全文获取类型
收费全文 | 221篇 |
免费 | 4篇 |
专业分类
林业 | 21篇 |
农学 | 7篇 |
基础科学 | 1篇 |
39篇 | |
综合类 | 13篇 |
农作物 | 2篇 |
水产渔业 | 11篇 |
畜牧兽医 | 111篇 |
园艺 | 8篇 |
植物保护 | 12篇 |
出版年
2023年 | 1篇 |
2022年 | 4篇 |
2021年 | 10篇 |
2020年 | 1篇 |
2018年 | 3篇 |
2017年 | 2篇 |
2016年 | 3篇 |
2015年 | 7篇 |
2014年 | 4篇 |
2013年 | 14篇 |
2012年 | 16篇 |
2011年 | 16篇 |
2010年 | 8篇 |
2009年 | 8篇 |
2008年 | 8篇 |
2007年 | 14篇 |
2006年 | 17篇 |
2005年 | 11篇 |
2004年 | 16篇 |
2003年 | 19篇 |
2002年 | 15篇 |
2001年 | 10篇 |
2000年 | 3篇 |
1999年 | 3篇 |
1994年 | 1篇 |
1992年 | 1篇 |
1991年 | 4篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1984年 | 1篇 |
1974年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有225条查询结果,搜索用时 15 毫秒
191.
A gas chromatographic-mass spectrometric method for the determination of S-methyl-L-cysteine sulfoxide (1), S-propyl-L-cysteine sulfoxide (2), and S-propenyl-L-cysteine sulfoxide (3), specific marker compounds in the genus Allium, is described. The target amino acids were converted to the tert-butyldimethylsilyl derivatives. The products were silylated on the amino and carboxyl groups and on an additional oxygen atom and were separated on a nonpolar capillary column. That incorporation of three tert-butyldimethylsilyl groups had occurred was verified by mass spectrometry, which gave an m/z 302 fragment as base peak (amino acid side chain eliminated ion) and m/z 436 (1), 464 (2), or 462 (3) as major peaks (tert-butyl function eliminated ion), by electron impact ionization. The detection limits for 1 and 2 under selected ion monitoring at m/z 436 (1) and m/z 464 (2), respectively, were determined to be 0.3 and 1.8 ng per injection. To clean up the analytes from the solvent extract of onion, as a representative food material, onion, the sample solution was subjected to combined solid phase extraction. The eluate from a Sep-Pak C(18) cartridge was applied to a Bond Elut SCX cartridge (H(+) form), followed by washing with 0.1 M hydrochloric acid and elution with 0.5 M ammonia. From a simulated matrix solution containing 5% sucrose, 1 and 2 were extracted quantitatively, and the detection yield was approximately 75%. The contents of 1, 2, and 3 in commercial onion were estimated to be 0.3, 3.1, and 3.0 mg, respectively, per gram of fresh weight. 相似文献
192.
Effects of food materials were investigated on removal of several kinds of thiols, sulfides, and disulfides, which arise from vegetables of Allium species during food preparation and eating. Methanethiol, propanethiol, and 2-propenethiol were captured by raw foods such as fruits, vegetables, and mushrooms or a mixture of their acetone powders and phenolic compounds. The odor of diallyl disulfide was remarkably reduced by kiwi fruit, spinach, cutting lettuce, parsley, basil, mushrooms, and, particularly, cow's milk, raw egg, boiled rice, and bovine serum albumin (BSA). This suggests that the removal of diallyl disulfide could be caused by a physical and chemical interaction between the disulfide and foods. Furthermore, milk and BSA captured propanethiol, 2-propenethiol, dipropyl sulfide, diallyl sulfide, dimethyl disulfide, and dipropyl disulfide very well. An enzymatic degradation of diallyl disulfide by spinach and asparagus was also observed. These results demonstrate that the deodorization with foods is achieved by multiple actions including physical and chemical interaction between volatile sulfur compounds and foods, enzymatic degradation of disulfides, and addition of thiols to polyphenolic compounds, catalyzed by polyphenol oxidases or peroxidases. 相似文献
193.
