Effect of inclusion of cashew globulin (Anacardium occidentale) to a casein diet on lipid parameters in rats     

Prabha SP  Rajamohan T 《Plant foods for human nutrition (Dordrecht, Netherlands)》1998,53(1):83-92

The effect of inclusion of cashew globulin to a casein diet on lipid metabolism was studied in rats fed diets with two levels of cashew globulin meal. Inclusion of cashew globulin to a casein diet produced lower levels of total cholesterol, triacylglycerol and phospholipids in the serum and tissues and lower levels of serum lipoprotein cholesterol. There was decreased cholesterogenesis in the liver as evidenced by decreased activity of HMG CoA reductase and decreased release of lipoproteins into circulation. Rats fed cashew globulin along with casein also showed higher activity of LPL in the heart and adipose tissue and higher activity of LCAT. Increased hepatic diversion of cholesterol to bile acid synthesis and increased excretion of bile acids and sterols were also observed in these groups. Activity of glucose-6-phosphate dehydrogenase and malic enzyme was decreased in rats fed cashew globulin along with casein. This study demonstrates that cashew globulins included in the diet of rats are able to alter lipid metabolism which results in lower levels of lipid parameters in the serum and tissues.  相似文献   

Cottonseed protein: What does the future hold?     

Betty B. Alford  George U. Liepa  Anne D. Vanbeber 《Plant foods for human nutrition (Dordrecht, Netherlands)》1996,49(1):1-11

Cottonseed protein has the potential to increase the world's food supply while decreasing the incidence of malnutrition among the world's hungry. Nutritionally, cottonseed flour compares favorably to other animal and vegetable protein sources, as it is low in fat and contains a substantial amount of high biological value protein. Animal studies, as well as human research, using gossypol-free glandless cottonseed flour have shown that cottonseed protein promotes growth, increased weight gain, and a positive nitrogen balance. Cottonseed protein food products have been shown to be a healthy addition to the diets of children, college-age women, and the elderly. With its light color and bland flavor, cottonseed has many uses in the food processing arena. Baked goods, snack foods and candy, as well as pet and livestock feed are just a few successful products developed utilizing cottonseed protein.  相似文献   

Hypolipidemic effect of coriander seeds (Coriandrum sativum): mechanism of action     

Chithra  V.  Leelamma  S. 《Plant foods for human nutrition (Dordrecht, Netherlands)》1997,51(2):167-172

The effect of the administration of coriander seeds (Coriandrum sativum) on the metabolism of lipids was studied in rats fed a high fat diet with added cholesterol. The spice had a significant hypolipidemic action. The levels of total cholesterol and triglycerides decreased significantly in the tissues of the animals of the experimental group which received coriander seeds. Significant increases in -hydroxy, -methyl glutaryl CoA reductase and plasma lecithin cholesterol acyl transferase activity were noted in the experimental group. The level of LDL + VLDL cholesterol decreased while that of HDL cholesterol increased in the experimental group compared to the control group. The increased activity of plasma LCAT, enhanced hepatic bile acid synthesis and the increased degradation of cholesterol to fecal bile acids and neutral sterols appeared to account for its hypocholesterolemic effect.  相似文献   

Effect of boiled barley-rice-feeding in hypercholesterolemic and normolipemic subjects     

Sachie Ikegami  Masako Tomita  Setsuko Honda  Momoko Yamaguchi  Reiko Mizukawa  Yoko Suzuki  Keiko Ishii  Saeko Ohsawa  Nanako Kiyooka  Mitsuru Higuchi  Shuhei Kobayashi 《Plant foods for human nutrition (Dordrecht, Netherlands)》1996,49(4):317-328

Barley contains approximately 10% dietary fiber and is easily cooked with rice, the dominant cereal in Japan, to increase the intake of dietary fiber. This research involved three experiments to examine the influence of barley on blood lipids in human subjects. All subjects received a boiled barley-rice (50/50 w/w mix) supplement two times per day in place of rice for 2 or 4 weeks. In the normolipemic subjects, serum lipids were unaffected by the ingestion of barley for 4 weeks. In twenty hypercholesterolemic men aged 41 ± 5 years, the ingestion of barley was associated with a significant fall in serum total cholesterol, LDL-cholesterol, phospholipids and LDL and VLDL-lipoproteins. In seven mildly hypercholesterolemic women aged 56±7 years, a significant improvement of serum lipid profiles was observed. The present study suggests the possibility that the ingestion of barley-rice could lower serum lipids in hypercholesterolemic subjects.  相似文献   

