Contraction of the microfibrils of wood treated with aqueous NaOH: evidence from changes in the anisotropy of the longitudinal and transverse swelling rates of wood     

Yukiko Ishikura  Takato Nakano 《Journal of Wood Science》2007,53(2):175-177

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Physicochemical properties of pseudorabies virus hemagglutinin.     

J Kataoka  Y Inaba  N Tetsu  I Shibata  K Yoshiki  T Hirahara  A Izumida 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(6):981-987

Pseudorabies virus hemagglutinin was readily adsorbed on mouse erythrocytes at 4, 22, or 37 degrees C, but not on cattle erythrocytes. The adsorbed hemagglutinin could not be eluted from the cells by resuspending in phosphate-buffered saline (PBS), by incubating at 37 or 50 degrees C, or by incubating in the presence of neuraminidase. The receptor on mouse erythrocytes for the hemagglutinin was inactivated by trypsin, but not by neuraminidase, sodium deoxycholate (DOC), potassium periodate (KIO4), dithiothreitol (DTT), 2-mercaptoethanol (2-ME) and formalin. The hemagglutinin was inactivated by trypsin, alpha-amylase, pepsin, DOC, KIO4, and ethylendiamine-tetraacetic acid (EDTA), but not by papain, beta-glucosidase, phospholipase C, neuraminidase, DTT, 2-ME, Tween-80, ethylether, chloroform, trichloro-trifluoroethane, beta-propiolactone and formalin, suggesting that the hemagglutinin active component involved glycoproteins. The hemagglutinin was stable at 37 degrees C for lower temperatures but not at 60 degrees C or higher. The hemagglutinin activity was resistant to ultraviolet irradiation, while the infectivity was very susceptible. The hemagglutinin and the infectivity were readily sedimented by ultracentrifugation at 48,000 x g for 3 hr. In rate zonal centrifugation of the preparation on a sucrose density gradient, the hemagglutination (HA) activity showed a sharp peak at 1.22 g/ml coinciding with the peak of infectivity. The HA activity in the peak fraction seemed to be structually associated with virus particles. After fractionation of the virus by Nonidet P-40, the HA activity was found only in the fraction of the envelope material, indicating that the hemagglutinin is situated in the viral envelop.  相似文献   

High performance liquid chromatographic determination of trimethoprim residues in egg yolk and albumen in a feeding experiment     

T Nagata  M Saeki  T Iida  M Kataoka  S Shikano 《The British veterinary journal》1991,147(4):346-351

In a feeding experiment, the residues of trimethoprim (TMP) in egg yolk and albumen were determined by high performance liquid chromatography (HPLC). Laying hens were divided into four groups. The first group was designated as control and fed with TMP free diet during the experimental period. The second, third and fourth groups were administered with the feeds containing 4 p.p.m., 16 p.p.m. and 56 p.p.m. TMP, respectively, for 19 days and, thereafter, fed with TMP free diet. TMP was mainly found in yolk in all the three administered levels. In the medication period, the average concentration of TMP in the yolk of the second, third and fourth groups was 0.05, 0.25, and 0.90 p.p.m., respectively. After withdrawal of medication, the residues of TMP in yolk in the second, third and fourth groups decreased below the detection limit at 0.02 p.p.m. on days 4, 9, and 11, respectively.  相似文献   

Establishment of a novel feline leukemia virus (FeLV)-negative B-cell cell line from a cat with B-cell lymphoma     

Mochizuki H  Takahashi M  Nishigaki K  Ide T  Goto-Koshino Y  Watanabe S  Sato H  Sato M  Kotera Y  Fujino Y  Ohno K  Uchida K  Tsujimoto H 《Veterinary immunology and immunopathology》2011,141(3-4):307-311

