烟草NC89叶肉细胞原生质体再生植株的遗传稳定性   总被引：1，自引：0，他引：1  

陈曦 《安徽农业科学》1999,27(4):365-366

对烟草 Ｎ Ｃ８９ 叶肉细胞原生质体再生植株的 Ｒ０、 Ｒ１、 Ｒ２ 及 Ｒ３ 代的农艺性状进行了观察比较，测定了各代叶片中蛋白质和可溶性还原糖的含量，比较了各代植株的过氧化物同工酶谱，发现再生植株及其后代在农艺性状、蛋白质含量上均具有较好的遗传稳定性，过氧化物同工酶谱随代数的延长而趋于相同。  相似文献   

籼稻Kra Dang Ngah植株再生体系影响因素研究     

张银霞 《河南农业科学》2014,(11):27-31

以籼稻Kra Dang Ngah成熟胚为材料，探讨了培养基、植物凝胶质量浓度、激素配比、碳水化合物等因素对其再生体系的影响。结果表明，籼稻Kra Dang Ngah胚性愈伤组织的最佳分化培养基为ARDA培养基，植物凝胶为3.0 g／L，细胞分裂素（6 BA、KT）与生长素（NAA）配比为6∶1，碳水化合物为山梨醇15 g／L、水解酪蛋白1 g／L，在此条件下再生芽获得频率较高，为75.0％。将上述条件下获得的再生芽转移到生根培养基（MS培养基＋0.1 mg／L IAA＋30 g／L蔗糖）中可获得100％具有完全根系的再生植株，移栽炼苗7 d后生长1个月，再生植株的成活率达到100％。  相似文献   

黄波斯菊试管苗开花技术研究   总被引：1，自引：0，他引：1  

黄志明  刘才文 《江西农业学报》2011,23(3)

不同基本培养基对黄波斯菊试管苗成花试验结果表明:黄波斯菊的种子用0.1%升汞消毒8 min的效果最好,成活率为92%;最佳的增殖培养基配方为MS+6-BA0.2 mg/L+NAA0.02 mg/L+蔗糖20 g/L+琼脂10 g/L;芽苗在1/2 MS+NAA0.2 mg/L+PP3330.5 mg/L+糖20 g/L+琼脂10 g/L培养基中培养,植株表现为矮壮、叶片呈浓绿色、根系发达。取健壮的植株在改良MS+PP3330.5 mg/L+6-BA1.0 mg/L+糖20 g/L+琼脂10 g/L培养基上培养35～45 d后即可开花。  相似文献   

苹果砧木组培苗耐盐筛选技术研究   总被引：4，自引：1，他引：3  

王海英  孙建设  马宝盤  刘冬云  刘军侠 《果树学报》2000,17(3)

筛选了供试苹果砧木茎尖培养继代苗耐ＮａＣｌ的临界浓度，比较了０．６％ＮａＣｌ对继代苗、生根苗生长的影响。结果表明，继代苗的受害指数、相对增殖系数、相对生物产量、游离脯氨酸含量及细胞膜透性与砧木耐盐性之间存在一定的相关性，以盐胁迫 ５０ ｄ、盐敏感砧木受害指数高于０．７、继代苗生长严重受抑制为标准，确定继代苗耐ＮａＣｌ的临界浓度为０．６％－０．８％；继代苗、生根苗之间存在耐盐相关性。  相似文献   

马齿苋组培与耐盐筛选     

唐文杰  王玉  周泉澄  张边江 《黑龙江农业科学》2013,(3)

为了扩大耐盐植物资源,促进沿海滩涂植物资源利用,以马齿苋(Portulaca oleracea L .)的嫩叶和茎段为试验材料,研究了不同浓度激素和 NaCl 对愈伤组织生长与分化的影响.结果表明:MS +0.10 mg?L‐1 NAA 时,马齿苋茎段生根率较高,生长健壮;MS +2.00 mg?L‐16‐BA 是不定芽诱导的最适培养基;MS +2.00 mg?L‐16‐BA +1.00 mg?L‐1 NAA 是分化的最适培养基;在最适分化培养基中添加100 mmol?L‐1 NaCl ,诱导产生的愈伤组织为5%,大部分愈伤组织生长受抑制,同时也说明马齿苋具有一定的耐盐性.  相似文献   

Micropropagation of western hemlock (Tsuga heterophylla [Raf.] Sarg.) from embryonic explants     

Indra S. Harry  Chin-Yi Lu  Kiran K. Sharma  Trevor A. Thorpe 《New Forests》1994,8(1):1-13

