青花菜肉桂酰辅酶A还原酶基因的克隆与表达特征分析     

李小艳  裴徐梨  荆赞革  唐征 《热带作物学报》2016,37(11):2199-2203

为研究肉桂酰辅酶A还原酶在青花菜生长发育中的作用,采用RT-PCR技术克隆青花菜Bo CCR基因全长c DNA序列,并利用q RT-PCR检测其在不同器官中的表达模式。结果表明:Bo CCR基因开放阅读框为987 bp,推导编码328个氨基酸。预测蛋白分子量为36.86 ku,PI值为6.04。该蛋白亲水性氨基酸多于疏水性氨基酸,为亲水性蛋白。高级结构预测表明青花菜Bo CCR蛋白具有完整的二级结构,三级结构的α-螺旋主要位于外部,β-折叠位于内部。分子进化分析显示CCR蛋白在植物进化过程中,各属形成单独的分枝,可以区分其亲缘关系。荧光定量技术检测到青花菜的Bo CCR基因在花蕾发育早期表达最高,推测该基因可能与青花菜花粉发育相关。  相似文献   

Reproductive barriers to hybridizations between narrow-leaf and broad-leaf Anoectochilus roxburghii     

Renda Teng  Mei Wu  Hongzhen Wang  Mingyan Li  Yuqiu Huang 《The Journal of Horticultural Science and Biotechnology》2017,92(2):183-191

Narrow-leaf and broad-leaf Anoectochilus roxburghii were used as reciprocal parents to explore the reason for low seed setting rate. Pollen-pistil interaction was observed by fluorescence microscopy and hybrid embryo development by paraffin section technology. The results indicated that pollen tube growth could reach the embryo sac, and double fertilization could be completed. These findings showed that a pre-fertilization barrier was not the major factor in the cross of narrow-leaf and broad-leaf A. roxburghii. The endosperm development of the hybrid was abnormal and eventually promoted abortion of the embryos. A post-fertilization barrier appears to be the major factor for low seed setting rate in the cross of narrow-leaf and broad-leaf A. roxburghii. In addition, an efficient protocol of embryo rescue, the development of which is reported elsewhere, was used to compare the time needed for rescue between the reciprocal crosses.  相似文献   

Drag force and reconfiguration of cultivated Saccharina latissima in current     

《Aquacultural Engineering》2021

The design of aquaculture systems requires an understanding of the drag forces on cultivated kelp. This study measured the drag on line segments of cultivated Saccharina latissima in a towing tank. The drag on segments of farm line with full kelp bundles and with stipes alone (fronds removed) was measured at tow speeds of 0.10 to 0.50 m/s. The drag on individual fronds cut from the line was also measured. Video images were collected to evaluate the plant reconfiguration. Both kelp blades and stipes contributed to the total drag force on the line bundle. Within the velocity range of our experiments, the kelp blades were essentially horizontal. However, the pronation of kelp stipes increased as flow velocity increased. The reconfiguration of kelp stipes was observed to decrease the vertical extent of the kelp bundle. Due to this reconfiguration, the measured force, $F$, increased with velocity, $U$, at a rate slower than quadratic, and was consistent with scaling laws derived for reconfiguration. Specifically, $F\sim U^{\alpha }$ with $\alpha =1.35\pm 0.17$.  相似文献   

Identification of the core motif of the BRCA2 C-terminal RAD51-binding domain by comparing canine and human BRCA2     

Yasunaga YOSHIKAWA  Masami MORIMATSU  Kazuhiko OCHIAI  Toshina ISHIGURO-OONUMA  Ryo MORIOKA  Kento OKUDA  Koichi ORINO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):759

Mammary tumors are the most common tumors in women and non-spayed female dogs. One of the reasons for mammary tumors is mutations of the tumor suppressor gene, BRCA2. BRCA2 participates in homologous recombination repair by interacting with the RAD51 recombinase. BRCA2 has two RAD51-binding domains, consisting of BRC repeats and the C-terminal RAD51-binding domain, respectively. Although several studies have addressed the function of the C-terminal RAD51-binding domain of human BRCA2, the amino acid sequences required for the RAD51-interaction activity remain unclear. In this study, the C-terminal RAD51-binding domains of canine and human BRCA2 were compared; the canine domain displayed a weaker interaction with RAD51. This difference was attributed to the C-terminal portion of the domain via a comparison between canine and human domains. Furthermore, peptides shorter than those previously reported displayed RAD51-interacting activity, and a core motif of this domain consisting of 25 amino acids was identified. Since a mutation (S3323N) was reported in the core motif of this domain, the effect of this mutation was evaluated. The mutant exhibited similar RAD51-binding activity as that of the wild-type protein, suggesting that the mutation was functionally neutral. These data suggested that the C-terminal portion of the BRCA2 C-terminal RAD51-binding domain influenced its RAD51-interaction activity, and a minimum core motif of 25 amino acids was identified in this domain. These data may help clarify BRCA2 function, as well as the tumorigenic effects of BRCA2 mutation.  相似文献   

Roles of cell differentiation factors in preimplantation development of domestic animals     

Ken SAWAI 《The Journal of reproduction and development》2021,67(3):161

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Predicting Acceptability of Jaguars and Pumas in the Atlantic Forest,Brazil     

Monica T. Engel  Jerry J. Vaske  Alistair J. Bath  Silvio Marchini 《Human Dimensions of Wildlife》2016,21(5):427-444

