Contamination and browning in tissue culture of Platanus occidentalis L.     

Tao Feng-jie  Zhang Zhi-yi  Zhou Jun  Yao Na  Wang Dong-mei 《中国林学(英文版)》2007,9(4):279-282

Twigs of 2–3-year-old Platanus occidentalis L. were used as experimental material to find the causes for the contamination and browning in the initial stages of tissue cultures. To compare the degree of browning of explants picked off from different growing seasons, the experimental material was excised from trees on each of the first ten days in January, March, May and July, 2006. The results indicated that the contamination and browning rates of the material cut off in January (14.2% and 30.6%, respectively) and March were somewhat lower than those in July. The pretreatment of soaking the explants in different anti-oxidants and absorbents at the same time could diminish some side effects. The pretreatment of using 10 g·L–1 vitamin C reduced the contamination and brown-ing rate effectively. An orthogonal experiment showed that the optimal factor and level arrangement is 0.5 mg·L–1 BA, 2.0 g·L–1 ac-tive carbon and 1.5 g·L–1 PVP which resulted in a browning rate of only 16.5%. In general, sampling period, physical properties and pretreatment of explants are the main factors responsible for the contamination and browning of material in the initial stages of P. occidentalis tissue cultures.  相似文献   

应用正交设计进行矮牵牛组培苗生根条件的筛选     

吴林森  冯福娟 《福建林业科技》2006,33(3):44-46,64

采用正交设计方法对矮牵牛组培苗生根的条件进行筛选。试验表明1/2MS+BA 0.1 mg.L-1+IBA 0.8 mg.L-1+NAA 3.0 mg.L-1,蔗糖30 g.L-1的培养基对矮牵牛组培苗生根最有利。  相似文献   

降压草的组织培养技术研究     

郭福生  陈淑华  王漫  叶雅玲 《防护林科技》2015,(6)

以白城市林业科学研究院选育的新品种降压草2号为试材,利用其带芽茎段进行组织培养,筛选降压草组织培养程序及培养基配方。结果表明:适宜的腋芽诱导培养基为MS+6-BA0.5mg L-1+NAA0.1mg L-1,适宜的增殖培养基为MS+6-BA1.0mg L-1+NAA0.5mg L-1+IAA0.1mg L-1。  相似文献   

兽药广告宣传中的中西方文化差异分析     

李鹏飞 《中国兽药杂志》2015,49(12):61-64

介绍了中西方兽药广告宣传的各种表现形式,分析了中西方文化差异对兽药广告宣传的影响,提出了我国兽药广告宣传在融合中西方文化的同时,要多学习西方优秀作品的创意,使之相互融合、相互渗透的发展建议,从而推动我国优秀的兽药企业及兽药产品走向世界。  相似文献   

Suppression of influenza virus infection by the orf virus isolated in Taiwan     

Fong-Yuan LIN  Yeu-Yang TSENG  Kun-Wei CHAN  Shu-Ting KUO  Cheng-Hsiung YANG  Chi-Young WANG  Masaki TAKASU  Wei-Li HSU  Min-Liang WONG 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1055-1062

Orf virus (ORFV), a member of parapoxvirus, is an enveloped virus with genome of double-stranded DNA. ORFV causes contagious pustular dermatitis or contagious ecthyma in sheep and goats worldwide. In general, detection of viral DNA and observing ORFV virion in tissues of afflicted animals are two methods commonly used for diagnosis of orf infection; however, isolation of the ORFV in cell culture using virus-containing tissue as inoculum is known to be difficult. In this work, the ORFV (Hoping strain) isolated in central Taiwan was successfully grown in cell culture. We further examined the biochemical characteristic of our isolate, including viral genotyping, viral mRNA and protein expression. By electron microscopy, one unique form of viral particle from ORFV infected cellular lysate was demonstrated in the negative-stained field. Moreover, immunomodulating and anti-influenza virus properties of this ORFV were investigated. ORFV stimulated human monocytes (THP-1) secreting proinflammatory cytokines IL-8 and TNF-α. And, pre-treatment of ORFV-infected cell medium prevents A549 cells from subsequent type A influenza virus (IAV) infection. Similarly, mice infected with ORFV via both intramuscular and subcutaneous routes at two days prior to IAV infection significantly decreased the replication of IAV. In summary, the results of a current study indicated our Hoping strain harbors the immune modulator property; with such a bio-adjuvanticity, we further proved that pre-exposure of ORFV protects animals from subsequent IAV infection.  相似文献   

