Effects of Bacillus amylolyticus on Rumen Dry Matter Digestibility,Fermentation Parameters and Metabolites of Dairy Cows北大核心CSCD     

李玉琴黄新高鹏翔赵小博王慧蒋林树 《动物营养学报》2023,(1):311-322

This experiment was conducted to study the effects of Bacillus amylolyticus on rumen dry matter digestibility, fermentation parameters and metabolites of dairy cows. Experiment 1: six dairy cows with permanent fistula and had the similar parity (2 to 3 fetuses), body weight [(662 ±57) kg], lactation days [(160 ± 22) d] and milk yield [(36.1 ±3.8) kg / d], were randomly divided into 3 groups with 2 cows per group. Using 3 ×3 Latin square design, each group was extra fed 0 (control group), 5 ×109 and 5 ×1010 CFU / d Bacillus amylolyticus, the trial period was 3 periods, 13 days for each period, including 10 days for the pre-trial period and 3 days for the trial period. At the beginning of the trial period, the rumen dry matter digestibility of cows was measured by nylon bag method. Experiment 2: thirty dairy cows with similar parity [(2.5 ±0.3) fetuses], initial body weight [(559.2 ± 7. 4) kg], milk yield [(35. 2 ± 1. 5) kg / d] and lactation days [(99 ± 22) d] were randomly divided into three groups with 10 cows in each group. Cows in the 3 groups were extra fed with 0 (control group), 5 ×109 and 5 ×1010 CFU / d Bacillus amylolyticus, respectively. The pre-trial period was 10 days and the trial period was 42 days. The rumen fluid was collected on the last day of the trial period, and the rumen fermentation parameters and metabolites were determined. The results showed as follows: compared with the control group, 1) supplementary feeding of 5 × 1010 CFU / d Bacillus amylolyticus could significantly improve the rumen dry matter digestibility of dairy cows at 12, 24, 36, 48 and 72 h (P <0.05), supplementary feeding of 5 ×109 CFU / d Bacillus amylolyticus could significantly improve the rumen dry matter digestibility of dairy cows at 36 and 48 h (P <0.05); 2) supplementary feeding of 5 ×109 CFU / d Bacillus amylolyticus could significantly increase the contents of isobutyric acid, butyric acid, isovaleric acid, valeric acid, total volatile fatty acid and microbial protein in the rumen of dairy cows (P < 0. 05), supplementary feeding of 5 × 1010 CFU / d Bacillus amylolyticus could significantly increase the contents of isobutyric acid and isovaleric acid in the rumen of dairy cows and significantly reduce the rumen acetic acid / propionic acid (P < 0. 05), supplementary feeding of 5 ×109 and 5 ×1010 Bacillus amylolyticus had no effects on rumen pH and ammonia nitrogen content (P >0.05); 3) eleven metabolic pathways related to differential metabolites were detected, and ascorbic acid and alginate metabolism were significantly enriched (P < 0. 05) . In conclusion, Bacillus amylolyticus can increase the rumen dry matter digestibility, and improve the rumen fermentation function of dairy cows by affecting the content of rumen metabolites. By comprehensive analysis, supplementary feeding of 5 × 109 CFU / d Bacillus amylolyticus can achieve better effect. © 2023 Authors. All rights reserved.  相似文献   

Effects of Compound Plant Nutrients on Rumen Fermentation Parameters,Rumen Microbiota and Fatty Acid Composition of Longissimus dorsi Muscle in Finishing Small Tail Han Sheep北大核心CSCD     

王园  王文文  杨毅  高畅  郭焘  段婷  刘娜  安晓萍  齐景伟 《动物营养学报》2023,(1):380-390

This study was conducted to investigate the effects of compound p1ant nutrients (CPN) on rumen fermentation parameters, rumen microbiota and fatty acid composition of longissimus dorsi musc1e in finishing sma11 tai1 Han sheep. Sixteen 4-month-o1d finishing sma11 tai1 Han sheep with an initia1 body weight (BW) of (24.18±0.31) kg were random1y divided into two groups, name1y, with 8 rep1icates per group and 1 sheep per rep1icate. The sheep in the contro1 group were fed a basa1 diet, whereas the sheep of the contro1 group (CPN group) was fed the basa1 diet supp1ementation with 3‰ CPN. The experiment 1asted for 97 days after 7 days adaption. The resu1ts showed as fo11ows: compared with the contro1 group, 1) adding CPN decreased the concentration of ammonia nitrogen (NH3-N) (P<0.05); 2) CPN supp1ementation affected beta diversity of rumen microbiota; 3) the re1ative abundance of Acidobacteriota, Erysipe1otrichaceae_UCG-002, Lactobacillus, and Megasphaera were enhanced by adding CPN (P<0.05), whereas, the re1ative abundance of Bacteroidetes and Prevotella was 1ower (P<0.05); 4) the supp1ementation of CPN had no significant effect on fatty acid composition of longissimus dorsi musc1e of fattening sma11-tai1ed Han sheep (P>0.05); 5) the contents of tota1 MUFA, C18:1n9c, C14:1, C16:1, C18:2n6c, C18:3n3 and n-3PUFA in the longissimus dorsi musc1e were corre1ated with the re1ative abundance of Megasphaera, Erysipe1otrichaceae_UCG-002, Succiniclasticum and Ruminococcus (P<0.05). In conc1usion, CPN can regu1ate the rumen microbiota structure and reduce the rumen NH3-N concentration of fattening sma11-tai1ed Han sheep. In production practice, CPN can be used as a rumen eco1ogica1 regu1ator. © 2023 Authors. All rights reserved.  相似文献   

