DVM students report higher psychological distress than the Australian public,medical students,junior medical officers and practicing veterinarians     

HH Yang  MP Ward  A Fawcett 《Australian veterinary journal》2019,97(10):373-381

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S100a10基因对小鼠前体脂肪细胞白色和棕色成脂分化的影响   总被引：1，自引：1，他引：0  

任玲  胡鑫  邢义珅  王亚慧  李俊雅  张路培 《畜牧兽医学报》2019,(6):1171-1178

旨在用小鼠模型探究全基因组关联分析(GWAS)筛选到的西门塔尔牛大理石花纹评分性状正相关基因S100钙结合蛋白A10( S100A10 )对小鼠前体脂肪细胞成脂分化的影响。本研究以来源于C57BL/6品系小鼠腹股沟两侧白色脂肪组织的前体脂肪细胞为材料,体外进行白色/棕色成脂分化。通过siRNA干扰 S100a10 基因表达,通过油红O染色检测前体脂肪细胞分化效果,通过荧光定量PCR(qRT-PCR)、蛋白免疫印迹和细胞免疫荧光检测基因和蛋白表达变化。结果显示,敲低 S100a10 后,通过油红O染色发现脂滴明显变少;通过qRT-PCR检测白色成脂分化标志基因 Pparg 、 C/ebpa 、 Fabp4 、 Glut4 、 Adiponectin 、 Leptin 表达量显著下降( P <0.01);棕色成脂分化基因 Ucp1、Pgc1a、Fabp4、Elovl3、Cox7a 表达量显著下降( P <0.01)。敲低 S100a10 后,通过蛋白免疫印迹检测发现,FABP4和PPARG在白色成脂分化中表达量显著下降( P <0.01),通过细胞免疫荧光检测发现FABP4在白色成脂分化中表达量下降;敲低 S100a10 后通过蛋白免疫印迹和细胞免疫荧光检测发现,UCP1和FABP4在棕色成脂分化中表达量显著下降( P <0.01)。综上表明,敲低 S100a10 基因后抑制小鼠前体脂肪细胞的白色/棕色成脂分化,本研究将为探究 S100A10 基因对于肉牛肌内脂肪沉积的影响提供科学依据。  相似文献   

Trans10, cis12 conjugated linoleic acid increases triacylglycerol accumulation in goat mammary epithelial cells in vitro          下载免费PDF全文

Tianying Zhang  Yue Ma  Hui Wang  Juan J Loor  Huifen Xu  Huaiping Shi  Jun Luo 《Animal Science Journal》2018,89(2):432-440

Trans10, cis12 conjugated linoleic acid (t10c12‐CLA) is well‐established in decreasing milk fat content and causing milk fat depression (MFD) in dairy cattle and goats. However, the detailed mechanisms of its effect are not completely understood. Therefore, we used goat mammary epithelial cells (GMECs) to further study the molecular mechanisms whereby t10c12‐CLA regulates milk fat synthesis. The optimal concentration of t10c12‐CLA (100 μmol/L) for cell culture was determined through a cell vitality and morphology assay, and evaluation of abundance of apoptosis‐related proteins. Oil red O stain indicated that t10c12‐CLA increased concentration of cytoplasmic lipid droplets. Furthermore, t10c12‐CLA increased the intracellular triacylglycerol (TG) content (P < 0.05). Among 16 genes related to lipid metabolism that were measured by quantitative real‐time PCR, t10c12‐CLA down‐regulated (P < 0.05) genes involved in de novo fatty acid synthesis including FASN, ACACA and SCD1, and also down‐regulated the protein expression of FASN and SCD1 but up‐regulated (P < 0.05) the expression of CD36 and ADRP. Overall, the data indicate that a side effect of de novo fatty acid synthesis inhibition by t10c12‐CLA is the up‐regulation of fatty acid uptake and accumulation of lipid droplets in GMECs. The biologic reason for such an effect merits further study.  相似文献   

Molecular simulation and experimental study on role of BRAF in anti-melanoma effect of 10-gingerol     

ZHANG Jing-zhi  DENG Bo  JIANG Xiao-li  ZHANG Shuang-wei  CAI Min  LIU Bin 《园艺学报》2018,34(12):2166-2171