The gene expression pattern induced by high plating density in cultured bovine and buffalo granulosa cells might be regulated by specific miRNA species     

Vengala Rao YENUGANTI  Vijay Simha BADDELA  Anja BAUFELD  Dheer SINGH  Jens VANSELOW 《The Journal of reproduction and development》2015,61(2):154-160

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Evaluation of embryonic age and the effects of different proteases on the isolation and primary culture of chicken intestinal epithelial cells in vitro          下载免费PDF全文

Chao Yuan  Qiang He  Jun‐ming Li  Mahmoud Mostafa Azzam  Jian‐jun Lu  Xiao‐ting Zou 《Animal Science Journal》2015,86(6):588-594

The present study evaluates the effects of embryonic age and proteolytic enzymes on the isolation and primary culture of chicken enterocyte and to establish an effective technique for chicken intestinal epithelial cell (IEC) cultivation. Fourteen‐day‐old, 16‐day‐old and 18‐day‐old embryos (average weight: 52.23 ± 0.76 g, 50.86 ± 0.99 g, 48.98 ± 1.03 g) were the source for preparation of enterocyte culture, and trypsin‐ethylene diamine tetraacetic acid, collagenase, thermolysin and combination of collagenase and thermolysin were used for digestion medium. Optimal culture protocols were determined by qualitative assays of proliferation. Cells isolated by using 14‐day‐old embryo and collagenase obtain the best attachment and growth in culture, and the production of continuously growing IEC cultures. Thus, we conclude that the use of collagenase as a dissociating enzyme and 14‐day‐old embryo as a source can be advantageously applied to the isolation of chicken IEC and this method may be useful for various applications and basic studies of the intestinal tract concerning such objects as physiology, immunology and toxicology.  相似文献   

Comparison of the canine corneal epithelial cell sheets cultivated from limbal stem cells on canine amniotic membrane,atelocollagen gel,and temperature‐responsive culture dish          下载免费PDF全文

Eunryel Nam  Naoki Fujita  Maresuke Morita  Keiko Tsuzuki  Hsing Yi Lin  Cheng Shu Chung  Takayuki Nakagawa  Ryohei Nishimura 《Veterinary ophthalmology》2015,18(4):317-325

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竹子试管苗开花的初步研究   总被引：16，自引：3，他引：16  

张光楚  王裕霞 《竹子研究汇刊》2001,20(1):1-4

麻竹试管苗经3年在高浓度BA的培养基中培养，保存下来的16个无性系都出现开花现象，开花迟早跟无性系的繁芽能力有关，繁芽能力强的无性系较早开花，繁芽能力弱的无性系较迟开花。但这样诱导出来的花质量不好，不能用于授粉。当降低培养基中BA的用量，增加KT、NAA、椰乳，使花芽在营养比较丰富的培养基上培养，花的质量得到明显改善，但也伴随着出现书柜生殖生长向营养生长逆转的现象，逆转的程度与KT的用量有关，当KT0．5mg/l时只表现花枝增长，当KT1．25mg/l时，花枝上长出许多新的营养枝。这一现象表明细胞分裂素BA、KT在竹子组织培养过程中的作用不是完全相同的，BA有利于花芽分化，而KT则有利于营养生长的改善。  相似文献   

热激处理对甜柿外植体总酚含量和PAL、PPO、POD活性的影响     

杨谷良  范媛媛  邓先珍 《经济林研究》2015,(1)

甜柿在组织培养过程中极易褐化,为了防止褐变,在培养之前应对外植体进行热激处理。文中研究了热激处理后的宝华甜柿叶片在培养过程中其酚类物质含量和苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化物酶(POD)活性的变化情况,并对热激处理抑制组培褐变的生理机制进行了初步探讨。结果表明:热激处理后,宝华甜柿叶片的褐变程度比没有处理的明显减轻,其总酚含量与苯丙氨酸解氨酶(PAL)及多酚氧化酶(PPO)活性均有所降低,但热激处理对其过氧化物酶(POD)活性的抑制效果并不明显,说明热激处理通过降低外植体的PAL及PPO活性,抑制酚类物质的合成及氧化,减少了褐变产物——醌类物质的形成,从而有效抑制了褐变现象的发生。  相似文献