热激处理对桃,李离体花枝2n花粉产生的影响   总被引：14，自引：1，他引：13  

张新忠  刘国俭 《园艺学报》1998,25(3):292-293

对桃和李通过休眠的花枝在２０℃条件下进行水培,减数分裂前期Ⅰ进行３２℃、１２ｈ热激处理,小孢子母细胞减数分裂四分体时期二分体发生频率为５．８２％～１６．０６％,２ｎ花粉比例为１３．３３％～２０．３７％,处理后花粉离体萌发率较低;３０℃恒温培养导致花蕾败育。二分体频率重复间、花朵间和品种间差异显著。  相似文献   

Increase of in vitro regeneration in Malus domestica by the application of phosphatase inhibitors   总被引：1，自引：0，他引：1  

C. Graph  G. Wricke 《Plant Breeding》1998,117(6):563-566

The effect of the phosphatase inhibitors sodium fluoride and sodium orthovanadaate on adventitious shoot regeneration from primary leaf explants was tested in eight genotypes of apple Malus domestica Borkh. Ten different concentrations between 0.5 and 100μM of each substance were added to a modified Murashige and Skoog regeneration medium containing 0.9 μM TDZ and 0.5 μM IBA. In general, all the con-centrations tested were able to promote shoot formation, although distinct maxima could be observed depending upon the genotype and the inhibitor. Shoot yield was influenced mostly by the genotype and by the concentration of the inhibitor, as well as the interaction between these two factors. In six of the eight genotypes the total number of regenerated shoots increased significantly compared with the control. This was achieved by an increase of the number of regenerating explants as well as the number of shoots per regenerating explant Comparing these results with those of former publications, application of the two phosphatase inhibitors resulted in an improved regeneration response in the corresponding genotypes. This method may also contribute to the understanding of in vitro regeneration in plants.  相似文献   

长汀县山麻鸭寄生蠕虫调查及其群落生态分析     

黄永康  王寿昆 《福建畜牧兽医》1997,(5):1-2

剖检长汀县２１羽山麻鸭，检得寄生蠕虫１０种，总感染率为８１％，平均感染强度和密度分别为３６条和３０条。各种蠕虫及其感染分别为：楔形前殖吸虫２８．６％，大多形棘头虫４．％，似椎低颈吸虫４．８％，福建单睾绦虫３３．３￥，美国膜壳绦虫４．８％。  相似文献   

杏大小孢子与雌雄配子体发育   总被引：7，自引：1，他引：6  

胡宝忠  李桂琴 《东北农业大学学报》1997,28(2):179-185

以龙垦２号品种为试材、分期取样固定，按常规石蜡法制片观察。结果表明，哈尔滨地区３月５日花药壁已分化，次生造孢细胞和花粉母细胞已形成。四分体属同时型，其排列方式为四面体型，成熟花粉呈椭圆形、具３孔沟。有的药室内产生巨型细胞。子房内具２胚珠，１枚后期退化消失。胚珠倒生，近珠孔处为双被，其余部分愈合；厚珠心，具珠心帽结构；胚囊发育属蓼型。杏雌蕊发育的异常情况较其它果树的比率都大。  相似文献   

CaCl_2处理对奈李低温贮藏期生理生化的影响     

苏冬梅  谷文众  陈灿  邓江望 《中南林业科技大学学报(自然科学版)》2001,(2)

为了探明 Ca2 对奈李低温贮藏的保鲜效应 ,对奈李采用不同浓度的 Ca Cl2 溶液处理、聚乙烯薄膜袋包装、在低温 ( 5℃ )下贮藏 ,研究其生理生化变化情况 .结果表明 :采用质量分数为 0 .5 %～ 1.0 %的 Ca Cl2 溶液处理 ,对奈李的呼吸作用、Vc含量的降低、可溶性固性物的消耗、电导率和 POD(过氧化物酶 )活性的升高以及贮藏期的失水等均具有抑制作用 ,而用较高浓度的 Ca Cl2 ( 2 .0 %～ 6.0 % )溶液处理 ,则具有相反的作用 .  相似文献   

Genetic Relationships among Prunus mume var. pendula Using AFLP Markers     

MING Jun  Zhang Qixiang  Ru Guangxin  Mao Qingshan  Yan Xiaolan  Lan Yanping 《中国林学(英文版)》2003,5(4)

