蜂王浆中10-HDA的研究进展     

倪辉  杨远帆  彭莺 《中国蜂业》2006,57(3):9-10

对蜂王浆中10-HDA的来源、结构、性质及功能等方面的研究进展进行了介绍，并对近年来不断改进和完善的几种测定方法，如分光光度法、色谱法、酶联免疫法、气相色谱-质谱法等进行了简要分析。  相似文献   

Recombinant hFGF-10 adenovirus promotes proliferation of kerotinocytes     

WU Xiao-ping  ZENG Yao-ying 《园艺学报》2007,23(2):213-216

AIM:To construct the recombinant adenoviral vector containing human fibroblast growth factor 10 (hFGF-10) gene, and to study the effect of the recombinant adenovirus on the proliferation of kerotinocytes. METHODS:HFGF-10 gene was amplified by PCR and ligated with shuttle vector pAdTrack-CMV to get the recombinant plasmid pAdTrack-CMV-hFGF-10, which was linearized with PmeI and transferred into Escherichia coli BJ5183 containing the adenoviral bone plasmid pAdEasy-1 for homologous recombination to obtain the recombinant adenoviral plasmid pAdEasy-hFGF-10. The recombinant adenoviral plasmid was then transfected into HEK-293 cell line to package and amplify the recombinant adenovirus. The expression of hFGF-10 in HaCat cells infected with the recombinant adenovirus was detected by Western blotting. The influence of the recombinant adenovirus on the proliferation of kerotinocytes was checked by MTT. RESULTS:The recombinant adenovirus containing hFGF-10 gene was successfully constructed, which effectively infected HaCat cells. The result of Western blotting showed that a protein in culture media of the infected HaCat cells reacted with hFGF-10 antibody. The recombinant adenovirus stimulated the proliferation of kerotinocytes. CONCLUSION:HaCat cells infected with the recombinant adenovirus expresses and secrets hFGF-10 protein, which promotes the proliferation of HaCat cells.  相似文献   

Effects of ganmao shuangjie heji on the inflammatory damage in the lungs of mice infected by IV FM1     

GAO Gui-xin  SHEN Hua-hao 《园艺学报》2007,23(6):1137-1140

AIM: To establish the influenza infected mouse model and study the anti-inflammatory effect of ganmao shuangjie heji. METHODS: IV FM1 infected mice were used as the animal model. The changes of pathology and the cytokine TNF-α, IFN-γ and IL-10 in the lung were observed by HE staining and ELISA (double antibody sandwich enzyme linked immunosorbent assay) after ganmao shuangjie heji treatment. RESULTS: After infected by influenza virus, severe interstitial pneumonia was induced in the model group. Mild interstitial pneumonia was observed in ganmao shuangjie heji treated group. The protein expressions of cytokine TNF-α, IFN-γ and IL-10 were higher in model group than those in the control group. The protein expressions of TNF-α and IFN-γ in ganmao shuangjie heji treated group decreased and IL-10 expression increased significantly compared with model group. CONCLUSION: Ganmao shuangjie heji decreases the expressions of TNF-α and IFN-γ, and increases the expression of IL-10, thus, alleviates inflammatory injury. The clinical application of this medicine can shorten the course of disease.  相似文献   

菜用大青豆AC10的选育     

刘学华  曹亚洲 《长江蔬菜》1998,(9):34-35

ＡＣ１０大豆是以７５－２３－１－５－１５品系经激光红宝石辐照,选衣出７５－５４红品系,以之为父本,以西德青豆ＳｏｇｌｅｅｎＯｇｄｅｎ为线本进行有性杂交,再用红宝石２５００Ｖ辐射选能而成,具早熟、高产、优质等特点,６６７ｍ＾２产量１５４．４７ｋｇ,比对照八个月爆增产３０．６６％。  相似文献   

