细胞表面展示有机磷水解酶的恶臭假单胞菌工程菌的构建及全细胞酶活性分析   总被引：3，自引：1，他引：3  

张红星  李茜茜  叶婷  李维琳  李林 《华中农业大学学报》2008,27(1):65-70

利用丁香假单胞菌(Pseudomonas syringae)冰核基因的N-末端(inpn)作为锚定单元(anchoring mo-tif),通过与来自黄杆菌属(Flavobacterium sp.)的有机磷水解酶基因(opd 构建融合基因inpn-opd,并连接于假单胞菌表达载体Pymbp,然后导入恶臭假单胞菌(P.putida)野生型菌株AB92019,获得了能在其细胞表面展示有机磷水解酶并具有全细胞酶催化活性的重组工程菌MMBL-opd.SDS-PAGE结果表明,融合基因能表达产生80 ku的蛋白质.重组菌MMBL-opd在无抗性LB固体培养基上能稳定生长,所携带的外源质粒的稳定性达到100%;在添加100 μmol/L Go2 培养基上28℃培养48 h,表面展示的有机磷水解酶具有最高全细胞酶活性,为0.036 U/mg.用蛋白酶K消化处理重组菌表面蛋白可使其全细胞酶活降低92%.重组菌在PBS缓冲液中于4℃条件下保存30 d,仍能保持93%的全细胞酶活.  相似文献   

Effect of temperature,organic amendment rate and moisture content on the degradation of 1,3‐dichloropropene in soil     

Robert S Dungan  Jianying Gan  Scott R Yates 《Pest management science》2001,57(12):1107-1113

1,3‐Dichloropropene (1,3‐D), which consists of two isomers, (Z)‐ and (E)‐1,3‐D, is considered to be a viable alternative to methyl bromide, but atmospheric emission of 1,3‐D is often associated with deterioration of air quality. To minimize environmental impacts of 1,3‐D, emission control strategies are in need of investigation. One approach to reduce 1,3‐D emissions is to accelerate its degradation by incorporating organic amendments into the soil surface. In this study, we investigated the ability of four organic amendments to enhance the rate of degradation of (Z)‐ and (E)‐1,3‐D in a sandy loam soil. Degradation of (Z)‐ and (E)‐1,3‐D was well described by first‐order kinetics, and rates of degradation for the two isomers were similar. Composted steer manure (SM) was the most reactive of the organic amendments tested. The half‐life of both the (Z)‐ and (E)‐isomers in unamended soil at 20 °C was 6.3 days; those in 5% SM‐amended soil were 1.8 and 1.9 days, respectively. At 40 °C, the half‐life of both isomers in 5% SM‐amended soil was 0.5 day. Activation energy values for amended soil at 2, 5 and 10% SM were 56.5, 53.4 and 64.5 kJ mol^?1, respectively. At 20 °C, the contribution of degradation from biological mechanisms was largest in soil amended with SM, but chemical mechanisms still accounted for more than 58% of the (Z)‐ and (E)‐1,3‐D degradation. The effect of temperature and amendment rate upon degradation should be considered when describing the fate and transport of 1,3‐D isomers in soil. Use of organic soil amendments appears to be a promising method to enhance fumigant degradation and reduce volatile emissions. Published in 2001 for SCI by John Wiley & Sons, Ltd  相似文献   

Close association between the enzymes involved in the lignin metabolic pathway of Sphingomonas paucimobilis SYK-6: interaction of O -demethylase (LigX) and ring fission dioxygenase (LigZ)     

Tomonori Sonoki  Yuichiro Otsuka  Seiichiro Ikeda  Eiji Masai  Shinya Kajita  Yoshihiro Katayama 《Journal of Wood Science》2002,48(3):250-252

  相似文献   

Resistance of bamboo scrimber against white-rot and brown-rot fungi     

Anuj Kumar  Pavla Ryparovà  Bohumil Kasal  Stergios Adamopoulos  Petr Hajek 《Wood material science & engineering》2020,15(1):57-63

ABSTRACT

Bamboo scrimber is one of the most emerging structural materials for future building applications and it possesses properties comparable to other natural wood-based engineered materials such as glulam, laminated veneer lumber and cross-laminated timber. The goal of this work was to study the decay resistance of bamboo scrimber against white-rot (Trametes versicolor) and brown-rot fungi (Serpula lacrymans). Bamboo scrimber samples were incubated in petri dishes with the wood-decaying fungi and the weight loss after 12 weeks was measured. The surface morphology of fungal-degraded bamboo scrimber was evaluated using optical microscopy. Based on the percentage weight loss, bamboo scrimber could be classified as highly resistant against bio-deterioration by white and brown-rot fungi.  相似文献   

