Changes of lymphocyte subpopulations and natural killer cells in mice sensitized with Toxoplasma lysate antigen before and after Babesia infection     

I Igarashi  R Honda  T Shimada  K Miyahara  H Sakurai  A Saito  N Suzuki 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(5):969-977

When 8-week-old BALB/c mice were sensitized with two intramuscular injections of Toxoplasma lysate antigen (TLA) at 2 week interval, the numbers of sIg(+), Thy-1,2(+), Lyt-1,2(+) Lyt-2,2(+), and Asialo GM1(ASGM1)(+) cells in the spleen, liver and peripheral blood increased by 2 to 4 times over those found in unsensitized mice of the same age. When TLA-sensitized and unsensitized mice were infected with Babesia, 4 of 10 (40%) of the TLA-sensitized mice survived infection, while none of the unsensitized control mice lived longer than 14 days after Babesia infection. By contrast, sensitization of nude mice with TLA had no effect on survival, and mice did not live more than 12 days. The number of thymic Thy-1,2(+) cells decreased in TLA-sensitized and unsensitized BALB/c mice by almost 80% within 10 days after infection (AI). During the same time, the numbers of B cells, T cells, and NK cells increased in the spleen, liver and peripheral blood of both sensitized and unsensitized mice. Especially notable were increases in numbers of Lyt-2,2(+) cells in the spleen and blood and increases in numbers of NK cells in the spleen, liver and blood in both TLA-sensitized and unsensitized mice. When spleen cells from TLA-sensitized and unsensitized mice were cultured in the presence or absence of TLA for 6 days, assays for cytotoxicity using NK-insensitive P-815 target cells and NK-sensitive YAC-1 target cells demonstrated higher rates of cytotoxicity in cultures of TLA-sensitized spleen cells.  相似文献   

A serological comparison of 4 Japanese isolates of porcine enteroviruses with the international reference strains   总被引：2，自引：0，他引：2  

E Honda  A Kimata  I Hattori  T Kumagai  T Tsuda  T Tokui 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(1):49-54

The reference strains of four serotypes (J6, J8, J9 and J10) of porcine enterovirus isolated in Japan were compared with the international reference strains of 11 serotypes (W1 to W11) by cross neutralization tests. No cross reactions were observed between the two groups, although there were minor one-way crosses between W10 and J10, W11 and J9, and W4 and J9. This leads to our conclusion that all 4 Japanese serotypes can be newly added to 11 international serotypes. J9 virus produced type 1 of CPE (CPE I), J6 and J8 did CPE II, and J10 did CPE III. J10 grew in Vero, HeLa cells, and the primate cells, but J6, J8 and J9 did not.  相似文献   

Appearance of pH-dependent cell fusions by transmissible gastroenteritis (TGE) virus     

E Honda  H Takahashi  K Okazaki  T Kumagai 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(4):839-841

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A network of superconducting gravimeters detects submicrogal coseismic gravity changes   总被引：8，自引：0，他引：8  

Imanishi Y  Sato T  Higashi T  Sun W  Okubo S 《Science (New York, N.Y.)》2004,306(5695):476-478

With high-resolution continuous gravity recordings from a regional network of superconducting gravimeters, we have detected permanent changes in gravity acceleration associated with a recent large earthquake. Detected changes in gravity acceleration are smaller than 10(-8) meters seconds(-2) (1 micro-Galileo, about 10(-9) times the surface gravity acceleration) and agree with theoretical values calculated from a dislocation model. Superconducting gravimetry can contribute to the studies of secular gravity changes associated with tectonic processes.  相似文献   

Subaru telescope observations of Deep Impact     

Sugita S  Ootsubo T  Kadono T  Honda M  Sako S  Miyata T  Sakon I  Yamashita T  Kawakita H  Fujiwara H  Fujiyoshi T  Takato N  Fuse T  Watanabe J  Furusho R  Hasegawa S  Kasuga T  Sekiguchi T  Kinoshita D  Meech KJ  Wooden DH  Ip WH  A'Hearn MF 《Science (New York, N.Y.)》2005,310(5746):274-278

The impact cratering process on a comet is controversial but holds the key for interpreting observations of the Deep Impact collision with comet 9P/Tempel 1. Mid-infrared data from the Cooled Mid-Infrared Camera and Spectrometer (COMICS) of the Subaru Telescope indicate that the large-scale dust plume ejected by the impact contained a large mass (approximately 10(6) kilograms) of dust and formed two wings approximately +/-45 degrees from the symmetric center, both consistent with gravity as the primary control on the impact and its immediate aftermath. The dust distribution in the inner part of the plume, however, is inconsistent with a pure gravity control and implies that evaporation and expansion of volatiles accelerated dust.  相似文献   