中椒10号的选育     

杨桂梅  张宝玺 《中国蔬菜》1998,1(5):11-0

中椒１０号系以９００１３－３和７８－１－１两个自交系配制而成的微辣型一代杂种,熟性早,早期产量比对照品种增加２．４％～１２．５７％,结果性好,抗ＴＭＶ,中抗ＣＭＶ。一般每６６７ｍ２产２５００～３５００ｋｇ,平均单果重３０．９～４２．５ｇ,果实长羊角形,果面光滑,颜色绿,肉质脆嫩,味微辣,品质佳,果实商品率高。已在北京、天津、河北、东北、安徽、四川及广东、广西、海南等南菜北运基地推广,面积达４８００ｈｍ２。  相似文献   

Gene expression of fatty acid-binding proteins, fatty acid transport proteins (cd36 and FATP) and β-oxidation-related genes in Atlantic salmon (Salmo salar L.) fed fish oil or vegetable oil     

B.E. TORSTENSEN  D.A. NANTON  P.A. OLSVIK  H. SUNDVOLD  & I. STUBHAUG 《Aquaculture Nutrition》2009,15(4):440-451

Relative gene expression pattern of fatty acid transport proteins (FATP and cd36), intracellular fatty acid-binding proteins (FABP3, FABP10 and FABP11), β-oxidation-related genes [carnitine palmitoyl transferase II (CPTII), peroxisome proliferator-activated receptor β (PPARβ), acyl-CoA oxidase (AOX), long-chain fatty acyl-CoA synthetase (FACS), acyl-CoA dehydrogenase (dehydrogenase)] and uncoupling protein 2 (UCP2) was assessed by RT-qPCR in Atlantic salmon muscle (red and white), liver, heart, myosepta and visceral fat. FABP11, a FABP isoform not previously described in Atlantic salmon, was highly expressed in visceral fat and myosepta and at the lower level in red muscle, white muscle, myosepta and heart. Furthermore, Atlantic salmon were fed either a diet containing fish oil (FO) or a complete replacement of FO with a vegetable oil blend (55% rapeseed oil, 30% palm oil and 15% linseed oil; VO) for the production cycle (27 months from start of feeding and until ∼4.5 kg mean weight). The expression of genes related to β-oxidation, fatty acid uptake and transport in the white muscle indicate ( n  = 3) significant down-regulation in VO fed Atlantic salmon and correlated with previously reported white muscle triacylglycerol stores and β-oxidation. FABP11 in visceral fat and myosepta was also down-regulated in VO fed fish.  相似文献   

Effect of F10 gene on activities of transcriptional factor NF-κB and AP1     

CAO Xiao-min  PANG Zhan-jun  QUAN Song  XING Fu-qi 《园艺学报》2008,24(5):901-904

  相似文献   

SRSF10对山羊肌内前体脂肪细胞分化的影响     

刘珂含  王永  李艳艳  王重洋  朱江江  熊燕  李安  林亚秋 《畜牧兽医学报》2022,53(6):1768-1778

旨在克隆山羊SRSF10基因序列,明确其生物学特性,并通过过表达和干扰手段阐明SRSF10对山羊肌内脂肪细胞分化的影响。本研究以简州大耳羊(Capra hircus)为试验对象,利用RT-PCR技术克隆山羊SRSF10基因序列,并进行生物信息学分析; 利用实时荧光定量PCR(real-time quantitative PCR,qPCR)技术研究其在成脂诱导分化不同阶段细胞中的表达水平; 转染pcDNA3.1-SRSF10过表达载体和SRSF10-siRNA至山羊肌内前体脂肪细胞并诱导分化,通过油红O和Bodipy染色法从形态学上明确其对脂滴积聚的影响; 通过qPCR方法检测脂肪细胞分化标志基因表达的变化。结果显示,获得山羊SRSF10基因序列为1 026 bp,其中CDS区552 bp,共编码183个氨基酸; SRSF10在诱导分化96 h的肌内脂肪细胞中表达量最高; 过表达和干扰山羊SRSF10分别促进和抑制了肌内脂肪细胞中的脂滴积聚; 过表达后基因SREBP1、PPARγ和C/EBPα的相对表达量极显著上调(P < 0.01),干扰后分化标志基因SREBP1、PPARγ和C/EBPα相对表达水平极显著降低(P < 0.01)。结果表明,山羊SRSF10是肌内脂肪细胞分化的正调控作用因子,并且这种调控作用可能主要通过调节SREBP1、PPARγ和C/EBPα的表达来实现。  相似文献   

