An 11-month-old female ostrich (Struthio camelus) had become gradually emaciated over a 2-week period and subsequently died. Necropsy revealed white to green mold growth on the walls of caseous thickened air sac membranes and multiple white necrotic foci in the lungs and liver. Histologically, the multiple exudative, necrotic and granulomatous lesions were compatible with mycotic infection in the air sacs and lungs, and hyphae positively reacted with a monoclonal antibody (Mab-WF-AF-1) to Aspergillus fumigatus wall fractions. Multifocal hepatic necrosis was also found, and several spores were observed in the blood vessels. Fungal culture of these lesions yielded pure growth of A. fumigatus. This is an established case of fatal A. fumigatus infection in an ostrich reared in Japan.  相似文献   

Effect of anti-ganglioside antibody in experimental Trypanosoma brucei infection in mice     

Tsujimura Y  Watarai S  Uemura A  Ohnishi Y  Kodama H 《Research in veterinary science》2005,78(3):245-247

The effect of antibody against ganglioside antigen on Trypanosoma brucei parasites was examined in vitro and in vivo using anti-ganglioside GM1 (AGM-1) monoclonal antibody. The antibody showed complement-dependent cytotoxicity against T. brucei with mouse complement. Furthermore, mice given AGM-1 were challenged intraperitoneally with T. brucei. Although all non-treated control mice died within six days after infection, all of AGM-1-injected mice had survived by six days post-infection. These data suggest that antibody against ganglioside antigen on T. brucei has potential in protection against T. brucei infection.  相似文献   

Development of vaccine strains of H5 and H7 influenza viruses   总被引：1，自引：0，他引：1  

Soda K  Sakoda Y  Isoda N  Kajihara M  Haraguchi Y  Shibuya H  Yoshida H  Sasaki T  Sakamoto R  Saijo K  Hagiwara J  Kida H 《The Japanese journal of veterinary research》2008,55(2-3):93-98

To establish vaccine strains of H5 and H7 influenza viruses, A/duck/Hokkaido/Vac-1/04 (H5N1) [Vac-1/04 (H5N1)], A/duck/Hokkaido/Vac-3/07 (H5N1) [Vac-3/07 (H5N1)], and A/duck/Hokkaido/ Vac-2/04 (H7N7) [Vac-2/04 (H7N7)] were generated from non-pathogenic avian influenza viruses isolated from migratory ducks. Vac-1/04 (H5N1) and Vac-3/07 (H5N1) were generated by genetic reassortment between H5N2 or H5N3 virus as an HA gene provider and H7N1 or H6N1 viruses as an NA gene provider. Vac-2/04 (H7N7) was a genetic reassortant obtained using H7N7 and H9 N2 viruses to give high growth character of the H9N2 virus in chicken embryonated eggs. The results of sequence analyses and experimental infections revealed that these H5N1 and H7N7 reassortant viruses were non-pathogenic in chickens and embryos, and had good growth potential in embryonated eggs. These viruses should be useful to develop vaccines against H5 and H7 highly pathogenic avian influenza viruses.  相似文献   

Pretreatment of macrophages with the combination of IFN-gamma and IL-12 induces resistance to Leishmania major at the early phase of infection     

Ota H  Takashima Y  Matsumoto Y  Hayashi Y  Matsumoto Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(6):589-593

Interferon (IFN)-gamma is essential but not sufficient to control leishmaniasis. It is known that IFN-gamma is one of the major macrophage-activating cytokines, and the activated macrophages are a principal source of interleukin (IL)-12, which induces autocrine macrophage activation. In this study, the combined effect of IFN-gamma and IL-12 on the susceptibility of macrophages to Leishmania major infection was evaluated. Macrophages pretreated with IFN-gamma and/or IL-12 were infected with the parasites. Four hr post-infection (p.i.), the levels of infection and parasite load in the macrophages treated with the combination of IFN-gamma and IL-12 (IFN-gamma/IL-12) were significantly lower than those in the nontreated cells. However, the macrophages treated with either IFN-gamma or IL-12 did not show resistance to L. major infection. In addition, 72 hr p.i., the IFN-gamma/IL-12-treated and IFN-gamma-treated macrophages showed significantly lower levels of infection and parasite load than the nontreated cells, and higher levels of resistance was observed in the IFN-gamma/IL-12-treated macrophages than in the IFN-gamma-treated macrophages. Although IFN-gamma/IL-12 treatment of macrophages prior to the infection led to the induction of resistance, as described above, this resistance was not induced when these cytokines and the parasites were added simultaneously to the macrophage culture. These results suggest that IFN-gamma/IL-12 treatment prior to the infection restricts the early phase of the infection.  相似文献