Three-dimensional electroanatomic mapping and radiofrequency catheter ablation of ventricular arrhythmia in a dog without structural heart disease     

《Journal of Veterinary Cardiology》2022

A 1.5-year-old, female-spayed mix-breed dog was presented with recurrent episodes of shaking and excessive panting attributed to drug-refractory ventricular arrhythmia (VA) characterized predominantly by incessant periods of ventricular bigeminy. The VA had a narrow QRS morphology, suggestive of an origin near the His bundle or fascicular system. Diagnostic evaluation found no structural heart disease or underlying etiology. Three-dimensional electroanatomic mapping and radiofrequency catheter ablation were pursued. Voltage mapping demonstrated normal bi-ventricular voltage (≥1.5 mV) without any fractionated or multicomponent electrograms, indicating the absence of ventricular myocardial scar. Pace mapping identified an endocardial origin of the VA at the basal anterior septum of the left ventricle, distal to the His bundle and near the left bundle branch. Two ablation lesions were delivered to this site, and a left bundle branch block was temporarily induced. The dog recovered uneventfully. One month later, the owners reported a remarkable improvement in clinical signs, and follow-up 48-h Holter monitor found complete resolution of VA.  相似文献   

玉米雄穗相关性状QTL分析          下载免费PDF全文

高晶  李海灵  田然  李新海  王建军  翁建峰  王振华 《玉米科学》2022,30(2):35-41,50

以掖478和齐319构建染色体片段代换系群体（CSSLs），分析石家庄、新乡、昌平、顺义4个地点的雄穗主轴长（TL）和雄穗分枝数（TBN）的表型鉴定数据。结果表明，在2个以上环境中共检测到27个雄穗主轴长QTL，分布在1、2、3、4、5、7、8、9和10号染色体上，其中，1号染色体上雄穗主轴长QTL位点最多；共检测到23个雄穗分枝数QTL，分别位于1、2、4、6、7、8和10号染色体，其中，1号染色体上雄穗分枝数QTL位点最多。验证了北方春玉米环境稳定的3个雄穗主轴长QTL和10个雄穗分枝数QTL，发掘到黄淮海环境特异的5个雄穗主轴长QTL和3个雄穗分枝数QTL。在4个环境下均能检测到5个雄穗主轴长QTL（qTL1-3、qTL1-4、qTL2-1、qTL3和qTL4-2）和1个雄穗分枝数QTL（qTBN1-7），可为控制雄穗主要性状的基因克隆和机理解析提供参考。  相似文献   

杨梅枝条醇提物对A375细胞增殖和凋亡的影响及其分子机制          下载免费PDF全文

郑园园  俞浙萍  张淑文  李有贵  孙鹂  郑锡良  戚行江 《浙江农业学报》2022,34(5):974

杨梅(Myrica rubra)具有较高的药用价值,利用杨梅枝条醇提物(Myrica rubra branch extract, MRBE)开展其对人恶性黑色素瘤A375细胞的作用研究。采用MTT法、PI流式细胞术、Annexin V-PI双染流式细胞术、实时荧光定量PCR(qRT-PCR)等方法,解析MRBE诱导A375细胞阻滞与凋亡的潜在作用机制。结果表明:MRBE对A375细胞的抑制率呈剂量依赖效应关系,抑制率最高达49.46%;流式细胞术结果表明,MRBE作用48 h可使A375细胞阻滞于S期并发生凋亡;qRT-PCR结果进一步表明,MRBE可能通过上调P21和下调CyclinA、CyclinD、CDK2、CDK4、CDK6、E2F1等基因的表达,诱导A375细胞阻滞于S期,同时下调Bcl-w和Raf基因的表达促使A375细胞凋亡。初步解析了MRBE对人恶性黑色素瘤A375细胞增殖和凋亡的作用机制,为利用MRBE研发抗黑色素瘤潜在药物提供科学依据,同时有助于提高杨梅枝条的药用附加值,拓宽杨梅产业链多元化发展。  相似文献   

添加桑枝木屑栽培榆黄菇的 培养料配方筛选试验     

张翠怡  刘宇  宋庆港  刘新康  徐晓玲  宋爽 《蔬菜》2022,(7):37-39

摘要：为降低榆黄菇的栽培成本，开发利用新型栽培基质，以桑枝木屑代替部分棉籽壳，设置3种不 同桑枝木屑添加量（10%、20%、30%）的栽培配方，探究添加桑枝木屑对榆黄菇生物学转化率和子实体 营养成分的影响。结果表明：供试配方中，添加10%桑枝木屑（棉籽壳32%、桑枝10%、玉米芯42%、麸 皮10%、豆粕5%、生石灰1%）栽培的榆黄菇生物学转化率最高，为88.76%，子实体中脂肪、可溶性膳食 纤维、碳水化合物和粗多糖含量较常规栽培配方分别提高了16.7%、112.5%、150%和44.71%。  相似文献   

珍珠李枝枯病病原鉴定及致病性测定     

黄荣  唐利华  郭堂勋  李其利  孙文秀  黄穗萍  陈小林  莫贱友 《植物病理学报》2022,52(5):863-866

In order to confirm the pathogen of branch blight on pearl plum and its virulence, 14 samples were collected from Guangxi and Guizhou Province, and 21 isolates were obtained and were identified as Botryosphaeriaceae. The results of colony morphology, conidial observation and molecular identification of internal transcribed spacer region (ITS1-5.8S-ITS2) and the translation elongation factor 1-α (EFl-α) showed that the pathogens of branch blight on pearl plum were Botryosphaeria dothidea (66.67%), Neofusicoccum parvum (19.05%), N. algeriense (9.52%), and Lasiodiplodia pseudotheobromae (4.76%). The pathogenicity of different species of Botryosphaeriaceae was tested by mycelial dishes inoculation, and it was found that typical isolates of the four species were pathogenic, among which L. pseudotheobromae was the most virulent one and B. dothidea was the weakest one. This is the first report that L. pseudotheobromae causes branch blight on pearl plum.  相似文献   

番茄枝叶修剪机械手视觉伺服控制系统研究     

梁喜凤  花瑞  杨铭涛 《中国农机化学报》2022,43(7):8

为解决番茄枝叶修剪机械手无法准确找到番茄侧枝修剪点的问题，保证番茄枝叶修剪机械手工作效率，提出基于不同边缘类型的番茄侧枝特征点识别方法。在粒子群算法优化的神经网络(PSO-BP)的基础上设计“眼在手上”视觉伺服控制系统。通过对番茄植株横纵边缘分割，分别进行横向边缘和纵向边缘的提取，用阈值分割除去番茄叶片的短边缘，并记录其交点横纵坐标，限制交点分别在横向边缘的上边缘和下边缘，得到对偶的番茄侧枝点。试验结果表明，所设计的视觉伺服系统能成功的识别番茄侧枝点，仿真试验测试得到图像特征的实际值和期望值误差在3~5个像素之间，五自由度的机械手能在4 s内移动到期望位置，为农业修剪枝叶机器人进一步研究提供参考。  相似文献