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991.
Equilibrium radionuclide ventriculography was applied in 27 healthy anaesthetised dogs (9 to 60 kg) after labelling the blood pool with 99mTc, using a gamma camera with a computer on-line. Quantitative analysis of the calibrated ventriculogram provided the following left ventricular parameters: the end diastolic volume (EDV), the ejection fraction (EF), the possible degree of regurgitation and as velocity indices the peak ejection and peak refilling rates (PER and PRR). Averaged results were: EDV 1.8 ml kg-1; EF 0.59; PER 4.3 EDV s-1; PRR 3.75 EDV s-1. Visual inspection of the camera images may reveal abnormal wall motions and dilatation of heart chambers. 相似文献
992.
Investigation of an outbreak of mucosal disease in a beef cattle herd in southwestern Saskatchewan. 总被引:2,自引:2,他引:0 下载免费PDF全文
L F Taylor J Van Donkersgoed O M Radostits C W Booker E J Dubovi J V van den Hurk E D Janzen 《The Canadian veterinary journal. La revue veterinaire canadienne》1994,35(7):425-432
This study describes the epidemiological investigation of an outbreak of mucosal disease that occurred on a ranch in southwestern Saskatchewan. Over a six-month period during the fall and winter of 1991-1992, in a herd of 515 beef cattle and 96 bison, 20 yearling cattle from a group of 105 housed in one feedlot pen died from mucosal disease. A further eight yearlings were slaughtered for salvage because they were at risk of dying from mucosal disease. Mucosal disease mortalities were the first observed evidence of fetal infections with bovine viral diarrhea virus in this herd. Animals that died from mucosal disease exhibited signs of ill thrift prior to death. Deaths from mucosal disease were confined to the progeny of one herd of beef cows. Following an outbreak of fetal infection with bovine viral diarrhea virus during 1989-1990, at least 28 (22%) of the 128 calves born from this herd of cows in the spring of 1990 were persistently infected with bovine viral diarrhea virus. However, only one calf born from this herd in 1991, and five calves born from all herds in 1992 were persistently infected. Of the five persistently infected calves born in 1992, three were born to persistently infected replacement heifers born in 1990. These heifers calved without assistance in 1992, but only one of their calves survived past three days of age, and it was persistently infected. In January 1992, 82% of the total herd had reciprocal antibody titers to bovine viral diarrhea virus of > or = 1024 which suggested a high level of herd immunity to bovine viral diarrhea virus.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
993.
Two products that contain CaCl2 and that are intended for oral administration were tested for safety aspects. One product contained CaCl2 as a gel, the other contained CaCl2 in an oil emulsion. Oral administration of the CaCl2-containing products caused minor to severe damage to the mucosa of the forestomach and abomasum. The gel solution especially proved to be highly caustic, while the oil emulsion appeared to be relatively safe. 相似文献
994.
Seroepidemiology of Breda virus in cattle using ELISA 总被引:3,自引:0,他引:3
Two direct blocking enzyme linked immunosorbent assays (ELISA) for the detection of antibodies to Breda virus in sera of cattle were compared. An ELISA with consecutive addition of antigen and test serum to an antibody-coated plate gave higher positive: negative absorbence ratios than an ELISA in which antigen and test serum were added simultaneously. Sera collected from breeding and fattening herds in The Netherlands (n = 1313) and the F.R.G. (n = 716) were tested, and antibodies to Breda virus were demonstrated in 94% of adult cattle. Ninety percent of newborn calves had high levels of maternal antibodies, which waned until the age of 3 months. Active seroconversion occurred between 7 and 24 months in most animals. 相似文献
995.
Adriaan den Hertog 《Veterinary research communications》1986,10(1):335-340
The motility of the mammalian gastro-intestinal tract is regulated by a number of neuronal systems. The postganglionic, intramural parts of these systems are characterized by their neurotransmitters as cholinergic, adrenergic and purinergic nerves, respectively. The transmitter acetylcholine evokes contractions of the smooth muscle cell, whereas both noradrenalin and the transmitter from the purinergic nerves, presumably ATP, induce relaxations of smooth muscles. Agents and actions which influence the neurohumoral transmission and the mechanisms underlying the effects induced by the transmitters in smooth muscle cells are reviewed. 相似文献
996.
