不同生长年限的草坪土壤酶活性研究   总被引：3，自引：0，他引：3  

蒲小鹏  李春荣  白小明  邹雨 《甘肃农业大学学报》2008,43(4)

通过对不同生长年限草坪土壤中4种土壤酶活性的分析,研究了草坪年限与土壤酶活性的关系.结果表明:过氧化氢酶活性随草坪生长年限的不同变化很小,而过氧化物酶、多酚氧化酶和脲酶的酶活性随草坪生长年限的不同差异显著;在建坪的第1 a,过氧化物酶和脲酶的酶活性较对照变化明显,二者分别下降46%和43%;多酚氧化酶较对照增加7%.生长2 a的草坪和生长4 a的草坪土壤中,过氧化物酶均比对照增加13%和28%,脲酶比对照增加33%和66%,过氧化氢酶活性均比对照降低约4%,多酚氧化酶在生长4 a的样地较对照下降约8%.因此,4种土壤酶中过氧化物酶和脲酶对土壤肥力变化的反映较敏感.  相似文献   

亚砷酸氧化菌Sinorhizobium sp. GW3的鉴定与亚砷酸氧化酶基因的分离   总被引：1，自引：0，他引：1  

汪耀  涂书新  王革娇 《湖北农业科学》2010,49(1)

亚砷酸氧化细菌能够将毒性大的As(Ⅲ)氧化成毒性小的As(Ⅴ),在生物修复砷污染方面具有应用价值。对一株新型亚砷酸氧化菌Sinorhizobium sp.GW3进行了较全面的鉴定,并分离及分析了亚砷酸氧化酶基因aoxAB。形态学、生理生化鉴定和16S rRNA基因等分析结果表明该菌为Sinorhizobium属。该菌对As(III)抗性的MIC(Minimum inhibitory concentration)为9 mmol/L。首次在Sinorhizobium属中分离了包括编码小亚基aoxA和大亚基aoxB在内的亚砷酸氧化酶基因,其编码的大小亚基与已发现的Agrobacterium tumefaciens(ABB51929)的大小亚基在氨基酸水平上分别有86%、80%的同源性。  相似文献   

不溶性草酸氧化酶的包埋及草酸快速检测方法研究     

胡一鸿  张梦成  许春会 《广东农业科学》2011,38(9):107-109

通过研究不溶性草酸氧化酶包埋方法和草酸快速检测方法,建立氧电极法测定草酸的便捷体系。以大麦麸皮为原料制备不溶性草酸氧化酶,采用5%的琼脂糖包埋,检测包埋酶的稳定性和抗离子干扰能力;以包埋酶为工具酶,通过氧电极法检测草酸含量。试验结果表明:包埋酶连续反应2 h酶活性下降低于5%,贮存30 d活性下降低于8%;草酸检测线性范围0.1～0.8mmol/L,外标法草酸回收率为83%～97%。用氧电极法与显色法测定小麦草酸含量,两种方法的测定值无显著性差异。但氧电极法测定耗时短,反应体系简单,易于推广应用。  相似文献   

Phylogeny of Limenitidinae Butterflies （Lepidoptera： Nymphalidae） Inferred from Mitochondrial Cytochrome Oxidase I Gene Sequences     

ZHANG Min  CAO Tian-wen  ZHONG Yang  GUO Ya-ping  MA En-bo 《中国农业科学(英文版)》2011,10(4):566-575

The phylogenetic analyses of the subfamily Limenitidinae are performed based on 1471 bp of mtDNA cytochrome oxidase subunit I (C01) gene sequence data which were obtained from 21 individuals spanning 9 genera, along with those of 17species obtained from GenBank, using Apatura iris, Aglais urticae, and Polyura dolon as outgroup species. Although the transitions at the third codon positions of the COI data set were highly saturated, they were still retained for analysis as they contain the majority of the phylogenetic information, and thus, the maximum pasimony (MP) under different weighting schemes and maximum likelihood (ML) trees were reconstructed in this study. The results showed that within this subfamily, the results based on the COI gene sequences are approximately identical to the traditional classification results. However, the clustering of Lexias pardalis and Tanaecia julii within the genus Euthalia as well as the clustering of Phaedyma aspasia within the genus Neptis with weak support are different from that of the current classification scheme made by Chinese scholars. The genus Limenitis is splited into two subelusters in the trees constructed by using MP and ML methods. These results support one of the strongest hypotheses for the tribe relationships within Limenitidinae.  相似文献   

海藻酸钠包埋固定胆红素氧化酶的优化     

郑文爽  赵敏 《东北林业大学学报》2011,39(3):127-128

为改善胆红素氧化酶性质,提高酶的使用效率,以海藻酸钠为载体,采用包埋法固定了胆红素氧化酶,并优化了固定化条件,研究了固定化胆红素氧化酶的酶学性质.结果表明:海藻酸钠的质量浓度为0.10 g·mL-1、CaCl,浓度为0.20 mol·L-1、固定时间为1 h、酶的固定化效果最好,固定化酶的平均酶活力回收率达32%,固定...  相似文献   

槲皮素和硫脲对野桑蚕多酚氧化酶的抑制类型     

张永亮  朱勇 《湖北农业科学》2011,(21):4399-4400

多酚氧化酶(Polyphenol oxidase,PPO)在昆虫的变态发育和免疫防御中起着非常重要的作用,为开发以该酶为靶标的新型害虫抑制剂提供理论依据,以野桑蚕(Bombyx mandarina)五龄幼虫为材料提取多酚氧化酶,探讨了槲皮素和硫脲对该酶的抑制作用。结果表明,这两种抑制剂分别属于竞争性和非竞争性抑制剂。  相似文献   

