胆固醇氧化酶基因的克隆及其在大肠杆菌中的表达     

银国利  张锐  郭三堆  陈毓荃  牛天贵 《西北农业学报》2003,12(3):12-16,35

采用PCR方法从来源于蒙古野驴的马红球菌(Rhodococcus equi)4-2G2菌株中分离出编码胆固醇氧化酶的新基因(choEW),其与已知的胆固醇氧化酶基因choE和choB同源性均达99％;按其推断的氨基酸序列与choE和choB相比在485位缺少一个氨基酸,分析认为choEW与choB和choE属于同一基因家族。将choEW插入到原核表达载体pET-His中,构建出重组质粒pETW,并转化Escherichia coli BL21(DE3)plysS获得工程菌。经IPTG诱导后表达出分子量约为56 kD的蛋白质,与核苷酸序列推断的蛋白大小相符。目的蛋白表达量约占细胞总蛋白的11.9％。  相似文献   

胆固醇氧化物分析方法研究进展     

方益  赵凯  周勇  赵巧灵 《安徽农业科学》2015,(2)

介绍了胆固醇氧化物种类,综述目前国内外对胆固醇氧化物检测方法:包括油脂提取、胆固醇氧化物纯化、富集,及仪器分析方法对其定性、定量检测  相似文献   

Impact of mucin,bile salts and cholesterol on the virulence of Vibrio anguillarum towards gnotobiotic sea bass (Dicentrarchus labrax) larvae     

《Veterinary microbiology》2015,175(1):44-49

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丝素的食用研究   总被引：3，自引：3，他引：3  

刘朝良 《安徽农业大学学报》1995,22(2):173-176

叙述了从废丝中提取丝素蛋白的工艺流程及理论指标的测试方法,分析了丝素蛋白的组成,并做了小白鼠添食试验,结果表明：丝素蛋白为阶氨基酸,几乎具备人体必需的氨基酸,其含量约占氨基酸总量的１０％左右,理化指标符合营养食品要求;在解酒,降低胆固醇等方面效果明显。  相似文献   

Serum and liver lipids in rats fed diets containing corn oil,cottonseed oil,or a mixture of corn and cottonseed oils     

Radcliffe JD  King CC  Czajka-Narins DM  Imrhan V 《Plant foods for human nutrition (Dordrecht, Netherlands)》2001,56(1):51-60

Previous research has demonstrated that the total replacement of corn oil (CO) with cottonseed oil (CSO) in the diets of growing male rats results in a lowering of the total cholesterol (TC) concentration in the serum. It is not, however, known if a partial replacement of dietary CO with CSO would be sufficient to lower TC levels, nor is the effect of replacing dietary CO with CSO on other indices of cholesterol status (e.g., the levels of high-density lipoprotein cholesterol [HDL-C], non-HDL-C [i.e., very-low and low-density lipoprotein-cholesterol], or the TC/HDL-C ratio). Growing male rats were fed diets having CO, CSO, or a CO/CSO (1:1, w/w) oil mixture (OM) as the source of dietary oil (diet groups CO, CSO, and OM, respectively). There were no differences between CO and OM groups for TC, HDL-C, non-HDL-C, or the TC/HDL-C ratio. However, TC was significantly lower (p 0.05)for the CSO group than the CO group (2.85 vs. 3.23 mmol/l, or 110 vs. 125 mg/dl, respectively) as was HDL-C (1.83 vs. 2.13 mmol/l, or 71 vs. 82 mg/dl, respectively), but there were no differences between these groups for non-HDL-C or the TC/HDL-C ratio. Diet had no effect on serum triglycerides (TGs) or on total liver content of either cholesterol or TGs. Thus, partial (50%) replacement of CO with CSO was without effect on any of these serum indices of cholesterol status, but total replacement resulted in lower TC and HDL-C, without affecting non-HDL-C or the TC/HDL-C ratio. Because, relative to CO, CSO has higher levels of saturated fatty acids but lower levels of monounsaturated and polyunsaturated fatty acids, the cholesterol-lowering effect of CSO appears to be unrelated to its fatty acid composition, and may be caused by a component of its nonsaponifiable fraction.  相似文献   

Effect of hesperetin,a citrus flavonoid,on the liver triacylglycerol content and phosphatidate phosphohydrolase activity in orotic acid-fed rats     

Cha  J.-Y.  Cho  Y.-S.  Kim  I.  Anno  T.  Rahman  S.M.  Yanagita  T. 《Plant foods for human nutrition (Dordrecht, Netherlands)》2001,56(4):349-358

