Molecular typing of Staphylococcus aureus isolated from bovine mastitic milk on the basis of toxin genes and coagulase gene polymorphisms   总被引：3，自引：0，他引：3  

Katsuda K  Hata E  Kobayashi H  Kohmoto M  Kawashima K  Tsunemitsu H  Eguchi M 《Veterinary microbiology》2005,105(3-4):301-305

A total of 270 strains of Staphylococcus aureus, isolated from mastitic milk, were investigated by the polymerase chain reaction for the presence of genes encoding enterotoxins (sea to sej) and a toxic shock syndrome toxin (tst). One hundred eighty three (67.8%) bovine isolates possessed either one or more toxin genes and the most common pattern that coexisted in S. aureus was tst, sec, seg, and sei. Coagulase genotyping revealed 15 patterns, and 161 of the 270 isolates (59.6%) belonged to the coagulase genotype B1. Further, these 161 isolates possessed at least two enterotoxin genes. However, the role of these toxins in udder pathogenicity remains unclear. Moreover, the predominant isolate possessed the enterotoxin genes supporting the theory that superantigenic toxins are important for the udder pathogenesis.  相似文献   

Isolation and expression of five-amino-acid-deleted variant of feline hepatocyte growth factor (HGF) cDNA     

Miyake M  Yaguchi T  Saze K  Wang J  Ogawa T  Endo Y  Suzuta Y  Okazaki M  Haga Y  Waki T  Takahashi SY  Yamamoto Y  Iwabuchi S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(1):9-14

Hepatocyte growth factor (HGF) is a pleiotropic cytokine that stimulates a wide array of cellular targets, including hepatocytes and other epithelial cells, melanocytes, endothelial and hematopoietic cells. We have cloned a different form of cDNA, with a deletion of 15 base pairs predicted to result in the loss of 5 amino acids from the first kringle domain. To investigate the biological activity, original and deleted variant of feline HGF cDNAs were transiently expressed in COS-7 cells. Both recombinant feline HGFs showed almost the same dose-response curves in the stimulation of the growth of BNL CL.2 cells (a mouse hepatocyte cell line) and scatter activity of Madin-Darby canine kidney (MDCK) cells. The findings reported here suggest that the deleted variant of feline HGF has almost the same biological activity as the original in terms of the proliferation and scatter activity.  相似文献   

Accumulation mechanism of γ-aminobutyric acid in tomatoes (Solanum lycopersicum L.) under low O2 with and without CO2     

Mae N  Makino Y  Oshita S  Kawagoe Y  Tanaka A  Aoki K  Kurabayashi A  Akihiro T  Akama K  Koike S  Takayama M  Matsukura C  Ezura H 《Journal of agricultural and food chemistry》2012,60(4):1013-1019

The storage of ripe tomatoes in low-O(2) conditions with and without CO(2) promotes γ-aminobutyric acid (GABA) accumulation. The activities of glutamate decarboxylase (GAD) and α-ketoglutarate-dependent GABA transaminase (GABA-TK) were higher and lower, respectively, following storage under hypoxic (2.4 or 3.5% O(2)) or adjusted aerobic (11% O(2)) conditions compared to the activities in air for 7 days at 25 °C. GAD activity was consistent with the expression level of mRNA for GAD. The GABA concentration in tomatoes stored under hypoxic conditions and adjusted aerobic conditions was 60-90% higher than that when they are stored in air on the same day. These results demonstrate that upregulation of GAD activity and downregulation of GABA-TK activity cause GABA accumulation in tomatoes stored under low-O(2) conditions. Meanwhile, the effect of CO(2) on GABA accumulation is probably minimal.  相似文献   

Impact of awareness of the Japanese Food Guide Spinning Top on eating behaviour     

Takaizumi K  Harada K  Shibata A  Nakamura Y 《Public health nutrition》2012,15(3):399-406

  相似文献   

Relationship between leukocyte population and nutritive conditions in dairy herds with frequently appearing mastitis     

Ohtsuka H  Kohiruimaki M  Hayashi T  Katsuda K  Matsuda K  Masui M  Abe R  Kawamura S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(2):113-118

To clarify the relationship between cellular immune status and nutritive condition, feeding program, blood profiles, and leukocyte populations were analyzed in two dairy herds experiencing frequent mastitis. Fourteen of the 35 lactating cows in herd A, and 18 of the 50 lactating cows in herd B scored positive on the California Mastitis Test (CMT), and 3 of the 73 lactating cows were CMT positive in herd C, which was the control. All herds were evaluated during five different milking stages, and blood was collected from five cows at each stage. With regard to feed content, the percentages of total digestible nutrients (TDN) and crude protein (CP) were found to be lower in herds A and B than in herd C. Levels of serum total cholesterol and blood urea nitrogen were lower in herds A and B than those in herd C. Neutrophil counts in herds A and B were increased compared to the neutrophil counts in herd C. On the other hand, the numbers of CD3(+) T cells and CD14-MHC class(+) cells were lower in herd A and B than in herd C. A decrease in peripheral lymphocytes and undernourishment were observed in the herds with frequent occurring mastitis.  相似文献   

Interspecific cytoplasm substitutions of an Indica strain of Oryza sativa L. and O. Glaberrima Steud     

