Inheritance and molecular mapping of restorer‐of‐fertility (Rf) gene in A2 hybrid system in pigeonpea (Cajanus cajan)     

Pankaj Sharma  Inderjit Singh  Asmita Sirari  Gaurav Khosla  Gurjeet Singh  Navkiran Kaur Ludhar  Sarvjeet Singh 《Plant Breeding》2019,138(6):741-747

Inheritance of fertility restorer gene in pigeonpea was studied using F₂ and BC₁F₁ populations derived from cross AL103A × IC245273. It was found to be controlled by single dominant gene. Out of 228 SSR primer pairs, 33 primer pairs showed parental polymorphism, while four primers were found polymorphic in bulk segregant analysis (BSA). These four primers viz., CcM 1615, CcM 0710, CcM 0765 and CcM 1522 were used for genotyping of F₂ population and were found to be placed at 3.1, 5.1, 28.1 and 45.8 cM, respectively. Two of them, CcM 1615 and CcM 0710, evinced clear and unambiguous bands for fertility restoration in F₂ population. The Rf gene was mapped on linkage group 6 between the SSR markers CcM 1615 and CcM 0710 with the distances of 3.1 and 5.1 cM, respectively. The accuracy of the CcM 1615 was validated in 18 restorers and six maintainer lines. The marker CcM 1615 amplified in majority of male restorer lines with a selection accuracy of 91.66%.  相似文献   

Alteration in important quality traits and antioxidant activities in Brassica oleracea with Ogura cybrid cytoplasm          下载免费PDF全文

Shyam S. Dey  Reeta Bhatia  Chander Parkash  Saharsh Sharma  Manish Dabral  Vibha Mishra  Ila Bhardwaj  Komal Sharma  Vinod Kumar Sharma  Raj Kumar 《Plant Breeding》2017,136(3):400-409

In vegetable brassicas, cytoplasmic male sterility (CMS) system is the most preferred mechanism for hybrid seed production and Ogura cybrid cytoplasm is the only source used widely. Effects of the alien cybrid cytoplasm on important quality traits need to be understood for their effective use in breeding programme. In analysing 38 Ogura cybrid cytoplasm‐based cauliflower CMS lines, it was revealed that introgression of Ogura cytoplasm resulted in significant alteration in important quality traits. Cupric reducing antioxidant activity and ferric reducing ability of plasma values were increased up to 5–10 times in different genotypes. Among 38 analysed CMS lines, concentration of plant pigments such as anthocyanin, total chlorophylls and ascorbic acid was reduced in 21, 17 and 32 genotypes, respectively. However, the concentration of total carotenoids and β‐carotene was elevated in most of the CMS lines and total carotenoid was increased up to 20 times in the CMS line, Ogu13‐85‐2A. The results indicate the role of nuclear–cytoplasmic interaction and mitochondrial genome in determining concentration of different quality traits.  相似文献   

细胞质因子对香菇交配型因子分离比例的影响          下载免费PDF全文

程水明  徐学锋 《华南农业大学学报》2007,28(1):18-21

主要通过双-单杂交技术,探讨造成香菇原生质体单核化后2种交配型单核体分离比例偏离1∶ 1与细胞质间的遗传关系.结果表明, 双-单杂交所形成的由受体细胞质和受体、供体细胞核组成的同质异核体经原生质体单核化裂解后,毫无例外地都是与受体交配型相同的核居多,说明受体细胞质环境对双-单杂交所得异核体裂解后的核的存活和数量起着重要的作用;双-单杂交中,作为供体的2个核中只有亲和力强的那个核优先迁移到受体中,形成新的异核体,即存在先导核,先导核与B因子的亲和特异性有关,特异性越高,先导核迁移速度越快.  相似文献   

利用分子标记鉴定萝卜细胞质雄性不育种质的研究     

谭婷婷  刘立锋  陈伟  林超  李化银  李利斌  王淑芬  高建伟  程斐 《山东农业科学》2011,(8):8-13

利用萝卜包含ORF138基因片段的Ogura胞质雄性不育（CMS）分子标记对收集的5份萝卜雄性不育系种子材料进行鉴定。结果表明,有1份种质为Ogura雄性不育材料;将Ogura分子标记应用子萝卜Ogura细胞质雄性不育基因的转育工作中,可以提高选择效率,缩短育种年限。其它4种萝卜种质的不育类型和相关遗传机制,有待于进一步鉴定和分析。  相似文献   

大白菜Ogura雄性不育系及保持系花药发育的细胞学观察   总被引：1，自引：0，他引：1  

王书强  刘俊英  崔辉梅 《新疆农业科学》2011,48(6):996-1000

[目的]研究3个大白菜Ogura雄性不育系及保持系花药小抱子发育过程.[方法]对3对大白菜Ogura雄性不育系及其保持系花药发育进行细胞学观察.[结果]3个大白菜Ogura雄性不育系(OBY,OBK,OD5)的小孢子败育均发生在单核早期,在开花前完全败育.[结论]不育系绒毡层液泡化早于保持系,花粉母细胞减数分裂时不育系绒毡层细胞已开始退化,而保持系(BY,BK,D5)绒毡层自然解体,供给小孢子发育所需的营养物质和发育空间.  相似文献   

