重组猪抑制素蛋白质中毒素残留检测及其理化稳定性     

李辉  翟颖超  窦晓  张甜  果双双  施振旦 《江苏农业学报》2015,31(2)

为探索纯化的重组猪抑制素蛋白质毒素残留情况以及重组猪抑制素蛋白质稳定性,本研究利用鲎试剂、气相色谱和SDS-PAGE方法对重组猪抑制素蛋白质中的细菌内毒素、β-巯基乙醇的残留量及其蛋白质在不同温度、极端p H值和反复冻融条件下的稳定性进行检测。结果显示,经过3次等电点沉淀洗涤后,重组猪抑制素蛋白质中细菌内毒素和β-巯基乙醇的残留量大幅减少。通过在不同温度下的处理发现,重组猪抑制素蛋白质在4℃环境下较稳定,可以长期保存;但在37℃时,稳定性快速下降,随着温度上升而降解加速。通过极端p H值和反复冻融试验发现,重组猪抑制素蛋白质对极端p H值和反复冻融具有较好的耐受性。  相似文献   

Vitamin C requirement of adult genetically improved farmed tilapia,Oreochromis niloticus     

Fan Wu  Feng Huang  Hua Wen  Ming Jiang  Wei Liu  Juan Tian  Chang Geng Yang 《Aquaculture International》2015,23(5):1203-1215

  相似文献   

Development of quantification bioassays for live propagules of Phytophthora sojae in soil     

Bing Suo  Caixia Xiao  Haocheng Lin  Qiuming Chen  Liming Zhao  Miao Tian  Qianqian Sun  Jingzhi Wen 《Journal of General Plant Pathology》2015,81(4):271-278

  相似文献   

新疆兵团主要植棉区机采棉田杂草调查   总被引：1，自引：1，他引：0  

张强  田英  朱玉永  马江锋  李红 《中国棉花》2020,47(5):13-16

采用倒置“W”九点取样法对新疆兵团北疆、东疆主要植棉师蕾期、铃期棉田杂草进行调查。结果表明：北疆第六师、东疆第十三师机采棉田杂草主要有16科31种，其中禾本科杂草占16.1%，阔叶杂草占80.7%，莎草科杂草占3.2%；1年生杂草占64.5%，多年生杂草占35.5%。北疆第六师蕾期棉田优势杂草为龙葵、野西瓜苗、田旋花、灰藜，铃期优势杂草为龙葵、田旋花、灰藜。东疆第十三师蕾期棉田优势杂草为灰藜、反枝苋、狗尾草，铃期优势杂草为龙葵、灰藜、反枝苋、田旋花。不同地区、不同生育时期棉田优势杂草有所差异。研究结果将为新疆兵团机采棉田杂草的科学可持续防控提供重要依据。  相似文献   

Influences of sand cover on erosion processes of loess slopes based on rainfall simulation experiments   总被引：1，自引：0，他引：1  

ZHANG Xiang  LI Zhanbin  LI Peng  TANG Shanshan  WANG Tian  ZHANG Hui 《干旱区科学》2018,10(1):39-52

Aeolian-fluvial interplay erosion regions are subject to intense soil erosion and are of particular concern in loess areas of northwestern China.Understanding the composition,distribution,and transport processes of eroded sediments in these regions is of considerable scientific significance for controlling soil erosion.In this study,based on laboratory rainfall simulation experiments,we analyzed rainfall-induced erosion processes on sand-covered loess slopes(SS)with different sand cover patterns(including length and thickness)and uncovered loess slopes(LS)to investigate the influences of sand cover on erosion processes of loess slopes in case regions of aeolian-fluvial erosion.The grain-size curves of eroded sediments were fitted using the Weibull function.Compositions of eroded sediments under different sand cover patterns and rainfall intensities were analyzed to explore sediment transport modes of SS.The influences of sand cover amount and pattern on erosion processes of loess slopes were also discussed.The results show that sand cover on loess slopes influences the proportion of loess erosion and that the compositions of eroded sediments vary between SS and LS.Sand cover on loess slopes transforms silt erosion into sand erosion by reducing splash erosion and changing the rainfall-induced erosion processes.The percentage of eroded sand from SS in the early stage of runoff and sediment generation is always higher than that in the late stage.Sand cover on loess slopes aggravates loess erosion,not only by adding sand as additional eroded sediments but also by increasing the amount of eroded loess,compared with the loess slopes without sand cover.The influence of sand cover pattern on runoff yield and the amount of eroded sediments is larger than that of sand cover amount.Furthermore,given the same sand cover pattern,a thicker sand cover could increase sand erosion while a thinner sand cover could aggravate loess erosion.This difference explains the existence of intense erosion on slopes that are thinly covered with sand in regions where aeolian erosion and fluvial erosion interact.  相似文献   

农学专业拔尖创新型人才培养模式改革与实践——以湖南农业大学为例          下载免费PDF全文

傅志强  高志强 《农业工程》2019,9(5):102-105

以培养拔尖创新型人才为目标，在人才培养体系构建、人才培养制度建设以及人才培养工作制度等方面进行了一系列改革，通过遴选组建农学隆平创新实验班进行实践探索，结果表明，模式改革在学生科研素养、创新意识和德智体美劳等方面成效显著，特别是协同培养和连续培养在拔尖创新型人才培养过程中作用尤其突出，可资借鉴。   相似文献   

Genetic diversity evaluation of winged bean (Psophocarpus tetragonolobus (L.) DC.) using inter-simple sequence repeat (ISSR)     

Dongming Chen  Xing Yi  Hui Yang  Hui Zhou  Ye Yu  Yun Tian  Xiangyang Lu 《Genetic Resources and Crop Evolution》2015,62(6):823-828

  相似文献   

我国玉米品种标准DNA指纹库构建研究及应用进展   总被引：2，自引：0，他引：2  

赵久然  王凤格  易红梅  田红丽  杨扬 《作物杂志》2015,(2):1-6,170

介绍了我国玉米品种标准DNA指纹库构建的进展及应用情况。我国玉米品种标准DNA指纹库规模已达到22 089份,经与国内外作物品种DNA指纹库比较,是全球品种数量和建库规模最大的DNA指纹库,并且在区域试验、品种权保护及种子质量管理等方面都得到了广泛应用。至今已开展玉米品种真实性鉴定等达40 000多份次。同时,对DNA指纹库构建未来发展进行预测,建议继续发挥SSR标记的作用、加快推进SNP标记的研发、发掘In Del标记的应用潜力并持续关注测序技术的发展。  相似文献   

两种除草剂对紫背浮萍的生长抑制活性研究   总被引：2，自引：0，他引：2  

田延辉  姜辉  黄继光  张燕  瞿唯钢 《农药科学与管理》2015,(3):61-65

本文以叶状体数为测试指标,在实验室条件下研究了除草剂草甘膦、二甲戊灵对紫背浮萍生长的影响。结果表明:在一定范围内,两种除草剂均对紫背浮萍的生长具有明显的抑制作用,抑制率都随浓度的增加而增大,处理168h后,草甘膦、二甲戊灵对紫背浮萍叶状体的EC50分别为12.817、0.483mg/L。  相似文献   

浅谈石屏县木材运输管理存在的问题与对策     

李志强 《林业建设》2015,(1):4-6

本文针对石屏县木材运输管理的现状及存在问题,提出解决问题的对策,达到有效保护森林资源的目的。  相似文献