不同方法提取三种生态型沙地云杉总DNA的比较   总被引：1，自引：0，他引：1  

邹春静  张超  马永亮  徐文铎 《内蒙古林业科技》2006,32(3):35-38

沙地云杉为内蒙古特有树种,干旱适应性强,是我国西部重要造林树种。在长期适应干旱生态条件的过程中沙地云杉形成了紫果型、绿果型和红果型3种生态型。前期研究证明:紫果型沙地云杉有更强的抗旱能力,绿果型抗旱能力弱,红果型处于二者之间,是一个比较典型的过渡类型。本文从分子生物学的角度,详细叙述了沙地云杉总DNA的提取过程、方法,并对已知的植物DNA提取方法作了针对性的改良;对实验过程提取DNA的影响作了分析。为今后针对进行沙地云杉的植物基因研究和生物工程打下基础,对扩大沙地云杉造林面积和西部大开发的生态环境建设具有重要的指导意义。  相似文献   

The regeneration characteristics of Picea jezoensis and Abies sachalinensis on cut stumps in the sub-boreal forests of Hokkaido Tokyo University Forest     

Masahiko Nakagawa  Akio Kurahashi  Taizo Hogetsu 《Forest Ecology and Management》2003,180(1-3):353-359

The regeneration characteristics of Picea jezoensis Carr. and Abies sachalinensis Masters on cut stumps in natural forest under selection cutting and natural regeneration were studied in a sub-boreal forest of the Hokkaido Tokyo University Forest. The following points were investigated: (1) what decay class of cut stumps are suitable for regeneration of the two conifer species; (2) what part, either a root collar or a cut surface, of a cut stump is suitable for regeneration; and (3) what percentages of cut stumps support conifer seedlings. The percentages of cut stumps that supported conifer seedlings increased as the decay class advanced, but slightly decreased in the latest stage of decay class, V. Both P. jezoensis and A. sachalinensis seedling densities per cut stump and per square meter of cut stumps increased as the decay class advanced. Some root collars in decay class IV supported A. sachalinensis seedlings, but few supported P. jezoensis. Densities of P. jezoensis per cut surface and per square meter of cut surface in decay class IV were higher than those of A. sachalinensis. On the other hand, densities of A. sachalinensis per root collar and per square meter of root collar in decay class IV were higher than those of P. jezoensis. Cut stumps in advanced decay classes are more suitable for regeneration of P. jezoensis and A. sachalinensis than those in early decay classes. Whereas a cut surface is more suitable for regeneration of P. jezoensis, a root collar is more suitable for regeneration of A. sachalinensis. Cut stumps are not sufficient to ensure enough conifer regeneration because not all conifer cut stumps in advanced decay classes support conifer seedlings.  相似文献   

Growth change of young Picea sitchensis in response to deer browsing     

Bruno Vila  Franck Torre  Frdric Guibal  Jean-Louis Martin 《Forest Ecology and Management》2003,180(1-3):413-424

Taking advantage of the introduction of the black-tailed deer to the Queen Charlotte Islands (British Columbia, Canada), we used dendrochronological analyses to understand the consequences of deer browsing on Sitka spruce growth. We compared shape, radial growth, height growth and age of young spruce in three sites. We identified two types of trees growing side by side: (1) stunted and heavily browsed spruce, smaller than the browsing limit and (2) escaped spruce that were taller than the browsing limit but still browsed in their lower part. The compact and heavily ramified shape in stunted spruce was the result of repeated and intense browsing. In escaped spruce this was also the case below the browsing limit (1.16 m±0.07 m), in sharp contrast with the normal shape that escaped spruce resumed above the browsing limit. We show that the release of browsing pressure, once the tree reaches the browsing limit, is characterised by an abrupt increase in radial growth. Before release, trees show a growth stagnation characterized by narrow rings (0.5 mm per year) and small annual height increments (<5 cm per year). After release, trees show a growth stabilisation characterised by wider rings (3 mm per year) and larger annual height increments (20 cm per year). We use this pattern to estimate frequency and age at release and their possible variation over time. Age differences between stunted and escaped spruce are highly significant and indicate that, despite of browsing, most if not all trees will ultimately reach the browsing limit and escape. Heavy deer pressure (30 deer per km²) delays spruce sapling recruitment by about 8 years. This delay varies in relation to site quality and seems to have increased over time, suggesting an increase in browsing pressure.  相似文献   

CONTRAST STUDY ON ROOTSYSTEM STRUCTURE OF THE FOUR ANNUAL CONIFEROUS SEEDLINGS IN CHIAN NORTHEAST AREA     

赵垦田  王录 《林业研究》1994,5(2):71-79

Rootsystemstructureofseedlingsisoneoftheimportantbiologicalcharacte-risticsoftrees.Duringtheseedlingculture,rootsystemstructureisrelatedwithmanyculturingseedlingtechnologies(suchassoiltillage,applyingfertilizer,irrigation,etc.).Comparingthedifferenceoftherootsystemstructureofdifferentconiferousseedlingscanhelpintensivefarmingandmanagementinnursery.Therefore,westudiedtherootsystemstructureoftheannualseedlingsoffourmainconifers(Pinuskoraiensis,Pinussylvestrisvar.mongoIica,LarixolgensisandPicea…  相似文献   

