Nitrogen isotope analysis of free glycine in soil using isotope dilution mass spectrometry     

Elin Norlin  Knut Irgum 《Soil biology & biochemistry》2007,39(2):550-559

To enable the estimation of production and consumption rates of free glycine in soils through ¹⁵N isotope dilution experiments, an isotope dilution mass spectrometric method was developed. The method, which enabled high precision N isotope ratio determination of glycine in soil extracts at δ¹⁵N levels up to 4000‰ and concentrations from approximately 2 μM, is based on the following steps: (i) addition of glycine spike to the soil extract, (ii) removal of humic substances and pre-concentration of glycine using solid phase extraction, (iii) derivatization of amino acids, (iv) separation of the derivatives using gas chromatography (GC), (v) their combustion to yield sample N₂ gas, and (vi) finally the use of N isotope ratio mass spectrometry (IRMS). Judging by uncertainty budget calculations, the precision obtained (SD=0.01-0.06 at% ¹⁵N) is sufficient for detecting differences in N isotopic ratios obtained in ¹⁵N isotope dilution experiments.  相似文献   

Identification of quantitative trait loci for partial resistance to Phytophthora sojae in soybean     

J. Zhao  N. Guo  B. Zhang  F. Yang  S. Chen  J. Gai  H. Xing 《Plant Breeding》2011,130(2):144-149

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Sampling Survey and Identification of Races of Soybean Cyst Nematode (Heterodera glycines Ichinohe) in Huang-Huai Valleys     

LU Wei-guo  GAI Jun-yi  LI Wei-dong 《中国农业科学(英文版)》2006,5(8)

Soybean cyst nematode (SCN Heterodera glycines Ichinohe) is one of the most important nationwide soybean diseases in China. A total of 38 soil specimens or locations in the area was sampled and tested for SCN races during 2001-2003 for the inspection of race distribution in Huang-Huai Valleys. A map of race distribution was constructed according to the data from both the present study and the published reports cited. Three areas, namely, the area of southeast to Jinan in Shangdong Province; the area of northern Henan Province and its border region to south of Hebei Province; and the area of Luohe, Zhoukou of Henan Province and Fuyang of Anhui Province mainly infested with Race 1 were identified. Race 4 was predominant in Shanxi Province, Beijing and the adjacent area of Henan, Shandong, and Anhui provinces, and the delta of Huanghe River in Shandong Province. Race 2 was mainly found in Liaocheng, Dezhou of Shangdong Province and Shijiazhuang of Hebei Province, and Jiaozuo and Huojia of Henan Province. Race 7 was distributed in the west part of Jiaodong Peninsula of Shandong Province and Kaifeng, Huaxian, Wenxian of Henan Province. Race 5 was found and scattered in Hebei and Henan Province. Race 9 was found in Shangqiu of Henan Province, which was reported for the first time in China. It can be seen that Race 1 and Race 4 were the two predominant races in Huang-Huai Valleys, and that research should focus on developing resistant cultivars of these races. There might exist other races in an area with some predominant races. The race substitution in the past decade was not obviously found, therefore, the results should be meaningful to future breeding for resistance to SCN in Huang-Huai Valleys.  相似文献   

不同结荚习性大豆品种综合生产力的分析评价   总被引：2，自引：0，他引：2  

温学发  王海英  张惠君  杨艳琴  谢甫绨 《沈阳农业大学学报》2005,36(2):143-147

采用灰色关联度分析法对不同结荚习性大豆的综合生产力进行了两年的综合评价研究。结果表明，在一般肥力条件下，亚有限型结荚习性大豆综合生产力优于有限型结荚习性大豆。亚有限型大豆辽豆14号的产量及综合生产力都显著优于其他品种（系）。而在育种目标相似的情况下，不同结荚习性大豆间产量差异不显著。对不同结荚习性大豆来说，产量构成主导因素均是单株荚数、单株分枝荚数。努力提高大豆单株荚数是今后大豆育种的主攻方向。  相似文献   

大豆结实前异黄酮积累规律的研究   总被引：4，自引：0，他引：4  

董李平  吴珍龄  易泽林 《西南大学学报(自然科学版)》2004,26(6):756-758

选取异黄酮含量显著不同的4个品种为实验材料,研究了大豆结实前异黄酮在组织中的分布、变化及其积累规律。结果表明,大豆结实前种子中贮存的异黄酮逐步由种子向子叶,继而向其他器官(主要是叶片)转移,不同器官在植株生长发育过程中进行着异黄酮类物质的代谢。该时期异黄酮的分布存在组织特异性而品种间的差异不明显,各组织问的差异达到极显著水平：在大豆出苗期,地上部分的异黄酮含量远高于地下部分;幼苗期子叶中异黄酮远高于其它部分;壮苗期和盛花期,叶片中异黄酮的含量远高于其他组织。  相似文献   