甘蓝Ogura胞质雄性不育系发生同源异型转化的分子机制研究     

张强  张晓伟  原玉香  姚秋菊  赵艳艳  蒋武生  魏小春  王志勇 《河南农业科学》2015,(5)

为了揭示甘蓝Ogura胞质雄性不育系发生线粒体同源异型转化的分子机制,利用RT-PCR和转基因的方法分别进行了研究。 RT-PCR试验结果表明,甘蓝B基因AP3在雌蕊(心皮)发育初期异位高表达,C基因AG3在发生心皮化的雄蕊部位异位高表达,这可能导致了甘蓝Ogura胞质不育系线粒体同源转化的发生。而拟南芥转基因试验表明,线粒体定位表达载体p3301-ATP-ORF138能导致拟南芥雄性不育的发生,这说明orf138基因是发生线粒体同源异型转化的甘蓝Ogura胞质雄性不育系不育的决定因素。  相似文献   

玉米细胞质雄性不育系的鉴定     

徐莹莹  邓杰  王秋雪  于杰  王霞  赵伟  孙丽芳 《黑龙江八一农垦大学学报》2016,(5)

采用田间育性调查、花粉活力检测、特异引物PCR鉴定及细胞学形态观察等方法来研究新育成的雄性不育系DT-合344、ZT-合344的育性特点、胞质类型。结果表明,这两个不育系均为T型细胞质败育类型,该研究结果为进一步深入研究不育系的不育分子机理和生产应用奠定基础。  相似文献   

杂交水稻茎秆性状的胞质配合力及遗传力分析     

范存留  张玲  胡运高  杨国涛  唐力琼  廖倩  陈俊林 《贵州农业科学》2012,(8):1-4

为了研究不同不育胞质对杂交水稻茎秆性状的影响,选用生产上常用的5个杂交水稻不育胞质与5个高配合力恢复系,采用NCII模式研究株高及基部节间性状的亲本配合力、方差贡献率及遗传力。结果表明:杂交水稻胞质效应和恢复系效应在各性状中均达到显著或极显著差异水平,茎秆性状受基因型与环境互作效应的共同影响,重穗型和穗数型恢复系对各性状的一般配合力效应相差较大,JW型胞质基部节长的一般配合力效应值较野败型(WA型)低,而其直径和壁厚的一般配合力效应值则高于野败型胞质。结论:杂交水稻JW型胞质在保证茎秆充足的生物量积累的同时抗倒伏能力方面优于野败型胞质。  相似文献   

和胃保肝对建鲤肠绒毛高度和肝细胞的影响     

叶笃涵  陈强  刘庆华  陈炳华  张照红  甘平旭 《福建畜牧兽医》2010,32(6):1-4

挑选个体大小、体重相近(15g左右)的建鲤50尾,随机分为5组(G1、G2、G3、G4和G5),即基础料组(对照组)、0.1%和胃保肝添加组、0.2%和胃保肝添加组、0.4%和胃保肝添加组和0.8%和胃保肝添加组,每组2个重复,每个重复10尾建鲤。饲喂3周后取建鲤肠道和肝脏制成组织切片,测定肠绒毛高度。结果表明:0.1%、0.2%、0.4%、0.8%和胃保肝不同添加量试验组建鲤的肠绒毛高度都极显著高于对照组(P0.01),各试验组建鲤肝脏细胞的胞浆密度比对照组的大。在建鲤饲料中添加0.4%和胃保肝,促进建鲤肠绒毛增长和提高肝脏细胞胞浆密度效果最好。  相似文献   

Seed productivity test of CMS lines of Japanese bunching onion (Allium fistulosum L.) possessing the cytoplasm of a wild species, A. galanthum Kar. et Kir.     

Ken-ichiro Yamashita  Yosuke Tashiro 《Euphytica》2004,136(3):327-331

In a previous study, we developed male sterile lines of Japanese bunching onion (Allium fistulosum L.) possessing the cytoplasm of a wild species, A. galanthum Kar. et Kir., by backcrossing. To evaluate seed productivity of the male sterile lines in practise, they were crossed with the male fertile line, cultivar 'Kujyo', using honeybees as pollinators under field conditions. The number of florets and seeds per inflorescence, seed set and seed germination of the material were investigated. Although variation was observed among the male sterile lines, there were several lines having seed productivity equal to cultivar 'Kujyo'. Our data demonstrate that the male sterile lines of A. fistulosum possessing the cytoplasm of A. galanthum are useful as seed parents for the commercial F seed production of A. fistulosum.  相似文献