AIM:To investigate the role of BRAF protein in the anti-melanoma effect of 10-gingerol (10-G) by molecular simulation techniques and cell experiments. METHODS:Human skin melanoma A375 cells were induced by 10-G (10, 20 and 40 μmol/L) for 24 h. The cell viability was measured by MTS assay and cell counting. The interaction between 10-G and BRAF protein was analyzed by molecular docking and molecular dynamics simulations. The protein levels of p-BRAF, BRAF, p-MEK1/2, p-ERK1/2 and p-P38 were determined by Western blot. RESULTS:Treatment with 10-G significantly reduced the cell viability and cell number in a dose-dependent manner (P<0.01). The binding energy between 10-G and wild-type BRAF was -7.358 kcal/mol, and that between 10-G and V600E mutant BRAF (BRAF^V600E) was -8.255 kcal/mol. Molecular dynamics simulations confirmed that the binding between BRAF^V600E and 10-G was stable. The protein levels of p-BRAF, p-MEK1/2 and p-P38 in the A375 cells was significantly reduced by 10-G (P<0.01), while the protein level of p-ERK1/2 was unchanged. CONCLUSION:The viability of melanoma cells is significantly inhibited by 10-G. The mechanism might be related to the inhibition of BRAF activation.  相似文献   

The molecular mechanism of tolegenic dendritic cells induced by IL-10 in mouse     

LIU Zheng-rong  ZHANG Min  LI Wei-ming  ZHOU Hao  ZOU Ping 《园艺学报》2008,24(2):374-378

AIM:To investigate the relation of tolerogenic dendritic cells (DC) induced by interleukin-10 (IL-10) and the paired immunoglobin-like receptor (PIR) A and B (PIR-A and PIR-B) in mouse. METHODS:The mouse dendritic cell line, DC2.4 cells were cultured with the IL-10 to develope the IL-10-DC and were stimulated by lipopolysaccharide (LPS) for 48 h to induce the mature dendritic cells (LPS-DC). Special small inference RNA (siRNA) molecule of PIR-B was chemically synthesized and was transfected into IL-10-DC by Lipofectamine 2000 (Si-DC). The expression of PIR A and PIR B on DC2.4 cells were measured by semi-quantitative RT-PCR and flow cytometry (FCM). The allogeneic stimulating capacity of DC was measured by mixed lymphocyte reaction (MLR) using ［3H］-thymidine incorporation test. The concentration of IFN-γ in supernatants of MLR from distinct groups was analyzed by ELISA. RESULTS:IL-10 up-regulated the PIR-B and down-regulated the PIR-A by semi-quantitative RT-PCR. On the contrary, LPS down-regulated the PIR-B expression and up-regulated the PIR-A expression. The expression of PIR, which is the common extra-membrane of PIR-A and PIR-B, was increased in both the IL-10-DC and the LPS-DC groups by FCM detection, but the higher expression was found in IL-10-DC group than that in LPS group. The IL-10 induced the higher PIR-B expression, inhibited allogenetic T cell proliferation and down-regulated the IFN-γ secretion. Special siRNA molecules of PIR-B in IL-10 group promoted the T cell proliferation and enhanced the IFN-γ secretion in MLR. CONCLUSION:IL-10 up-regulates the PIR-B expression and makes DC tolerance. Up-regulated PIR-B expression may be the molecular mechanism of tolerogenic dendritic cells induced by IL-10 in mouse.  相似文献   

春大棚黄瓜新品种津优10号的选育     

哈玉洁  王全  杨瑞环  刘殿林 《中国蔬菜》2001,1(5):21-23

津优10号黄瓜以本所优良自交系81为母本,以荷兰黄瓜与中国黄瓜杂交后代经多代自交获得的自交系48为父本杂交而成.生长势强,第1雌花节位在第4节左右,前期以主蔓结瓜为主,早熟,坐瓜率高,瓜条顺直,亮绿色,中等刺瘤,瓜长36cm,横径3cm,单瓜质量160g,抗黄瓜霜霉病、白粉病、枯萎病能力强,适合春秋大棚栽培,每667m2可达5500kg,目前已在天津、河南、河北、山东、内蒙等地示范推广.  相似文献   

红外加热对大豆生态系统CO_2排放的影响     

胡正华  崔海羚  周迎平  陈书涛  申双和 《中国农业气象》2013,(6):636-641

通过田间试验,采用静态箱-气相色谱法测定CO2排放通量,研究红外加热增加叶面温度对土壤、大豆-土壤系统CO2排放的影响。结果表明,红外加热叶面增温2℃促进了土壤CO2的排放,在鼓粒-成熟期对照与增温的排放通量分别为202.09±28.75、378.34±156.17mg·m-2·h-1,增温处理使CO2排放通量增加了87.21%,但未达到显著水平;增温使土壤CO2累积排放量显著增加了39.96%。对照和增温的大豆-土壤系统呼吸的气温敏感性系数Q10值分别为0.68和2.54,土壤呼吸的土壤温度Q10值分别为4.22和1.68。研究表明,增温能促进土壤CO2排放,增加大豆-土壤系统呼吸的Q10值,降低土壤呼吸的Q10值。研究结果可为气候变化条件下估算区域农田温室气体排放提供一定的科学依据。  相似文献   

