倍他米松ELISA检测方法的建立     

郭璐  张晶  沙芳芳  赵兴然  王敬  王向红 《动物医学进展》2016,(12):50-54

为建立倍他米松(BET)ELISA检测方法,先使BET与琥珀酸酐反应,再采用活化酯法与牛血清白蛋白(BSA)偶联制备免疫原;其次,利用免疫动物获得多克隆抗体,经Sephrose 4B-proteinA方法对抗体进行纯化;最后,建立倍他米松ELISA检测方法。结果表明,免疫抗原偶联成功,获得了亲和力较高的多克隆抗血清,间接竞争ELISA测定其滴度为102 400、IC15为6.60×10-5 ng/mL和IC50为0.97ng/mL。该方法适用于残留在动物性食品中的BET现场大批量检测。  相似文献   

Fertility of Holstein cows and heifers submitted to timed artificial insemination and receiving one or two doses (12 h apart) of semen     

Miguel Mellado  Lucía M. Treviño  María I. Chavez  Francisco G. Véliz  Ulises Macías-Cruz  Leonel Avendaño-Reyes  José E. García 《Reproduction in domestic animals》2023,58(1):39-47

The objective of this retrospective study was to assess the effect of receiving a single (n = 50,285) or double (n = 4392) artificial insemination (AI), 12 h apart, within a timed artificial insemination protocol on pregnancy per AI (P/AI) in nulliparous heifers (inseminated with either sex-sorted or conventional semen) and pluriparous Holstein cows in a commercial dairy herd. Also, this study aimed to investigate the relationship between temperature-humidity index (THI) and time of the first AI and fertility. Fertility of cows receiving two AI with normothermia (THI <68) was higher (p < .05) than cows receiving a single AI (42.9% vs. 36.4%). P/AI of cows receiving two AI with severe heat stress (THI >85) was higher (p < .05) than cows receiving a single AI (21.0% vs. 12.6%). Regardless of heat stress conditions, applying the first AI in the morning increased (p < .05) P/AI in cows with double AI than in cows whose first AI occurred in the afternoon (38.4 vs. 33.3%). With moderate heat stress, and sexed-sorted semen, P/AI to timed AI was higher (65.0 vs. 51.9%; p < .05) in heifers receiving double AI than those serviced once. It was concluded that double AI, 12 h apart, enhanced fertility at timed AI than herd mates with a single AI, particularly with heat stress at breeding.  相似文献   

裸子植物体细胞胚胎发生和人工种子的研究   总被引：8，自引：0，他引：8  

杨映根  桂耀林 《种子》1995,(3):25-30

该文介绍了一种作为森林种子的大规模增值的有效方法－－裸子植物体细胞胚胎发生和人工种子的研制。其内容包括：（１）胚性愈伤组织的诱导与保存；（２）影响体胚发生的因素；（３）体胚的发育与成熟；（４）大规模培养体胚；（５）成熟体胚的干化、包理与人工种子生产；（６）体胚的萌发和转换。最后对人工种子的应用前景进行了讨论。  相似文献   

Flow Path Mathematical Modeling of Total Artificial Heart Test System     

ZHENG Xiao-lin  CAO Yong  DENG Xiao-yan  PENG Cheng-lin  LIAO Bin 《保鲜与加工》2007,(10):119-125129

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Expert System for Artificial Intelligence Blast Furnace Smelting Process     

SHI Jin-liang  JIA Bi  YU Qun-wei  LAN Shou-bing 《保鲜与加工》2005,(5):63

In order to upgrade traditional industry and realize the automation of furnace condition judgement and standardization of operation, an expert system for various furnace conditions and breakdown judgement and operation guidance on blast furnace are developed based on the theory of artificial intelligence and knowledge engineering. The system is fulfilled through C++Builder and combined with the actual production picture. The system has efficient and convenient man-machine interface by using menu operation method. The testing and operation of actual production data in simulation system indicate that the system is exact and reliable and can provide both the warning of various furnace breakdown and corresponding operation guidance.  相似文献   

An Expert System Approach to the Computer Aided Design of Intelligent Controllers     

Tu Yaqing Li Zushu Huang Shanglian 《保鲜与加工》1993,(2):24-29

The idea for an expert system approach to the computer aided design of intelligent controllers (ESCAD1C) is discussed and the idea of two step modelling of an intelligent controller is presented on the basis of emulating human control behaviours to solve complex problems. The structure, functions and characteristics of ESCADTC system , and the design procedures of intelligent controllers are gived. The knowledge base and simulating functions for designing intelligent controllers are also described.  相似文献   

人工合成甘蓝型油菜高频率再生研究初报     

易家玲  周清元  崔翠  李加纳  殷家明  林呐 《中国农学通报》2007,23(12):49-49

资源材料保存在作物遗传资源的研究与利用中起到非常重要的作用。该实验以不同来源的人工合成甘蓝型油菜KH106，KH109，KH111D为材料，以花序轴，子房，果柄为外植体研究其再生情况，结果表明，由芥蓝和小白菜人工合成的甘蓝型油菜KH106的再生频率极显著高于由羽衣甘蓝和白菜型油菜人工合成的甘蓝型油菜KH109和KH111；与花序轴和子房为外植体再生频率相比，以果柄为外植体平均再生频率最高，差异达到极显著水平；利用不同预处理培养基处理不同时间，结果显示不经过预处理直接接种到分化培养基上再生频率最高。  相似文献   

The Method of Diagnosing Brain-dysfunction Based on Membership Function and BP Neural Network     

Zeng Xiaoping  Zou Jiaqi  Zang Anbang 《保鲜与加工》1996,(3):44-49

Membership functions.formed with the characteristic values obtained by applying the continuous reaction time (CRT) theory from controls and heptic encephalopthy and proposedin this paper.whith these membership functions,the CRT data to be diagnosed are calculated in advance,and then discriminated by BP meural network to differentiate patients with or without braindysfunction.The troubles of low accuracy and efficiency,encountered in training BP network withfuzzy samples.scattered data and extended samples,are solved.  相似文献   

三江平原井灌水稻区地下水人工补给方法初探     

刘东  马永胜  付强 《农机化研究》2007,(5):222-224

针对三江平原井灌区地下水位逐渐下降、急需恢复的现状,结合国内外地下水人工补给实践,对三江平原井灌区地下水人工补给方法进行了探讨,提出了今后地下水人工补给实践的初步设想,为该区域地下水资源的可持续利用提供了参考依据.  相似文献   

自压式树状管网的两级优化设计模型与神经优化设计   总被引：4，自引：0，他引：4  

周荣敏  林性粹 《节水灌溉》2001,1(2):1-3

建立了自压式树状管网两级优化设计模型，并用人工神经网络法实现树状管网非线性规划模型的快速求解。采用的人工神经网络技术的两级优化设计模型在适用范围、求解速度和获得最优解能力上，均优于单一的非线性规划模型和线性规划模型，是实现树状管网全局优化设计的一条新途径。  相似文献