Anthelmintic activity of Trianthema portulacastrum L. and Musa paradisiaca L. against gastrointestinal nematodes of sheep     

Hussain A  Khan MN  Iqbal Z  Sajid MS  Khan MK 《Veterinary parasitology》2011,179(1-3):92-99

Evaluation of anthelmintic effects of Trianthema (T.) portulacastrum L. (Aizoaceae) whole plant and Musa (M.) paradisiaca L. (Musaceae) leaves against prevalent gastrointestinal worms of sheep was done that may justify their traditional use in veterinary clinical medicine. In vitro anthelmintic activity of the crude aqueous methanolic extract (CAME) of both the plants was determined using mature female Haemonchus (H.) contortus and their eggs in adult motility assay (AMA) and egg hatch test (EHT), respectively. In vivo anthelmintic activity of crude powder (CP) and CAME in increasing doses (1.0-8.0 g kg(-1)) was determined in sheep naturally infected with mixed species of nematodes using fecal egg count reduction test (FECRT) and larval counts. The study design also included untreated as well as treated controls. Fecal egg count reduction and larval counts from coprocultures were performed pre- and post-treatments to assess the anthelmintic activity of the plants. CAME of T. portulacastrum and M. paradisiaca showed a strong in vitro anthelmintic activity and pronounced inhibitory effects on H. contortus egg hatching as observed through AMA and EHT, respectively. Both plants exhibited dose and time dependent anthelmintic effects on live worms as well as egg hatching. M. paradisiaca (LC(50)=2.13 μg mL(-1)) was found to be more potent than T. portulacastrum (LC(50)=2.41 μg mL(-1)) in EHT. However, in vivo, maximum reduction in eggs per gram (EPG) of faeces was recorded as 85.6% and 80.7% with CAME of T. portulacastrum and M. paradisiaca at 8.0 g kg(-1) on 15th day post-treatment, respectively as compared to that of Levamisole (7.5 mg kg(-1)) that caused 97.0% reduction in EPG. All the species of gastrointestinal nematodes (GINs), i.e. Haemonchus contortus, Trichostronglyus spp., Oesophagostomum columbianum and Trichuris ovis which were prevalent, found susceptible (P<0.01) to the different doses of CP and CAME of both plants. The data showed that both T. portulacastrum and M. paradisiaca possess strong anthelmintic activity in vitro and in vivo, thus, justifying their use in the traditional medicine system of Pakistan.  相似文献   

A Study on Seasonal Variability of Trace Elemental Status of Forages for Grazing Ruminants     

Zafar I. Khan  Muhammad Ashraf  Altaf Hussain  L. R. McDowell 《Journal of plant nutrition》2013,36(8):1345-1354

An investigation was conducted to determine the trace- mineral concentrations of forages in relation to requirements of ruminants grazing in natural pastures in the province of Punjab, Pakistan during two different seasons. Animals were closely followed during grazing and forages corresponding to those consumed by the animals were taken and analyzed for copper (Cu), iron (Fe), zinc (Zn), manganese (Mn), and cobalt (Co) concentrations. The data on the trace- mineral concentrations showed that most of these minerals varied greatly as a function of seasons and sampling periods. The forage Cu, Fe, and Zn concentrations were affected by seasonal changes but no influence of season was observed on the concentrations of forage Mn and Co concentration. Forage Zn and Co, during both seasons and at some sampling intervals, and forage Mn during summer were at marginal deficient levels, and in contrast, all other forage trace-minerals were within the required range for ruminants during both seasons. Based on these results, the supplementation of Zn, Co, and Mn would seem most important to support optimum livestock productivity.  相似文献   

<Emphasis Type="Italic">Rhizobium</Emphasis>,<Emphasis Type="Italic"> Bradyrhizobium</Emphasis> and<Emphasis Type="Italic"> Agrobacterium</Emphasis> strains isolated from cultivated legumes     

Sohail?Hameed  Sumera?Yasmin  Kauser?A.?Malik  Yusuf?Zafar  Fauzia?Y.?HafeezEmail author 《Biology and Fertility of Soils》2004,39(3):179-185

