H3N2亚型犬流感病毒HA1基因的原核表达及抗原性分析     

张敦伟  熊永忠  远立国  贾坤  张桂红  李守军 《中国预防兽医学报》2011,33(2)

为原核表达H3N2亚型犬流感病毒(CIV)HA1蛋白,本研究利用特异性引物扩增CIV H3分离株的HA1基因,将其克隆到pMD18-T载体后进行序列测定。再将其亚克隆于pET-32a(+)中构建重组表达质粒pET-HA1。将该质粒转化于大肠杆菌BL21(DE3)中,经IPTG诱导,SDS-PAGE电泳分析,表达的重组蛋白约为58 ku。纯化的HA1蛋白经western blot和Dot-ELISA鉴定表明,表达的重组HA1蛋白可以与H3N2亚型CIV阳性血清发生特异性反应。  相似文献   

H5N1亚型禽流感病毒低剂量感染小鼠发病模型的建立     

李旭勇  郭晶  施建忠  李雁冰  陈化兰 《中国预防兽医学报》2011,33(7)

为模拟哺乳动物感染H5亚型高致病性禽流感病毒(HPAIV)的发病进程,本研究采用对哺乳动物高度致病的H5N1亚型HPAIV株A/bar-headed goose/Qinghai/3/05 (BHG/3/05),以低剂量鼻腔接种小鼠,观察发病、存活、病毒复制及组织病理损伤情况.结果显示,100.4 EID50即能够100％感染小鼠,但发病表现缓慢,死亡延迟至8d以后,存活达60％;体内病毒复制可持续10 d以上,感染后前3d病毒的增殖限于呼吸道,随后扩散至脑、脾、肾等其他器官;组织病理学观察肺脏早期表现出渗出性炎症,第10d发展为典型的间质性肺炎.本研究结果为探讨人禽流感的病理发生机制提供了具有价值的模型.  相似文献   

Implications of global and regional patterns of highly pathogenic avian influenza virus H5N1 clades for risk management     

Pfeiffer DU  Otte MJ  Roland-Holst D  Inui K  Nguyen T  Zilberman D 《Veterinary journal (London, England : 1997)》2011,(3):309-316

This paper analyses the publicly available data on the distribution and evolution of highly pathogenic avian influenza virus (HPAIV) H5N1 clades, whilst acknowledging the biases resulting from the non-random selection of isolates for gene sequencing. The data indicate molecular heterogeneity in the global distribution of HPAIV H5N1, in particular in different parts of East and Southeast Asia. Analysis of the temporal pattern of haemagglutinin clade data shows a progression from clade 0 (the ‘dominant’ clade between 1996 and 2002) to clade 1 (2003–2005) and then to clade 2.3.4 (2005 onwards). This process continuously produces variants, depending on the frequency of virus multiplication in the host population, which is influenced by geographical variation in poultry density, poultry production systems and also HPAI risk management measures such as vaccination. Increased multilateral collaboration needs to focus on developing enhanced disease surveillance and control targeted at evolutionary ‘hotspots’.  相似文献   

Vaccination of Poultry Workers: Delivery and Uptake of Seasonal Influenza Immunization     

R. Vivancos  D. Showell  B. Keeble  S. Goh  M. Kroese  A. Lipp  J. Battersby 《Zoonoses and public health》2011,58(2):126-130

Avian influenza is a highly infectious disease in poultry and although the risk of human infection is low, concerns exist that it could evolve into a new human strain of pandemic potential if reassortment with a human influenza virus occurs. In January 2007, the UK government introduced a programme to vaccinate poultry workers to reduce the potential of such an event. This study evaluates the delivery, uptake and costs of the programme in three counties of England. A questionnaire survey was completed by consultants in public health in all the Primary Care Trusts in Norfolk, Suffolk and Cambridgeshire in May 2007. The delivery of the programme varied between Primary Care Trusts, including being delivered in some cases by clinics in primary care, by general practitioners and occupational health services in others. The uptake of vaccination was low ranging from 7% to 29% at a cost of £29 to £132 per person vaccinated. Vaccination of poultry workers as a public health measure to prevent an influenza pandemic is likely to be ineffective with the level of coverage found in this evaluation in our region.  相似文献   

基因芯片技术检测5种马病毒   总被引：8，自引：0，他引：8  

朱来华  梁成珠  陆承平  吴华  杨元杰  马丰忠  王树峰  于红光 《农业生物技术学报》2006,14(2):203-207,i0002

通过分子克隆技术获得马疱疹病毒1型(Equineherpesvirus1,EHV1)、马动脉炎病毒(Equinearteritisvirus,EAV)、马流感病毒(Equineinfluenzavirus,EIV)、马传染性贫血病毒(Equineinfectiousanaemiavirus,EIAV)和东部马脑脊髓炎病毒(East-ernequineencephalomyelitisvirus,EEEV)等5种病毒各一段高度保守的特异性基因片段,用芯片点样仪逐点分配到处理过的玻片上,制备成检测芯片。提取样品中的RNA,进行反转录和荧光标记后滴加到芯片上进行特异性杂交,对杂交结果进行扫描检测和计算机软件分析。结果显示,制备的基因芯片可同时检测和鉴别上述5种病毒,可检测到阳性杂交信号的最高稀释度为10-6的病毒液,约25个病毒DNA拷贝,但其它病毒材料未见红色荧光信号,证明了本方法的特异性。在进口马的隔离检疫期间,采集马鼻肺炎、马动脉炎中和抗体阳性但病毒分离阴性马匹的白细胞悬液,分别在EHV1和EAV位点处可检测到阳性杂交信号。证明基因芯片技术不但快速、准确和敏感,而且可同时进行多种病毒的检测。  相似文献   

