用RAPD和SSR分子标记鉴定小金海棠F1代 杂种实生苗的研究   总被引：5，自引：0，他引：5  

唐建民  周世良  成明昊  林启冰  周志钦 《中国农学通报》2006,22(2):36-36

小金海棠（Malus xiaojinensis）具有多种抗逆特性，是苹果属植物中一种珍贵的野生种质资源。由于小金海棠具有无融合生殖特性，早期准确、快速、有效地鉴定其F1代杂种实生苗对于研究利用其众多抗逆基因具有重要意义。RAPD技术是现有鉴定果树杂种实生苗最为简单、快速和有效的分子标记技术，但RAPD技术本身存在一定的不稳定性。笔者对小金海棠和山荆子（M. baccata）F1代杂交实生苗79个单株进行了RAPD分析，并用SSR标记对RAPD标记的初步鉴定结果进行了进一步的验证。结果显示，RAPD标记鉴定出的杂种实生苗有72.2%得到SSR标记的证实。研究的结果还表明，“叠加”和“新带”两种RAPD带型，结合RAPD标记特异位点数可以为小金海棠F1代杂种实生苗提供可靠的鉴定依据。研究为利用RAPD技术早期快速鉴定果树杂种实生苗提供了新的信息和依据。  相似文献   

利用SSR标记鉴定桂单22号杂交一代种子纯度的研究   总被引：3，自引：1，他引：3  

吴永升  谭华  郑德波  陈国品  刘丕庆  黄开健  杨晓杰 《种子》2006,25(3):28-30

以桂单22号玉米杂交种及其亲本和10对玉米SSR位点引物为材料，筛选出了适合该杂交种纯度鉴定的SSR引物；并对利用嫩芽和干种子为材料提取DNA的效果进行了比较，建立了一套利用SSR标记技术快速，简便进行玉米杂交种纯度鉴定的技术规程。  相似文献   

Analysis of wheat-Thinopyrum intermedium derivatives with BYDV-resistance     

Zhi-Shan Lin  Zhi-Fu Cui  Xiang-Yan Zeng  You-Zhi Ma  Zeng-Yan Zhang  Toshiki Nakamura  Goro Ishikawa  Kazuhiro Nakamura  Hisashi Yoshida  Zhi-Yong Xin 《Euphytica》2007,158(1-2):109-118

Chromosome compositions of seven lines, derived from hybrids between a wheat cultivar and the wheat-Thinopyrum intermedium addition line Z6, with barley yellow dwarf virus (BYDV) resistance, were determined by genomic in situ hybridization, cytogenetic and SSR assays. The results showed that line N522 was a disomic addition line, lines N420 and N439 were 2Ai-2(2B) chromosome substitution lines, lines N431 and N452 were 2Ai-2(2D) chromosome substitution lines, line N523 was a 2Ai-2S(2D) ditelosomic substitution line, and line N530 was a double ditelosomic line with the mitotic chromosome number of 2n = 40 + 4t. One pair of telosomes in line N530 lacked several proximal SSR markers of chromosome 2AS, but possessed certain terminal markers, which were consistent with an acrocentric structure, and the other pair of chromosome arms were presumably 2Ai-2S telosomes with BYDV-resistance. These wheat-Th. intermedium lines provide useful genetic resources for developing alien chromosome translocation lines.  相似文献   

Variation of the genomic proportion of the recurrent parent in BC₁ and its relation to yield performance in sorghum (Sorghum bicolor) breeding for low-input conditions     

R. Uptmoor    W. Wenzel    K. Ayisi    G. Donaldson    A. Gehringer    W. Friedt    F. Ordon 《Plant Breeding》2006,125(5):532-534

In order to define the variation of the genomic proportion of the recurrent parent [G(RP)] and its relation to yield, G(RP) of individual BC₁ plants of two sorghum populations composed of a high‐yielding cultivar as recurrent parent (RP) and a donor with superior drought resistance or grain quality, respectively, was estimated using AFLPs and SSRs. G(RP) in BC₁ ranged from 0.53 to 0.95 and averaged to 0.76 in the population (NP4453 × ‘SV‐2’) × ‘SV‐2’. G(RP) varied between 0.60 and 0.86 and averaged to 0.74 in the BC₁ of (ICV‐219 × ‘SV‐2’) × ‘SV‐2’. Results show that plants with a G(RP) equivalent to BC₂ (0.875) or BC₃ (0.938), respectively, can be selected from BC₁. Yield performance of BC₁S₁ families was tested in field trials carried out in South Africa. The correlation between yield and G(RP) in BC₁ was low. Selection according to G(RP) did not result in an effective preselection for yield.  相似文献   

Identification of QTLs for rice grain size and shape of Iranian cultivars using SSR markers   总被引：6，自引：0，他引：6  