There is significant lack of basic hematologic and immunological data in adult sows. Therefore, aim of this study was to provide respective reference intervals. 32 clinically healthy multiparous Large White sows aged 33.5 ± 9.6 months and all of them two months postpartum were included in this study. Mean erythrocyte count was 5.5 ± 0.7 × 10(6)/μl and total leukocyte count was 12.1 ± 2.1 × 10(3)/μl. Proportion of lymphocytes was 44.7 ± 10.2% and of neutrophils 41.6 ± 11.0%. The ratio of na?ve T helper (Th) cells to memory Th cells was 1:3.1 and the ratio of Th cells to cytotoxic T cells (CTLs) was 1:4.2. Proportions of regulatory T cells, NK cells, and CD21(+) B cells were lower (3.1, 2.6, and 6.0%) than those of memory Th cells ranging from 8.8 to 27.5% depending on the activation status and CTLs with 37.3%. γδ T cells were found at comparably high numbers (19.1%). Flow cytometric measurement of intracellular cytokines in PBMCs revealed marginal levels for IL-1β, IL-2, IL-4, IL-6, IL-10, and IL-12p35, but remarkable levels for TNF-α and IFN-γ. Highest mRNA levels were found for IL-1, IL-10, and TNF-α, with TNF-α showing the least inter-individual variation.  相似文献   

Survival of Salmonella strains differing in their biofilm-formation capability upon exposure to hydrochloric and acetic acid and to high salt     

Hasegawa A  Hara-Kudo Y  Kumagai S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1163-1168

Acidic and osmotic treatments are part of hurdle systems to control pathogens such as Salmonella in food. In the current study, Salmonella enterica isolates previously shown to differ in their ability to form biofilms were grown in diluted tryptic soy broth (TSB) (1:5 dilution in distilled water) and subsequently exposed to phosphate-buffered saline (PBS) adjusted to pH 3.0 with HCl, PBS adjusted to pH 3.9 with acetic acid or rice vinegar diluted 1:15 with distilled water (pH 3.9). Cells grown in diluted TSB were also exposed to distilled water, pH 7.6, containing 5 M NaCl. No differences in survival upon exposure to PBS adjusted to pH 3.0 with HCl or distilled water containing high salt were observed between the isolates; however, exposure to acetic acid and rice vinegar resulted in lower survival levels of isolates previously shown to be poor biofilm formers. The numbers (log(10) cfu/ml) of surviving cells after exposure for 36 hr to acetic acid and rice vinegar were 4.43 ± 0.24 vs. 2.27 ± 0.87 (P<0.05) and 5.19 ± 0.12 vs. 2.33 ± 0.93 (P<0.05) for isolates with a high vs. low biofilm-forming ability. The survival data could be fitted with the Weibull model. The data suggest that the ability of Salmonella strains to survive in the presence of acetic acid and rice vinegar parallels their ability to form biofilms. Thus, Salmonella with a high biofilm-formation capability might be more difficult to kill with acetic acid found in foods or cleaning solutions.  相似文献   

Surveillance of avian influenza virus in migratory water birds in eastern Hokkaido, Japan     

Bui VN  Ogawa H  Karibe K  Matsuo K  Nguyen TH  Awad SS  Minoungou GL  Xininigen  Saito K  Watanabe Y  Runstadler JA  Happ GM  Imai K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(2):209-215

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Strawberry cultivar identification based on hypervariable SSR markers     

Masanori Honjo  Tsukasa Nunome  Sono Kataoka  Takayoshi Yano  Hiromichi Yamazaki  Megumi Hamano  Susumu Yui  Masami Morishita 《Breeding Science》2011,61(4):420-425

We genotyped strawberry cultivars by two newly selected and two previously reported SSR markers. All four markers produced interpretable electropherograms from 75 accessions consisting of 72 Fragaria × ananassa cultivars or lines and three octoploid Fragaria species accessions. These SSR markers were highly polymorphic; in particular, one of the newly developed markers, FxaHGA02P13, was capable of distinguishing all of the accessions except for a mutant strain that was derived from another accession in the set. When two markers were combined, all 48 full-sib individuals could be distinguished. Fingerprinting patterns were reproducible between multiple samples, including the leaves, sepals, and fruit flesh of the same accession. Principal-coordinate analysis of the 75 accessions detected several groups, which reflect taxon and breeding site. Together with other available markers, these SSR markers will contribute to the management of strawberry genetic resources and the protection of breeders’ rights.  相似文献   

The biological activities of cardenolide triglycosides from stems, twigs, and leaves of Nerium oleander     