一株丙硫菌唑降解菌的筛选及其降解条件的优化   总被引：1，自引：0，他引：1  

林凤翔  叶壮  胡芃  王莹  赵素霞  魏东  施艳红 《安徽农业大学学报》2022,49(2):279

从活性污泥中筛选出以丙硫菌唑为唯一碳源的降解菌株,对该菌株进行形态学、生理和生化特征结合16S rDNA基因序列鉴定为假单胞菌属绿脓杆菌,命名为 Pseudomonas aeruginosa W-313。通过响应面法对菌株W-313降解丙硫菌唑的条件进行优化,初始pH值为7.40,温度为 32 ℃,葡糖糖含量为0.60%时,W-313降解丙硫菌唑效果最佳,48 h降解率可达65.12%,与实际情况下基本吻合。研究结果为利用环境微生物降解丙硫菌唑及其代谢物进而有效规避残留农药污染提供新理论依据。  相似文献   

Biodegradation of Monoethanolamine,Ethylene Glycol and Triethylene Glycol in Laboratory Bioreactors     

Mrklas  Ole  Chu  Angus  Lunn  Stuart  Bentley  Laurence R. 《Water, air, and soil pollution》2004,159(1):249-263

The release of alkanolamines and glycols into the subsurface soils poses a potential hazard to the environment through impacted soil and groundwater. This study investigated aerobic and anaerobic biodegradability of monoethanolamine (MEA), ethylene glycol (MEG) and triethylene glycol (TEG). Significant levels of MEA (31 000 mg/kg), MEG (500 mg/kg) and TEG (2100 mg/kg) were successfully aerobically biodegraded in bioreactors. The aerobic slurry experiments suggested initial phosphate (P) limitation, as biodegradation rates increased by one order of magnitude after phosphate addition. Anaerobic decay of MEA, MEG and TEG was unaffected by P-addition. MEA, MEG and TEG degradation products such as acetate, ethanol and ammonium at about 75 000 mg/kg, 8100 mg/kg and 8800 mg/kg degraded completely and did not prevent aerobic biodegradation. This study confirms proposed biodegradation pathways of MEA, MEG, TEG and their breakdown products in natural soil and groundwater using indigenous microbes. Levels of contamination studied here are significantly higher than previously reported.  相似文献   

细胞电融合构建高效蒽降解重组菌株的研究     

魏明宝  魏丽芳  李军  郑先君 《农业环境科学学报》2006,(Z2)

通过An815与带EGFP标记的铜绿假单胞菌(Pseudomonas aeruginosa/EGFP)原生质体电融合,得到一株融合子R2,该融合子不仅具有An815菌高效降解蒽的能力,而且具有铜绿假单胞菌产生生物表面活性剂的能力,在72 h内将蒽从100 mg.L-1降解至27.9 mg.L-1,降解能力比出发菌An815提高了22%。  相似文献   

藤黄微球菌AD3的阿特拉津降解基因检测与分析     

徐胜文  郭玉华  王松文  李颖  蔡宝立 《沈阳农业大学学报》2007,38(4):564-567

为了获得高效稳定的阿特拉津基因,分离出更多的阿特拉津降解菌,试验采用PCR基因扩增和氮源利用方法,对AD3菌株的阿特拉津降解基因进行了检测和测序,并与其他菌株阿特拉津降解基因的序列进行了比较。结果表明：Micrococcus luteus AD3菌株含有阿特拉津降解基因trzN,atzB,atzC和atzDEF。其中trzN基因中心区的序列与Arthrobacter sp.TC1的trzN完全相同,atzB和atzC基因中心区的序列与Pseudomonas sp.ADP的atzB和atzC完全相同。AD3菌株能以氰脲酸为唯一氮源生长,Micrococcus luteus AD3菌株能将阿特拉津彻底降解成CO2和NH3。  相似文献   

铜绿微囊藻对苯酚的富集与降解   总被引：7，自引：0，他引：7  

司友斌  吴在鹏 《安徽农业大学学报》2000,27(3):269-271

以苯酚为培养介质中的唯一碳源,研究铜绿微囊藻的生长和对苯酚的去除效果。结果表明,铜绿微囊藻能利用苯酚的碳进行光合作用,加快其生长;同时,铜绿微囊藻对苯酚有明显的富集与降解作用,富集量２４ｈ最大达２７．８５ｍｇ／ｇ,此后随时间的延长逐渐降低。铜绿微囊藻对苯酚的降解符合一级动力学方程,５ｄ降解率为６７．６％。  相似文献   

一株有机磷农药降解菌的分离、鉴定及降解酶基因的克隆     

王圣惠闫艳春  徐刚明李莹莹 《农业环境科学学报》2007,26(B03):84-88

从农药厂污水处理池中分离到一株能很好降解甲基对硫磷和对硝基苯酚的菌株Yw18,它能以甲基对硫磷或对硝基苯酚为惟一碳源生长,经鉴定,为苍白杆菌(Ochrobacterum sp.).用气相色谱法和分光光度法对Yw18的降解性能进行了研究,结果表明,在0.5 h内它对50 mg·L-1甲基对硫磷的降解率达90%以上,在8 h内能将50 mg·L-1对硝基苯酚完全降解.对该菌进行了系统发育研究,并用PCR法克隆其甲基对硫磷降解酶基因mpd.  相似文献