Biochemical response in rats to the addition of curry leaf (Murraya koenigii) and mustard seeds (Brassica juncea) to the diet     

Beena A. Khan  Annie Abraham  S. Leelamma 《Plant foods for human nutrition (Dordrecht, Netherlands)》1996,49(4):295-299

Three groups with 12 weanling male albino rats were group fed for 90 days on a standard laboratory rat diet plus 20% coconut oil either without a supplement or with the addition of 10% curry leaf or 10% mustard seeds. Feed was offered at a level of 10% body weight. At the end of the trial, measurements were made on the total serum cholesterol, high density lipoproteins, low density lipoproteins, and very low density lipoproteins fractions, release of lipoproteins into the circulation, lecithin cholesterol acyl transferase activity and lipoprotein lipase activity. Feed intake and mean body weight of the rats on each treatment was not significantly different. Both spices resulted in a reduction in total serum cholesterol and LDL+VLDL, an increase in the HDL, lower release of lipoproteins into the circulation and an increase in the LCAT activity.  相似文献   

Cholesterol depletion up-regulates expression of prohibitin in human prostate carcinoma PC-3 cells     

DONG Pei  LI Yong-hong  YAO Kai  LIU Zhuo-wei  ZHOU Fang-jian 《园艺学报》2011,27(11):2116-2119

AIM: To study how cholesterol delpetion affects prohibitin expression and the development of prostate cancer. METHODS: Human prostate carcinoma PC-3 cells were cultured in normal medium (NM) and cholesterol delpetion medium (CDM) for 48 h.The mRNA expression of prohibitin was detected by real-time PCR. Prohibitin promoter was cloned and inserted into PGL3-Basic to reconstruct plasmid. The plasmid was transiently transfected into PC-3 cells. The cells were cultured in the medium with different concentrations of cholesterol for 48 h and luciferase expression was detected.RESULTS: The results of real-time PCR showed that the mRNA level of prohibitin increased about 19 times in PC-3 cells in the presence of CDM. After transfected with prohibitin promoter plasmid for 48 h, PC-3 cells cultured in CDM had higher luciferase expression than the cells cultured in NM or in CDM with cholesterol. CONCLUSION: Cholesterol depletion up-regulates prohibitin expression in PC-3 cells, which may be one of the self-prophylactic and regulatory mechanisms to protect PC-3 cells from apoptosis caused by cholesterol insufficiency.  相似文献   

桑叶粉对鸡蛋蛋黄胆固醇含量和脂肪酸组成的影响   总被引：2，自引：0，他引：2  

王道营  卞欢  诸永志  刘芳  徐为民  邹川洪 《江西农业学报》2011,23(8):139-140,143

分析了在饲料中添加不同量的桑叶粉对鸡蛋蛋黄胆固醇含量和脂肪酸组成的影响,结果表明:不同桑叶添加量对鸡蛋蛋黄胆固醇含量及脂肪酸组成具有显著影响,当饲料中添加7%桑叶时,与未添加桑叶组相比,其蛋黄胆固醇含量下降9.24%,多不饱和脂肪酸比例增加23.63%,因此桑叶对提升鸡蛋品质具有显著的作用。  相似文献   

Effect of ginkgolide B on cholesterol content in PC12 cells under high cholesterol culture     

GUO Mao-juan  FAN Ying-chang  LU Bin  JIANG Xi-juan 《园艺学报》2012,(1):173-176

AIM: To explore the effect of ginkgolide B (GB) on high cholesterol-induced dysfunction in PC12 cells. METHODS: The rat brain microvascular endothelial cells and astrocytes were used to establish the blood-brain barrier (BBB) model by in vitro co-culture in the Transwell device with PC12 cells. Cholesterol at the concentration of 40 μmol/L was added into the side of brain microvascular endothelial cells of the Transwell. Various concentrations of GB were applied to deal with the culture system. The morphological changes of the cells were observed under microscope. The safe doses of GB were screened by MTT assay. The level of cholesterol in the PC12 cells was determined by the high-performance liquid chromatography. RESULTS: The morphology of PC12 cells in control group was normal,with round,short spindle or triangle shape,cytoplasm extension,two-pole short processes,good refraction and obvious nucleus.The doses of GB determined by MTT assay were 1 μmol/L for low dose,5 μmol/L for middle dose and 25 μmol/L for high dose. Compared with control group, the cholesterol obviously increased in model group with statistical difference (P<0.05). GB at dose of 25 μmol/L for 16 h exposure had the optimum results to decrease the cholesterol level in PC12 cells. However, no significant difference between GB high dose group and simvastatin group was observed (P>0.05). CONCLUSION: The active monomer of ginkgo leaf, GB, may effectively reduce the cholesterol content in PC12 cells with the condition of BBB in vitro.  相似文献   

ox-LDL increases endothelial lipase in RAW264.7 macrophages     

WU Xiao-qian  YI Quan  HE Li-shan 《园艺学报》2012,28(7):1172-1175

AIM: To investigate the effect of oxidized low-density lipoprotein (ox-LDL) on the expression of endothelial lipase (EL) in murine RAW264.7 macrophages. METHODS: RAW264.7 cells were incubated with ox-LDL at concentrations of 0~100 mg/L for 24 h.On the other hand, the cells were incubated with or without PDTC (NF-κB inhibitor) for 30 min and then with ox-LDL (50 mg/L) for 24 h. The expression of EL and p65 was detected by Western blotting. RESULTS: The level of EL was significantly increased after ox-LDL incubation in RAW264.7 cells (P<0.05). NF-κB was activated by ox-LDL at concentration of 50 mg/L for 15~30 min in RAW264.7 cells. The increase in EL induced by ox-LDL was markedly inhibited by a NF-κB inhibitor PDTC (P<0.05). CONCLUSION: ox-LDL significantly increases the expression of EL in RAW264.7 macrophages, which is possibly related to NF-κB activation.  相似文献   