Genetic relationships among Prunus mume var. pendula were studied by using AFLP markers. 18 accessions representing 14 cultivars ofPrunus murne var. pendula were selected from the germplasm collection at the Research Center of China Mci Flower. Seven Mse I-EcoR I AFLP primer combinations revealed 450 legible bands, and 269 of which were polymorphic markers. A similarity matrix was prepared using the simple matching coefficient of similarity and Nei‘s (72) distance coefficient. A UPGMA dendrogram demonstrated the genetic relationships of the cultivars. The information given by AFLP markers was basically consistent with the morphological classification and the evolutionary history of the morphotypes, and roughly supported the new revised classification system for Chinese Mci Cultivars. But there were still several exceptions: 1) the ‘Guhong Chuizhi‘ inserted between the ‘Tiaoxue Chuizhi‘ and the ‘Danfen Chuizhi‘; 2) the ‘Wufu Chuizhi‘ kept off the Pink Pendant Form, and the ‘Moshan Chuizhi‘ was removed from Viridiflora Pendant Form; 3) the ‘Danbi Chuizhi‘ and the ‘Shuangbi Chuizhi‘ of Viridiflora Pendant Form got together well but fell within the Pink Pendant Form.  相似文献   

栽培山杏是发展我市乡村经济的有效途径     

张亚梅  姜宝林  刘延海 《吉林林业科技》2001,30(6):41-42

山杏具有较高的利用价值和经济效益，而且适合我市栽培，大面积栽培山杏，是我市发展乡村经济的有效途径。  相似文献   

Influence of different forest conversion strategies on ground vegetation and tree regeneration in pine (<Emphasis Type="Italic">Pinus sylvestris</Emphasis> L.) stands: a case study in NE Germany     

Stefan Zerbe  Daria Kreyer 《European Journal of Forest Research》2007,126(2):291-301

The conversion of anthropogenic into more natural, self-regenerating forests is one of the major objectives of forestry throughout Europe. In this study, we present investigations on permanent plots with different silvicultural treatment in NE German pine stands. Management of old-growth pine stands on acidic and nutrient-poor sandy sites differs in fencing, thinning, and planting of certain tree species. The investigations were carried out on the community, population, and individual level of the pine forest ecosystems. Thus, vegetation changes, size and height of tree populations, and height increment of tree individuals were observed over a time span of 6 years. Special attention was paid to short-lived tree species such as, e.g., Frangula alnus and Sorbus aucuparia, as well as to Fagus sylvatica as one of the most typical forest tree species of Central Europe. Vegetation changes are interpreted as a consequence of natural regeneration of formerly degraded forest sites, involving an increase in nutrient availability. High browsing pressure can be considered as a key factor for the inhibition of tree seedlings and growth of saplings. Some Sorbus aucuparia individuals, however, succeeded in growing out of the browsing height also in unfenced stands. Few found specimens of Fagus sylvatica proved that this species is able to establish spontaneously on these relatively dry, acidic sites under continental climate influence. Such natural regeneration processes, also including spontaneous rejuvenation of trees, can be integrated into silviculture as passive forest conversion management. An active management like thinning of stands, planting of trees, and fencing can accelerate forest conversion with regard to height growth and species number of trees.  相似文献   

朝阳地区山杏花期及结实率影响因子的初步研究     

王洪江 《辽宁林业科技》2015,(3):18-19,54

山杏是辽宁朝阳地区主要的经济林树种,但结实率低。为解决这一问题,选取不同坡向、坡位(东北坡、东坡下腹、北坡、东坡上腹和南坡)的山杏林分,调查开花物候期和坐果率,研究不同气象因子对山杏坐果率的影响。结果表明:阳坡开花期最早,半阴坡略延迟,阴坡最晚。受温度、光照、相对湿度及风速影响,不同坡向的坐果率差异较大,即东北坡东坡下腹北坡东坡上腹南坡,同一坡向(东坡)的下腹要高于上腹。  相似文献   

三华李重要病害虫防治技术     

肖宁  周雅婷  李翌菡  柯沛强  邱宝利 《广东林业科技》2015,31(5):102-105

近年来随着广东省三华李（Prunus sp.）种植面积不断增加,三华李果园病虫害的危害上升趋势,对三华李水果的质量和产量影响较大。文章主要介绍了近年来三华李果园危害较为严重的几种病虫害的发生、危害情况以及主要防治技术。  相似文献