Sako T Mori A Lee P Igarashi A Oda H Saeki K Miki Y Kurishima M Mimura K Nozawa S Itabashi Y Kiyosawa Y Ishioka K Arai T 《Veterinary research communications》2011,35(4):201-209
The purpose of this study was to investigate and propose possible reference intervals of plasma biochemical analytes in young dogs (<12 months old) in Japan, using 896 canine plasma samples, collected from an array of veterinary clinics throughout the greater Tokyo metropolis area in Japan. The following biochemical parameters were assessed: albumin (ALB), alanine aminotransferase (ALT), alkaline phosphatase (ALP), amylase, aspartate aminotransferase (AST), blood urea nitrogen (BUN), creatinine (CRE), glucose, lipase, total cholesterol (T-Cho), and total protein (TP) were evaluated. Multivariate linear regression analysis indicated that partitioning according to age or gender may be necessary for some plasma analytes. Age appeared to significantly affect ALB, ALT, ALP, BUN, Glucose, Lipase, and Total Protein (P=?<0.001, <0.001, <0.001, 0.013, <0.001, 0.025, and <0.001, respectively). On the other hand, gender significantly influenced ALP, Amylase, Lipase, and T-Cho levels (P=0.017, <0.001, <0.001, and <0.001, respectively) whereas it may be borderline significant with ALT (P=0.072). 相似文献
194.
The suppressive effect of dietary coenzyme Q10 on mitochondrial reactive oxygen species production and oxidative stress in chickens exposed to heat stress
下载免费PDF全文
![点击此处可从《Animal Science Journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Motoi Kikusato Kasumi Nakamura Yukiko Mikami Ahmad Mujahid Masaaki Toyomizu 《Animal Science Journal》2016,87(10):1244-1251
This study was conducted to determine if dietary supplementation with coenzyme Q10 (CoQ10), which can act as a potent antioxidant and is an obligatory cofactor of mitochondrial uncoupling protein, suppresses the heat stress (HS)‐induced overproduction of mitochondrial reactive oxygen species (ROS) and oxidative damage in the skeletal muscle of birds. The carbonyl protein content of skeletal muscle was significantly higher in birds exposed to HS treatment (34°C, 12 h) than in thermoneutral birds (25°C). This increase was suppressed by CoQ10 supplementation (40 mg/kg diet). Succinate‐supported mitochondrial ROS production was increased by HS treatment, and this increase was also suppressed by CoQ10 supplementation. In contrast, CoQ10 supplementation did not affect the HS‐induced decrease in mitochondrial proton leak. The mitochondrial membrane potential (ΔΨ), to which HS‐induced ROS production was previously shown to be sensitive, tended to be increased by HS treatment, but this rise in ΔΨ was not affected by CoQ10 supplementation. Taken together, these results suggest that dietary CoQ10 supplementation attenuates HS‐induced oxidative damage to skeletal muscle, by preventing the overproduction of succinate‐supported mitochondrial ROS in a manner that is independent of ΔΨ. © 2015 Japanese Society of Animal Science 相似文献
195.
Yukiko Sakata Bekku Hiroyuki Kurokochi Yu Matsuki Engkong Tan Shuichi Asakawa Satoshi Imura Chunlan Lian 《Journal of Forest Research》2016,21(4):193-196
Pinus parviflora Sieb. et Zucc. var. parviflora is a coniferous tree species distributed in mountainous areas of temperate zones from southern Tohoku to the Kyushu area in Japan. Some P. parviflora populations have become small and endangered due to massive death resulting from pine wilt disease, scab canker, and presumably climate change. We developed 11 microsatellite markers for P. parviflora by the dual-suppression technique and next-generation sequencing using 32 individuals of P. parviflora collected from Aokigahara at the foot of Mt. Fuji. The number of alleles for each locus ranged from two to 10. The averages of observed and expected heterozygosities were 0.61 and 0.59, respectively. These markers will become powerful tools for assessing genetic diversity, genetic connectivity, and genetic structure in P. parviflora populations, which will facilitate our understanding and conservation of P. parviflora. 相似文献
196.
