苹果种质资源对苹果树腐烂病抗性评价   总被引：4，自引：1，他引：3  

刘欣颖  吕松  王忆  王昆  李天红  韩振海  张新忠 《果树学报》2011,(5):843-848

为全面了解苹果种质资源对苹果树腐烂病的抗性情况,利用2个不同来源的苹果腐烂病菌株,连续2 a对204份苹果种质资源进行了枝条离体接种鉴定。结果表明,接种后不同的种质资源的发病率和严重度均表现出丰富的多样性。西洋苹果资源（Malus domestica Borkh.）中发病率较低-极低的品种和类型所占的比例较高（16.6...  相似文献   

广西莪术优良种质挥发油成分的聚类分析     

王建杨妮  朱锦萍 《广东农业科学》2015,42(2):89-93

利用聚类分析软件对150份不同种质桂莪术挥发油成分进行分析,为临床用药需要及选育优良桂莪术种质提供参考依据。采用GC-MS联用仪测定初步筛选出来的优良种质桂莪术挥发油成分,通过聚类分析软件筛选其特征挥发性组分并进行比较,得出不同种质桂莪术挥发油成分分析结果。结果表明:以不同变量为标准进行聚类分析,各不同种质桂莪术均能聚集成类。以莪术烯和β-榄香烯为变量均聚为3类,以莪术二酮为变量聚为2类,以莪术烯、β-榄香烯为变量聚为4类,以莪术烯、莪术二酮、β-榄香烯三者为变量聚类分为3类,以所有成分为变量聚为4类。  相似文献   

苎麻蝙蛾发生规律的研究     

薛召东  张继成等 《中国麻业》2002,24(5):20-22

苎麻蝙蛾（Phassus jianglingensis Zeng et Zhao）是近几年来严重为害苎麻蔸部的一种新害虫。它以幼虫蛀食苎麻地下茎,形成隧道,严重影响地上部分的生长;研究表明该虫在湖南年发生一代,以幼虫在地下茎的隧道中越冬;翌年4月中旬化蛹,蛹期25天左右;5月上旬羽化,成虫寿命6-10天,无趋光性,白天停歇,晚间也很少活动,无需补充营养。6月上中旬出现新一代幼虫,幼虫期可长达320天,有相互残杀的习性。  相似文献   

虎杖PcPKS1基因RNAi载体的构建     

柳忠玉  赵树进 《湖北农业科学》2015,(9)

虎杖(Polygonum cuspidatum Sieb.et Zucc.)是富含芳香族聚酮化合物的药用植物,Ⅲ型聚酮合酶(Polyketide synthase,PKS)对芳香族聚酮化合物的合成起重要作用。为了研究虎杖Ⅲ型聚酮合酶基因Pc PKS1的功能,分别选取Pc PKS1基因的编码区保守序列(574 bp)和3′端非编码区特异序列(209 bp)作为干扰片段。将选定的干扰片段,分别以正向和反向插入到p YLRNAi载体中GUS基因内含子的两侧,以形成hp RNA表达盒,成功构建了以组成型Ca MV35S为启动子,以潮霉素抗性为筛选标记的RNA干扰表达载体p YLRNAi-CDS和p YLRNAi-UTR,并将载体导入农杆菌LBA4404中。  相似文献   

温度对野蚕黑卵蜂卵巢发育和卵子发生的影响     

刘永华叶恭银  李鲜花 《昆虫天敌》2006,28(3):126-131

分别在19、22、25、28和31℃条件下,观察不同温度对野蚕黑卵蜂Telenomus theophilae Wu et Chen(Hymenoptera：Scelionidae)雌蜂卵巢发育和卵子发生的影响。结果表明,在19～28℃之间,雌蜂卵巢生长,基端卵子生长和卵巢中卵子发生的速度随温度上升而逐步加快。在31℃下,羽化1d后雌蜂卵巢长宽显著短于25℃和28℃下同日龄的的雌蜂,卵巢中成熟卵也显著减少,但从羽化后第2d开始,各项指标均与25℃和28℃下的接近。这说明该蜂对温度的适应范围较广,在19～31℃之间,虽然野蚕黑卵蜂的卵巢发育速度不同,但所能达到的最大卵量没有明显差异,说明这5个温度对于该蜂的卵子形成均较为适宜。  相似文献   

