Promoting effect of botulinum neurotoxin serotype A heavy chain on neuritogenesis in cultured Neuro-2a cells     

GAO Mei-ling  WANG Hong  ZHANG Cai-yun  LAN Jing  LI Xia-qing 《园艺学报》2015,31(12):2221-2227

AIM: To observe the neuritogenic actions of botulinum neurotoxin serotype A heavy chain (BoNT/A HC) on cultured Neuro-2a cells and to investigate the related signaling mechanisms for the effect of BoNT/A HC. METHODS: Neuro-2a cells were treated with different doses of BoNT/A HC (0.01, 0.1, 1 and 10 nmol/L), and then the cells were harvested at 24 h, 48 h and 72 h of BoNT/A HC exposure for detecting the neurite length and the percen-tage of the cells with neuronal processes by immunofluorescence staining. The most efficient dose of BoNT/A HC was chosen for exposure to Neuro-2a cells as the above. Whole cell protein was harvested at different time points for detecting the protein levels of phosphorylated ERK1/2 (p-ERK1/2) and phosphorylated Akt (p-Akt) by Western blot. RESULTS: Low doses of BoNT/A HC stimulated the neurite outgrowth, and increased the percentage of the cells with neurites compared with the negative controls (P<0.05), especially in the group with 1 nmol/L of BoNT/A HC treatment. Meanwhile, the phosphorylation of ERK1/2 and Akt was increased after treated with BoNT/A HC. There was an increasing tendency for the phosphorylation of ERK1/2 after the exposure of the cells to BoNT/A HC. The obvious increase in p-ERK1/2 was seen from 60 min to 5 h with 1 nmol/L of BoNT/A HC treatment (P< 0.05), and the increased protein level of p-Akt was mainly observed at 15 min and 60 min (P<0.05). CONCLUSION: BoNT/A HC stimulates the neuritogenesis. The neuritogenic mechanism of BoNT/A HC on Neuro-2a cells might be realized by activation of the phosphorylation of ERK1/2 and Akt.  相似文献   

Copper localization in Cannabis sativa L. grown in a copper-rich solution     

Laura Arru  Sara Rognoni  Micaela Baroncini  Piera Medeghini Bonatti  Pierdomenico Perata 《Euphytica》2004,140(1-2):33-38

Summary With the aim to examine its potential as a renewable resource to decontaminate polluted soils, electron microscopy combined with X-ray microanalysis was used to investigate the localization of copper in Cannabis sativa grown in hydroponic copper-rich culture. Cu was found to accumulate preferentially in the upper leaf epidermal cells; it was also detected in spiculae and in abaxial trichomes too. Primary bast fibres seem to be not involved in copper accumulation.  相似文献   

覆盖措施对咸水结冰灌溉后土壤水盐动态和棉花生产的影响   总被引：3，自引：0，他引：3  

封晓辉  张秀梅  郭凯  谢志霞  刘小京 《棉花学报》2015,27(2):135-142

对咸水结冰灌溉融冰后不同防止返盐措施处理的土壤水盐动态以及棉花的出苗、生长特征和产量进行了研究。结果表明,不同的覆盖措施对抑制土壤表层水分蒸发的效果有显著差异,3月和4月份表层土壤含水量影响5月份的含盐量,而5月份表层土壤的含盐量直接决定棉花出苗率。地膜覆盖和保留前茬旧膜的处理保墒抑盐效果最好,棉花出苗率和产量也最高;秸秆覆盖也有一定的抑盐效果;而翻耕和中耕对土壤保墒抑盐的作用较差;液态膜处理的抑盐效果最差,棉花的出苗率和产量最低。可见保留前茬旧膜是简便有效的保墒抑盐途径。  相似文献   

锑矿区大叶女贞重金属富集特性研究     

童方平  李贵  杨勿享  石文峰  刘振华  陈瑞  吴敏 《中国农学通报》2015,31(30):244-248

为了选择重金属污染土壤生态修复植物,利用采样测试分析的方法,对生长于冷水江严重Sb、As、Cd、Hg污染土壤的大叶女贞进行了研究。结果表明:大叶女贞能耐较严重重金属胁迫,其生长量、生物量受影响较小,胸径、树高和生物量分别减少2.58%、6.07%、7.58%;大叶女贞对各重金属的富集系数均大于2,其中Hg、Cd的富集系数最大,Cu、As的富集系数最小;对各重金属的转运系数均大于60,其中Sb的转运系数最大,Zn的转运系数最小;各重金属在大叶女贞根、茎、叶器官内的富集量有极显著差异,根、茎、叶等器官间富集的重金属有极显著差异,其叶部富集重金属的顺序为ZnSbAsPbCuCdHg,茎部富集重金属的顺序为ZnSbPbCdAsCuHg,根部富集重金属的顺序为ZnSbPbAsCuCdHg,叶、茎、根三器官富集各种重金属的顺序为叶茎根,至少有93.0%重金属富集在叶、茎、枝等地上部器官,其中至少有60.0%的重金属富集在叶部;大叶女贞为一优良重金属污染土壤生态修复树种。  相似文献   