An in vitro propagation protocol is described for western hemlock, an important forestry species in Canada. For shoot bud induction, embryonic explants were placed initially on one-third strength Schenk and Hildebrandt medium containing 5 M N⁶-benzyladenine (BA) or 5 M BA in combination with either 5 M kinetin or 5 M 2-isopentenyl adenine for 14 days. The explants were transferred to basal medium without cytokinins for 3 weeks, and then to basal medium containing 0.05% activated charcoal. Elongating shoots were subcultured every 4 weeks on charcoal medium. Shoots, 10 mm in stem height, were rooted either in agar or sterilized peat/perlite (1:1). Up to 70% of the shoots formed roots when they were transferred to the latter, moistened with 1/2 strength Gresshoff and Doy medium containing 5 M -naphthaleneacetic acid. About 90% of the plantlets survived transfer to greenhouse conditions.  相似文献   

Micropropagation of valley oak shoots from seedling explants     

K. R. Johnson  R. F. Walker 《New Forests》1990,4(4):271-279

Main and interactive effects of basal medium and cytokinin concentration on bud and shoot development of micropropagated valley oak explants were examined. Stem segments with axillary buds were placed on BTM (Broad Leaved Tree Medium), GD (Gresshoff-Doy) and WPM (Woody Plant Medium) media supplemented with 0.3, 0.7 and 1.5 mg 1^–1 of BAT (6-benzylaminopurine). Overall, BTM and GD media were superior for the micropropagation of this species, and both media promoted development of vigorous and relatively abundant shoots. Conversely, fewer shoots were initiated on WPM medium and those produced were frequently chlorotic. Irrespective of basal medium, the quantity of shoots produced after nine weeks generally increased as the concentration of BAP increased, but rosettes with short internodes and numerous leaves predominated on media supplemented with 0.7 or 1.5 mg 1^–1 of BAR Shoots on media with 0.3 mg 1^–1 of BAP, however, exhibited the long internodes and two or three leaves per shoot characteristic of typical valley oak sprouts, and were most suitable for subsequent micropropagation procedures.  相似文献   

植物组织培养中畸形苗发生机理的研究进展   总被引：4，自引：1，他引：4  

亓建飞  李付广  张朝军  刘传亮 《棉花学报》2004,16(4):243-248

畸形苗是植物组织培养中一种常见的生理异常现象,它严重制约着植物组织培养技术的发展。本文概述了植物组织培养中畸形苗的形态、发生机理及综合防治措施,并对进一步研究进行了展望。  相似文献   

怀地黄试管苗的快繁体系研究     

田伟伟  赵君 《安徽农业科学》2012,(5):2693-2695,2698

[目的]建立怀地黄试管苗的快繁体系。[方法]分别进行了NAA、6-BA和KT对茎尖培养以及NAA、6-BA试管苗快速繁殖的试验。[结果]中低浓度的NAA和6-BA可以在一定程度上促进怀地黄试管苗的快速繁殖,而高浓度的NAA和6-BA则会抑制怀地黄试管苗的快速繁殖;优选出的怀地黄试管苗的快速繁殖的最佳培养基为MS+0.002 mg/L NAA+0.2 mg/L 6-BA。[结论]试验优选出了怀地黄试管苗的快速繁殖的最佳培养基,为怀地黄的开发利用提供了理论依据。  相似文献   

海蓬子愈伤组织的诱导及植株再生     

余桂红  柏文娟  马鸿翔  张旭  易金鑫 《核农学报》2010,24(2):269-275

本试验研究了培养基成分对海蓬子愈伤组织诱导、继代培养、植株分化和生根的影响。结果表明:愈伤组织诱导的最适培养基是MS+Thidiazuron(TDZ)1.0mg/L+NAA1.0mg/L+蔗糖30.0g/L+琼脂7.0g/L;继代培养的最适培养基为MS+NAA1.0mg/L+TDZ0.1mg/L+蔗糖30.0g/L+Gelrite3.0g/L,愈伤组织快速生长;愈伤组织分化的培养基为MS+NAA1.0mg/L+TDZ0.1mg/L+BA2.0mg/L+蔗糖30.0g/L+琼脂7.0g/L+NaCl8g/L,愈伤组织分化频率可达62.2%。生根培养基中添加0.5mg/L的麦芽提取物(maltextract,ME)可显著增加每株的平均根数。NaCl促进海蓬子愈伤组织的分化,麦芽提取物可促进幼苗侧根的发生,但NaCl和麦芽提取物均不利于愈伤组织的诱导。本试验建立了较高频率的海蓬子愈伤组织诱导及植株再生体系。  相似文献