甘肃羌活资源特征及开发利用   总被引：10，自引：2，他引：8  

陈小莉  方子森  张恩和 《草业科学》2005,22(1):1-3

在多点、多次实地考察和查阅大量文献资料的基础上,对羌活Notopterygium incisum生物学特性、品种分类以及在甘肃的资源分布状况作了较系统的介绍,并对其开发利用进行了探讨.  相似文献   

Host immune responses after administration of inactivated Venezuelan equine encephalomyelitis virus vaccines—III. Kinetics for neutralizing antibody immunoglobulin class responses in donors and adoptively-immunized recipients     

Stanley G Rabinowitz  Jayashree Huprikar  Caroline Whitacre 《Comparative immunology, microbiology and infectious diseases》1979,1(4):295-303

C57B16 mice were immunized with either live, attenuated TC-83 strain VEE virus vaccine or formalin-inactivated VEE vaccine combined with Bordetella pertussis. The kinetics of specific donor and adoptively-immunized recipient anti-VEE neutralizing antibody responses were studied. Donor mice immunized with either live or inactivated VEE virus vaccine combined with potent adjuvants develop specific anti-VEE IgM and IgG responses as early as 7 days post-immunization. Anti-VEE IgM antibody responses comprise the majority of anti-VEE neutralizing antibody at this early time period. By 14 to 21 days post-immunization, anti-VEE IgG responses predominated. When adoptively-immunized recipients were studied, the anti-VEE IgM to IgG predominance seen in donors early after administration was reversed, and for each time-period studied, recipients' serum anti-VEE antibody class responses consisted principally of IgG rather than IgM antibody. Since T-cells cooperation with B-cells is critical in the IgM-IgG antibody shift, these studies support the critical role T-cells exert in adoptive transfer in a murine model of experimental VEE infection. Furthermore, immunization with either live or inactivated VEE vaccine coupled to a potent adjuvant induce comparable donor and adoptively-immunized recipient anti-VEE antibody class responses.  相似文献   

鲤鳃的组织学观察     

冯怀亮  郭文场  张仁成  常廷荣 《中国兽医学报》1991,(2)

应用光镜和电镜技术对鲤鳃进行了形态结构观察.结果表明,鲤鳃内有呼吸上皮、支持细胞、粘液细胞、泌氯细胞和蛋白腺细胞.本文除对上述细胞详细描述外,还对其结构特点与功能的关系进行了探讨.  相似文献   

An occurrence of Newcastle disease in pigeons: Virological and serological studies on the isolatesFoyers de maladie de Newcastle chez le pigeon: Etudes virologiques et serologiques sur des souches isolees     

F Biancifiori  A Fioroni 《Comparative immunology, microbiology and infectious diseases》1983,6(3):247-252

The antigenic and pathogenetic relationship between pigeon Newcastle disease virus (NDV) isolates during outbreaks of 1982 in Italy and reference pathogen and non-pathogen NDV-strains were investigated.The pigeon-isolates were slow-eluters and showed a thermostability at 56°C of over 30 min. They proved to be lentogenic as measured by the mean-death-time in chicken-embryos, and between lentogenic and mesogenic as measured by the Hanson test. They failed to produce plaques in chicken-embryo-fibroblasts and showed high pathogenicity for experimentally infected pigeons, low-pathogenicity for quails and were not pathogenic for chickens. They were antigenically different from the LaSota strain as measured by the cross-HI-test and induced considerable seroconversion in inoculated animals. The existence of a lentogenic neurotropic pigeon-pathogenic strain was considered.  相似文献   

补肾益寿胶囊药渣栽培鲍鱼菇及营养成分分析     

陈今朝  谭永忠  王新惠  王慧超 《西南农业学报》2012,25(2):740-742

用补肾益寿胶囊药渣、木屑为主要原料栽培鲍鱼菇,筛选最适栽培配方并测定鲍鱼菇的营养成分。结果表明,栽培鲍鱼菇的最佳配方是补肾益寿胶囊药渣40%、木屑40%、麦麸18%、石膏1%和蔗糖1%。此时,鲍鱼菇菌丝体的日均长速为0.34 cm/d,生物学效率为55.8%。鲍鱼菇碳水化合物、粗蛋白、粗纤维、灰分和粗脂肪的含量分别为48.80%、19.76%、8.12%、9.21%和13.25%。  相似文献