白肉灵芝水提物对脑缺血大鼠海马神经元的保护作用     

王昱  秦序  何九军 《中国畜牧兽医》2021,48(10):3864-3871

试验旨在探讨白肉灵芝水提物（Ganoderma leucocontextum aqueous extracts,GLAE）对脑缺血后海马神经元的保护作用及机制。将50只健康大鼠分为对照组、模型组、GLAE低（0.05 mg/（g·BW））、中（0.1 mg/（g·BW））、高（0.2 mg/（g·BW））剂量组。利用双侧颈总动脉夹闭法建立大鼠脑缺血模型,GLAE组灌胃不同剂量的GLAE干预,对照组和模型组灌胃同体积的生理盐水,连续2周。用跳台试验方法检测记忆获得、记忆巩固和记忆再现障碍大鼠的学习记忆能力,HE染色观察大鼠海马组织的病理形态的变化,比色法检测海马组织一氧化氮合酶（nitric oxide synthase,NOS）活性和一氧化氮（nitric oxide,NO）含量,Western blotting和实时荧光定量PCR法分别检测海马组织生长相关蛋白-43（growth associated protein-43,GAP-43）和脑源性神经生长因子（brain derived neurotrophic factor,BDNF）的水平。结果显示,与对照组相比,模型组大鼠跳台试验的逃避潜伏期显著缩短、电击次数显著增加（P<0.05）;海马神经元细胞出现明显核固缩、排列松散紊乱等退行性改变,细胞数量显著减少（P<0.05）;海马组织NOS活性和NO含量均显著降低（P<0.05）;大鼠海马组织GAP-43蛋白表达量显著升高（P<0.05）;海马组织BDNF mRNA表达量显著下调（P<0.05）。与模型组相比,GLAE干预后,大鼠逃避潜伏期均显著延长、电击次数均显著减少（P<0.05）;GLAE高剂量组大鼠CA1区和齿状回锥体神经元细胞形态明显改善,神经元数量显著增加（P<0.05）;GLAE低剂量组对NOS活性影响不明显（P>0.05）,显著增加NO含量（P<0.05）,GLAE中、高剂量组NOS活性和NO含量均显著升高（P<0.05）;GLAE低、中、高剂量组海马组织GAP-43蛋白表达量均显著增加（P<0.05）;GLAE低、中、高剂量组海马组织BDNF mRNA表达量均显著增加（P<0.05）。以上结果表明,GLAE可通过提高NOS活性和NO水平、促进海马神经发生和功能恢复对脑缺血后海马神经元损伤有一定的保护作用,从而改善大鼠认知功能,0.2 mg/g GLAE效果最好。  相似文献   

LM-BP神经网络水质评价模型应用研究——以新乡市卫河为例     

周振民  刘俊秀  范秀  郭威 《中国农村水利水电》2015,(7)

介绍了LM-BP神经网络模型的原理及算法和模型的优点。针对实际水质评价问题,利用随机内插方法在地表水环境质量分级标准阈值间生成训练样本和检验样本,建立了新乡市卫河地面水环境质量综合评价的LM-BP神经网络模型,将模型应用于卫河2011年3月份、9月份的水质评价,并与单因子评价法、模糊综合评价法进行了比较分析。实验结果表明该模型设计合理,泛化能力强,收敛速度快,算法稳定,推导严谨,有较充分的理论依据,应用于水质评价具有其合理性、实用性和有效性,适用于作深入的水环境质量分析。  相似文献   

Expression of fibroblast growth factor 23 by canine soft tissue sarcomas          下载免费PDF全文

M. R. Hardcastle  K. E. Dittmer 《Veterinary and comparative oncology》2016,14(3):306-317

  相似文献   

Polymorphism in promoter region of growth hormone receptor is associated with potential production capacity of insulin‐like growth factor‐1 in pre‐pubertal Holstein heifers          下载免费PDF全文

