Accessory corpora lutea formation in pregnant Hokkaido sika deer (Cervus nippon yesoensis) investigated by examination of ovarian dynamics and steroid hormone concentrations     

Yojiro YANAGAWA  Yukiko MATSUURA  Masatsugu SUZUKI  Shin-ichi SAGA  Hideto OKUYAMA  Daisuke FUKUI  Gen BANDO  Masashi NAGANO  Seiji KATAGIRI  Yoshiyuki TAKAHASHI  Toshio TSUBOTA 《The Journal of reproduction and development》2015,61(1):61-66

Generally, sika deer conceive a single fetus, but approximately 80% of pregnant females have two corpora lutea (CLs). The function of the accessory CL (ACL) is unknown; moreover, the process of ACL formation is unclear, and understanding this is necessary to know its role. To elucidate the process of ACL formation, the ovarian dynamics of six adult Hokkaido sika deer females were examined ultrasonographically together with peripheral estradiol-17β and progesterone concentrations. ACLs formed in three females that conceived at the first estrus of the breeding season, but not in those females that conceived at the second estrus. After copulation, postconception ovulation of the dominant follicle of the first wave is induced by an increase in estradiol-17β, which leads to formation of an ACL. A relatively low concentration of progesterone after the first estrus of the breeding season is considered to be responsible for the increase in estradiol-17β after copulation.  相似文献   

Estimating aboveground carbon using airborne LiDAR in Cambodian tropical seasonal forests for REDD+ implementation     

Tetsuji Ota  Tsuyoshi Kajisa  Nobuya Mizoue  Shigejiro Yoshida  Gen Takao  Yasumasa Hirata  Naoyuki Furuya  Takio Sano  Raul Ponce-Hernandez  Oumer S. Ahmed  Heng Sokh  Vuthy Ma  Eriko Ito  Jumpei Toriyama  Yukako Monda  Hideki Saito  Yoshiyuki Kiyono  Sophal Chann  Nang Ket 《Journal of Forest Research》2015,20(6):484-492

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Evaluation of larval growth process and bamboo consumption of the bamboo powder-post beetle Dinoderus minutus using X-ray computed tomography     

Hiroki Watanabe  Yoshiyuki Yanase  Yoshihisa Fujii 《Journal of Wood Science》2015,61(2):171-177

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Papaya seed represents a rich source of biologically active isothiocyanate   总被引：1，自引：0，他引：1  

Nakamura Y  Yoshimoto M  Murata Y  Shimoishi Y  Asai Y  Park EY  Sato K  Nakamura Y 《Journal of agricultural and food chemistry》2007,55(11):4407-4413

In the present study, papaya (Carica papaya) seed and edible pulp were carefully separated and then the contents of benzyl isothiocyanate and the corresponding glucosinolate (benzyl glucosinolate, glucotropaeolin) quantified in each part. The papaya seed with myrosinase inactivation contained >1 mmol of benzyl glucosinolate in 100 g of fresh seed. This content is equivalent to that of Karami daikon (the hottest Japanese white radish) or that of cress. The papaya seed extract also showed a very high activity of myrosinase and, without myrosinase inactivation, produced 460 micromol of benzyl isothiocyanate in 100 g of seed. In contrast, papaya pulp contained an undetectable amount of benzyl glucosinolate and showed no significant myrosinase activity. The n-hexane extract of the papaya seed homogenate was highly effective in inhibiting superoxide generation and apoptosis induction in HL-60 cells, the activities of which are comparable to those of authentic benzyl isothiocyanate.  相似文献   

Prematurational Culture with 3-Isobutyl-1-methylxanthine Synchronizes Meiotic Progression of the Germinal Vesicle Stage and Improves Nuclear Maturation and Embryonic Development in In Vitro-grown Bovine Oocytes     

Weiping HUANG  Masashi NAGANO  Sung-Sik KANG  Yojiro YANAGAWA  Yoshiyuki TAKAHASHI 《The Journal of reproduction and development》2014,60(1):9-13

The objective of this study was to clarify the effects of prematurational culture (pre-IVM) supplemented with 3-isobutyl-1-methylxanthine (IBMX) on nuclear and cytoplasmic maturation of in vitro-grown bovine oocytes. In experiment 1, oocytes (95 μm in diameter) derived from early antral follicles (0.5–1 mm in diameter) were cultured for 12 days for in vitro growth (IVG). IVG oocytes with a normal appearance were subjected to examinations of diameter and chromatin structure in the germinal vesicle (GV) before IVM. In addition, percentages of metaphase II (M II) were examined after IVM. Regardless of pre-IVM, the mean diameters of IVG oocytes were about 115 μm. The proportions of GV3 (50.0%) and M II stages (80.1%) of IVG oocytes with pre-IVM were higher than those without pre-IVM (28.0 and 49.4%, respectively). In experiment 2, the fertilizability and developmental competence of IVG oocytes were examined. Regardless of pre-IVM, the normal fertilization rates of IVG oocytes were similar (around 70%) but were lower than that of in vivo-grown oocytes (88.0%). Cleavage and blastocyst rates of IVG oocytes with pre-IVM (63.0 and 26.1%, respectively) were higher than those without pre-IVM (45.8 and 12.7%, respectively). The blastocyst rate based on cleaved IVG oocytes with pre-IVM (41.7%) was similar to that of in vivo-grown oocytes (48.7%), although the cleavage rate of IVG oocytes with pre-IVM was lower than that of in vivo-grown oocytes. In conclusion, pre-IVM with IBMX improved the maturational and developmental competences of IVG oocytes, probably due to promotion of their chromatin transition and synchronization of meiotic progression.  相似文献   