J. Nuytten E. Muylle W. Oyaert C. van den Hende 《Veterinary research communications》1986,10(1):453-461
Pulmonary function tests were performed in six healthy calves. Prostaglandin F2 causes severe narrowing of both upper and lower airways (total lung resistance increased, dynamic compliance decreased). Clenbuterol administered intravenously fifteen minutes prior to prostaglandin F2 aerosol, and in increasing doses (0, 0.4, 0.8, 1.2 g/kg), on days 1, 2, 4 and 6 of the experiment, effectively but not entirely suppressed these responses.These data indicate that -adrenergic receptors are present in the bovine airways and that the use of clenbuterol (0.8 g/kg) may be effective in treating clinical respiratory disease such as bronchopneumonia in calves. 相似文献
997.
Bovine leukaemia: facts and hypotheses derived from the study of an infectious cancer 总被引:6,自引:0,他引:6
A Burny Y Cleuter R Kettmann M Mammerickx G Marbaix D Portetelle A van den Broeke L Willems R Thomas 《Veterinary microbiology》1988,17(3):197-218
Bovine leukaemia virus (BLV) is the etiological agent of chronic lymphatic leukaemia/lymphoma in cows, sheep and goats. Infection without neoplastic transformation was also obtained in pigs, rhesus monkeys, chimpanzees, rabbits and observed in capybaras and water-buffaloes. Structurally and functionally, BLV is a relative of human T lymphotropic viruses 1 and 2 (HTLV-I and HTLV-II) In humans, HTLV-I induces a T-cell leukaemia and its type 2 counterpart has been found in dermatopathic lymphadenopathy, hairy T-cell leukaemia and prolymphocytic leukaemia cases. At variance with HTLV-I, BLV has not been associated with neurological diseases of the degenerative type. Bovine leukaemia virus, HTLV-I and HTLV-II show clearcut sequence homologies. The pathology of the BLV-induced disease, most notably the absence of chronic viraemia, a long latency period and lack of preferred proviral integration sites in tumours, is similar to that of adult T-cell leukaemia/lymphoma induced by HTLV-I. The most striking feature of these three naturally transmitted leukaemia viruses is the X region located between the env gene and the long terminal repeat (LTR) sequence. The X region contains several overlapping long open reading frames. One of them, designated XBL-I, encodes a trans-activator function capable of increasing the level of gene expression directed by BLV-LTR and most probably is involved in "genetic instability" of BLV-infected cells of the B cell lineage. The "genetic instability" renders the infected cell susceptible to move, along a number of stages, towards full malignancy. Little is known about these events and their causes; we present some theoretical possibilities. Bovine leukaemia virus infection has a worldwide distribution. In temperate climates, the virus spreads mostly via iatrogenic transfer of infected lymphocytes. In warm climates and in areas heavily populated by haematophagous insects, there are indications of insect-borne propagation of the virus. 相似文献
998.
M. Leeman J. A. van Pelt F. M. den Ouden M. Heinsbroek P. A. H. M. Bakker B. Schippers 《European journal of plant pathology / European Foundation for Plant Pathology》1995,101(6):655-664
Pseudomonas fluorescens-mediated induction of systemic resistance in radish against fusarium wilt (Fusarium oxysporum f. sp.raphani) was studied in a newly developed bioassay using a rockwool system. In this bioassay the pathogen and bacterium were confirmed to be confined to spatially separate locations on the plant root, throughout the experiment. Pathogen inoculum obtained by mixing peat with microconidia and subsequent incubation for four days at 22 °C, yielded a better percentage of diseased plants than a microconidial suspension drench, an injection of a microconidial suspension into the hypocotyl, or a talcum inoculum.Pseudomonas fluorescens strain WCS374 applied in talcum or peat, but not as a suspension drench, induced systemic resistance. A minimal initial bacterial inoculum density of 105 CFU WCS374 root–1 was required to significantly reduce the percentage diseased plants. At least one day was necessary between bacterization of strain WCS374 in talcum on the root tips and inoculation of the pathogen in peat on the root base, for an optimal induction of systemic resistance. Strain WCS374 induced systemic resistance in six radish cultivars differing in their susceptibility toF. oxysporum f. sp.raphani. Significant suppression of disease by bacterial treatments was generally observed when disease incidence in the control treatment, depending on pathogen inoculum density, ranged between approximately 40 to 80%. Strains WCS374 and WCS417 ofPseudomonas fluorescens induced systemic resistance against fusarium wilt, whereasP. putida WCS358 did not. This suggests that the induction of systemic resistance byPseudomonas spp. is dependent on strain-specific traits.Abbreviations CFU
colony forming units
- IFC
immunofluorescence colony-staining
- ISR
induced systemic resistance
- PBS
phosphate buffered saline
- SAR
systemic acquired resistance 相似文献
999.
