Resistance in Cucumis sativus L. to Tetranychus urticae Koch. 4. The genuineness of the resistance     

O. M. B. De Ponti 《Euphytica》1978,27(2):435-439

Summary For several generations subpopulations of the twospotted spider mite were reared on cucumber varieties previously selected as partially resistant. Subsequently resistance tests were carried out in the laboratory and the glasshouse with mites from these subpopulations and concurrently with mites from a basic population reared on a susceptible cucumber line. In these tests neither acceptance nor net reproduction or damage index on the partially resistant varieties depended on the kind of mite populations used as inoculum. Therefore it is concluded that the resistance is genuine. The stability of the resistance is discussed.  相似文献   

Resistance in Cucumis sativus L. to Tetranychus urticae Koch. 2. Designing a reliable laboratory test for resistance based on aspects of the host-parasite relationship     

O. M. B. de Ponti 《Euphytica》1977,26(3):641-654

Summary The relationship between the twospotted spider mite and cucumber has been studied on plants and on leaf disks of a number of varieties with different levels of resistance. Existing laboratory tests are critically discussed and it appears that they are only reliable if many factors are taken into account. A new, more efficient laboratory test for resistance, measuring acceptance and reproduction is described.  相似文献   

Resistance in Cucumis sativus L. to Tetranychus urticae Koch. 7. The inheritance of resistance and bitterness and the relation between these characters     

O. M. B. De Ponti  F. Garretsen 《Euphytica》1980,29(3):513-523

Summary The inheritance of resistance to the twospotted spider mite and of bitterness in cucumber has been studied in three sets of P₁, P₂, F₁, F₂, B₁₁ and B₁₂ of crosses between three bitter, resistant lines and one non-bitter, susceptible line. Resistance to the twospotted spider mite as measured by acceptance and oviposition appeared to be determined by several to many genes, which are inherited mainly in an additive fashion. Bitterness is basically governed by the gene Bi, which, contrary to earlier reports, is inherited in an intermediary way, while the expression of Bi is influenced by additively inherited intensifier genes. Whereas Bi and the bitterness intensifier genes are not related to the resistance factors acceptance and oviposition, they are related to resistance or tolerance as measured by the damage index. This relation is explained by linkage rather than by identity of the genes concerned. Changes in the test methods and breeding consequences are discussed.  相似文献   

朱砂叶螨抗药性研究   总被引：19，自引：0，他引：19  

吴孔明  秦夏卿 《华北农学报》1990,5(2):117-123

1986～1987年测定了河南省12个产棉县、市棉田朱砂叶螨对当前主要杀螨剂的抗性水平和多种农药对抗性叶螨的毒力水平.结果显示,朱砂叶螨已对三氯杀螨醇和氧化乐果产生抗性,各棉区基本处于抗性上升阶段.其中对三氯杀螨醇的抗性相对倍数较高,LC_(50)和LC_(95)值分别是相对敏感品系的1.72～8.26和3.47～17.67倍.从抗性品系对23种农药的感受性看出,朱砂叶螨已形成对硫磷高抗品系,抗性倍数达466.8倍;对磷胺、久效磷等也表现较高的抗性,但对甲氰菊酯、PP321、克螨特等尚未产生明显抗性,三环锡、Nissorun、毒死蜱、来福灵等有较好的杀若螨作用,对卵的抑制作用以Nissorun为最强,其次为浏阳霉素和杀虫脒.  相似文献   

在大肠杆菌内高效表达大分子量拟蜘蛛牵丝蛋白     

许红韬  樊宝良  曹更生  胡晓湘  李宁 《农业生物技术学报》2006,14(4):522-525

依据已报道的蜘蛛(Nephilaclavipes)牵丝蛋白部分cDNA序列,设计合成两种不同结构的拟蜘蛛牵丝蛋白基因单体,大小分别为360和390bp。多聚化得到8倍体和16倍体后,克隆到表达载体pET-30a,得到4种表达载体,转化大肠杆菌(Escherichiacoli)BL21(DE3)诱导表达。用自制的抗蜘蛛牵丝蛋白血清Westernblot检测,表达产物呈梯度排列,主带与预计大小一致。蜘蛛牵丝蛋白表达量最高为800mg/L。  相似文献   