大豆对SMV数量抗性的表现形式与种质鉴定   总被引：7，自引：1，他引：7  

智海剑  盖钧镒 《中国农业科学》2004,37(10):1422-1427

 在发现大豆对SMV具有数量抗性的基础上，选用96份大豆品种材料研究其在接种Sa、SC8、N1、N3等4个SMV株系条件下的发病率、病级、潜育期和病害扩展速度等4个数量抗性组分的变异。结果表明，品种间4个组分均存在明显差异；溧水中子黄豆、沛县天鹅蛋、诱变30等品种虽然对4个株系均感染，但在4个组分上均表现出较强抗性，且不同株系间差异较小，说明这些品种对大豆花叶病毒具有广谱数量抗性。研究证实以往报道的一些抗感染品种如溧水中子黄豆、AGS-19,其实是抗扩展的数量抗性品种。邳县茶豆、淮阴秋黑豆等品种对SMV的抗性可能属于数量抗性与质量抗性的叠和。大豆对SMV的数量抗性是曾被学术界忽视而又值得利用的一类抗性，它一般比质量抗性具有更宽的抗谱和更好的持久性。  相似文献   

利用MPCR技术快速检测进口大豆转基因背景   总被引：4，自引：0，他引：4  

徐景升  余爱丽  姚伟  饶进  张木清  陈如凯 《江西农业大学学报》2005,27(2):262-264,269

利用改良的CATB方法提取大豆(Glycine max)种子基因组DNA。以大豆内源ScII基因做对照，采用MPCR(Multiplex Polymerase Chain Reaction，MPCR)技术从进口大豆中检测出35S启动子、NOS终止子和EPSPS耐除草剂基因3种转基因成分。  相似文献   

钙素和水分亏缺对大豆幼苗某些生理过程的影响   总被引：7，自引：0，他引：7  

杨根平  盛宏达  赵彩霞 《西北农林科技大学学报(自然科学版)》1990,(2):84-87

在水分胁迫下大豆幼苗叶片相对水分含量(RWC)和光合速率下降,细胞质膜透性、脯氨酸(Pro)含量和气孔阻力均增大;缺钙与供钙植株相比,其RWC和光合速率更低,膜透性改变剧烈,特别是Pro增加2～3倍,缺钙处理下光合速率的进一步降低,可能由非气孔因素所致。实验结果表明钙对改善植物的水分状况、提高膜的稳定性和植株抗旱性具有重要作用。  相似文献   

三种黑色粮油作物种皮花色苷提取物抗氧化能力的稳定性比较   总被引：7，自引：0，他引：7  

李莉蓉  张名位  刘邻渭  魏振承  张瑞芬 《中国农业科学》2007,40(9):2045-2052

 【目的】比较外界因素及杀菌工艺对黑米、黑大豆、黑玉米3种黑色粮油作物种皮花色苷提取物总抗氧化能力的影响。【方法】选用不同光照、温度、食品原料、防腐剂、金属离子和5种杀菌工艺（巴氏灭菌、煮沸灭菌、高温短时灭菌、高压蒸汽灭菌、微波灭菌、）处理3种花色苷提取物，采用铁离子还原法评价其处理前后的总抗氧化能力变化。【结果】黑米、黑大豆、黑玉米种皮花色苷提取物的总抗氧化能力随光照和加热时间延长而下降，温度越高下降越快，在避光、自然光和日光灯照射3种光照条件下黑米花色苷提取物的稳定性最好，其次为黑大豆花色苷提取物的，黑玉米花色苷提取物的最差；在相同温度下黑大豆花色苷提取物的稳定性最好，黑米和黑玉米花色苷提取物的较差；NaCl、蔗糖、葡萄糖等食品原料和防腐剂苯甲酸钠对3种花色苷提取物总抗氧化能力的影响不明显，5种金属离子（K+、Ca2+、Mg2+、Zn2+、Cu2+）和5种不同杀菌工艺（巴氏灭菌、煮沸灭菌、高温短时灭菌、高压蒸汽灭菌、微波灭菌）对其总抗氧化能力均有不同程度的影响，其中高温短时灭菌的影响较小，高压蒸汽灭菌的影响最大。【结论】黑米、黑大豆、黑玉米3种花色苷提取物抗氧化稳定性受光照、温度、食品原料、金属离子和杀菌工艺等多种因素影响。  相似文献   

水分胁迫下水杨酸对毛豆幼苗生长及其抗渍性的影响     

吴建国  陆晓民  张晓婷  陈林  李志国 《勤云标准版测试》2006,(1)

在水分胁迫下采用不同浓度的水杨酸对毛豆幼苗进行叶面喷施处理,测定其对毛豆幼苗生长及其抗渍性的影响。试验结果表明:采用一定浓度的水杨酸处理可以促进苗生长、提高根系活力、叶绿素含量、脯氨酸含量和抑制丙二醛增生、降低细胞膜透性,增强了毛豆幼苗对渍水环境的抵抗能力。从叶面喷施对毛豆的营养指标和生理指标进行综合判断可知:在试验的范围内,以2mmol/L水杨酸的处理效果为最佳  相似文献