兽药广告宣传中的中西方文化差异分析     

李鹏飞 《中国兽药杂志》2015,49(12):61-64

介绍了中西方兽药广告宣传的各种表现形式,分析了中西方文化差异对兽药广告宣传的影响,提出了我国兽药广告宣传在融合中西方文化的同时,要多学习西方优秀作品的创意,使之相互融合、相互渗透的发展建议,从而推动我国优秀的兽药企业及兽药产品走向世界。  相似文献   

Suppression of influenza virus infection by the orf virus isolated in Taiwan     

Fong-Yuan LIN  Yeu-Yang TSENG  Kun-Wei CHAN  Shu-Ting KUO  Cheng-Hsiung YANG  Chi-Young WANG  Masaki TAKASU  Wei-Li HSU  Min-Liang WONG 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1055-1062

Orf virus (ORFV), a member of parapoxvirus, is an enveloped virus with genome of double-stranded DNA. ORFV causes contagious pustular dermatitis or contagious ecthyma in sheep and goats worldwide. In general, detection of viral DNA and observing ORFV virion in tissues of afflicted animals are two methods commonly used for diagnosis of orf infection; however, isolation of the ORFV in cell culture using virus-containing tissue as inoculum is known to be difficult. In this work, the ORFV (Hoping strain) isolated in central Taiwan was successfully grown in cell culture. We further examined the biochemical characteristic of our isolate, including viral genotyping, viral mRNA and protein expression. By electron microscopy, one unique form of viral particle from ORFV infected cellular lysate was demonstrated in the negative-stained field. Moreover, immunomodulating and anti-influenza virus properties of this ORFV were investigated. ORFV stimulated human monocytes (THP-1) secreting proinflammatory cytokines IL-8 and TNF-α. And, pre-treatment of ORFV-infected cell medium prevents A549 cells from subsequent type A influenza virus (IAV) infection. Similarly, mice infected with ORFV via both intramuscular and subcutaneous routes at two days prior to IAV infection significantly decreased the replication of IAV. In summary, the results of a current study indicated our Hoping strain harbors the immune modulator property; with such a bio-adjuvanticity, we further proved that pre-exposure of ORFV protects animals from subsequent IAV infection.  相似文献   

The gene expression pattern induced by high plating density in cultured bovine and buffalo granulosa cells might be regulated by specific miRNA species     

Vengala Rao YENUGANTI  Vijay Simha BADDELA  Anja BAUFELD  Dheer SINGH  Jens VANSELOW 《The Journal of reproduction and development》2015,61(2):154-160

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Evaluation of embryonic age and the effects of different proteases on the isolation and primary culture of chicken intestinal epithelial cells in vitro          下载免费PDF全文

Chao Yuan  Qiang He  Jun‐ming Li  Mahmoud Mostafa Azzam  Jian‐jun Lu  Xiao‐ting Zou 《Animal Science Journal》2015,86(6):588-594

The present study evaluates the effects of embryonic age and proteolytic enzymes on the isolation and primary culture of chicken enterocyte and to establish an effective technique for chicken intestinal epithelial cell (IEC) cultivation. Fourteen‐day‐old, 16‐day‐old and 18‐day‐old embryos (average weight: 52.23 ± 0.76 g, 50.86 ± 0.99 g, 48.98 ± 1.03 g) were the source for preparation of enterocyte culture, and trypsin‐ethylene diamine tetraacetic acid, collagenase, thermolysin and combination of collagenase and thermolysin were used for digestion medium. Optimal culture protocols were determined by qualitative assays of proliferation. Cells isolated by using 14‐day‐old embryo and collagenase obtain the best attachment and growth in culture, and the production of continuously growing IEC cultures. Thus, we conclude that the use of collagenase as a dissociating enzyme and 14‐day‐old embryo as a source can be advantageously applied to the isolation of chicken IEC and this method may be useful for various applications and basic studies of the intestinal tract concerning such objects as physiology, immunology and toxicology.  相似文献   

Comparison of the canine corneal epithelial cell sheets cultivated from limbal stem cells on canine amniotic membrane,atelocollagen gel,and temperature‐responsive culture dish          下载免费PDF全文