蜘蛛毒素对人胃腺癌细胞株BGC-823增殖的抑制作用   总被引：1，自引：0，他引：1  

靳祎  鲍娜  袁洪水  高莉  宋大祥  朱宝成 《河北农业大学学报》2007,30(2):90-92

为比较不同蜘蛛毒素对肿瘤细胞的抑制作用,采用四甲基偶氮唑盐比色法(MTT法)测定了不同浓度的虎纹单柄蛛(Ornithocton huwen)、海南单柄蛛(Ornithoctonus hainana)、敬钊缨毛蛛(Chilobrachy jingzhao)和雷氏大疣蛛(Macrothele raveni)4种蜘蛛毒素对人胃腺癌细胞株BGC-823的抑制作用。结果表明:4种蜘蛛毒素对人胃腺癌细胞株BGC-823的细胞增殖均有不同的抑制作用;同一蜘蛛毒素浓度越高抑瘤作用越好,蜘蛛毒素作用时间越长抑瘤效果越强,量效关系和时效关系良好;不同蜘蛛毒素对BGC-823细胞增殖的抑制作用效果不同,以敬钊缨毛蛛毒素作用最强,IC50为0.109μg/μL。  相似文献   

山楂叶螨抽样技术   总被引：1，自引：0，他引：1  

胡全胜  徐瑞琳 《安徽农业大学学报》1988,(4)

本文在空间格局研究的基础上,对山楂叶螨种群密度估计、不同密度下理论抽样数及序贯抽样技术几方面进行了比较研究。研究结果表明:山楂叶螨种群密度可依m=1.7677(P_0~(0.5657)-1)进行简易的估计;其理论抽样数可据n=1/(D~2)2.0859m~(-0.3952)或n=1/(D~2)(2.1991/m+0.5377)确定;序贯抽样可依Iwao法确定的两条截止线T_0(n)=3n±6.6283n~(1/2)女和据Kuno法确定的最大抽样数结合起来使用。  相似文献   

荆州春季菜田及果园蜘蛛多样性初步研究   总被引：1，自引：0，他引：1  

姜清奎  李传仁  王福莲 《湖北农学院学报》2003,23(4):245-247

对荆州郊区春季露地菜田和果园蜘蛛的群落结构和多样性进行了初步研究，结果表明：菜田和果园蜘蛛分别有10科15属16种和8科11属12种；优势种均为T纹豹蛛；多样性果园高于菜田。  相似文献   

新疆跳蛛记述   总被引：1，自引：0，他引：1  

周丽娜  宋大祥 《新疆农业大学学报》1988,(3)

跳蛛科是蜘蛛目中一个大的类群,种类和数量繁多,是农林害虫天敌的重要组成部分,但迄今尚无系统的报道。本文记述新疆跳蛛16种,其中黑色艾卢蛛、镰猎蛛、安东莫鲁蛛、光滑新跳蛛、捆灰蛛、查氏似蚁蚁等7种为我国新纪录,前斑蛛、黑斑蝇狼、波跳蛛和斑点野跳蛛等4种为新疆新纪录。其它5种可供读者鉴别参考,故一并加以报道。文中还指出一些同物异名,有助于今后进一步修订种类。  相似文献   

棉叶螨在棉株上的垂直分布及其二阶抽样技术研究   总被引：2，自引：0，他引：2  

范晖  张淑莲  张美荣  陈志杰  王兴林 《西北农业学报》1997,6(3):61-64

根据田间调查结果分析,在棉花苗期,棉叶螨的垂直空间分布格局呈聚集分布,且具有明显的层次性,主要分布在倒1至倒5叶上,约占总螨量的90%左右,不同田块之间差异不显着。应用Iwao根据M^*-m关系提出的二阶抽样模型估测,在D达0.10、0.15、0.20精确水平时,调查顶1叶至倒5叶层螨量所需抽查的棉株数平均分别为109.6、48.9、27.5株。置信度取95%时,区间估计p上为0.9386～0.9674,p上为0.9530;p下为0.0374～0.0618,p下为0.0518.  相似文献