钼对小鼠后代醛氧化酶和黄嘌呤氧化酶转录水平的影响     

张才  王雪莹  李鹏  白刃  陈庭锋  杨自军 《东北农业大学学报》2012,43(6):72-76

试验选用60只二月龄的清洁级ICR小鼠,随机分成4组,每组10只雌性和5只雄性小白鼠,雌雄分开饲养,饮水中加入不同剂量的钼,分别是空白对照组、低钼组(100 mg.L-1)、中钼组(200 mg.L-1)、高钼组(400mg.L-1),4周后雌雄合笼饲养。产仔后从每组仔鼠中随机雌性小白鼠30只和15只雄性小白鼠按原分组给予不同剂量的钼,8周后,再从每组中随机选取雌性小白鼠10只和5只雄性小白鼠合笼饲养,待其产仔。如此往复,使小鼠繁殖四代。以第三代(F2代)作为研究对象,应用实时荧光定量PCR的方法测定肝脏AOX和XO的转录水平。结果表明,与对照组相比F2代小鼠AOX和XO的转录水平随钼添加剂量的增加呈递增趋势,实验组与对照组差异显著(P<0.05)。可见长期钼暴露可提高小鼠后代AOX和XO的转录水平。  相似文献   

黑曲霉A9葡萄糖氧化酶的酶学性质研究   总被引：4，自引：1，他引：4  

王志新  于宏伟  韩军  李宁  林杨  贾英民 《河北农业大学学报》2006,29(4):69-72,83

为研究葡萄糖氧化酶的酶学性质,对黑曲霉A9发酵液提取纯化,酶蛋白达到电泳纯,然后对纯酶进行酶学性质研究。结果表明:葡萄糖氧化酶的分子量为138 200 Da,糖基化程度为2.67%,最适pH为6.7,最适温度为30℃,该酶的pH稳定范围较宽,而且热稳定性很好,Ag+、Hg2+、亚硫酸氢钠对酶有强烈的抑制作用,该酶对葡萄糖的Km值为35.74 mmol/L。该研究表明,黑曲霉葡萄糖氧化酶具有潜在的应用价值,最适合应用于食品、医药及生物等领域。  相似文献   

乙酰辅酶A羧化酶(ACC)的结构与功能     

韩春春  王继文  魏守海 《安徽农业科学》2006,34(3):413-414,416

乙酰辅酶A羧化酶(ACC)催化乙酰辅酶A形成丙二酸单酰辅酶A的羧化作用,是在脂肪酸合成和代谢中起重要作用的中间代谢物。ACC包括ACCα和ACCβ两种形式,被不同的基因编码。ACC(α265 kDa)主要在脂肪生成活跃的肝脏、脂肪组织和乳腺中表达。ACC(β275 kDa)主要在β-氧化作为主要能量来源的骨骼肌和心脏中表达。对ACC的结构特点和基本功能进行了探讨。  相似文献   

Response dynamics of three defense related enzymes in cotton leaves to the interactive stress of Helicoverpa armigera (Hübner) herbivory and omethoate application     

SHA Pin-jie  FAN Yin-jun  WANG Zhi-chao  SHI Xue-yan 《中国农业科学(英文版)》2015,(2):355-364

In order to explore the response dynamics of the activities of defense related enzymes in cotton leaves towards the interactive stress of Helicoverpa armigera herbivory and omethoate application, the activities of phenylalanine ammonia-lyase(PAL), lipoxygenase(LOX), and polyphenol oxidase(PPO) were examined from 6 to 126 h after cotton leaves were treated 12 h of H. armigera herbivory, and then sprayed with 800 mg L–1 omethoate. The results showed that the changes in the activities of PAL, LOX and PPO that occured under the interactive stress of H. armigera herbivory and omethoate application reflected the interactive effects of the two stresses on cotton defense. The similarity between the response dynamics of PAL, LOX, and PPO activities in cotton leaves under the interactive stress and that under H. armigera herbivory treatment alone showed that the induction of H. armigera herbivory on the activities of PAL, LOX and PPO in cotton leaves played a leading role in the interactive effects, and the effect of omethoate application played only a minor role. A joint factor analysis was performed according to a method which has been used to analyze the joint toxicity of pesticides; this analysis sought to clarify if there was a synergistic, antagonistic, or additive effect on PAL, LOX, and PPO activity in cotton leaves resulting from the interactive H. armigera herbivory and omethoate treatment. In the interactive effect on the response of PAL activity in cotton leaves, antagonistic effects of the omethoate application towards H. armigera herbivory were observed at 6 and 12 h. Synergistic effects were then observed at 18 and 30 h. Antagonistic effects were observed from 54 to 78 h and synergistic effects were finally observed at 126 h. The correlation between H. armigera herbivory and omethoate application in the interactive effect on cotton defense responses of LOX activity also fluctuated from synergism to antagonism during the time course. In the interactive effect on PPO activity, only antagonism was observed between H. armigera herbivory and omethoate application. In the interactive stress of H. armigera herbivory and omethoate application on cotton defense responses, omethoate affected the defense responses of cotton to H. armigera herbivory by producing antagonistic and synergistic effects. These results will be useful to understand the relationship between host plant and herbivorous pest.  相似文献