H1N1亚型猪流感诊断方法的研究新进展     

蔡春梅  翟少伦  温肖会  林美玲  严颖  周秀蓉  袁洁 《安徽农业科学》2015,(5):127-128,207

2009年,甲型H1N1流感在全球多个地方猪和人群中暴发,给人民群众的生命健康带来了巨大威胁,给经济发展和社会稳定产生了严重影响.及时快速的诊断是有效控制疫情大规模暴发的最有效途径之一.近几年随着现代分子生物学技术发展,猪流感诊断方法在快速、准确性方面有了很大提高.对H1N1猪流感概况及猪流感的诊断方法进行了综述.  相似文献   

环洞庭湖区活禽批发市场和水禽场H6亚型禽流感监测     

黄建龙  王昌建  邓国华  范仲鑫  何世成  朱春霞  唐小明  刘道新 《湖南农业大学学报(自然科学版)》2015,41(3)

为了解环洞庭湖区活禽批发市场和水禽场H6亚型禽流感病毒的分布和流行情况,分别于2009—2011年对环洞庭湖地区活禽批发市场、2011—2013年对水禽场的H6亚型禽流感进行了系统监测。对在活禽批发市场上采集的拭子样品先由尿囊腔接种SPF鸡胚,然后用血凝试验(HA)测定所收集的尿囊液,对HA测定有滴度的再进行血凝抑制试验(HI),并与RT–PCT方法相结合鉴定病毒的亚型。检测结果显示:在环洞庭湖区的5个活禽批发市场上都分离到H6亚型禽流感,并且在鸡、鸭、鹅拭子中都分离到该病毒,总的分离率为6.49%;水禽场H6亚型禽流感病毒群体阳性率达3.6%,拭子的H6亚型病毒分离率为0.29%,活禽批发市场鸭拭子H6亚型禽流感病毒分离率为9.58%,表明市场环节水禽H6亚型禽流感的隐性带毒率比养殖环节高,在一定程度上说明禽流感在活禽批发市场存在水平传播的风险。  相似文献   

Influenza in swine in Belgium (1969–1986) Epizootiologic aspects     

F. Haesebrouck  M. Pensaert 《Comparative immunology, microbiology and infectious diseases》1988,11(3-4):215-222

From 1984 until 1986, influenza isolates were obtained from 59 outbreaks of respiratory tract disease in Belgium. In 21 of the outbreaks, H₃N₂-influenza virus isolates, related to the human A/Port Chalmers/1/73 strain were obtained. All other isolates were H₁N₁-influenza virus strains. The prevalence of variants of the human H₃N₂-influenza virus in the Belgium swine population was determined by examining sow sera which had been collected between 1969 and 1984. The results of this serological study showed that, although a Port Chalmers-like strain was associated with outbreaks of respiratory tract disease in swine only since 1984, such strain was already present in the swine population in 1974, when a low percentage of sow sera (7%) reacted with A/Port Chalmers/1/73. Between 1975 and 1984, antibody against this strain were present in 28–61% of the sera. Furthermore, 3–6% of the sera collected between 1971 and 1980 reacted with the A/Hong Kong/1/68 strain. There were no indications that more recent human H₃N₂-strains (A/Texas/1/77, A/Bangkok/1/79 and A/Belgium/2/81) circulated in the Belgian swine population.  相似文献   

山东省2011年春季中标禽流感灭活疫苗质量检测及抗体消长规律分析     

艾武  亓丽红  王春玲  徐怀英  刘涛  李峰  杨英阁  王莉莉 《山东农业科学》2012,44(12):89-92

为了摸清山东省高致病性禽流感疫苗的质量情况,对2011年春季山东省3个中标厂家生产的重组禽流感病毒H5亚型二价灭活疫苗(H5N1,Re-5株+Re-4株)进行了质量检验和疫苗的抗体消长规律的跟踪。试验结果显示:除L厂家的2011010批次、G厂家的2011012批次有菌外,3个厂家的其余33批次灭活疫苗的物理性状、灭活检验和无菌检验均全部合格。免疫SPF鸡3周后,除Q厂家2011005批次疫苗外,其余34批疫苗HI抗体效价均≥6.0log2,产品达到质量标准要求。抗体消长规律实验表明,3个厂家的疫苗免疫SPF鸡4周即可达到高峰值,Re-4 HI抗体水平≥9.5log2,Re-5 HI抗体水平≥8.0log2,至25周时,仍维持在高抗体水平:Re-4 HI抗体水平≥9.0log2,Re-5 HI抗体水平≥7.8log2。从总的趋势看,L厂家的疫苗免疫抗体效价好于Q厂家和G厂家。  相似文献   

中药复方制剂抗猪流感病毒作用的研究     

张瑞华  徐彤  郑雪花  李寸欣  赵香汝  薛拥志  徐明举 《安徽农业科学》2012,40(11):6534-6536

[目的]探讨中药复方制剂(金银花、连翘、薄荷、牛蒡子、桔梗、甘草、淡竹叶、淡豆豉)对感染猪流感病毒小鼠的保护作用。[方法]猪流感病毒感染小鼠4 h后给药,以死亡率、平均存活时间、肺指数、肺指数抑制率、血凝滴度、脾脏指数、胸腺指数为评价指标,观察中药复方制剂在小鼠体内的抗流感病毒作用。[结果]中药复方制剂能降低流感病毒感染小鼠的死亡率(P0.01),延长小鼠的存活时间(P0.01),降低肺指数(P0.01),降低血凝滴度(P0.01),增加胸腺指数(P0.01)。[结论]该中药复方制剂具有良好的抗猪流感病毒作用。  相似文献