Eunryel Nam  Naoki Fujita  Maresuke Morita  Keiko Tsuzuki  Hsing Yi Lin  Cheng Shu Chung  Takayuki Nakagawa  Ryohei Nishimura 《Veterinary ophthalmology》2015,18(4):317-325

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竹子试管苗开花的初步研究   总被引：16，自引：3，他引：16  

张光楚  王裕霞 《竹子研究汇刊》2001,20(1):1-4

麻竹试管苗经3年在高浓度BA的培养基中培养，保存下来的16个无性系都出现开花现象，开花迟早跟无性系的繁芽能力有关，繁芽能力强的无性系较早开花，繁芽能力弱的无性系较迟开花。但这样诱导出来的花质量不好，不能用于授粉。当降低培养基中BA的用量，增加KT、NAA、椰乳，使花芽在营养比较丰富的培养基上培养，花的质量得到明显改善，但也伴随着出现书柜生殖生长向营养生长逆转的现象，逆转的程度与KT的用量有关，当KT0．5mg/l时只表现花枝增长，当KT1．25mg/l时，花枝上长出许多新的营养枝。这一现象表明细胞分裂素BA、KT在竹子组织培养过程中的作用不是完全相同的，BA有利于花芽分化，而KT则有利于营养生长的改善。  相似文献   

热激处理对甜柿外植体总酚含量和PAL、PPO、POD活性的影响     

杨谷良  范媛媛  邓先珍 《经济林研究》2015,(1)

甜柿在组织培养过程中极易褐化,为了防止褐变,在培养之前应对外植体进行热激处理。文中研究了热激处理后的宝华甜柿叶片在培养过程中其酚类物质含量和苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化物酶(POD)活性的变化情况,并对热激处理抑制组培褐变的生理机制进行了初步探讨。结果表明:热激处理后,宝华甜柿叶片的褐变程度比没有处理的明显减轻,其总酚含量与苯丙氨酸解氨酶(PAL)及多酚氧化酶(PPO)活性均有所降低,但热激处理对其过氧化物酶(POD)活性的抑制效果并不明显,说明热激处理通过降低外植体的PAL及PPO活性,抑制酚类物质的合成及氧化,减少了褐变产物——醌类物质的形成,从而有效抑制了褐变现象的发生。  相似文献   

生态景观林带在城乡宜居建设中的作用     

苏晨辉  刘碧云  陆康英 《中南林业调查规划》2015,(2)

面对伴随着城市现代化进程所带来的环境问题,宜居城乡建设是改善居住环境问题的重要举措;生态景观林带是以改善破碎化的森林斑块和绿化带、以增强森林为主体的自然生态空间的连通性、以观赏性为目的和以点、线、面相结合的景观廊道网络。生态景观林带在城乡宜居建设中发挥着改善自然环境与人文环境的双重作用,促进了城市与乡村的耦合,是城乡宜居建设的有效途径。  相似文献   

“0.618黄金杯”和不同基质对油茶组培苗生根的影响     

蔡冬元 《经济林研究》2015,(1)

为了解决油茶组培苗根系发生困难的问题,将油茶无菌短枝组培苗分别扦插在运用"0.618黄金水位"原理制作的"黄金杯"和扦插常用的传统营养杯中,两种营养杯中分别填入以1︰1的珍珠岩与草炭的混配基质、细河沙及红心土,就不同营养杯和不同基质处理对油茶组培苗生根的影响情况进行了对比试验。结果表明:采用1︰1的珍珠岩与草炭的混配基质,将油茶无菌短枝组培苗扦插在运用"0.618黄金水位"原理制作的"黄金杯"中,可使油茶组培苗在30天内发生出10.68条/株、平均根长为7.52㎝的根系,其发根率为100%。  相似文献   

鸡蛋花植物文化和国内栽培历史     

郭萌  宁熙平  张寿洲 《广东园林》2015,(1):65-68

鸡蛋花原产美洲热带地区,现已广泛种植于热带亚热带地区。除园林观赏外,其花可食用、药用,且寓意丰富。据考证,鸡蛋花在我国已有300多年的栽培历史。本文从自然分布、相关文化以及引入中国时间考证三个方面对鸡蛋花作系统而全面的介绍。  相似文献