土壤重金属迁移模拟研究的现状与展望   总被引：16，自引：1，他引：16  

隋红建  吴璇  崔岩山 《农业工程学报》2006,22(6):197-200

随着工业发展和废弃物的排放不断增加，土壤重金属污染已经成为一个世界性环境问题。土壤重金属的分布转化以及污染修复的研究受到广泛关注，数学模型及计算机模拟研究成为重要的研究手段。该文对土壤重金属迁移的模拟研究的进展进行了回顾与归纳，集中阐述了对流弥散和经验随机两大类模型的特点和应用，并指出了研究中存在的问题及今后研究的发展方向。将数学模拟与土壤重金属信息系统及地理信息系统GIS的有机结合，将有可能为环境保护和农业决策提供新的重要依据。  相似文献   

重金属在小麦作物体中残留特征的田间试验研究   总被引：41，自引：4，他引：41  

冯绍元  邵洪波  黄冠华 《农业工程学报》2002,18(4):113-115

研究表明经污水灌溉后重金属铅、砷和镉在小麦植物体中的残留量因部位不同而有差异,但清水和污水灌溉对其总体残留特征无明显影响。其总的分布趋势是小麦根部的重金属累积量最大，其次是小麦的茎叶部，再其次是小麦的穗部，即小麦植株体内重金属积累量由大到小依次为根＞茎叶＞穗。因此认为根可作为一种屏障使重金属铅、砷和镉难于向上部迁移，使地上部分免受其害。  相似文献   

漳州市农业土壤重金属现状、分析及防治   总被引：20，自引：3，他引：20  

郭义龙  林壹兵  胡少宜 《土壤》2003,35(2):131-135

调查漳州市农业土壤重金属Hg、As、Cr、Cd、Pb背景值及污染现状,调查结果,漳州市农业土壤重金属背景值相对较低。其现状为,Hg、As、Cd、Pb含量高于背景值1~4倍(Cr除外),特别是Hg和As为高污染元素。按土壤类型划分,水稻土土类重金属含量高于赤红壤土类(Hg除外);按土地利用方式划分,水田>菜地>果园;按流域划分,九龙江流域农业土壤重金属含量高于非九龙江流域(Cr除外)。污染源以农业污染为主,工业污染相对较轻。  相似文献   

某硫矿重金属分级及土柱淋溶   总被引：4，自引：0，他引：4  

C. LIN  J. LIN 《土壤圈》2003,13(1):75-80

Fractions of various heavy metals in a sulfidic minespoil were investigated.Column leachine experiment was also conducted to simulate “acid mine drainage“(AMD) from the minespoil.The results show that leaching of heavy metals from the minespoil was extremely significant during the initial water flushing.The amounts of heavy metals leached out dramatically reduced after leaching twice.It is worthwhile to note that in this study,Zn,Mn,Fe,As and Ni in the first leachate exceeded the total amount of each corresponding water-extractable(1:5,soil:water)metal contained in the minespoil sample.This appears to suggest that 1:5 water extraction did not allow accurate estimation of water-leachable concentrations of the above heavy metals.This work has implications for the management of sulfidic minespolis.Acid drainage of great environmental concerns is likely to occur only during heavy rainfall events after substantial soluble and readily exchangeable acid and metals are accumulated in the minespoils.The slow-reacting fractions other than water-soulble and readily exchangeable fractions may pose little environmental hazards.This is particularly true for Pb,As and Ni.  相似文献   

天津市不同污染梯度树木吸滞重金属及硫能力分析     

陈波  鲁少波  李少宁  鲁绍伟  石媛  李利学 《水土保持研究》2016,(1):175-182

以天津市主要绿化树种为研究对象,对叶片中重金属元素和硫含量进行了分析。结果表明:各研究区域内重金属污染程度为:昆仑桥(市区)水上公园(近郊公园区)蓟县(远郊园林区)杨柳青庄园(远郊风景区),随着市中心到远郊污染程度降低,不同区域各树种叶片吸滞重金属程度也随之降低,4个区域树木叶片吸滞污染元素排序大致均为:SAsPbCuCrCd;在昆仑桥柳树对各元素的吸滞能力均比较强,槐树吸滞As和Cr能力较强,杨树吸滞Pb和Cr能力较强,白蜡吸滞Cu和As能力较强;水上公园桧柏吸滞Cd和Cr能力较强,龙柏吸滞Cd和As能力较强,白蜡对Pb和Cu吸滞能力较强,柳树对S吸滞能力较强,雪松对各种元素的吸滞能力较强,杨树吸滞各元素能力相对较弱;杨柳青庄园桧柏吸滞Cr能力较强,雪松和龙柏吸滞Pb能力较强,杨树对Cd和Cu吸滞能力较强,杜仲对As和S吸滞能力较强;蓟县雪松吸滞Cr的能力较强,杨树吸滞Cd,S和Cu的能力较强,柳树对各元素的吸滞能力均较强,龙柏对各元素吸滞能力均相对较弱。  相似文献   

畜禽粪中重金属环境行为研究进展   总被引：22，自引：0，他引：22       下载免费PDF全文

郝秀珍  周东美 《土壤》2007,39(4):509-513

随着畜禽养殖业向集约化、规模化发展,饲料添加剂被大量使用,畜禽粪中重金属问题日益严重.本文综述了国内外畜禽粪中重金属污染状况、施用后对土-水-生物系统的影响、堆肥过程中重金属的迁移分配以及减少重金属污染的控制措施,为有机食品发展和养殖业废弃物安全利用提供参考.  相似文献