Feasibility test of HBH Ⅱ compound microorganism on the reduction of septic tank nightsoil sludge      

SONG Zhen xi  WANG Li ao  GU Wei  HUANG Chuan  CHEN Da yong 《保鲜与加工》2009,(11):1339-1344

The paper selects septic tank nightsoil sludge as the study object, and conducts three treatments for nightsoil sludge anaerobic digestion reduction under 35 ℃ including CK treatment(without HBH II compound microorganism), T1 treatment(adding 0.005% HBH II compound microorganism in the original nightsoil sludge system) and T2 treatment(adding 0.005% HBH II compound microorganism after the nightsoil sludge was sterilized by pasteurism). It investigates the removal rate of total solid (TS), volatile solid (VS) and COD of the three treatments, and analyzes the main contents of nightsoil sludge before and after anaerobic digestion and the digestion efficiencies of them. The results indicate that the removal rates of TS, VS and COD of T1 are 46.03%, 49.96% and 47.06%, respectively, and there are 14.89%, 16.26% and 15.53% higher than CK, 6.63%, 8.02% and 11.90% higher than T2, respectively. There are significantly positive (p<0.01) relations between T1 and CK, and T1 and T2 in the removal rate of TS, VS and COD. The nightsoil sludge digestion efficiencies of CK, T1 and T2 are 34.69%, 51.09% and 43.05%, respectively and that of T1 significantly positive(p<0.01) differs from CK and T1 treatment. The results indicate that the corporate effect of the adding HBH II compound microorganism and the original microorganism of the nightsoil sludge system are helpful to the reduction of septic tank nightsoil sludge and it is feasible for directly adding HBH II compound microorganism to septic tank for the reduction of the nightsoil sludge in it.  相似文献   

烟草蛋白研究进展   总被引：1，自引：0，他引：1  

陈明达  丁玉  鞠明里  尹瑞卿  王飞 《河北农业科学》2009,13(6):50-52,128

从烟草中提取烟草蛋白,是解决目前动物蛋白紧缺问题的有效途径之一。介绍了烟草蛋白的组成与特性,分析了烟叶作为蛋白来源的潜力,综述了国内外烟草蛋白分离纯化技术的研究进展,讨论了影响烟草蛋白制备的主要因素,并对烟草蛋白的开发利用进行了展望。  相似文献   

两优培九剑叶衰老过程光合膜功能及蛋白质复合物的变化     

叶露幻  沈唯军  郑宝刚  宋涛  陈国祥  吕川根 《作物学报》2013,39(11):2030-2038

为探讨高产杂交稻两优培九在衰老过程中,剑叶光合膜蛋白质复合物的含量变化规律及其与光能吸收、转化、传递的关系,以大田栽培自然衰老剑叶为材料,利用活体叶绿素荧光动力学技术,并结合类囊体膜蛋白质复合物蓝绿温和胶电泳分析。结果表明,两优培九剑叶叶绿素含量、光合性能、类囊体膜蛋白稳定性等都在抽穗期达到顶峰,随后开始衰退,在扬花期、灌浆期尚保持较高水平,而进入籽粒成熟阶段衰退明显;随着衰老进程,光合膜蛋白质复合物有序非同步降解,稳定性为LHCII > PSIIcore > PSIcore > ATPase & Cyt b₆/f > LHCI;PSI和PSII蛋白和相应电子传递活性的稳定性及下降幅度差异较大;衰老过程叶绿素a/b的不断下降与相对于反应中心更稳定的捕光天线有关,剑叶生长后期LHCII维持高水平保持了叶片对光能的吸收,并可能在调节光系统间能量分布和协助过剩能量耗散中起重要作用。  相似文献   

西葫芦光系统Ⅱ耐热性研究     

赵传麟  王磊  刘瑞伟 《山东农业科学》2011,(10):26-31

选取耐热性不同的两个西葫芦品种明珠特早（不耐热）、绿波（耐热）,采用PEA荧光仪进行快速叶绿素荧光诱导曲线及其参数的测定。当温度在36—40℃时,两个品种的叶绿素荧光参数变化不明显。当温度高于44℃后,随着温度的增加,170缓慢上升,F-n、Fv和Fv／Fm大幅度下降,且明珠特早的变化幅度大于绿波。明珠特早在44％水浴15min时荧光动力学曲线在0．8ms处出现K峰,呈现O-K—J—I—P五峰型上升趋势,绿波晚出现10min。明珠特早在48℃水浴2min后出现K峰,绿波则在48℃水浴6min后出现K峰。随着热胁温度的升高,RC／CSo与Fvi／Fv的变化趋势恰好相反。  相似文献   

Resistance monitoring of Heliothis virescens to pyramided cotton varieties with a hydrateable, artificial cotton leaf bioassay     

Ana R. CabreraJaap Van Kretschmar  Jack S. BachelerHannah Burrack  Clyde E. SorensonR. Michael Roe 《Crop Protection》2011,30(9):1196-1201

Proof of concept was demonstrated for a practical, off-the-shelf bioassay to monitor for tobacco budworm resistance to pyramided Bt cotton using plant filtrates. The bioassay was based on a previously described feeding disruption test using hydrateable artificial diet containing a blue indicator dye, a diagnostic dose of insecticide and novel assay architecture. Using neonate larvae from a Bt-susceptible, laboratory reared tobacco budworm strain, a diagnostic dose for Bollgard II and WideStrike cotton was obtained that limited neonate blue fecal production to 0-2 pellets in 24 h (Bt-resistant larvae produced >2 fecal pellets). The bioassay was tested with three different field populations of tobacco budworm collected from tobacco in central North Carolina (USA) and shown to accurately diagnose susceptibility to Bt. The diagnostic doses were also successfully evaluated with two Bt-resistant, laboratory reared tobacco budworm strains. Shelf life studies showed the assay could be stored for at least 6 months at room temperature (longer storage times were not studied). The application of the bioassay as an easy to use monitoring tool is discussed.  相似文献   