卵黄和低密度脂蛋白对冷冻保存绵羊精子胆固醇、磷脂含量的影响   总被引：3，自引：0，他引：3  

郑云胜  宏伟  倪利平  田东海  芦永跃  邓常胜  李金泉 《华北农学报》2008,23(6)

为了探索绵羊冷冻精液受精率低的原因,在稀释液中用低密度脂蛋白代替卵黄冷冻绵羊精液,研究LDL对冷冻中精子脂类含量的影响。结果表明,以卵黄稀释液冷冻精液,精子胆固醇含量从鲜精的234．54nmol／10^9降低到163．76nmol／10^9精子,胆固醇与磷脂的比值（c／p）从0．54下降到0．36;以9g／100mL LDL代替卵黄,冷冻后胆固醇含量为243．73nmol／10^9精子,c／p值保持在0．50。研究得出在卵黄稀释液中冷冻绵羊精液,精子胆固醇流失,c／p值下降;以LDL代替卵黄,可有效保护精子胆固醇,避免流失,维持了精子c／p值。  相似文献   

High-cholesterol diet with or without corn oil for establishing atherosclerotic model in rabbits     

WEN Yi  ZHOU Yan  LIU Xiang  ZHANG Nan-rong  WANG Hong-li  LV Bao-feng  JIN San-qing 《园艺学报》2014,30(6):1148-1152

AIM：To compare the reliability and plaque area between using high-cholesterol diet and high-cholesterol diet with corn oil to establish a rabbit atherosclerotic model. METHODS：Eighteen New Zealand rabbits were randomly divided into 3 groups (6 rabbits each): normal diet group (group C), high-cholesterol diet group (group H1) and high-cholesterol diet containing 6% corn oil group (group H2). All rabbits were fed for 12 weeks, and their body mea-sured was weighed at the end of every weeks. The serum levels of high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC) and triglyceride (TG) were measured by automatic chemistry analyzer at 0 week and 12 weeks. At the end of 12 weeks, the thoracic aorta of 8-cm length since aortic root was isolated from the rabbit after anesthesia, and stained with Sudan IV or oil red O to verify the existence of plaque. The percentage of plaque area (PA/IA) in the intima area was further calculated by ImageJ2x software. RESULTS：At the end of 12-week feeding, the serum levels of HDL-C, LDL-C and TC in both group H1 and group H2 were significantly higher than those in group C, and serum TG in group H2 was significantly higher than that in group C. Serum HDL-C in group H2 was significantly higher than that in group H1, but no significant difference of serum LDL-C, TC and TG between group H1 and group H2 was found. There was no plaque in the intima in group C, and plaques were observed in the intima of all rabbits in group H1 and group H2. Rabbit atherosclerotic models in both group H1 and group H2 were established with a success rate of 100%. The values of PA/IA in group H1 ［(49.74±18.78)%］ and group H2 ［(56.95±26.74)%］ were both significantly higher than that in group C (0%), and no significant difference of PA/IA between group H1 and group H2 was observed. CONCLUSION：High-cholesterol diet with or without corn oil can establish a rabbit atherosclerotic model with a success rate of 100% after 12-week feeding, and the percentage of plaque area in the total aortic intimal area is not different in the 2 feeding methods.  相似文献   

Effect of puerarin on cholesterol influx and efflux in RAW264.7-treated foam cells     

WEN Zhu-hua  FU Da-wei  WANG Jing  XU Ru-qing  WU Gong-xiong  LIU Shi-ming 《园艺学报》2016,32(9):1614-1620

AIM: To study the protective effect of puerarin on the atherosclerosis of RAW264.7-derived foam cells. METHODS: The model of foam cells was established by incubating the RAW264.7 cells with ox-LDL. The cholesterol uptake was evaluated by a DiI-ox-LDL binding assay. The ability of cholesterol efflux of the RAW264.7-derived foam cells was detected by cholesterol efflux assay. The protein levels of LC3Ⅱ, P62, CD36, ABCA1, LAL and p-AMPK were determined by Western blot. RESULTS: Puerarin treatment reduced the cholesterol uptake capacity and enhanced the cholesterol efflux rate. The protein levels of LC3Ⅱ, ABCA1 and LAL in puerarin group were higher than that in ox-LDL group, while the protein levels of P62 and CD36 were obviously decreased, and those in rapamycin treatment group had the same change as puerarin group. The protein levels of LC3Ⅱ, ABCA1 and LAL were obviously decreased and the protein level of p-AMPK was increased after co-treated with 3-MA. CONCLUSION: Puerarin promotes LAL and ABCA1-mediated cholesterol efflux in ox-LDL-treated RAW264.7 macrophages, which might enhance autophagy through AMPK-dependent pathway for cholesterol efflux regulation, and reduce the uptake of lipids by CD36 negative regulation.  相似文献   