Jaguars and pumas are threatened species in Brazil’s Atlantic Forest, especially at the borders of protected areas. This article assessed the influence of emotions, attitudes, existence value, and agency credibility on acceptability of big cats among rural residents living adjacent to two protected areas in this forest. Data from self-administrated questionnaires (n = 326) indicated those with positive attitudes toward big cats (β = .28, p < .001), those who valued the existence of big cats (β = .14, p < .05), those who would feel sorrow if big cats disappeared (β = .21, p < .001), and those who considered the managing agency as credible (β = .16, p = .002) were more accepting of big cats. The model provided theoretical and practical insights into large carnivore conservation. For example, given the significance of agency credibility, a positive relationship between park authorities and residents is crucial for big cat conservation.  相似文献   

Molecular cloning and tissue distribution of a short form chicken leptin receptor mRNA     

Liu X  Dunn IC  Sharp PJ  Boswell T 《Domestic animal endocrinology》2007,32(3):155-166

In mammals, alternative splicing of the leptin receptor (LEPR) produces several C-terminal truncated isoforms that are believed to play a role in the transport, cellular internalisation and degradation of the hormone leptin. The chicken leptin receptor (chLEPR) is similar to its mammalian counterparts in terms of its intron/exon structure and conserved motifs. However, it is unknown whether the chLEPR also undergoes alternative splicing. To test this, structural analysis of intron 19 of the chLEPR, equivalent to the intron in which alternative splicing occurs in mammals, was combined with 3'-rapid amplification of cDNA ends (3'-RACE) to search for chLEPR splice variants. A 44-amino acid alternative exon 20 was identified that is spliced to generate a short isoform of the chLEPR (chLEPR-SF). Comparative sequence analysis of intron 19 identified two regions that are highly conserved between the chicken and mammals, indicating their possible importance as intronic elements in the regulation of alternative splicing of the LEPR in vertebrates. Tissue expression of the chLEPR-SF was lower and more restricted than that of the chLEPR long isoform. Collectively these data demonstrate that the chLEPR is alternatively spliced to produce at least one short isoform, as is the case in mammals.  相似文献   

短花针茅荒漠草原物种间关系的多度不对称性对载畜率的响应     

张彬  张峰  郑佳华  王琪  李雅男  乔荠瑢  赵天启  于跃  赵萌莉 《草地学报》2023,31(2):510-517

目前物种的相对多度与种间关系的内部联系如何影响物种共存仍不清楚。为探讨植物群落物种间关系强度的多度不对称性及其对载畜率的响应,本研究以内蒙古短花针茅(Stipa breviflora)荒漠草原植物群落为对象,分析了对照、轻度、中度及重度放牧下群落物种的多度及其频率。结果表明：随载畜率的增大,物种数呈现出降低的趋势。不同载畜率下同分布指数CO均存在明显的多度依赖性;常见种(如短花针茅、无芒隐子草(Cleistogenes songorica)及刺穗藜(Chenopodum aristatum))对其他物种的作用强度普遍较强。不同载畜率下短花针茅对无芒隐子草及刺穗藜的作用强度均高于其二者对短花针茅的强度,表现出种间强度的不对称性。此外,放牧使得短花针茅对无芒隐子草的作用强度呈现出增大的趋势,使得无芒隐子草对短花针茅的作用强度呈现出降低的趋势。因此,物种间存在多度的不对称性,而放牧进一步造成其物种间作用强度的变化。  相似文献   

分子标记技术及其在稻瘟病抗性基因中的应用     

王羡国  何琳  靳学慧  张亚玲 《种子世界》2006,(2):32-34

分子标记技术是近20年发展起来的一种分子生物学技术，具有准确度高、多态性高、表现共显性等特点。根据检测手段的不同，分子标记技术可分为以DNA-DNA杂交为基础、以PCR技术为基础、以DNA杂交和PCR技术相结合为基础和以单核苷酸多态性为基础的4种类型分子标记技术。研究者利用各种分子标记技术已鉴定出30多个水稻抗稻瘟病基因，并进行了基因定位，其中抗稻瘟病基因Pi-ta和Pi-b已被克隆。本文简要介绍了多种分子标记技术及其在抗稻瘟病基因研究中的应用进展。  相似文献   

新城疫病毒La Sota株HN基因的克隆与序列分析     

刘颖  金丽颖  申燕  高冬妮  平文祥  葛菁萍 《中国农学通报》2015,31(14):89-95

旨在从分子生物学角度进一步研究新城疫病毒La Sota株HN基因,探究NDV La Sota HN基因的遗传变异情况。研究以试验室冻存的p NDV-HN为模版,根据Gen Bank中登录的NDV La Sota株序列设计引物扩增HN基因,将其克隆到pMD18-T载体后进行鉴定,对鉴定正确的重组质粒p MD-NDV-HN进行测序分析。应用生物信息学软件对新城疫病毒Lasota株HN基因进行系统进化树分析、氨基酸序列同源性分析、糖基化位点、蛋白结构跨膜区分析及磷酸化位点预测分析。核苷酸序列测定结果表明:HN基因的序列长度为1743bp,该基因的开放阅读框(Open Reading Frame,ORF)总长为1716 bp,编码571个氨基酸。生物信息学表明:试验获得的HN基因具有6个潜在糖基化位点及12个半胱氨酸残基,第5个潜在糖基化位点(508-510 aa)发生缺失及第123位半胱氨酸残基被色氨酸残基取代。La Sota株HN基因编码的蛋白质中有29个丝氨酸、10个酪氨酸和16个苏氨酸可能成为蛋白激酶磷酸化位点。将试验获得的La Sota株HN基因序列和推导的氨基酸序列与新城疫毒株的HN基因相应序列比较后发现,它们的核苷酸序列同源性和氨基酸序列同源性最高均可达到99%,表明HN基因在种属间具有较高的保守性。研究为新城疫病毒的分子病毒学研究奠定了基础。  相似文献