全文获取类型
收费全文 | 25728篇 |
免费 | 2063篇 |
国内免费 | 1938篇 |
专业分类
林业 | 3331篇 |
农学 | 1730篇 |
基础科学 | 465篇 |
2158篇 | |
综合类 | 9173篇 |
农作物 | 1126篇 |
水产渔业 | 4415篇 |
畜牧兽医 | 4847篇 |
园艺 | 1332篇 |
植物保护 | 1152篇 |
出版年
2024年 | 101篇 |
2023年 | 391篇 |
2022年 | 767篇 |
2021年 | 953篇 |
2020年 | 1026篇 |
2019年 | 1353篇 |
2018年 | 855篇 |
2017年 | 1440篇 |
2016年 | 1454篇 |
2015年 | 1190篇 |
2014年 | 1426篇 |
2013年 | 1788篇 |
2012年 | 2053篇 |
2011年 | 1902篇 |
2010年 | 1554篇 |
2009年 | 1415篇 |
2008年 | 1257篇 |
2007年 | 1380篇 |
2006年 | 1134篇 |
2005年 | 884篇 |
2004年 | 703篇 |
2003年 | 639篇 |
2002年 | 554篇 |
2001年 | 514篇 |
2000年 | 422篇 |
1999年 | 383篇 |
1998年 | 276篇 |
1997年 | 269篇 |
1996年 | 256篇 |
1995年 | 257篇 |
1994年 | 209篇 |
1993年 | 189篇 |
1992年 | 134篇 |
1991年 | 132篇 |
1990年 | 123篇 |
1989年 | 104篇 |
1988年 | 68篇 |
1987年 | 53篇 |
1986年 | 31篇 |
1985年 | 22篇 |
1984年 | 10篇 |
1983年 | 4篇 |
1982年 | 9篇 |
1981年 | 8篇 |
1980年 | 8篇 |
1979年 | 6篇 |
1978年 | 4篇 |
1977年 | 5篇 |
1976年 | 5篇 |
1956年 | 5篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
102.
103.
104.
105.
干旱胁迫下保水剂对苗木生长及生理的影响 总被引:10,自引:0,他引:10
在盆栽条件下,对不同保水剂处理的土壤吸持水特性以及云南拟单性木兰和绒叶含笑苗的水分生理、生长状况进行试验研究。结果表明,随着保水剂用量的增加,土壤保水能力和供水能力增加,苗木在有限水分条件下生存期延长3-91 d,地径、树高、新增叶片分别增加了16.7%-461.2%,22.7%-368.2%,42.9%-372.7%;随着保水剂用量增加,土壤容重下降了6.2%-35.1%,总孔隙度和毛管孔隙度明显增加了3.6%-20.3%和5.9%-22.6%;在有限水分条件下,通过不同保水剂处理能减少水分的胁迫,净光合速率增长8.9%-461.2%,蒸腾速率增长了22.2%-518.7%,气孔导度增长了4.2%-512.5%。 相似文献
106.
AIM: To explore the regulatory mechanism of nerve growth factor (NGF) on neurokinin A in the experimental asthmatic guinea pig. METHODS: Radioimmunoassay was used to determine the alteration of neurokinin A levels in the lower respiratory tract and visceral sensory afferent sites while NGF was absent (inhalation of NGF antibody through nasal cavity) in the asthmatic guinea pig. RESULTS: The contents of neurokinin A in the trachea, bronchus, lung, C7-T5 spinal ganglia and the correspondent spinal dorsal horn, nodose ganglia and solitary nucleus area in the experimental asthmatic guinea pig with the absent of NGF in the respiratory tract were much lower than those in the asthmatic and control groups (P<0.01). CONCLUSION: NGF upregulated the contents of neurokinin A in the lower respiratory tract and visceral sensory sites of the experimental asthmatic guinea pig, and both might be involved in the pathogenesis of asthma. 相似文献
107.
108.
AIM: To investigate inhibition of K562 cell growth by antisense drug targeted VEGF mRNA. METHODS: X7, 20-mer antisense sequences were selected, synthesized and modified with phosphorothioate. The drug was transfected into K562 cells in the present of lipofection. Cell growth was assayed by trypan blue dye exclusion assay and MTT. The level of VEGF protein in the media was determined by ELISA. The morphology of apoptotic cells were observed by Giemsa staining, and the propotion of apoptotic cells was detected by flow cytometry. RESULTS: The antisense drug inhibited growth of K562 and downregulated expression of VEGF protein significantly, compared with Scrambed control group and showed dose-dependent relation. Signs of apoptosis of K562 cells were not observed. CONCLUSION: Inhibition of K562 cell proliferation, but not cells apoptosis induction is the mechanism of inhibing growth of K562 cells by antisense drug targeted VEGF mRNA. At same time, VEGF has function of promoting K562 cell proliferation, and VEGF mRNA may be a new target attached by drugs. 相似文献
109.
AIM: This study was designed to investigate the secretion of VEGF and its receptor (flt-1 or flk-1/KDR) protein by cultured bovine thoracic aortic endothelial cells treated with various insulin concentrations. METHODS: Endothelial cells was isolated from bovine thoracic aorta, and cultured in serum-free medium, then incubated with different insulin concentrations (30 mU/L, 300 mU/L, 3 000 mU/L). The level of VEGF and its receptor (flt-1 or flk-1/KDR) protein were detected by immunohistochemical staining. RESULTS: As compared with no insulin group, the expression of VEGF protein in low insulin concentration (30 mU/L and 300 mU/L) groups were significantly increased (P<0.01). The expression of VEGF protein in high insulin concentration (3 000 mU/L) group was significantly decreased (P<0.05). Howerer, no difference of the expression of VEGF receptor (flt-1 or flk-1/KDR) protein among all groups (P>0.05) was observed. CONCLUSION: Low concentration insulin up-regulates the VEGF protein expression while high concentration insulin down-regulates the VEGF protein expression in bovine thoracic aortic endothelial cells, but insulin had no directly effect on the VEGF receptor (flt-1 or flk-1/KDR) protein expression in bovine thoracic aortic endothelial cells. 相似文献
110.
AIM: To study the expression of vascular endothelial growth factor (VEGF) in inflammatory mucosa of lacrimal sac. METHODS: Immunohistochemical S-P method was used to examine the expression of VEGF in the mocusa from 12 patients with chronic dacryocystitis and 8 volunteers. RESULTS: The positive rates of VEGF expression in different parts of the mocusa were: basal lamina: 44.3%±7.6%; surface epithelium: 16.9%±4.6%; connective tissue: 15.2%±4.9%, all normal mocusa of 8 cases were negative. There was a significant difference between the two groups (P<0.01), a significant difference among each part of the chronic inflammatory mocusa of lacrimal sac. CONCLUSION: VEGF may play an important role in hyperplasia of inflammatory mucosa of lacrimal sac. 相似文献