The present study was conducted to isolate and characterize rhizobial strains from root nodules of cultivated legumes, i.e. chickpea, mungbean, pea and siratro. Preliminary characterization of these isolates was done on the basis of plant infectivity test, acetylene reduction assay, C-source utilization, phosphate solubilization, phytohormones and polysaccharide production. The plant infectivity test and acetylene reduction assay showed effective root nodule formation by all the isolates on their respective hosts, except for chickpea isolate Ca-18 that failed to infect its original host. All strains showed homology to a typical Rhizobium strain on the basis of growth pattern, C-source utilization and polysaccharide production. The strain Ca-18 was characterized by its phosphate solubilization and indole acetic acid (IAA) production. The genetic relationship of the six rhizobial strains was carried out by random amplified polymorphic DNA (RAPD) including a reference strain of Bradyrhizobium japonicum TAL-102. Analysis conducted with 60 primers discriminated between the strains of Rhizobium and Bradyrhizobium in two different clusters. One of the primers, OPB-5, yielded a unique RAPD pattern for the six strains and well discriminated the non-nodulating chickpea isolate Ca-18 from all the other nodulating rhizobial strains. Isolate Ca-18 showed the least homology of 15% and 18% with Rhizobium and Bradyrhizobium, respectively, and was probably not a (Brady)rhizobium strain. Partial 16S rRNA gene sequence analysis for MN-S, TAL-102 and Ca-18 strains showed 97% homology between MN-S and TAL-102 strains, supporting the view that they were strains of B. japonicum species. The non-infective isolate Ca-18 was 67% different from the other two strains and probably was an Agrobacterium strain.  相似文献   

Sugar-Starch Metabolism and Antioxidant Potential in Potato Tubers in Response to Different Antisprouting Agents During Storage     

Kashif Sarfraz Abbasi  Tariq Masud  Sartaj Ali  Sami Ullah Khan  Talat Mahmood  Abdul Qayyum 《Potato Research》2015,58(4):361-375

Single applications of different antisprouting agents like hot water treatment, spearmint oil and clove oil were carried out on potato cultivar “Lady Rosetta” to compare their efficacy with that of synthetic chloro isopropyl N-phenyl carbamate (CIPC). The tubers were stored at ambient storage conditions (25?±?2 °C) for 81 days to assess changes in their sugar-starch concentrations and antioxidant potential. Antioxidant potential in the tubers was assessed as their total phenolic concentrations and radical scavenging activities. In addition, the enzymatic activities were also determined in order to evaluate the possible depletion of these antioxidants as substrate during storage. Results revealed significant response of stored potatoes to all antisprouting agents compared with the control (P?≤?0.05). CIPC and clove oil applications maintained tuber dormancy almost twice as long (81 days) as observed in the control (45 days). Application of spearmint oil and hot water treatment maintained tuber dormancy for almost 2 months. However, it was associated with an increased percentage sprouting during the last weeks of storage. At the end of storage, the highest starch (16.83%) and lowest sugar (0.99%) concentrations were estimated after CIPC application and maximum total phenolic concentration (143.57 mg gallic acid equivalent (GAE)/100 g), and highest antioxidant activity (39.73%) were found after clove oil application. Enzymatic activities were not statistically different between CIPC and clove oil application during most of the storage period. Results showed that efficient replacement of CIPC with clove oil in the premium potato cultivar might be useful; this may avert related food safety and environmental issues and would also ensure organic potato storage.  相似文献   

Effect of surface treatment of jute fibers on the interfacial adhesion in poly(lactic acid)/jute fiber biocomposites     

Mohammad Tahir Zafar  Saurindra Nath Maiti  Anup Kumar Ghosh 《Fibers and Polymers》2016,17(2):266-274

Jute fibers have immense potential to be used as natural fillers in polymeric matrices to prepare biocomposites. In the present study jute fibers were surface treated using two methods: i) alkali (NaOH) and ii) alkali followed by silane (NaOH+Silane) separately. Effects of surface treatments on jute fibers surface were characterized using fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) analyses. Further, the effects of surface treatments on jute fibers properties such as crystallinity index, thermal stability, and tensile properties were analyzed by X-ray diffraction method (XRD), thermo gravimetric analysis (TGA), and single fiber tensile test respectively. The effects of surface treatment of jute fibers on interphase adhesion between of poly(lactic acid) (PLA) and jute fibers were analyzed by performing single fiber pull-out test and was examined in terms of interfacial shear strength (IFSS) and critical fiber length.  相似文献   

Evaluation of Sustainable Cropping Sequences for Ameliorating Saline-Sodic Soil with Amendments and Water Having Different EC to SAR Ratios     

Ghulam Murtaza  Irshad Bibi  Umad Zafar Kahlon 《Communications in Soil Science and Plant Analysis》2016,47(18):2135-2147

A pot experiment was conducted on saline-sodic soil following sorghum-oat and rice-wheat crop rotations and amending with gypsum (G), farm manure (FM), and mulch (M). Water of different qualities viz. electrical conductivity (EC) 0.6 + sodium adsorption ration (SAR) 6, EC 1.0 + SAR 12 and EC 2.0 + SAR 18 was used for irrigation. The results showed that one pore volume (PV) of brackish water with higher EC and SAR ratio may be used beneficially, if proper amendments are applied. For rice and oat crops, the order of effective amendments was G>FM>M>control (C). With sorghum and wheat, the order was FM>G > M > C. Salt removal from soils was the lowest when leaching fraction (LF) was 0.20 and was the highest when LF was 0.32, i.e. a direct positive relationship. This proposes that irrigation water and the salt accumulated in the profile should be measured after short intervals to avoid reoccurrence of salinity.  相似文献   