Yuhua Bai  Ming Zhao  Liming Bai  Ryo Hasegawa  Jun-ichi Sakai  Toshiaki Hasegawa  Tomokazu Mitsui  Hirotsugu Ogura  Takao Kataoka  Katsutoshi Hirose  Masayoshi Ando 《Journal of Wood Science》2011,57(1):56-65

Sixteen cardenolide triglycosides (1?C16) were isolated from stems, twigs, and leaves of Nerium oleander. Among them, 3??-O-(4-O-gentiobiosyl-d-diginosyl)-7??,8-epoxy-14-hydroxy-5??,14??-card-20(22)-enolide, named cardenolide B-3 (16), was isolated from natural sources for the first time. The in vitro anti-inflammatory activities of compounds 1?C16 were examined on the basis of inhibitory activity against the induction of intercellular adhesion molecule-1 (ICAM-1). Compounds 1?C5 were active at an IC50 value of less than 7 ??M. The cytotoxic activity of isolated compounds was evaluated against three human cell lines: normal human fibroblast cells (W-38), malignant tumor cells induced from WI-38 (VA-13), and human liver tumor cells (HepG2). Compounds 1?C5 were active toward WI-38 cells; compounds 1, 3, and 5 were active toward VA-13 cells; and compounds 1?C5 were active toward HepG2 cells at IC₅₀ values of less than 10 ??M. The multidrug-resistant (MDR) cancer-reversal activity of compounds 1?C16 was evaluated on the basis of the amount of calcein accumulated in MDR human ovarian cancer 2780AD cells in the presence of each compound. Compounds 13 and 14 showed significant effects on calcein accumulation.  相似文献   

Cell death process initiated by a combination of aluminium and iron in suspension-cultured tobacco cells (Nicotiana tabacum): Apoptosis-like cell death mediated by calcium and proteinase     

Yukiko Yamaguchi  Yoko Yamamoto  Hideaki Matsumoto 《Soil Science and Plant Nutrition》2013,59(3):647-657

Aluminium (AI) enhances ferrous ion (Fe²⁺) -mediated lipid per oxidation which is the primary factor leading to cell death in nutrient medium in tobacco cells. Under these conditions, cell death processes were examined. Cells which had been treated with AI and Fe²⁺ together exhibited cell shrinkage and DNA ladders which were detected by agarose-gel electrophoresis in the form of bands corresponding to increasing multiples of ～150 bp fragments. In the calcium (Ca)-free medium, a combination of Al and Fe2+ enhanced only partly the per oxidation of lipids and hardly promoted cell death, indicating that Ca²⁺ further stimulates the per oxidation of lipids enhanced by a combination of Al and Fe²⁺ and is required for cell death. N-Tosyl-L-phenylalanylchloromethyl ketone, an inhibitor of some cysteine and serine proteinases, had no effect on the per oxidation of lipids but almost completely blocked cell death enhanced by a combination of AI and Fe²⁺, suggesting that proteinase acts downstream from the per oxidation of lipids. On the basis of these findings, we conclude that cell death initiated by a combination of Al and Fe²⁺ is involved in the apoptosislike cell death program mediated by extracellular Ca²⁺ and endogenous proteinase.  相似文献   

Morphology of placentome in Korean water deer Hydropotes inermis argropus     

Joon Hyuk SOHN  Shota YAMANE  Yukiko SAITOH  Ken Takashi KUSAKABE  Junpei KIMURA  Yasuo KISO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(7):1081

The placenta of the Korean water deer was anatomically examined to accumulate basic information regarding its reproductive system. The convex placentomes with five to nine well-developed pedicles were observed in the whole uterine horns, and therefore, the placenta was classified as oligocotyledonary. The evidence indicating the migration of binucleate cells (BNCs) from trophectoderm to the uterine epithelium led to the histological classification of the placenta as synepitheliochorial. The number of fetuses was markedly higher than that in other ruminant species. However, the number of placentomes was found to be similar to the other Cervidae species. Therefore, these results suggest that the Korean water deer may possess special mechanisms or structures at the fetus attachment site to maintain this unusally high number of fetuses.  相似文献