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61.
AIM:To investigate whether the bcl-2 antisense oligonucleotide increases the sensitivity of HL60 and K562 cell lines to daunorubicin.METHODS:IC50 for HL60 and K562 was determined with MTT method, the expression levels of Bcl-2 protein were assayed by immunofluorescence using fluoresce isothiocyanate labeling. In addition, apoptosis was detected by morphological observation and flow cytometric analysis of DNA fragmentation.RESULTS:It was found that the two oligonucleotides directed against the coding region and the translation initiation of bcl-2 mRNA, combined respectively with daunorubicin, inhibited expression of bcl-2 protein, increased apoptosis in HL60 and K562 cells, and decreased IC50 of daunorubicin significantly (P<0.05). Compared to the antisense oligonucleotide directed against the translation initiation of bcl-2 mRNA, the antisense oligonucleotide directed against the coding region showed stronger effects in the aspects of increasing the sensitivity of HL60 cells to daunorubicin (P<0.05).CONCLUSIONS:These two antisense sequences in the translation initiation and the coding region of bcl-2 mRNA increased the sensitivity of HL60 and K562 cell lines to daunorubicin in a sequence-specific manner. 相似文献
62.
Summary Stem thickness of the weed Solanum nigrum and the crop sugarbeet was determined with a He–Ne laser using a novel non‐destructive technique measuring stem shadow. Thereafter, the stems were cut close to the soil surface with a CO2 laser. Treatments were carried out on pot plants, grown in the greenhouse, at two different growth stages, and plant dry matter was measured 2–5 weeks after treatment. The relationship between plant dry weight and laser energy was analysed using two different non‐linear dose–response regression models; one model included stem thickness as a variable, the other did not. A binary model was also tested. The non‐linear model incorporating stem thickness described the data best, indicating that it would be possible to optimize laser cutting by measuring stem thickness before cutting. The general tendency was that more energy was needed the thicker the stem. Energy uses on a field scale are discussed. 相似文献
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64.
几种落叶果树H2O2含量变化与自然休眠关系的研究 总被引:18,自引:2,他引:18
以设施栽培中常见的几种核果类果树品种和两个葡萄品种为试材, 分析了芽休眠期间 H2O2含量变化动态, 并探讨了温度、生长调节剂及化学破眠物质对H2O2含量影响的效应。结果表明: 休眠期间,不同树种( 品种) 芽内 H2O2含量存在差异, 基本趋势是晚熟品种高于早熟品种, 花芽高于叶芽, 但葡萄品种相反, 早熟的‘京秀’高于晚熟的‘巨峰’; 休眠期芽内 H2O2含量基本呈稳步上升后急剧下降的趋势,不同品种急剧下降的时间略有差别, 且与自然休眠解除的时间相吻合。低温(5 ℃) 处理显著增加了芽中 H2O2含量, 中温(10 ℃) 使 H2O2含量略有增加, 而高温(20 ℃) 却导致 H2O2含量降低。休眠前期50 mg.L-1 ABA 处理显著提高了芽中H2O2含量, 而100 mg.L-1的GA3和6-BA 处理有减少 H2O2含量的趋势, 但二者差异不明显。热带地区常用的化学破眠物质对芽 H2O2的影响因树种( 品种) 、使用时期不同而异, 硫脲、KNO3前期使用对核果类果树影响明显, CaCN2对核果类无明显效应, 但对葡萄品种作用显著。果树芽 H2O2含量的动态变化表明, H2O2可能是低温解除自然休眠的原因。 相似文献
65.
日光温室黄瓜栽培CO_2浓度的消长规律初探 总被引:7,自引:0,他引:7
近 3a(年 )的研究结果表明 :日光温室内CO2 浓度有明显的季节变化和日变化。在整个生长期内因通风时间和通风量的不同 ,日光温室内的CO2 浓度 11月和 3月较高 ,5月较低。各时期日变化基本相同 ,但变化幅度因季节而异 ,上午随Pn的逐渐增大而下降 ,中午 12 :0 0~ 14 :0 0时降至最低 ,下午又随Pn的减小而缓慢回升。叶片的光合作用、呼吸作用和土壤呼吸是影响日光温室内CO2 浓度日变化的主要因素。有机肥施用量对室内土壤呼吸和日光温室CO2 浓度有较大影响 ,在有机肥充足的条件下 ,室内CO2 浓度基本满足黄瓜光合作用的需要 ,无须补施 ,如果在作物生长期间再定时随水向土壤中冲施有机肥 ,效果就更好 相似文献
66.
