排序方式： 共有35条查询结果，搜索用时 15 毫秒
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运用免疫组化ABC法妊娠26～120d的奶山羊黄体中催产素免疫反应细胞的分布进行了观察。结果表明，奶山羊妊娠黄体中存在催产素(Oxytocin,OT）免疫反应阳性细胞。阳性细胞在形态上以卵圆形、圆形、棱形为主，还有一些具有明显的突起。根据阳性细胞胞质内反应颗粒着色的深浅，可把OT阳性细胞分为强阳性、中等阳性和弱阳性3种。在妊娠26～30d，阳性细胞数量最多，强阳性细胞主要分布于黄体的周边，中等阳性及弱阳性细胞则均匀分布于整个黄体组织中，妊娠31～60d，阳性细胞数量明显下降，弥散于整个黄体组织中；妊娠61～120d，阳性细胞的数量及逐渐增多，以中等阳性和弱阳性细胞为主，而强阳性细胞数量较少。连续切片HE染色的对照观察显示妊娠黄体中大、小黄体细胞均可出现OT免疫阳性反应。 相似文献
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Patients being treated for stress-related mental illness were observed during two summer programs in order to investigate the influence of multimodal nature-based therapy in a specially designed healing garden on patient recovery. The aim was to distinguish specific qualities, patterns and/or processes during the participants' stay in the healing garden. The study is a single-case study, using participatory observation. The data were coded following an inductive research process. The results showed that patients who underwent psychotherapy were more open and contact-seeking, and carried out extrovert recreational walks. By contrast, patients who underwent physiotherapy were introverted, emotionally withdrawn and performed introvert recreational walks. Interpretation of the data suggested that treatment combined with activities in certain parts of the rehabilitation garden induced and supported different psycho-physiological processes. The mechanisms and progress of these are discussed from the theory of situated cognition, and how these processes are stimulated and supported by characteristics in the garden. Oxytocin, a hypothalamic peptide which stimulates social interaction, induces anti-stress effects and stimulates growth and healing, may hypothetically be involved in these processes. 相似文献
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提出了一种开放式、模块化、分布式、可配置、可集成和可维护的基于多Agent的制造执行系统(MES)模型。首先分析了制造系统中的制造执行系统域，然后提出了基于多Agent的MES模型，采用UML图表详细分析了该模型的MES功能实现，最后给出了实施的MES系统框架。该MES系统易与企业资源计划(ERP)、车间控制系统(FCS)及其他制造系统相集成。 相似文献
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Takuya MURATA Kazumi NARITA Toru ICHIMARU 《The Journal of reproduction and development》2014,60(1):55-61
Estrogen action is mediated through several types of receptors (ERs), such as ERα, ERβ
and putative membrane ERs. Oxytocin receptor (OTR) and ER expression levels in the rat
uterus are regulated by estrogen; however, which types of ERs are involved has not been
elucidated. This study examined OTR, ERα and ERβ levels in ovariectomized rats treated
with 17β-estradiol (E2), an ERα agonist (PPT), an ERβ agonist (DPN) or estren (Es). E2 and
PPT increased OTR mRNA levels and decreased ERα and ERβ mRNA levels 3 and 6 h
posttreatment. DPN decreased ERα and ERβ mRNA levels at 3 and 6 h, while OTR mRNA levels
increased at 3 h and decreased at 6 h. OTR mRNA levels increased 3 h after the Es
treatment and then declined until 6 h. ERα and ERβ mRNA levels decreased by 3 h and
remained low until 6 h posttreatment with Es. The ER antagonist ICI182,780 (ICI)
suppressed the increases in OTR mRNA levels induced 3 h after the Es treatment. However,
ICI and tamoxifen (Tam) had no significant effect on ERα and ERβ mRNA levels in the
Es-treated or vehicle-treated group. In intact rats, proestrus-associated increases in OTR
mRNA levels were antagonized by both ICI and Tam. However, decreases in ERα and ERβ mRNA
levels were not antagonized by Tam and ICI, respectively. Therefore, uterine OTR gene
expression is upregulated by estrogen through the classical nuclear (or non-nuclear) ERs,
ERα and ERβ, while the levels of these ERs are downregulated by estrogen through multiple
pathways including Es-sensitive nonclassical ERs. 相似文献
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Sirotkin AV 《Veterinary journal (London, England : 1997)》2005,170(3):37-317
Recent data on the association between growth hormone (GH) and male and female reproductive processes, as well as the effects of GH on these processes and on some reproductive and non-reproductive disorders, and possible extra- and intracellular mediators of its action are reviewed. The available data suggest that GH is an important endocrine and autocrine/paracrine regulator of reproduction. It controls proliferation, apoptosis, growth and differentiation and the secretory and generative activities of different reproductive organs. It also regulates their response to gonadotrophin-releasing hormone (GnRH) and gonadotropins. Despite the effects of GH on the IGF/IGFBP (insulin-like growth factor binding protein) system, oxytocin, steroids, activin, gonadotropin and gonadotropin receptors, the majority of GH's actions on the reproductive processes are probably mediated not by these substances but by specific GH receptors acting through cAMP/protein kinase A, protein kinase G, tyrosine kinase-, MAP kinase and CDC2 kinase-dependent intracellular mechanisms. Although GH treatments can increase the risk of some reproductive and non-reproductive disorders, they may be useful in improving gonadal function, inducing superovulation and in embryo production. 相似文献
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XIE Chang-ning WU Jian WANG Xin-meng PENG Si-cong WU Jing XIAO Ling-hui LIU Tao 《园艺学报》2018,34(4):739
