金黄色葡萄球菌对BV2细胞IFN-α生成的影响     

卢婉青  赵莎莎  蒋松宏  童智子  黄丹妮  郭建华  吴俊伟  周洋 《畜牧兽医学报》2022,53(8):2633-2641

旨在探究金黄色葡萄球菌（Staphylococcus aureus，简称金葡菌）对小胶质细胞α干扰素（interferon α，IFN-α）生成的影响，本研究先用金葡菌感染BV2细胞，检测IFN-α的mRNA表达、分泌到细胞培养液上清的量以及TBK1/IRF3通路的激活情况，再分别用TBK1抑制剂BX-795和amlexanox、NF-κB抑制剂IMD-0354处理细胞，检测TBK1/IRF3的激活以及IFN-α水平。结果表明，BV2感染金葡菌后，IFN-α转录水平在3~12 h升高，6~12 h释放量增加，呈剂量依赖性，TBK1和IRF3的转录水平和蛋白水平未发生变化，但在1~12 h其磷酸化水平升高，表明金葡菌激活BV2细胞TBK1/IRF3通路，BX-795、amlexanox和IMD-0354处理细胞后，IRF3磷酸化水平降低，IFN-α生成减少，表明TBK1和NF-κB参与金葡菌促进BV2细胞生成IFN-α。综上所述，金葡菌感染BV2后促进IFN-α生成，且该过程依赖于TBK1和NF-κB，本结果为临床防治中枢神经系统感染金葡菌提供了可能靶点和理论基础。  相似文献   

Experiential legacies of early-life dietary polyunsaturated fatty acid content on juvenile Walleye: Potential impacts from climate change     

L. Zoe Almeida  John Grayson  Stuart A. Ludsin  Konrad Dabrowski  Elizabeth A. Marschall 《Ecology of Freshwater Fish》2023,32(1):23-36

Climate-induced shifts in plankton blooms may alter fish recruitment by affecting the fatty acid composition of early-life diets and corresponding performance. Early-life nutrition may immediately affect survival but may also have a lingering influence on size and growth via experiential legacies. We explored the short- and longer-term performance consequences of different concentrations of polyunsaturated fatty acids (PUFA) for juvenile Walleye (Sander vitreus, Mitchill 1818). For the first 10 days of feeding, juveniles were provided Artemia enriched with: oleic acid (low PUFA), high docosahexaenoic acid and high eicosapentaenoic acid (high PUFA), or high PUFA and a form of vitamin E (high PUFA + E). After 10 days, all fish were fed a high-quality diet and reared for an additional 27 days. Juveniles fed either high PUFA diet were 1.15-fold larger (PUFA mean ± SD = 20.0 ± 3.3 mg; PUFA + E = 19.8 ± 3.3 mg) than those fed the low PUFA (17.3 ± 2.8 mg) diet after 10 days of feeding. After 27 days, juveniles initially fed the high PUFA diet were still 1.10- to 1.20-fold larger (PUFA = 407.0 ± 61.6 mg; PUFA + E = 422.7 ± 58.7 mg) than those initially fed the low PUFA diet (356.5.0 ± 39.5 mg). Our findings demonstrate that fatty acid composition of juvenile Walleye diets has immediate and lingering size effects. As changes in climate continue to alter lower trophic levels, fish management and conservation may need to consider short- and long-term effects of temporal or spatial differences in early-life diet quality.  相似文献