云杉树叶象幼虫空间分布型及其抽样技术研究   总被引：3，自引：0，他引：3  

李进军  马存世  邓军  李丽蓉 《甘肃林业科技》2007,32(3):4-8

为调查云杉树叶象幼虫在云杉S2-2、S2-3、S2-4苗龄型苗木上空间分布型及开展抽样技术调查研究,在云杉苗木中共设置了30块样地对该幼虫进行了调查。通过应用数理统计方法分析后得出:该幼虫有虫株率分布在云杉S2-2苗龄型苗木上呈对数函数分布趋势;在云杉S2-3、S2-4苗龄型苗木上呈指数函数分布趋势。应用聚集指标法分析得出:该幼虫空间分布型属聚集分布,并通过Iwao回归分析法和Toylor幂法则分析得出,云杉S2-2、S2-3苗龄型苗木上的幼虫个体间相互排斥;云杉S2-4苗龄型苗木上的幼虫个体间相互吸引。同时,应用Iwao的m*-m关系和序贯抽样方法,确定出了该幼虫的理论抽样数及抽样上限值和下限值。  相似文献   

Phenology Differs among Norway Spruce Populations in Relation to Local Variation in Altitude of Maternal Stands in the Beskidy Mountains     

Daniel J. Chmura 《New Forests》2006,32(1):21-31

An investigation of phenological and growth traits was conducted in a lowland plantation, comprised of 23 Norway spruce (Picea abies (L.) Karst.) seed stands from the Istebna region in the Beskidy Mountains of Poland. Significant differences were found among population in growth initiation, growth termination and the length of shoot elongation period. The two latter traits were highly correlated and were attributed to the altitude of the maternal stand. The tested populations were grouped based on phenology. Progeny of lower-alttitude stands ceased growth later, had a longer duration of shoot growth and greater current leader length compared to progeny of higher-altitude stands. Individual populations, however, did not differ in current-year growth and total tree height, implying higher within-population variation in growth traits than in phenology. The similar growth capacity of all tested populations suggests considerable gene flow between maternal stands, although differences in phenology imply the adaptation of progenies to the altitudinal environment of seed origin. These results suggest that within tested populations, selection is possible based on phenology alone, without considerable reduction of early height growth; final decisions, however, should be based on the environmental conditions of the planting site.  相似文献   

Crystallinity of wood and the size of cellulose crystallites in Norway spruce (<Emphasis Type="Italic">Picea abies</Emphasis>)   总被引：5，自引：0，他引：5  

Seppo?Andersson  Ritva?SerimaaEmail author  Timo?Paakkari  Pekka?Saranp??  Erkki?Pesonen 《Journal of Wood Science》2003,49(6):531-537

X-ray diffraction was used to study variations in the crystallinity of wood and the average thickness and length of the crystallites of cellulose as a function of the number of the year ring in Norway spruce [Picea abies (L.) Karst.]. The crystallinity increased from ring 4 to ring 10 from the pith and was constant after ring 10. The crystallinity of mature wood was about 30% ± 5%. The average thickness and average length of the crystallites were 3.2 ± 0.1nm and 28 ± 2nm, respectively; and no systematic variation of these values with the number of the year ring was observed. The mean microfibril angle decreased near the pith but was constant in the mature wood.  相似文献   

危害云杉的卷蛾三新种   总被引：1，自引：0，他引：1       下载免费PDF全文

刘友樵 《林业科学研究》1990,3(2):137-141

青海云杉是甘肃省祁连山林区的主要树种，曾遭受多种害虫侵害，经作者研究发现其中有卷蛾三新种，本文对它们进行比较详尽的新种记述。  相似文献   

青海三江源区青海云杉育苗密度与经济效益初探     

周国荣 《中南林业调查规划》2007,26(3):62-64

通过对三江源自然保护区青海云杉在不同育苗密度下生长状况的试验调查,以及经济效益的分析,结果表明:3 a生原床苗在密度为600万株/hm2时生长状况最佳,密度为900万株/hm2时经济效益最佳;6 a生移植苗在密度为180万株/hm2时生长状况最佳,密度为240万株/hm2时经济效益最佳。  相似文献   

Fine roots refilling process in an artificial gap in a Picea mongolica forest     

Zou Chun-jing  Ma Yong-liang  Zhang Chao  Xu Wen-duo 《中国林学(英文版)》2007,9(1):19-26

Picea mongolica is an endemic but endangered species in China. The spruce forest is only found in sandy forest-steppe ecotones. In this study, we examined the initial response of the quantity and refilling process of fine roots in an artificial canopy gap with a diameter of 36 m in a P. mongolica forest. Under the canopy, the fine root length densities of trees, shrubs and herbs were 2,622, 864 and 3,086 m·m–2, respectively. The fine root biomass of trees, shrubs and herbs were 148, 62 and 65 g·m–2, respect...  相似文献