Regulation of spermatogenesis by testis-specific,cytoplasmic poly(A) polymerase TPAP     

Kashiwabara S  Noguchi J  Zhuang T  Ohmura K  Honda A  Sugiura S  Miyamoto K  Takahashi S  Inoue K  Ogura A  Baba T 《Science (New York, N.Y.)》2002,298(5600):1999-2002

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Co-transmission of <Emphasis Type="Italic">Tomato yellow leaf curl virus</Emphasis> (TYLCV)-Mld and TYLCV-IL by the whitefly <Emphasis Type="Italic">Bemisia tabaci</Emphasis>     

Jun Ohnishi  Toshio Kitamura  Fumihiro Terami  Ken-ichiro Honda 《Journal of General Plant Pathology》2011,77(1):54-59

The ability of the whitefly Bemisia tabaci to transmit two strains of Tomato yellow leaf curl virus, the Israel and Mild strains, was studied after serial transfers of individual whiteflies that were viruliferous for both strains to tomato plants. After single whiteflies had successive acquisition feedings first on a single plant infected with one strain and then on a plant infected with the other strain, the single whiteflies later transmitted intermittently one, the other, or both strains to the test plants during serial transfers at 1-day intervals. Because both strains were found in the head, abdomen, and legs dissected from whiteflies during the retention period after the two successive acquisition feedings, both strains apparently circulate from midgut cells to salivary glands through the hemolymph.  相似文献   

Analysis of properties of monoclonal antibodies to transmissible gastroenteritis virus using TO-163 strain     

E Honda  H Kanemaru  X Y Dong  M Ikuma  Y Somura  I Shibata  K Okazaki  T Kumagai 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1990,37(9):660-667

Nineteen monoclonal antibodies (MAbs), reactive in enzyme-linked immunosorbent assay (ELISA), with porcine transmissible gastroenteritis (TGE) virus TO-163 were obtained. Of these MAbs, 5 showed neutralizing (NT) activity (x 3,200 to 25,600) against TO-163. One of the MAbs which had NT activity showed hemagglutination inhibition activity (x 5,120) too. 14 hybridomas of polypeptide specificity against TO-163 strain were developed from which 11, 2, and 1 were specific for protein E2, N, and E1, respectively. Immunofluorescence staining patterns in TGE virus-infected cells reacted with MAbs were divided into three groups (types I, II and III). The fluorescence staining of E2 specific MAbs having NT activity were limited to the perinuclear area. All MAbs having NT activity showed the same fluorescence staining pattern.  相似文献   

Detection of bovine rhinotracheitis virus antibody by neutralizing test and ELISA in experimentally infected rabbits.     

E Honda  T Taniguchi  M Watanabe  T Kumagai 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1991,38(1):55-59

The antibody response of rabbit to infectious bovine rhinotracheitis (IBR) virus was examined. Two rabbits were inoculated with IBR virus strain Los Angeles into the trachea and intravenously, and intravenously two times, respectively. The patterns of antibody titers in the rabbits measured by neutralization test and ELISA were similar to those in the case of bovine. The antibody was detected after the inoculation, and much more antibody was detected after the second inoculation. The fact suggests that rabbits are a very useful laboratory animal for IBR virus infection studies.  相似文献   

Safety and efficacy of the cell-associated vaccine prepared by an attenuated infectious laryngotracheitis virus.     

A Taneno  T Honda  E Sakai  T Kawai  Y Tokuyama  M Eto 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(4):671-676

The safety and efficacy of the cell-associated (C-A) vaccine prepared by chicken embryo fibroblast (CEF) cells infected with the tissue-culture-modified strain of infectious laryngotracheitis (ILT) virus were studied in chickens. Over seventy percent of chickens inoculated with the C-A vaccine by the subcutaneous (S.C.) or intramuscular (I.M.) route at 1 day of age was protected against challenge with a virulent strain of ILT virus without any clinical signs. Chickens vaccinated with the C-A vaccine at 1 day of age acquired immunity within 6 days after vaccination, and the protection rate maintained more than 60% until 10 weeks post-vaccination. The C-A vaccine was invariably effective for chickens at various age. There was no evidence that the development of immunity was hindered by further vaccination with Newcastle disease and infectious bronchitis combined live vaccine. In addition, the C-A vaccine was safe when chickens were inoculated with 10 doses. In the field trials of the C-A vaccine, no adverse reaction was observed, and over 65% of vaccinated chickens was protected against the challenge of the virulent ILT virus at 8 weeks after vaccination.  相似文献