珍珠豆型花生品种白沙1016核型分析   总被引：1，自引：0，他引：1  

杜培  张新友  李丽娜  黄冰艳  董文召  汤丰收 《中国油料作物学报》2013,35(3)

珍珠豆型花生白沙1016是重要的花生种质资源.利用高度保守的重复序列5S和45SrDNA作探针对白沙1016根尖细胞中期染色体进行荧光原位杂交(FISH)分析,发现两探针在白沙1016的12条染色体上能产生杂交信号,其中1对染色体长臂与短臂、2对染色体短臂、3对染色体长臂具有杂交信号.综合DAPI+染色带纹和染色体长度、臂比、面积等特征,可以将白沙1016大部分染色体区分开,为白沙1016的育种利用提供了染色体遗传分析基础.  相似文献   

海南及粤西外来入侵植物假臭草染色体核型分析     

张颖  黎丽倩  刘强 《草地学报》2019,27(1):274-278

对海南岛及粤西地区8个样区,3种生境（旷野、林缘和林中）假臭草（Praxelis clematidea）的染色体核型进行分析。结果表明,染色体均为二倍体,染色体数目2n=28。染色体核型表现为2A,2B,3B和3A。核型属较不对称类型,不同居群假臭草的居群亲缘关系与地理分布不完全一致,说明假臭草在入侵和传播过程中受到环境因子和人为干扰的影响较大。  相似文献   

Teratoma Associated With Testicular Tissue in a Female-Like Horse With 64,XY (SRY-Positive) Disorder of Sex Development     

《Journal of Equine Veterinary Science》2020

A four-year-old female-like Quarter Horse was admitted for clinical evaluation because of masculinized-aggressive behavior and lack of estrous signs. On external inspection, a hypertrophied clitoris and prominent muscles were observed. On gynecological examination, apparently normal mammary glands, vulva, vagina, and cervix were noted. On the other hand, the uterus had no tone and was smaller than normal. The left gonad was very soft on palpation and the right gonad was mostly firm, irregular, and wider than the left gonad. On ultrasound examination, there were no signs of regular ovarian structure or follicular activity. Because of the different shapes and consistencies of the gonads and a suspicion of increased testosterone production, a bilateral gonadectomy was recommended. Blood was collected for testosterone levels quantification and for cytogenetic and molecular investigations. After removal, gonads were analyzed macroscopically and sections were sent for histopathological examination. A final diagnosis of benign adult teratoma associated with seminiferous tubules and Leydig cells was made. On cytogenetic and molecular analyses, a normal diploid number of 64 chromosomes and the presence of the XY sex chromosomes were seen in all cells, as well as the SRY gene. Testosterone levels were higher than normal before surgery and were reduced after gonads removal. In conclusion, the masculinized behavior was probably caused by increased testosterone levels produced by testicular tissue, in a female-like horse with 64,XY SRY-positive disorder of sex development, which was associated with a teratoma.  相似文献   

黑果枸杞染色体核型分析   总被引：4，自引：0，他引：4  

陈海魁  曹君迈  任贤  黄素平  张爽 《北方园艺》2008,(7)

采用染色体常规制片法,结合显微摄影技术对黑果枸杞染色体进行检测分析,结果表明:黑果枸杞染色体数为2n=24,核型公式是2n=24=20m 2sm 2m(sat),全组染色体总长度为86.88 μm,长臂总长49.44 μm,核型不对称系数(AS.K%)为56.9%,总体积为127.58 μm3.  相似文献   

Study on Karyotype of Hobby (Falco subbuteo) and Kestrel (Falco tinnunculus)     

王明力  陈凤英 《林业研究》1998,(2)

IntroductionHobbiesandKestrelsaremiddleandsmallbirdsofpreyinFalconiformes,withverywidedistributioninChina,livingindifferenttypesofforests,shrubbery's,forestedges,waterareasornearinhabitants.Theylivesolitarilyorinpairs,flyveryrapidlyandpreyonsmallmammalssuchasrodents,youngbirdsandalsoinsects.Theirfoodhabitisverybeneficialtothepreservationofforestsandtheregulationoftherelativeequilibriumofforestecosystems,sothattheyarelistedintheprotectedanimalsClasslllilBirdsofFalconiformesinChinaareclassifi…  相似文献   