胆固醇对鸡传染性支气管炎病毒感染鸡胚肾细胞的影响     

李广兴  赵蕾  陈慧杰  郎跃深  刘鹏  黄小丹  王洪微  任玉东  张瑞莉 《东北农业大学学报》2017,48(4)

胆固醇是维持细胞膜脂筏稳定结构重要组分。一些囊膜病毒感染过程依赖胆固醇。研究利用Real Time PCR和病毒滴度试验,分析细胞膜和病毒囊膜胆固醇是否影响鸡传染性支气管炎病毒(IBV)感染鸡胚肾(CEK)细胞。结果表明,胆固醇萃取剂甲基-β-环糊精去除细胞膜胆固醇后,可抑制IBV感染CEK细胞并呈剂量依赖性,补充外源性胆固醇后,IBV感染力部分恢复,说明IBV感染依赖细胞膜胆固醇;去除细胞膜胆固醇可影响IBV感染侵入和释放过程。去除病毒囊膜胆固醇对IBV感染力无显著影响。IBV感染CEK细胞时依赖细胞膜胆固醇,而非病毒囊膜胆固醇。  相似文献   

Clinical Relationship between Cholestatic Disease and Pituitary‐Dependent Hyperadrenocorticism in Dogs: A Retrospective Case Series          下载免费PDF全文

K.‐h. Kim  S.‐m. Han  K.‐o. Jeon  H.‐t. Kim  Q. Li  M.‐o. Ryu  W.‐j. Song  S.‐c. Park  H.‐y. Youn 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2017,31(2):335-342

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高产胆固醇氧化酶菌株的诱变选育     

周小楠  董群 《安徽农业科学》2009,37(4):1576-1577

[目的]选育高产胆固醇氧化酶菌株。[方法]从环链拟青霉、斜链拟青霉、中国拟青霉和蛹拟青霉中筛选出发菌株,对筛选出的酶活力高的突变株进行紫外诱变,选育出高产胆固醇氧化酶突变株,对其发酵条件进行优化并测定其酶活。[结果]选择环链拟青霉为诱变选育的出发菌株,紫外诱变环链拟青霉获得了一株高产胆固醇氧化酶突变株CM3,确定了紫外诱变的最佳时间是80S。通过优化其发酵条件,得出CM3发酵产酶最优条件为30℃,pH值6．0,接种量为10％。其最高酶活力为1．3360U／ml,比出发菌株（0．3369U／m1）提高了296．6％。[结论]通过紫外诱变能选育出有较高胆固醇氧化酶活力的菌株。  相似文献   

Effects of ATP binding cassette transporter A1 on cholesterol efflux in macrophages     

TANG Chao-ke  YAN Peng-ke  YANG Yong-zong 《园艺学报》2003,19(10):1427-1431

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Mast cells change cellular cholesterol content and inhibit cholesterol efflux from THP-1 macrophage-derived foam cells     

ZHAO Zhan-zhi  YANG Yong-zong  WANG Zuo  WEI Dang-heng 《园艺学报》2007,23(8):1457-1463

AIM:Mast cells (MC) are present in the arterial intima,the site of atherogenesis. The present studies explore the effect of MC on cholesterol content,distribution and efflux in THP-1 macrophage-derived foam cells (THP-1FCs）. METHODS:THP-1FCs were incubated with high-density lipoproteins 3 (HDL₃) in the absence or presence of mast cell granules (MCGs) harvested from compound 48/80-stimulated rat peritoneal MC. The intracellular cholesterol level,cholesterol effluxing capacity,ATP-binding cassette transporter A1(ABCA1) mRNA and HDL₃ treated with MCGs were detected to characterize the role of MC on intracellular cholesterol. RESULTS:MCGs had high levels of cellular total cholesterol(TC),free cholesterol(FC) but not esterifed cholesterol(EC) compared to control group where the TC concentrations ranged from 527.3 mg/g to 917.9 mg/g cellular protein with EC accounting for 7.6% of the cholesterol. Cholesterol efflux was 14% less in MCGs group compared to control group. ABCA1 mRNA expression in MCG-treated THP-1FCs remained unchanged in 20 hours. In contrast,treatment of HDL₃ with MCGs resulted in rapid degradation of the main HDL₃ apoliproteins,apoA-Ⅰ. SDS-PAGE revealed that a minor polypeptide band with about 26 kD molecular mass appeared below the apoA-Ⅰband. Densitometric analysis of the gel demonstrated that ≈ 28% of apoA-Ⅰhad been degraded by the MCGs. CONCLUSION:These results indicate that MC decreases cholesterol efflux,increases cellular accumulation in TC and FC by depleting HDL₃ and apoA-Ⅰ,but not by inhibiting ABCA1 mRNA expression.  相似文献