Required sample size for estimating soil respiration rates in large areas of two tropical forests and of two types of plantation in Malaysia 总被引:1,自引:0,他引:1
Minako Adachi Yukiko Sakata Bekku Akihiro Konuma Wan Rasidah Kadir Toshinori Okuda Hiroshi Koizumi 《Forest Ecology and Management》2005,210(1-3):455-459
We estimated the required sample sizes for estimating large-scale soil respiration (for areas from 1 to 2 ha) in four ecosystems (primary and secondary forests, and oil palm and rubber plantations) in Malaysia. The soil respiration rates were 769 ± 329 mg CO2 m−2 h−1 in the primary forest (2 ha, 50 sample points), 708 ± 300 mg CO2 m−2 h−1 in the secondary forest (2 ha, 50 points), 815 ± 363 mg CO2 m−2 h−1 in the oil palm plantation (1 ha, 25 points), and 450 ± 178 mg CO2 m−2 h−1 in the rubber plantation (1 ha, 25 points). According to our sample size analysis, the number of measurement points required to determine the mean soil respiration rate at each site with an error in the mean of no more than 10% ranged from 67 to 85 at the 95% probability level. These results suggest that evaluating the spatial heterogeneity of soil respiration rates in the tropics may require more measurement points than in temperate forests. 相似文献
197.
Eiji YAMAMOTO Hiroshi ITO Yukiko TOMIOKA Toshihiro ITO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1079-1085
An avian paramyxovirus (APMV) isolated from goose feces (APMV/Shimane67) was
biologically, serologically and genetically characterized. APMV/Shimane67 showed typical
paramyxovirus morphology on electron microscopy. On hemagglutination inhibition test,
antiserum against APMV/Shimane67 revealed low reactivity with other APMV serotypes and
vice versa. The fusion (F) protein gene of APMV/Shimane67 contained
1,638 nucleotides in a single open reading frame encoding a protein of 545 amino acids.
The cleavage site of F protein contained a pair of single basic amino
acid (VRENR/L). The nucleotide and deduced amino acid sequences of the F gene
of APMV/Shimane67 had relatively low identities (42.9–62.7% and 28.9–67.3%, respectively)
with those of other APMVs. Phylogenetic analysis showed that APMV/Shimane67 was related to
NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes. These results suggest
that APMV/Shimane67 is a new APMV serotype, APMV-13. 相似文献
198.
Fujii K Kakumoto C Kobayashi M Saito S Kariya T Watanabe Y Sakoda Y Kida H Suzuki M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(3):259-263
For proper management and conservation of the Kuril harbor seal (Phoca vitulina stejnegeri) through disease control, serological analysis was performed for influenza A virus infection in free-ranging seals in Hokkaido, Japan. Serum samples were collected from seals at Nosappu (231 seals), Akkeshi (16) and Erimo (75), between 1998 and 2005, and were analyzed by ELISA. Antibodies to the influenza A virus were detected only in seals from Nosappu. The incidences were 11% (1/9), 3% (2/66), 12% (7/59) and 6% (5/77) in 1998, 2003, 2004 and 2005, respectively. These suggest sporadic infection. Because antibody-positive seals included juvenile seals in each year, the infections were considered to have been circulated since no later than the late 1990s until recent years. ELISA-positive sera were analyzed by hemagglutination inhibition (HI) tests to determine the subtypes. Antibodies to the H3 and H6 subtypes were detected in 10 and 2 sera, respectively. Two of the sera that had antibodies to the H6 subtype also had antibodies to the H3 subtype. These two seals were considered to have been infected with both the H3 and H6 subtypes. This is the first investigation to find antibodies to the H6 subtype in seals. Although the H6 subtype had been isolated only from avians, genetic analysis had suggested that the H6 subtype could become a novel mammalian pathogen. For definitive diagnosis, detection of the virus from the tissue or mucus of seals is required. 相似文献
199.
200.
The effects of the addition of gibberellic acid (GA) to auxin solutions for tomato fruit setting on sugar content and titratable acidity at maturity were investigated in ‘Louis 60’ tomatoes, and sucrolytic enzyme activities and cell development during the early stages of fruit development were examined to explain the physiological mechanisms. Days to maturity and titratable acidity were not affected by GA addition. The sugar concentration of the pericarp was increased by 50 mg L−1 of GA addition in the summer and the spring, but not in the autumn experiment. The product of sugar concentration and fruit weight, which is thought to reflect the amount of sugar per fruit, was higher in GA-treated fruit in every experiment. Beginning 1 day after treatment, cell size was larger in GA-treated fruit and resulted in an increase in pericarp thickness at maturity. These results suggest that GA addition at anthesis can promote an increased sink size of individual pericarp cells immediately after treatment. The activities of VAI and neutral invertase (NI) were higher in GA-treated fruit around 5 days after treatment, and the possibility of VAI and/or NI involvement in sink activity at an early stage of fruit development is suggested. 相似文献