Study on karyotype of Hobby (Falco subbuteo) and Kestrel (Falco tinnunculus)     

Wang Mingli  Chen Fengying 《林业研究》1998,9(2):108-110

This paper reported karyotypes of the Hobby and Kestrel collected from the suburb of Qiqihaer City. These karyotypes were compared and analyzed, on the basis of the study of karyotypes of nine birds species in Falconiformes by Bian Xiaozhuang and Li Qingwei. It was considered that differences in habits of birds and ecological environment not only cause falcons to differ from others, but also differences in a genus, even within a species. This difference may result from fusion of microchromosomes.  相似文献   

苏联球茎大麦、（拟）智利大麦和普通大麦的核型比较研究     

管启良  张毓芳  茅国夫 《中国农业科学》1988,21(2):32-35

本文对苏联球茎大麦、（拟）智利大麦和普通大麦的核型进行了比较研究。苏联球茎大麦的核型为K=28=24m+4m#+（SAT）;（拟）智利大麦的核型为K=14=10m+4sm#+（SAT）;普通大麦的核型为K=14=10m+4sm#+（SAT）。  相似文献   

家牦牛与不同野血牦牛染色体组型的比较分析   总被引：3，自引：0，他引：3  

张周平 《青海畜牧兽医杂志》1998,28(6):15-17

本文用外周血淋巴细胞培养法制备染色体标本,比较分析了家牦牛、１／２野血牦牛及３／４野血牦牛的染色体组型。结果表明：家牦牛、１／２野血牦牛和３／４野血牦牛的二倍体细胞染色体数目均为２ｎ＝６０,常染色体为近端着丝粒,性染色体为亚中着丝粒;家牦牛和野牦牛的染色体组型无明显差异。  相似文献   

高寒鲤染色体核型分析   总被引：5，自引：2，他引：3  

白庆利刘明华  尹洪滨徐伟 《水产学杂志》1999,12(1):47-49

采用植物血球凝集素（ＰＨＡ）体内注射,肾组织细胞短期培养,常规空气干燥法制备了高寒鲤染色体,核型分析结果是：高寒鲤染色体的核型公式为２ｎ＝１００＝２８ｍ＋２８ｓｍ＋４４ｓｔｔ,染色体臂数ＮＦ＝１５６。  相似文献   

棕点石斑鱼(Epinephelus fuscoguttatus♀)×鞍带石斑鱼(Epinephelus lanceolatus♂)F1染色体制备方法及核型分析          下载免费PDF全文

刘莉  张岩  陈超  李炎璐  孔祥迪  于欢欢  陈建国  翟介明 《渔业科学进展》2016,37(2):33-40

探索了在鱼类大小规格不同情况下制备鱼类染色体的最佳策略和方法,并对棕点石斑鱼(Epinephelus fuscoguttatus♀)×鞍带石斑鱼(Epinephelus lanceolatus♂)杂交子代(俗称珍珠龙胆)的染色体核型进行研究.本研究根据预实验的结果,对所选取的珍珠龙胆幼鱼,采用小鱼游泳法进行前处理,取其鳍条并以冷、热两种不同的滴片方法制备染色体,观察染色体形态并对其染色体核型进行分析.结果显示,选用的染色体制备方法能获得图像清晰、形态良好的细胞分裂相;不同的滴片处理方法细胞分裂指数存在一定差异,即热滴片法的有丝分裂指数为2.58％,明显高于冷滴片法的有丝分裂指数(0.83％),此外,热滴片法所获得的染色体的质量也明显优于冷滴片法.珍珠龙胆石斑鱼二倍体染色体数为48,22对染色体为端部着丝粒染色体,2对染色体为亚端部着丝粒染色体,其染色体臂数(NF)为52,核型公式为2n=48,4st+44t.珍珠龙胆与父本鞍带石斑鱼的染色体数目、核型基本一致,而与母本棕点石斑鱼的核型存在很大差异.研究表明,珍珠龙胆的2n数与其亲本相同,其核型特点符合典型的高位类群鱼类核型特征.在对珍珠龙胆与其亲本的核型进行比较的同时,对已报道的25种石斑鱼进行了核型的比较和综合分析,为石斑鱼的种质鉴定、遗传资源保护利用、育种提供基础资料和理论依据.  相似文献