Complete mitochondrial DNA sequence of the Japanese flying fish <Emphasis Type="Italic">Cypselurus hiraii</Emphasis>   总被引：2，自引：0，他引：2  

Mitsutoshi?NagaseEmail author  Tadanori?Aimi  Katsuaki?Suginaka  Yutaka?Kitamoto  Tsutomu?Morinaga 《Fisheries Science》2005,71(4):914-923

ABSTRACT:   In this study, to develop a technique that enables authentication of processed seafood, the complete nucleotide sequence of the mitochondrial genome for the Japanese flying fish Cypselurus hiraii was determined. Three segments spanning the entire genome were amplified using polymerase chain reaction, and products were subsequently used as templates for direct sequencing with 60 primers. The genome (16 528 base pairs) was found to contain the same 37 genes (two ribosomal RNA, 22 transfer RNA, and 13 protein-coding genes) as those found in other vertebrate mitochondrial genomes, with the gene order being identical to that typical of vertebrates. A major non-coding region between the tRNA^Pro and tRNA^Phe genes (868 base pairs) appears to be the control (D-loop) region, as it has several conserved blocks characteristic of control regions.  相似文献   

Improvement of ovulation induction by additive injection of 17,20β‐Dihydroxy‐4‐pregnen‐3‐one after human chorionic gonadotropin administration in a pelagic egg spawning marine teleost,nibe croaker Nibea mitsukurii (Jordan & Snyder)          下载免费PDF全文

Yoji Yamamoto  Takashi Yatabe  Kentaro Higuchi  Yutaka Takeuchi  Goro Yoshizaki 《Aquaculture Research》2015,46(6):1323-1331

This study aimed to develop the consistent ovulation induction method in a pelagic egg spawning marine teleost, nibe croaker Nibea mitsukurii. Attempts to induce oocyte maturation and ovulation in nibe croaker using human chorionic gonadotropin (hCG; 0.5 IU g^?1) resulted in the normal progression of oocyte maturation and hydration, but a failure to induce ovulation in many individuals. This ovulation disorder was similarly observed even when the dose of hCG was increased 10 times (5 IU g^?1) or decreased to one tenth (0.05 IU g^?1), indicating that it cannot be completely overcome solely by hCG administration. However, this ovulation disorder could be completely overcome by subsequent administration of 17,20β‐dihydroxy‐4‐pregnen‐3‐one (DHP) at the appropriate dose (0.5 μg g^?1) and time (20 h after hCG administration). An increase in the number of individuals that ovulated due to DHP administration led to an increase in individuals producing larvae, resulting in an approximately threefold increase in the estimated number of larvae produced compared with the group of fish administered hCG alone. Thus, this ovulation induction method using DHP administration after hCG was demonstrated to overcome the ovulation disorder in nibe croaker and could be applicable to commercially important species with similar ovulation problems.  相似文献