B. Rabiei  M. Valizadeh  B. Ghareyazie  M. Moghaddam  A.J. Ali 《Euphytica》2004,135(3):325-332

Grain size is a main component of rice appearance quality. In this study, we performed the SSR mapping of quantitative trait loci (QTLs) controlling grain size (grain length and breadth) and shape (length/breadth ratio) using an F₂ population of a cross between two Iranian cultivars, Domsephid and Gerdeh, comprising of 192 individuals. A linkage map with 88 markers was constructed, which covered 1367.9 cM of the rice genome with an average distance of 18 cM between markers. Interval mapping procedure was used to identify the QTLs controlling three grain traits, and QTLs detected were further confirmed using composite interval mapping. A total of 11 intervals carrying 18 QTLs for three traits were identifed, that included five QTLs for grain length, seven QTLs for grain breadth, and six QTLs for grain shape. A major QTL for grain length was detected on chromosome 3, that explained 19.3% of the phenotypic variation. Two major QTLs for grain breadth were mapped on chromosomes 3 and 8, which explained 34.1% and 20% of the phenotypic variation, respectively. Another two major QTLs were identified for grain shape on chromosomes 3 and 8, which accounted for 27.1% and 20.5% of the phenotypic variance, respectively. The two QTLs that were mapped for grain shape coincided with the major QTLs detected for grain length and grain breadth. Intrestingly, gs2 QTL specific to grain shape was detected on chromosome 2 that explained 15% of the phenotypic variation.  相似文献   

Segregation distortion in doubled haploid lines of barley (Hordeum vulgare L.) detected by simple sequence repeat (SSR) markers     

Haitham Sayed  Hamed Kayyal  Luke Ramsey  Salvatore Ceccarelli  Michael Baum 《Euphytica》2002,125(2):265-272

A total of 147 simple sequence repeat (SSR) markers (including86 barley and 61 wheat microsatellite markers) were tested for their segregation in a doubled haploid (DH) and an F₂ population of barley. The DH population consisted of 71 doubled haploid lines, developed from F₁ plants of a cross between Tadmor and WI2291using isolated microspore culture technique. A genetic linkage map consisting of 43 microsatellite markers was constructed using the DH population. Particularly on chromosome 4H microsatellite markers showed distorted segregation ratios. Segregation of DH lines based on molecular markers were compared with segregation of 92 F₂ lines from the same cross. The proportion of loci deviating from the expected monogenic segregation ratios in the DH population was significantly higher (19/43loci, 44%) than in the F₂ population (7/43 loci, 16%). The deviation was biased towards the WI2291 parent alleles. In line with this observation, WI2291 was found to perform better than Tadmor in regenerating green plantlets with the isolated microspore-culture technique. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

利用若干单株混合提取DNA方法进行玉米群体SSR的分析   总被引：9，自引：1，他引：9  

王铁固  陈彦惠  吴连成  库丽霞  侯本军 《华北农学报》2005,20(1):6-11

以金皇后玉米群体90个单株：DNA和6个自交系DNA为供试材料，利用15对SSR引物，比较了同一群体4种DNA样品处理(单株DNA样品、3个单株混合DNA样品、10个单株混合DNA样品、15个单株混合DNA样品)的SSR遗传多态性。结果表明，利用单株DNA样品能获得等位基因数、基因频率、基因型杂合度等较全面的遗传信息，适宜进行单个或少量群体的遗传结构研究，但试验工作量大；利用混合DNA样品所能获得的遗传信息有所减少，比较4种DNA样品处理的检测结果发现，15个单株混合样品的检测结果误差较大，而采用3～10个单株DNA混合的样品基本可以反映出一个群体的等位基因数目和SSR带型的差异，由于试验工作量大大减少，可以应用于较多玉米群体间遗传差异的比较。  相似文献   

水稻PCD相关基因的定位研究   总被引：1，自引：0，他引：1  

李喜焕  刘国振  刘国庆  朱立煌  马峙英 《作物学报》2003,29(6):942-946

植物中的细胞程序化死亡(PCD)在植物的发育、抗病及植物与环境互作等过程中发挥着极其重要的作用.本研究以我国学者发现的特殊的水稻细胞死亡控制突变体3037(M)为材料, 采用微卫星DNA标记 (共计159个微卫星引物) 技术对突变体性状进行基因定位研究.结果表明, 该突变体PCD基因位于水稻的第8染色体和第12染色体,为下一步精细定  相似文献   

棉花表型性状基因的SSR标记定位   总被引：8，自引：0，他引：8  

倪西源  王学德  孙志栋 《棉花学报》2003,15(6):357-360

本文以两个陆地棉多标记基因系T582和T586,以及杂交获得的F_1、F_2及F_3代作为试验材料,利用11对SSR引物对F_2群体的120个单株的DNA样品进行多态性分析,并利用F_2和F_3群体对F_2群体对应单株的13个表型性状进行基因型的判定,结果得到了3个与表型性状基因连锁的SSR标记,分别是红茎基因(R_1)与J178连锁、遗传距离为24.9cM,簇生铃基因(CL_1)与J236连锁,遗传距离为46.0cM,茸毛基因(T_1)与J252连锁,遗传距离为28.5cM,其中R_1、CL_1、J178和J236在同一连锁群上。红茎和植株茸毛是具有抗虫性能的形态性状,用SSR标记这些性状将有助于提高育种家的育种效率。  相似文献   

水稻早世代稳定特异性的研究   总被引：8，自引：0，他引：8  

周黎军  吴先军  李仕贵 《作物学报》2005,31(6):677-685

利用9个具有早世代稳定遗传特性的水稻品系和7个栽培稻作为主体亲本杂交配组，在130个组合F2群体963个株系中，发现28个组合中出现73个稳定株系。遗传分析表明水稻早世代稳定既不是质量性状遗传也不是数量性状遗传，是按株群分离的遗传方式，出现稳定株系的组合F1群体发生了分离，F2群体中既有性状不分离的稳定株系又有孟德尔  相似文献