Comparison of 2 electrophoretic methods and a wet‐chemistry method in the analysis of canine lipoproteins          下载免费PDF全文

Erica Behling‐Kelly 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(1):124-134

  相似文献   

Association of rs2162459 polymorphism in CYP7A1 gene with effectiveness of atorvastatin in northern Chinese Han population     

WANG Guo-chang  CHEN Xiao-ming  LI Shu-yuan  ZHANG Na-na  ZHENG Li-juan  CHEN Tian-tian  ZHUANG Mao-qiang  ZHANG Lin  CHEN Huan-qin  L Ming 《园艺学报》2013,29(8):1365-1369

AIM：To investigate the association of the polymorphism of rs2162459 locus in cytochrome P-450, family 7, subfamily A, polypeptide 1 (CYP7A1) gene, encoding cholesterol 7α-hydroxylase, with the effectiveness of atorvastatin in northern Chinese Han population. METHODS：Clinical data and blood samples of 200 cases of hyperlipidemia patients were collected. The variants of rs2162459 in CYP7A1 gene were detected by multiplex SNaPshot technology. Several genetic models were constructed to analyse the association of gene polymorphism with the effectiveness of atorvastatin by logistic regression method. RESULTS：The polymorphism of rs2162459 was consistent with Hardy-Weinberg equilibrium. The baseline high-density lipoprotein cholesterol (HDL-C) concentrations in three genotypes of AA, GA and GG were (136±0.94) mmol/L, (1.16±0.38) mmol/L and (1.07±0.28) mmol/L, respectively (P<0.05). There were differences in the genotypic frequency and allelic frequency between atorvastatin effective and ineffective groups (both P<005). Significant differences in regulating HDL-C level among the three genotypes of rs2162459 were found after logistic regression. The results of additive model, generalized model and dominant model, presented as OR (95% CI), were 1.74 (1.09~2.77), 2.86 (1.13~7.25) and 2.21 (1.12~4.33), respectively. CONCLUSION: The baseline HDL-C level in the carriers of GG genotype is lower than that in the carriers of the other two genotypes. The HDL-C-elevating effect of atorvastatin on GG genotype carriers is more significant than that on AA genotype carriers.  相似文献   

腺病毒介导的秦川牛固醇调节元件结合蛋白2基因(SREBP2)过表达载体的构建与鉴定     

付常振  刘扬  昝林森  王虹  成功  王嘉力 《农业生物技术学报》2013,21(8):993-1001

固醇调节元件结合蛋白2（sterol regulatory element binding protein 2,SREBP2）属于碱性螺旋-环-螺旋亮氨酸拉链转录因子家族,在胆固醇的代谢过程中具有重要的调控作用。克隆秦川牛（Bos taurus）的SREBP2基因并构建相应腺病毒过表达载体,包装扩繁获得高滴度腺病毒,对于在细胞水平上研究SREBP2基因的功能和作用机制具有重要作用。本研究以秦川牛脂肪组织为实验材料,提取总RNA并反转录为cDNA,依据GenBank收录的牛的SREBP2基因（Accession No.NM₀01205600）mRNA序列设计引物,克隆出SREBP2基因编码区（coding sequence,CDS）全长。将SREBP2基因与穿梭载体连接构建pAdTrack-CMV-SREBP2表达载体,用PmeⅠ分别酶切线性化pAdTrack-CMV-SREBP2和空白对照pAdTrack-CMV载体并转化含有骨架载体pAdEasy-1的大肠杆菌（Escherichia coli）BJ5183感受态进行同源重组,得到腺病毒重组载体pAd-SREBP2和pAd-CMV,分别用PacⅠ酶切后胶回收DNA大片段,并将回收产物转染293A细胞包装得到腺病毒Ad-SREBP2和Ad-CMV,增殖并提高病毒滴度,得到高滴度病毒后,用绿色荧光蛋白（green fluorescent protein,GFP）标记法测定显示Ad-SREBP2和Ad-CMV的病毒滴度分别为7×108和1.3×109GFU/mL。将Ad-SREBP2和Ad-CMV腺病毒侵染秦川牛前体脂肪细胞检测病毒的有效性,实时定量PCR检测结果显示,侵染48h时SREBP2基因的表达量提高了102.3倍。本研究成功克隆了秦川牛的SREBP2基因,构建了病毒重组体,包装增殖获得了高滴度病毒,为在细胞水平上基因功能的研究提供了基础资料。  相似文献