The effect of dietary hesperetin on the hepatic lipid content and the enzyme activities involved in triacylglycerol (TG) synthesis in rats fed diets with or without 1% orotic acid (OA) was studied. Hepatic TG content was raised by approximately 5-fold after administration of OA for 10 days. The OA-feeding significantly increased the activity of hepatic microsomal phosphatidate phosphohydrolase (PAP), which is the rate-limiting enzyme for TG synthesis. Hepatic glucose-6-phosphate dehydrogenase (G6PDH) and malic enzyme activities were also increased. An addition of 1% hesperetin to the OA-supplemented diet resulted in the decrease of the hepatic TG content by 44% and of microsomal PAP activity. Dietary hesperetin alone neither affected liver TG content nor PAP activity significantly. OA-feeding caused an increased liver cholesterol level, whereas simultaneous addition of hesperetin and OA reduced its content to the control level. A slight reduction of hepatic cholesterol by hesperetin was also observed in the OA-free dietary group. The present study demonstrated that dietary hesperetin can reduce the hepatic TG accumulation induced by OA, and this was associated with the reduced activity of TG synthetic enzyme, PAP.  相似文献   

豆粕基础饲料中添加胆固醇促进鱼类生长机理的研究进展     

龙晓文  王秋举  汉雪梅  张曦  邓君明 《安徽农业科学》2013,(7):2954-2955

胆固醇是1种环戊烷多氢菲的衍生物,是动物组织细胞所不可缺少的重要物质。在豆粕替代鱼粉的饲料中添加适量胆固醇对鱼类有一定的促生长作用。目前国内外对胆固醇促鱼类生长机理的研究还处于初步探讨阶段。综述了近年来豆粕基础饲料中添加胆固醇对鱼类摄食、生长、消化酶、免疫力、胆固醇代谢及体组成等方面的影响。对胆固醇促进鱼类生长的机理进行了探讨,旨在为胆固醇促鱼类生长机理的研究提供一定思路和线索。  相似文献   

The Effect of Cholesterol Addition,Buffer, and pH on Equine Sperm Stored at 5°C     

André M. Crespilho  Beth E. Spizziri  Mindy Meyers  James K. Graham 《Journal of Equine Veterinary Science》2013

Cooled stallion semen has a short viable life, which ranges with acceptable motility and viability from 24 up to 48 hours. The purpose of this study was to compare the effects of storage pH, the ability of three different zwitterionic buffers, and cholesterol-loaded cyclodextrins (CLC) to preserve the motility and integrity of stallion sperm cooled to 5°C for 48 hours. Fourteen ejaculates were collected and split to receive CLC or not (control group). After incubation, each sample was split into six subsamples and diluted in KMT extender containing 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES), or 2-(N-morpholino)ethanesulfonic acid (MES) buffers, and the final pH was adjusted to either 7.0 or 6.6, totalizing 12 experimental groups as a function of CLC, buffer, and pH variables (2 × 3 × 2 factorial). The motility parameters and integrity of plasma and acrosome membranes (live cell index) were determined using computer-automated semen analysis and epifluorescence microscopy at 3, 6, 24, and 48 hours of cooling period. According to results, pH was not a significant source of variation for motility and live sperm over different cooling periods. However, samples diluted in BES exhibited higher progressive motility within 3 hours and higher percentages of total motile cells after 48 hours of incubation at 5°C (P < .05). After 24 hours of storage, CLC-treated sperm samples presented higher motility than control group (P < .05), and after 48 hours of incubation, CLC-treated sperm exhibited higher percentages of live, motile, and progressively motile sperms (P < .05). We inferred that equine semen diluted in KMT containing BES as buffer and CLC treatment improve the equine sperm survival during storage at 5°C for 48 hours.  相似文献   

鸡 HMGCR 基因SNPs及其与鸡蛋胆固醇含量的关联性分析     

魏杨  徐亚铂  刘向波  康相涛  田亚东 《家畜生态学报》2013,34(3):16-20

为培育一种蛋黄胆固醇含量较低的蛋鸡新品系,以丝羽乌骨鸡为研究对象,测定675枚丝羽乌骨鸡蛋蛋黄胆固醇含量,通过DNA测序技术和创造酶切位点PCR-RFLP方法检测了丝羽乌骨鸡群体中3-羟基-3-甲基戊二酸单酰辅酶A还原酶基因的2个SNPs位点,分别分析这两个SNPs位点不同基因型与鸡蛋胆固醇含量的关联性。结果表明:(1)丝羽乌骨鸡蛋蛋黄胆固醇含量为13.41mg/g,鸡蛋中胆固醇含量为456.50mg/100g。(2)g.12217G>T位点中GG基因型的鸡蛋胆固醇含量最低,且与其他两种基因型具有显著的差异。  相似文献   

虾Y-器官7、8-脱氢酶转化胆固醇为7-脱氢胆固醇研究     

黄时海  何鑫平  于配配  曹喜秀  曹普美  吴孔阳  李湘萍 《安徽农业科学》2011,39(25):15397-15399

[目的]以虾Y-器官微粒体7、8-脱氢酶催化胆固醇,制备7-脱氢胆固醇。[方法]通过单因素试验,研究影响转化反应的各种因素。[结果]将摘除眼柄的刀额新对虾（Metapenaeus ensis）Y-器官,匀浆、离心制备的微粒体酶液,于22℃与胆固醇避光反应6 h,可以积累相对较多的7-脱氢胆固醇。[结论]该研究为利用虾Y-器官7、8-脱氢酶转化胆固醇为7-脱氢胆固醇奠定基础。  相似文献