C. Kawashima  M. Munakata  M. Matsui  A. Miyamoto  K. Kida  T. Shimizu 《Journal of animal physiology and animal nutrition》2016,100(6):1037-1040

Insulin‐like growth factor‐1 (IGF‐1) is one of the important factors for growth, milk production and reproductive functions and mainly released from the liver in response to growth hormone (GH) via GH receptor (GHR) in cattle. Recently, some single nucleotide polymorphisms (SNPs) were identified in the bovine GHR gene. Some GHR‐SNPs were shown to be related to plasma IGF‐1 concentration in cattle. Hence, the capacity to IGF‐1 production in the liver might be affected by GHR‐SNP and associated with performance in the future. This study examined whether GHR‐SNP is associated with IGF‐1 production in the liver of pre‐pubertal heifers. In 71 Holstein calves, blood samples for genomic DNA extraction were obtained immediately after birth. To genotype the GHR‐SNPs in the promoter region, polymerase chain reaction (PCR) products were digested with restriction enzyme NsiI (cutting sites: AA, AG and GG). All heifers at 4 months of age were intramuscularly injected with 0.4 mg oestradiol benzoate. Blood samples were obtained from the jugular vein just before (0 h) and 24 h after injection. The number of AA, AG and GG at the NsiI site was 0, 17 and 54 respectively. In AG and GG, plasma GH concentrations were higher pre‐injection than 24 h post‐injection (p < 0.01). Moreover, plasma GH concentrations in AG post‐injection were higher than in GG (p < 0.05). In contrast, the GG genotype exhibited higher plasma IGF‐1 concentrations in pre‐injection than post‐injection (p < 0.01), although oestradiol did not change IGF‐1 concentration in the AG genotype. We conclude that the GG polymorphism in the promoter region of GHR is associated with a higher potential capacity of IGF‐1 production in the liver of cattle.  相似文献   

新疆喀什地区濒危物种裂盖马鞍菌生态因子调查     

李传华  王海孝  宋晓霞  刘长安  李泰辉  陈明杰  张劲松  马丹丹 《食用菌学报》2016,(4):60-65

在2013～2015年期间,每年3月31日到5月28日,调查新疆喀什地区裂盖马鞍菌(Helvella leucopus)的发生情况和环境因子,持续3年调查结果表明:在胡杨林和毛白杨中均有子实体发生,裂盖马鞍菌子实体发生在土壤pH 8.0左右、有机质含量20～36 g/kg、全氮含量0.8～1.3 g/kg和速效钾含量189～293mg/kg的地区;野生子实体形成期的土壤温度5.0～26.4℃,空气湿度20％～35％,光照3000～8000 lux.  相似文献   

Cyclooxygenase (COX) inhibitors and the intestine   总被引：1，自引：0，他引：1  

Little D  Jones SL  Blikslager AT 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(3):367-377

Nonsteroidal anti-inflammatory drugs (NSAIDs) have long been used for the treatment of pain and inflammation because of their inhibitory effects on cyclooxygenase (COX). For almost as long as NSAIDs have been in use, multiple adverse effects have been noted. Assessment of many of these adverse effects have been complicated because of the discovery of multiple splice variants of the cox gene, and a greater array of COX inhibitors, especially the COX-2 selective inhibitors have become available. Some of these adverse effects cannot be readily explained by the effect of these drugs on COX. This has sparked a new field of investigation into the COX-independent effects of the COX inhibitors. The major noncyclooxygenase targets of the COX inhibitors of particular relevance to inflammation and the gastrointestinal tract are phosphatidylinositol 3'-kinase Akt signaling, uncoupling of oxidative phosphorylation, PPARgamma, nuclear factor KB, mitogen activated protein kinases, and heat shock proteins.  相似文献   