Biosorption of Chromium (III) and Chromium (VI) by Untreated and Pretreated Cassia fistula Biomass from Aqueous Solutions     

Mazhar Abbas  Raziya Nadeem  Muhammad Nadeem Zafar  Mamoona Arshad 《Water, air, and soil pollution》2008,191(1-4):139-148

The present study explained the effect of pretreatments on the biosorption of Cr (III) and Cr (VI) by Cassia fistula biomass from aqueous solutions. For this purpose Cassia fistula biomass was pretreated physically by heating, autoclaving, boiling and chemically with sodium hydroxide, formaldehyde, gluteraldehyde, acetic acid, hydrogen peroxide, commercial laundry detergent, orthophosphoric, sulphuric acid, nitric acid, and hydrochloric acid. The adsorption capacity of biomass for Cr (III) and Cr (VI) was found to be significantly improved by the treatments of gluteraldehyde (95.41 and 96.21 mg/g) and benzene (85.71 and 90.81 mg/g) respectively. The adsorption capacity was found to depend on pH, initial metal concentration, dose, size, kinetics, and temperature. Maximum adsorption of both the Cr (III) and Cr (VI) was observed at pH 5 and 2. When Freundlich and Langmuir isotherms were tested, the latter had a better fit with the experimental data. The kinetic studies showed that the sorption rates could be described better by a second order expression than by a more commonly applied Lagergren equation.  相似文献   

A Comparative Study of Different Amendments on Amelioration of Saline-Sodic Soils Irrigated with Water Having Different EC: SAR Ratios     

Ghulam Murtaza  Umad Zafar Kahlon  Muhammad Yaseen 《Communications in Soil Science and Plant Analysis》2017,48(22):2630-2641

Soil degradation affects soil properties such as structure, water retention, porosity, electrical conductivity (EC), sodium adsorption ratio (SAR), and soil flora and fauna. This study was conducted to evaluate the response of contrasting textured soils irrigated with water having different EC:SAR ratios along with amendments: gypsum (G), farm manure (FM), and mulch (M). Water of different qualities viz. EC 0.6 + SAR 6, EC 1.0 + SAR 12, EC 2.0 + SAR 18, and EC 4.0 + SAR 30 was used in different textured soils with G at 100% soil gypsum requirement, FM at 10 Mg ha^?1, and M as wheat straw was added on surface soil at 10 Mg ha^?1. Results revealed that the applied amendments in soils significantly decreased pH_s and electrical conductivity (EC_e) of saturated paste and SAR. Four pore volumes of applied water with leaching fraction 0.75, 0.77, and 0.78 removed salts 3008, 4965, and 5048 kg ha^?1 in loamy sand, silty clay loam, and sandy clay loam soils, respectively. First four irrigations with LF of 0.82, 0.79, 0.75, and 0.71, removed 5682, 5000, 3967, and 2941 kg ha^?1 salts, respectively. The decreasing order for salt removal with amendments was FM > G > M > C with LF = 0.85, 0.84, 0.71, and 0.68, respectively. This study highlights a potential role of soil textures to initiate any mega program for reclamation of saline-sodic soils in the perspective of national development strategies.  相似文献   

Laboratory purification of fenitrothion     

J. Kova i ov  Zafar Masud  V. Btora  J. Kov   &#x;. Truchlik 《Pest management science》1971,2(3):101-102

A simple method for laboratory purification of fenitrothion technical concentrates for analytical purposes is described. The method is based on the differential rate of alkaline hydrolysis of fenitrothion, its S-methyl isomer and corresponding oxygen analogue. The volatile impurities are removed by counter-current steam distillation.  相似文献   

Emergence season of Hypoderma lineatum warbles in southern Punjab, Pakistan     

Muhammad Nisar KHAN  Muhammad Sohail SAJID  Zafar IQBAL  Muhammad ANWAR 《Animal Science Journal》2007,78(2):206-210

The objective of the present study was to observe the seasonal pattern of the life cycle and the warble stage in bovines of Dera Ghazi Khan and Rajan Pur Districts of Southern‐Punjab, Pakistan. The study was conducted from July 2004 to June 2005. The association was studied between various climatic changes and the fly life cycle. The larvae of warble flies were extracted from the backs of naturally infested animals and identified. The adult fly laid eggs in June, and hatched larvae penetrated the skin and followed intensive internal migration to diaphragm, esophageal wall and subcutaneous connective tissue in the month of July. At the start of August they reached the subcutaneous tissue and emergence began. In both species, the maximum warble emergence was found in mid‐December and the lowest in mid‐March and mid‐August. It was concluded that preventive treatment should be adopted before the month of July to avoid carcass depreciation and decreased leather quality.  相似文献