A KCNJ10 mutation previously identified in the Russell group of terriers also occurs in Smooth-Haired Fox Terriers with hereditary ataxia and in related breeds     

Cecilia Rohdin  Douglas Gilliam  Caroline A. O’Leary  Dennis P. O’Brien  Joan R. Coates  Gary S. Johnson  Karin Hultin J?derlund 《Acta veterinaria Scandinavica》2015,57(1)

Background

Hereditary ataxias with similar phenotypes were reported in the Smooth-Haired Fox Terrier, the Jack Russell Terrier and the Parson Russell Terrier. However, segregation analyses showed differing inheritance modes in these breeds. Recently, molecular genetic studies on the Russell group of terriers found independent mutations in KCNJ10 and CAPN1, each associated with a specific clinical subtype of inherited ataxia. The aim of this study was to clarify whether or not Smooth-Haired Fox Terriers with hereditary ataxia and dogs of other related breeds harbor either of the same mutations. A sub goal was to update the results of KCNJ10 genotyping in Russell group terriers.

Findings

Three Smooth-Haired Fox Terriers with hereditary ataxia and two Toy Fox Terriers with a similar phenotype were all homozygous for the KCNJ10 mutation. The same mutation was also found in a heterozygous state in clinically unaffected Tenterfield Terriers (n = 5) and, in agreement with previous studies, in Jack Russell Terriers, Parson Russell Terriers, and Russell Terriers.

Conclusions

A KCNJ10 mutation, previously associated with an autosomal recessive spinocerebellar ataxia in Jack Russell Terriers, Parson Russell Terriers, and Russell Terriers segregates in at least three more breeds descended from British hunting terriers. Ataxic members of two of these breeds, the Smooth-Haired Fox Terrier and the Toy Fox Terrier, were homozygous for the mutation, strengthening the likelihood that this genetic defect is indeed the causative mutation for the disease known as “hereditary ataxia” in Fox Terriers and “spinocerebellar ataxia with myokymia, seizures or both” in the Russell group of terriers.  相似文献   

黄土丘陵区白羊草与达乌里胡枝子混播草地土壤呼吸日变化特征   总被引：1，自引：0，他引：1  

陈吉  王京  高志娟  郭亚力  吴爱姣  徐炳成 《草业科学》2015,32(1):19-27

土壤呼吸是反映土壤质量和肥力性状的重要指标,阐明禾―豆混播草地土壤呼吸作用的变化规律及其影响因素,可以为准确评估混播草地的环境效应及响应提供依据。在2011和2012年6月份,采用动态密闭气室分析法,比较了白羊草(Bothriochloa ischaemum)与达乌里胡枝子(Lespedeza davurica)不同比例间作混播草地总土壤呼吸速率的日变化特征、混播植物贡献率以及温度敏感性等。结果表明,不同混播比例草地的总土壤呼吸速率日变化为单峰型曲线,峰值出现在12:00~14:00。总土壤呼吸速率日均值高低顺序为单播白羊草白羊草和达乌里胡枝子间作单播达乌里胡枝子。不同间作比例草地的总土壤呼吸速率与白昼气温相关显著(P0.05),而与5、10和15 cm土层白昼土壤温度关系不明显;单播条件下,白羊草草丛的土壤呼吸对温度的敏感性高于达乌里胡枝子;各草地土壤呼吸Q10值的变化范围为1.48~2.61,以单播白羊草最高,单播达乌里胡枝子和两者间作混播草地间无显著差异(P0.05)。  相似文献