AIM: To investigate whether oxytocin has neuroprotective effects on hippocampal CA1 pyramidal neurons from neonatal rats exposed to hypoxic-ischemic brain injury and the underlying mechanisms. METHODS: An in vitro model of hypoxic-ischemic injury was used by exposing the brain slices to oxygen-glucose deprivation (OGD) solution. Acute dissociated brain slices (6~8 slices per rat) from 8 Sprague-Dawely rats of 7~10 d old were used. The slices were randomly divided into 4 groups:control group, OGD 20 min group, OGD 40 min group and OGD+oxytocin group. The effect of oxytocin on neuronal death was evaluated by TO-PRO-3 staining. Fresh brain slices from other 20 neonatal rats were divided into OGD group, OGD+oxytocin group, OGD+dVOT (oxytocin receptor antagonist)+oxytocin group, and OGD+bicucuclline (GABAA receptor antagonist)+oxytocin group. The onset of anoxic depolarization in the hippocampal neurons treated with different drugs was recorded by whole-cell patch-clamp techniques. RESULTS: The results of TO-PRO-3 staining showed that neuronal deaths in hippocampal CA1 area were increased over the prolonged OGD time. Oxytocin significantly reduced the hypoxic-ischemic deaths. Oxytocin dramatically prolonged the onset time of anoxic depolarization after the application of OGD solution. Both dVOT and bicuculline blocked this effect. CONCLUSION: Oxytocin plays a neuroprotective role in neonatal rat hippocampal CA1 pyramidal neurons by enhancing the inhibitory synaptic transmission via oxytocin receptors. Therefore, oxytocin is useful as a candidate for neuroprotective treatment after neonatal hypoxic-ischemic brain injury. 相似文献
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Chavatte-Palmer P Arnaud G Duvaux-Ponter C Brosse L Bougel S Daels P Guillaume D Clément F Palmer E 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2002,16(4):472-477
The induction of lactation is performed in ruminants by steroidogenic impregnation, followed by drugs intended to increase prolactin secretion. The aim of this study was to induce lactation in barren mares and to evaluate milk production. Five treated and 5 control mares were used in June and September in year 1, and 12 mares were used in year 2. Mares were administered a vaginal pessary (500 mg altrenogest and 50 mg estradiol benzoate) for 1 week. The 2nd week, another sponge with 100 mg estradiol benzoate was administered, together with 50 mg/100 kg body weight (BW) sulpiride in oil (IM q12h). All mares were milked by hand. Drug treatment was stopped after I L was obtained. Milk production and composition and plasma prolactin concentration were measured. In year 2, the same steroid treatment was applied, but mares received sulpiride (n = 6) or domperidone (1.1 mg/kg PO q12h) (n = 6). A milking machine and oxytocin injections 1 minute before the start of milking were used. In year 1, all treated mares started milking within 1-5 days after sulpiride treatment. Mean daily milk production was 0.88 +/- 0.52 L/500 kg BW. Milk immunoglobulin G (IgG) contents increased in all mares (IgG concentration range, 14-92 g/L). Plasma prolactin increased during sulpiride treatment (range. 27.7 +/- 2.9 to 43.7 +/- 6.7 ng/mL [before] to 289.0 +/- 7.8 ng/mL during treatment, P < .001). In year 2, results were similar to those in year 1, with peak IgG concentrations ranging from 4.2 to 106.7 g/L and a larger daily milk production (3.13 +/- 0.75 with sulpiride and 3.45 +/- 0.51 L/500 kg BW with domperidone). In conclusion, lactation can be induced in mares within 2 weeks, and some mares produce good-quality colostrum. 相似文献
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Dirk K. Vanderwall Desirée M. RasmussenKevin G. Carnahan PhD Tracy L. Davis PhD 《Journal of Equine Veterinary Science》2012
The objectives of this study were to (1) compare the effect of twice versus once daily administration of oxytocin on days 7-14 after ovulation on the duration of corpus luteum (CL) function and (2) determine the effect of oxytocin treatment on endometrial oxytocin receptor concentration in mares. In experiment 1, mares were randomly assigned to three groups on day 7: (1) untreated control group (n = 7), (2) twice daily oxytocin treatment group (n = 7), and (3) once daily oxytocin treatment group (n = 8). Oxytocin-treated mares received 60 U of oxytocin intramuscularly (IM) the respective number of times each day on days 7 through 14. One of seven control mares (14%), five of seven (71%) twice daily oxytocin-treated mares, and five of eight (63%) once daily oxytocin-treated mares had prolonged CL function. There was no significant difference in the proportion of mares with prolonged CL function between the two oxytocin-treated groups, and collectively, oxytocin treatment increased (P < .05) the proportion of mares with prolonged CL function compared with no treatment. In experiment 2, mares were randomly assigned to two groups (n = 5/group): (1) saline-treated control mares, and (2) oxytocin-treated mares. Beginning on day 7, control mares received 3 mL of sterile saline IM twice daily, and oxytocin-treated mares received 60 U of oxytocin IM twice daily through day 14. On day 15, endometrial oxytocin-binding capacity was determined (as a measure of oxytocin receptor concentration), and there was no difference (P > .1) between control and oxytocin-treated mares (1,465.7 ± 108 and 1,382.8 ± 108 fmol/mg protein [mean ± standard error of mean], respectively). 相似文献