Performance, survival, necropsy, and virological findings from calves persistently infected with the bovine viral diarrhea virus originating from a single Saskatchewan beef herd. 总被引:3,自引:1,他引:2 下载免费PDF全文
L F Taylor E D Janzen J A Ellis J V van den Hurk P Ward 《The Canadian veterinary journal. La revue veterinaire canadienne》1997,38(1):29-37
Fifty-one calves from 652 cows and heifers that calved on a Saskatchewan ranch in 1992 were identified as persistently infected with bovine viral diarrhea virus (BVDV), based on virological and necropsy findings. Herd records suggested a further 20 calves that died between birth and weaning were probably also persistently infected. Subsequent to weaning, all surviving persistently infected calves were transferred to one pen in a 10,000 head commercial feedlot, to mimic normal management practice in western Canadian beef herds. On average, when compared with healthy, BVDV-negative herdmates, persistently infected calves were "poor doers" and had poor survivability, with only 4 persistently infected calves surviving to 1 year of age. There was no difference (P > 0.05) in survival between male and female persistently infected calves. The clinical, pathological, and virological findings from these persistently infected calves varied over time. The majority of persistently infected calves had gross pathological lesions at necropsy, consistent with mucosal disease. However, approximately 25% of the persistently infected calves had gross pneumonic lesions at necropsy, with no or only mild lesions of mucosal disease. A wide variety of other lesions were also noted in persistently infected calves at necropsy. Therefore, the possibility that BVDV-induced lesions can be misdiagnosed is very real. The results of this study indicate that persistent infection with BVDV should always be considered in calves with chronic ill thrift, chronic enteritis, or respiratory disease. 相似文献
1000.
ABSTRACT The inheritance of resistance to Leptosphaeria maculans, the causal agent of black leg of crucifers, was studied in Brassica juncea. Three resistant accessions (UM3021, UM3043, and UM3323) and one susceptible accession (UM3132) of B. juncea were crossed in a complete diallel. Parents, F(1), and F(2) progenies were evaluated for all crosses using both cotyledon and stem inoculation. Cotyledon reaction was evaluated with two isolates of L. maculans, but stem reaction was evaluated with one isolate. Disease reactions observed for individual plants were the same for both inoculation methods and for both isolates of the pathogen for cotyledon reaction. No segregation was observed for the crosses between resistant accessions (UM3043 x UM3323 and UM3021 x UM3323), but a few susceptible plants were observed in the F(2) progeny of crosses between resistant parents (UM3021 x UM3043). This was probably due to heterozygosity in some parental plants of UM3021. For crosses be tween the susceptible parent and resistant parents, F(1) plants for two crosses were all resistant. For cross UM3132 x UM3021, some susceptible plants occurred, which was also suggestive of heterozygosity in UM3021. Although resistance in F(1) was dominant, for F(2) populations, segregation fit either 13:3, 3:1, or 1:3 ratios, indicating that resistance can be either adominant or recessive trait. F(3) families derived from some susceptible F(2) plants from crosses UM3021 x UM3132 and UM3043 x UM3132 were evaluated using the cotyledon inoculation method only. Segregation of F(2) plants and F(3) families in crosses involving resistant and susceptible parents indicated that the resistance to L. maculans in B. juncea is controlled by two nuclear genes with dominant recessive epistatic gene action. 相似文献