不同粒度猫尾草对羔羊体外发酵特性和微生物数量的影响     

王静  孔令莹  徐建风  康静  沈振峰  刘婷 《草业学报》2023,32(3):224-233

通过Rusitec-s体外发酵系统研究不同粒度猫尾草对羔羊体外发酵参数与微生物数量的影响,选取6种不同粒度(1.00、2.36、3.35、4.75、8.00和12.50 mm)的猫尾草作为试验饲粮的纤维来源,按照猫尾草的粒度将试验分为6个处理组,每个处理4个重复,利用Rusitec-s型人工瘤胃模拟装置进行48 h体外发酵,测定养分降解率、发酵参数及发酵液中6种微生物菌群的拷贝数。结果表明：与其他处理组相比,2.36 mm组显著提高了体外中性洗涤纤维降解率(IVNDFD)、丁酸、异丁酸、戊酸、异戊酸摩尔比和溶纤维丁酸弧菌(B. f)数量(P<0.05),且显著降低总产甲烷菌(TMe)数量(P<0.05)。总菌(TB)与氨态氮(NH3-N)浓度、乙酸摩尔比呈正相关(R²=0.84,P=0.038;R²=0.88,P=0.021);B. f数量与丙酸摩尔比呈正相关(R²=0.82,P=0.045),且与pH呈负相关(R²=-0.98,P=0.001);TMe数量与体外粗蛋白质降解率(IVCP...  相似文献   

生物质能源作物——能源甜菜的开发利用     

史淑芝  程大友  马凤鸣  崔杰 《中国农学通报》2007,23(11):416-419

随着全世界能源物质的不断扩展,可再生的生物质能源将是21世纪主要的能源物质。能源甜菜是新兴的可再生能源作物,它具有生物学产量高,乙醇转化率高,含糖量适中等特点,是取之不尽、用之不竭的环保能源,对环境几乎没有污染;同时又是糖饲兼收的作物。利用能源甜菜通过酒精发酵创造乙醇燃料新能源,可以减轻或消除与粮食争地的矛盾,是保证粮食安全和能源安全的双赢举措。  相似文献   

高效产氢发酵细菌在不同气相条件下产氢   总被引：12，自引：0，他引：12  

林明  任南琪  王爱杰  马放 《中国沼气》2002,20(2):3-7,23

气相条件是影响高效产氢细菌产氢能力的重要生态因子之。间歇实验结果表明，空气中的氧分子是抑制高效产氢细菌－B49生长发酵的直接原因，二氧化碳也对B49的发酵产氢产生很大的抑制作用，而以氮气和氩气为气相能使B49发挥较高的产氢能力，氮气很可能对B49的发酵产氢有一定的促进作用。  相似文献   

有机废水产酸发酵的生理生态学分析   总被引：9，自引：0，他引：9  

任南琪  王宝贞 《中国沼气》1995,13(1):1-6

通过对产酸发酵细菌细胞内生物物理化学及发酵途径分析表明，控制生物产酸发酵类型的主要因素有ＮＡＤＨ／ＮＡＤ＋平衡和发酵产物酸性末端数量。根据微生物的生理生态学原理及试验结果分析，证实所发现的乙醇型发酵优于丁酸型发酵和丙酸型发酵。此外，还对氢分压与丙酸积累的相关性提出新的见解。  相似文献   

奶牛养殖场粪污处理沼气工程技术与模式   总被引：9，自引：3，他引：9  

胡启春  宋立 《中国沼气》2005,23(4):22-25

本文综述了国内外奶牛养殖场沼气工程技术应用现状,分析了奶牛粪沼气发酵潜力和三种主要沼气发酵工艺的技术特点.  相似文献   

回转筒内秸秆碎料回转周期和料层完全混合时间研究     

周敬之  周知星  冯俊小  李十中 《农业机械学报》2015,46(8):178-185,238

基于试验和理论分析,研究了回转筒型固态发酵罐内秸秆粉碎料的料床回转周期,建立了数学模型,其计算准确度达95%以上;结合图像分析法和料床回转周期,发现筒内上下料层完全混合时料层所转圈数与筒体转速关系不大,与筒内料体积填充率呈线性关系。由此回归并推导出只需知道筒内料体积填充率和筒体转速,便可预测上下料层完全混合的公式,其计算准确度达90%以上。提出了以上研究成果在工业级发酵罐中应用的方法。  相似文献   

固体物理教学改革的探索     

刘思平 《湖南农机》2007,(5):74-75

本文从固体物理学在高等教育中的重要性和目前的教学现状出发,对固体物理教学内容、方法、手段等方面的改革进行了一些探索,以期固体物理的教学能紧跟发展的步伐。  相似文献   

铁法预处理糖蜜酒精废液沼气发酵工艺的研究   总被引：4，自引：1，他引：4  

符征鸽  赵跃新 《中国沼气》1995,13(3):10-13

本研究采用法法预处理糖蜜精废液，并将其进行厌氧发酵试验，试验的厌氧罐容积为１３Ｌ，预处理容积为１．３Ｌ结果表明：经铁法预处理后的糖蜜酒精度液能够稳定地用于沼气发酵，而且所产沼气的Ｈ２Ｓ含量大减少。  相似文献