葡萄VvSUC12基因启动子的克隆及上游调控因子鉴定     

吴佳鸿  邢启凯  周悦妍  白庆荣 《植物病理学报》2023,53(1):52-60

枝干病害葡萄溃疡病近年来严重限制了葡萄产业发展。研究表明葡萄蔗糖转运蛋白参与寄主植物和病原菌的互作过程。为解析蔗糖转运蛋白VvSUC12在葡萄免疫反应过程中的功能,本研究克隆了VvSUC12基因翻译起始位点上游1 500 bp的启动子区,通过生物信息学分析发现该区域包含4个Dof转录因子结合序列(A/TAAAG)及多种激素调节与防御相关的顺式元件。实时荧光定量分析显示,接种可可毛色二孢菌显著诱导VvSUC12和VvDof19基因的表达。通过酵母单杂交实验筛选得到与VvSUC12启动子互作的转录因子VvDof19。酵母双杂交实验证实VvDof19具有自激活活性;进一步通过烟草瞬时表达发现Dof19-GFP的相对GUS活性约为对照GFP的9倍,表明转录因子VvDof19能够激活VvSUC12基因的表达。研究结果为深入研究蔗糖转运蛋白在葡萄免疫反应中的功能奠定基础。  相似文献   

氟吡呋喃酮与8种杀虫剂复配对温室白粉虱的联合毒力     

金永玲#  刘媛媛#  王星宇  李加奇  陈云  张祖铭  相龙鑫 《植物保护》2023,49(2):383-388

为了筛选出对温室白粉虱高效的农药复配组合,本试验选择9种杀虫单剂对田间采集的温室白粉虱进行了毒力测定,并利用共毒因子和共毒系数对氟吡呋喃酮与8种杀虫剂复配组合的联合毒力进行评价,同时对筛选出的药剂进行田间药效试验。结果表明：杀虫单剂毒力测定中,LC₅₀最低的为鱼藤酮,最高的为螺虫乙酯,48 h LC₅₀分别为0.43μg/mL和78.59μg/mL;毒力大小顺序为鱼藤酮>氟啶虫胺腈>除虫菊素>苦参·印楝素>d-柠檬烯>氟吡呋喃酮>啶虫脒>高效氯氰菊酯>螺虫乙酯。共毒系数大于120的复配组合为氟吡呋喃酮与d-柠檬烯5∶1、氟吡呋喃酮与苦参·印楝素1∶2和1∶5,共毒系数分别为268.31、247.80和241.46,LC₅₀分别为5.76、4.51μg/mL和4.34μg/mL。氟吡呋喃酮与d-柠檬烯5∶1复配增效作用明显,为最佳配比。田间药效试验表明,10 d后,17%氟吡呋喃酮可溶液剂与1%苦参·印楝素乳油、5%d-柠檬烯可溶液剂复配对温室白粉虱的防效达到83.5%～83...  相似文献   

欧盟、美国和日本加工农产品中农药残留评估现状     

张峰祖  朴秀英 《植物保护》2023,49(2):13-21

本文介绍了欧盟、美国和日本评估加工农产品中农药残留的数据要求和试验准则,以及开展膳食摄入风险评估和推荐农药最大残留限量(MRL)的方法,汇总了用于农药残留评估的加工农产品及其分类,分析了加工农产品中农药MRL制定现状,为进一步提高我国相关领域技术提供借鉴。比较分析结果表明,欧盟和美国在农药登记资料要求中明确提出了加工试验数据要求并且制定了相应的试验准则,美国在试验准则中描述了加工农产品中农药MRL的推荐原则;美国和日本制定了一定数量的加工农产品中农药MRL,欧盟建立了适用于监管农药MRL的加工因子数据库;欧盟、美国和日本均在相关法规中规定了加工农产品中农药残留的合规性判定。  相似文献   

A HYBRID METHOD FOR CALCULATING THE STRESS INTENSITY FACTORS OF PLANE MIXED-MODE CRACK THROUGH BEM     

Wang Qizhi 《保鲜与加工》1992,(3):85-91

  相似文献