Estimation of the Optimal Timing of Fertilization for Embryo Development of In Vitro-Matured Bovine Oocytes Based on the Times of Nuclear Maturation and Sperm Penetration     

Keisuke KOYAMA  Sung-Sik KANG  Weiping HUANG  Yojiro YANAGAWA  Yoshiyuki TAKAHASHI  Masashi NAGANO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(5):653-659

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Functions of Chisan dams in large-scale granular mass-flow experiments on Sakurajima volcano pumiceous clasts     

Yasuhiko Okada 《Journal of Forest Research》2014,19(6):493-500

We investigated the fundamental behaviour of Chisan (check) dams in response to the presence or absence of impounded sediment with different levels of saturation. Large-scale model flume experiments were conducted by use of a model Chisan dam that had been backfilled with pumiceous clasts taken from the Sakurajima volcano in Kagoshima prefecture, Japan. In these experiments, the miniature dam was situated midway down the flume under different backfill sediment conditions and the basal pore-fluid pressure, normal flow depth, and impact load of the granular mass were carefully monitored. Saturated sediment with a volume of 0.6 m³ was released along the slope of the flume segment at 30° and excess pore-fluid pressure was generated, corresponding to a sediment velocity of approximately 4.8 m s^?1. Direct collision of the sediment with the Chisan dam meant the peak impact loads of granular mass against the back surface of the dam exceeded 157 N in experiments without backfill sediment but were as low as 32.1 N in experiments with sediment. Accordingly, placing the backfill sediment substantially reduced peak impact loads. The sediment captured by the Chisan dams was also calculated. For experiments using saturated backfill sediment, the dam captured only 35 % of the material, indicating that most of the granular mass passed over the dam. However, in experiments without backfill sediment or with unsaturated backfill sediment, over 90 % was captured. Although Chisan dams with unsaturated backfill sediment lacked pocket space, almost equivalent granular masses were trapped in these experiments. On the basis of these results, we suggest that Chisan dams with unsaturated backfill sediment could effectively counter debris flow, reducing sediment loads along the streams draining Sakurajima volcano.  相似文献   

Effect of dietary astaxanthin rich yeast,Phaffia rhodozyma,on meat quality of broiler chickens     

Ganzaya Perenlei  Hitomi Tojo  Toru Okada  Masatoshi Kubota  Motoni Kadowaki  Shinobu Fujimura 《Animal Science Journal》2014,85(10):895-903

We evaluated effects of dietary supplementation with astaxanthin (Ax)‐rich yeast, Phaffia rhodozyma (Xanthophyllomyces dendrorhous), on broiler chicken meat quality. Fourteen‐day‐old female Ross broilers were divided into three groups: control group, Ax‐free diet; Ax 10 group, 10 mg/kg Ax diet; and Ax 20 group, 20 mg/kg Ax diet for 28 days. At 42 days old, chickens were slaughtered, and then growth performance, meat quality and sensory attributes were analyzed. Compared with the control, a* values increased significantly after slaughter and 48 h postmortem for Ax 20 samples (P < 0.05) and for b* values in Ax 20 and Ax 10 groups (P < 0.05). Cooking loss decreased in the Ax 20 group (P < 0.05). After 120 h aging, contents of several free amino acids and total free amino acid content of Ax 20 group were significantly higher than the control (P < 0.05). In sensory evaluation, meat texture attributes improved significantly in the Ax 20 group (P < 0.01). No significant changes occurred in flavor attribute scores of meat soup from the Ax 20 group compared with the control even though most assessors preferred meat soup from the Ax 20 group. Overall, Ax‐rich yeast in the diet improves broiler chicken meat quality.  相似文献   

Spatial-uniform application method of methane fermentation digested slurry with irrigation water in the rice paddy field     

Kozue Yuge  Hiroki Maeda  Munehiro Tanaka  Mitsumasa Anan  Yoshiyuki Shinogi 《Paddy and Water Environment》2014,12(3):335-342

Promoting biomass utilization, the objectives of this study were to clarify the spatial distribution of nitrogen, one of the most important fertilizer components in the methane fermentation digested slurry (i.e., the digested slurry), and to establish an effective method to apply spatial-uniformly digested slurry with irrigation water in the rice paddy field. A numerical model describing the unsteady two-dimensional flow and solution transport of paddy irrigation water was introduced. The accuracy of this model was verified with a field observation. The tendencies of the TN simulated in inlet and outlet portions had good agreement with the measured data and the accuracy of the numerical model could be verified. Using the numerical model, scenario analyses were conducted to determine the method for spatial-uniform application of the digested slurry with irrigation water. The simulated results indicated that drainage of the surface water and trenches at the soil surface were effective for spatial-uniform application of the digested slurry with irrigation water in the rice paddy fields. The effect of the trenches was maximized when the surface water of the rice paddy field was drained adequately.  相似文献   

用单抗研究T细胞与牛流行性白血病发生的关系     

潘耀谦  夏志平  高丰  冈田幸助 《动物医学进展》2001,22(4):75-78

本试验对10头患流行性白血病病牛的不同器官的肿瘤组织，用8种单克隆抗体（McAb),TH14B、BAQ44A、PIg45A、BIg715A、PIg501E、cAct105、MM1A、AHCC125染色进行了观察。结果：病牛经临床病理学和免疫琼扩试验而诊断为EBL，通过免疫组织化学检测，在10个病例中发现有9个病例的肿瘤组织对B细胞属性的McAb有很强的染色反应，证明其来源于B细胞。仅有1个病例对B细胞属性的McAb着色很淡，而对T细胞属性的McAb着色很深，呈强阳性反应，说明其来源可能与T细胞有关。  相似文献