The effect of take-all root lesions on nitrate uptake of wheat was investigated in two experiments under controlled conditions. Plants were supplied with a nutrient solution labelled with 15 N during stem elongation and flowering to assess the distribution of the isotopic tracer in the different plant organs, and particularly in root segments located on both sides of take-all lesions. The 15 N atom percentage excess measured in root segments located below lesions longer than 1 cm was reduced on average by half compared with that in healthy roots and root segments above lesions, reflecting a reduction in nitrogen uptake by these root segments. This reduction probably resulted from the invasion and breakdown of phloem vessels by the fungus hyphae, interrupting energy supply and thus the uptake process. Severely infected plants showed an increase in the uptake rate per unit of efficient root, which appeared to be a compensatory response to reduction of efficient root biomass in order to satisfy shoot nitrogen demand. However, this compensatory response was insufficient to ensure nitrogen accumulation equivalent to that of healthy plants, as reductions in nitrogen accumulated in roots and aerial parts at flowering were up to 56 and 49%, respectively, for plants with more than 50% of the root system below lesions longer than 1 cm. 相似文献
67.
68.
J Petersen 《Weed Research》2003,43(1):33-39
Summary The experiments were carried out in the two spring barley fields of the organic six-course cattle:crop rotation at Foulumgaard, Denmark. The weed density was 300 and 1800 plants m−2 respectively. Pig slurry was applied by hand in microplots by four methods: broadcasting followed by incorporation, or injected in bands to depths of 5, 10 or 15 cm. Spring barley and weeds were sampled separately six times during the tillering and elongation phase of the spring barley. The effect of application method on dry-matter (d.m.) production, nitrogen uptake and recovery of applied nitrogen in the spring barley and the weeds is reported. Slurry banding halved the weed d.m. and weed N uptake compared with broadcasting, irrespective of weed density. Weeds recovered up to 12% of the applied nitrogen, which made them a significant competitor when the slurry was broadcast and incorporated. Banding by direct injection reduced the slurry:soil contact and the weed:crop competition balance for applied nitrogen moved in favour of the crop. Thus, the crop recovery of applied nitrogen at the end of the sampling period was increased from approximately 45% for broadcast and incorporated to approximately 50% for injected slurry, and coincidental weed recovery was reduced to a maximum of 5%. As the nitrogen supply normally affects plant d.m. production, banding of slurry might well improve crop competitiveness and its tolerance to mechanical weed control. 相似文献
69.
70.
通过室内与大田试验,明确O3对日光温室黄瓜病虫害的防治效果。研究结果表明,在病害发生初期,用O3发生器隔天施放O3,每天施放3次,每次40min,间隔20min,O3浓度维持约1mg/kg,施放后7天,对黄瓜霜霉病、白粉病、细菌性角斑病及灰霉病防治效果分别为63.4%、53.6%、68.7%和65.0%。室内施放O3 60s,间隔20min,持续12h,对温室白粉虱、南美斑潜蝇成虫杀伤力为100%。O3对黄瓜植株安全,但随着黄瓜生育期的推进,对O3的忍受能力依次减弱。苗期忍耐O3最大浓度为1~2mg/kg,结果期0.4~1mg/kg,后期0.2~0.4mg/kg。O3防治黄瓜病虫害新技术具有安全、有效、方便、无二次污染等特点,尤其在黄瓜定植前对棚室进行大剂量消毒,可不留死角,且有效地解决了硫磺等药物消毒所造成棚内一些设施的破坏及对生态环境污染的难题,具有广阔的推广应用前景。 相似文献