黄瓜生长素响应因子CsARF10亚家族3个基因的克隆与表达分析     

郭勤卫  李季  崔利  张停林  陈劲枫 《园艺学报》2013,40(6):1071

 利通过蛋白序列同源比对和PCR技术从黄瓜中克隆获得了3条生长素响应因子ARF10基因序列,分别命名为CsARF10a、CsARF10b和CsARF10c。系统发育进化树分析发现CsARF10a与番茄SlARF10基因进化距离较近,而CsARF10b和CsARF10c与拟南芥AtARF10相似性更高;启动子分析显示CsARF10家族基因具有多样化的激素应答元件,荧光定量PCR结果也证明黄瓜幼苗在不同NAA浓度处理以及其他激素的处理下,CsARF10家族基因呈现不同的表达模式;荧光定量PCR结果还显示CsARF10家族基因在黄瓜幼苗的根、茎、叶及雄花中都具有较高的转录水平;CsARF10a、b、c在黄瓜果实发育的早期具有相同的表达趋势,但CsARF10a的转录水平较高,而CsARF10b和CsARF10c表达量较低。  相似文献   

Effect of hay dust extract and cyathostomin antigen stimulation on cytokine expression by PBMC in horses with recurrent airway obstruction     

Simone Lanz  Vincent Gerber  Eliane Marti  Helen Rettmer  Jolanta Klukowska-Rötzler  Bruno Gottstein  Jacqueline B. Matthews  Scott Pirie  Eman Hamza 《Veterinary immunology and immunopathology》2013

Equine recurrent airway obstruction (RAO) is an inflammatory, obstructive airway disease induced by exposure of susceptible horses to inhaled organic dust particles. The immunological process underlying RAO is still unclear. Previous studies have shown that RAO is linked to the Interleukin-4 receptor (IL-4R) gene in one Warmblood family (F1), but not in another (F2). It has also been shown that in F1, but not in F2, RAO is associated with resistance against parasites, suggesting that this association may have an immuno-genetic basis. Therefore, we hypothesized that the T helper (h)1/Th2/regulatory (Treg) cytokine profiles of RAO-associated antigen- and parasite-antigen-stimulated peripheral blood mononuclear cells (PBMC) differ between RAO-affected and healthy horses depending on their genetic background. In our study, PBMC from 17 RAO-affected and 14 healthy control horses of F1 and F2 were stimulated for 24 h with antigens relevant to RAO [hay dust extract (HDE), Aspergillus fumigatus extract (AFE) and lipopolysaccharids (LPS)]; cyathostomin extract (CE) and recombinant cyathostomin antigen (RCA) or with concanavalin A (ConA). Total mRNA levels of IL-4, IL-4R, IL-13, interferon (INF)-γ and IL-10 were examined by qRT-PCR. Stimulation with either HDE or RCA resulted in significant differences in IL-4R mRNA levels between RAO-affected and control horses in F1, but not in F2. For IL-10 mRNA expression, a significant difference between RAO-affected and control horses in F1 but not in F2 was observed only following stimulation with HDE. In contrast to HDE, stimulation with CE resulted in a significant difference of IL-10 mRNA expression level between RAO-affected horses of F2 and healthy horses of F1. No significant differences were detected upon stimulation with any of the other challenge agents. These findings indicate that the immunological response, specifically IL-4R expression, in response to hay dust and cyathostomin antigens, differs between RAO-affected and healthy horses depending on their genetic background. This study shows that analysis of PBMC reveals systemic changes associated with RAO and helps to elucidate immunological pathways involved in this disease.  相似文献   

大豆新品种“延农10号”选育报告   总被引：1，自引：1，他引：0  

宋昌益  姜青石  秦元利  张基德  黄初女 《延边大学农学学报》2002,24(3):162-165

“延农10号”是1991年延边农业科学研究院旱田研究所以合丰25号为母本，以绥农8号为父本进行有性杂交经多年培育而成的，该品种需≥10℃积温2250℃左右，1997－2001年在各级产量试验中均表现早熟、高产、优势、多抗；2002年2月通过吉林省农作物品种审定委员会审定命名，适宜吉林省高寒山区、半山区早熟地区种植，种植密度一般为24－30万株／hm^2。  相似文献