Yoshio Sano 《Euphytica》1985,34(3):587-592

Summary To study differential nucleus-cytoplasm interactions between the two cultivated rice species, Oryza sativa and O. glaberrima, cytoplasmic substitution lines were made by using a glaberrima strain (G) and an Indica strain of sativa (S). The G cytoplasm had no adverse effect on pollen development when combined with the nucleus of S. On the other hand, when the S cytoplasm was combined with the G nucleus, the substitution line showed no seed set because of male sterility although the pollen grains were normally stained with I₂-KI solution. A dominant gene derived from S strain seemed to cause anther indehiscence in the substitution line. Further, a restorer gene (Rf _j)from Akebono of Japonica type was effective on pollen restoration in the male sterile line, suggesting that the S cytoplasm is the same as those of Japonica type in terms of a fertility-restoring system.This paper is Genetic studies of speciation in cultivated rice. 4.  相似文献   

Behavior of Iodine in a Forest Plot, an Upland Field and a Paddy Field in the Upland Area of Tsukuba, Japan. Iodine Concentration in Precipitation, Irrigation Water, Ponding Water and Soil Water to a Depth of 2.5 m     

Kouichi Yuita  Nobuharu Kihou  Shiho Yabusaki  Yoshio Takahashi  Takayuki Saitoh  Akito Tsumura  Hideki Ichihashi 《Soil Science and Plant Nutrition》2005,51(7):1011-1021

  相似文献   

Molecular differentiation,diversity, and folk classification of “sweet” and “bitter” cassava (<Emphasis Type="Italic">Manihot esculenta</Emphasis>) in Caiçara and Caboclo management systems (Brazil)   总被引：1，自引：0，他引：1  

Nivaldo Peroni  Paulo Yoshio Kageyama  Alpina Begossi 《Genetic Resources and Crop Evolution》2007,54(6):1333-1349

This study focuses the inter-relationships among the men, the use, and the intra-specific cassava diversity, under the perspective of this crop evolutionary dynamics. The origin, the use and the current local management of varieties with high and low cyanogenic potential are important questions around cassava domestication. We collected 169 local varieties identified as “sweet” or “bitter” cassava by traditional farmers from Atlantic Forest and Amazon (Medium Negro River Basin), Brazil. Using a population genetics and an ethnobotany approach, the diversity and the genetic structure of cassava were evaluated. We found a total of 115 vernacular names, and in the Atlantic Forest sample the average genetic diversity (H _S = 0.654) was higher for the sweet varieties than for bitter ones (0.582). The genetic differentiation coefficient (R _ST), used to estimate the diversity among groups, was 0.057 (P < 0.001), indicating that the divergence between the two groups is low. We obtained a low correlation between the morphological and genetic distances, and the congruence was high when the ethnoclassification and the genetic structure were considered. We discuss the adaptive advantages of the sweet varieties use, the current socio-economic changes in bitter varieties use, and the ecological history of these variety groups.  相似文献   

Effects of cyclosporin A on susceptibility and interferon-generating capacity in mice infected with Toxoplasma gondii     

T Shirahata  K Katsuda  C Ohta  H Goto 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(2):387-393

Cyclosporin A (Cs-A), a potent immunosuppressant, was administered to mice to evaluate the role of T lymphocytes for the development of a protective immunity to an obligate intracellular protozoan parasite, Toxoplasma gondii. Although daily administration of various amounts of Cs-A for 7 days enhanced the host susceptibility at all doses employed, a dose-dependent manner of Cs-A treatment was not observed as far as the dosage regimens applied here are concerned; mice died at the same rate (40%) among the groups receiving various amounts of Cs-A. Cs-A treatment had a differential effect on the course of disease depending on how it was given in relation to infection. All mice receiving 50 mg of Cs-A per kg per day for 10 days from the beginning of infection eventually died of toxoplasmosis. Cs-A did not suppress the production of intereferon(IFN)-alpha/beta that was induced shortly after the infection, whereas it reduced greatly the ability of Toxoplasma-infected mice to produce IFN-gamma induced by stimulation with Toxoplasma lysate antigen (TLA). Moreover, the decrease in IFN-gamma production correlated with an increase in the parasitic growth in the peritoneal cavities of Cs-A-treated mice. These results suggest that the immunosuppressive effect of Cs-A on the primary Toxoplasma infection in mice is expressed by inhibiting the development of effector T cells responsible for the production of IFN-gamma.  相似文献   

The effects of acute exposure to deoxynivalenol on some inflammatory parameters in miniature pigs     

Mikami O  Kubo M  Murata H  Muneta Y  Nakajima Y  Miyazaki S  Tanimura N  Katsuda K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(5):665-671

Seven miniature pigs were injected intravenously with deoxynivalenol (DON) at 1 mg/kg body weight; afterward, the number of leukocytes in peripheral blood, the luminol-dependent chemiluminescence of neutrophils, the serum or plasma concentration of cytokines and acute-phase proteins were evaluated to determine the effects of acute exposure to DON on inflammatory responses. White blood cell counts were transiently increased at 3, 6, and 12 hr post-injection (PI) due to the increased number of neutrophils. The luminol-dependent chemiluminescence value of neutrophils was significantly elevated at 24 hr PI, indicating the activation of the bactericidal function of neutrophils. Significant increases of interleukin (IL)-8 and tumor necrosis factor-α at 3 hr PI and IL-6 at 6 hr PI were detected in the serum. The concentration of haptoglobin and serum amyloid A was significantly increased at 24 hr PI. These results suggest that acute exposure to DON induced a temporary recruitment of neutrophils in the peripheral blood by IL-8 and subsequent activation of the bactericidal function, and a transient increase of proinflammatory cytokines and acute-phase proteins, indicating the immunomodulatory effects of DON in pigs.  相似文献