Chlorophyll Fluorescence,Photosynthetic and Morphological Characteristics of Waterlogged Sesame Seedlings     

Fengying XU  Xiaoling WANG  Xiurong ZHANG 《农业科学与技术》2017,18(4)

Two sesame accessions, ZZM2541 and Ezhi-2, with different tolerance to waterlogging were selected, and the seedlings at the 4-true leaf stage were treated by waterlogging for 48 h. Growth parameters were evaluated 1 d before the beginning of waterlogging and on the 3rd day after the removal of waterlogging (DARW). Morphological characteristics of root and chlorophyll fuorescence were measured on the 3rd DARW and leaf gas exchange was measured on the zero, 3rd and 15th DARW. Results showed that sesame accessions ZZM2541 and Ezhi-2 responded to waterlogging in considerably different performance.The stress induced leaf chlorosis and abscission and slowed growth of plant height in both accessions, but symptom occurred seriously in the susceptive Ezhi-2. In the more tolerant ZZM2541, plentiful of adventitious roots formed above the level.All of the average values of maximum fuorescence yield (Fm), quantum efficiency of open PSII centres (Fv/Fm), initial fluorescence (Fv/F0) and chlorophyll content (Chl) decreased at the 4-true leaf stage in both accessions after suffering to the stress.The decreases of Fm, Fv/F0 and Chl were more pronounced in Ezhi-2 than in ZZM2541. Less reductions of mean photosynthetic rate (Pn), transpiration rate (Tr), and stomatal conductance (gs) were observed in the leaves of waterlogged ZZM2541 than in waterlogged Ezhi-2 (compared to controls), and the leaves of Ezhi-2 showed a higher water use efficiency (WUE) after the removal of waterlogging. Based on the results, it was concluded that the tolerance to waterlogging of ZZM2541 appears to depend on a combination of photosynthetic characteristics responses and morphological adaption.  相似文献   

大蒜素治疗细菌感染所致呼吸机相关性肺炎的临床研究          下载免费PDF全文

闫芳  陈倪  杨芳  张毅  张静  王平 《勤云标准版测试》2017,37(3):313-316

目的 评估大蒜素治疗细菌感染所致呼吸机相关性肺炎危重患者的临床疗效。方法 采用前瞻性随机对照研究方法,选取2011年01月-2014年12月收入重症监护病房且符合呼吸机相关性肺炎诊断标准的92例患者（均为细菌感染所致）,按照随机数字表法将其分为治疗组42例及对照组50例。对照组在常规治疗基础上加用抗生素治疗,治疗组在对照组基础上加用大蒜素治疗,疗程14 d。分析两组患者的病原菌分布情况,比较两组患者机械通气时间、抗生素治疗时间、住ICU时间及14 d病死率;并观察记录大蒜素使用过程中安全性及不良反应发生率。结果 细菌感染所致呼吸机相关性肺炎患者的常见病原菌为鲍曼不动杆菌（35.2%）、金黄色葡萄球菌（17.1%）、铜绿假单胞菌（13.3%）、肺炎链球菌（13.3%）及肺炎克雷伯菌（8.6%）。治疗组机械通气时间略短于对照组（P>0.05）,抗生素使用时间及住ICU时间显著短于对照组（P<0.01）。两组患者治疗后14 d病死率无统计学差异（P>0.05）。结论 细菌感染所致呼吸机相关性肺炎患者在常规抗感染治疗基础上加用大蒜素治疗,能显著缩短抗生素使用时间,且有缩短患者机械通气时间及住ICU时间,临床不良反应发生率低,可以安全使用。  相似文献   

Kinetics of Hg adsorption onto noncrystalline Al hydroxide as influenced by low-molecular-weight organic ligands     

Jae E. Yang  Yong Sik Ok 《Archives of Agronomy and Soil Science》2017,63(1):124-135

This study was conducted to investigate the kinetics of Hg adsorption by noncrystalline Al hydroxide as influenced by various pH conditions and cysteine (cys), glycine (gly), and citric acid (cit), which have different structures and functionalities, as low-molecular-weight organic ligands using the GEOCHEM-PC software. The influence of these organic ligands on the kinetics of Hg adsorption varied according to their concentration, structure, and functionality and pH. The adsorption of Hg followed multiple first order kinetics with initial rapid adsorption, followed by slow adsorption. Cysteine suppressed or enhanced Hg adsorption, depending on pH and its concentration. Glycine and citric acid exerted suppressing and enhancing effects, respectively, with the exception of at pH 4.5 and at lower concentrations, at which no influence was observed as compared to the control. Two mechanisms were thought to mediate the adsorption of Hg(II); specific surface complexation in the control, cysteine and glycine systems, and ligand exchange in the citric acid system. The Hg adsorption at all levels of organic ligands decreased with increasing pH, with the exception of at the higher concentration of cysteine, at which the reverse trend was observed. The influence of organic ligands on the dynamics of Hg in the freshwater environment merits further study.  相似文献   

基于CO II全基因的差翅亚目昆虫分子系统学研究     

张晓洁  查岭生 《安徽农业科学》2010,38(5):2264-2267

[目的]对基于CO Ⅱ全基因的差翅亚目昆虫的分子系统学进行研究。[方法]设计蜻蜓目昆虫CO Ⅱ全基因的非特异性引物,获得差翅亚目5科6属8种共8条CO Ⅱ全序列。采用CluxtalX1.8、ContigExpress、MEGA2.1、PHYLIP3.6a以及MrBayes V3.0等生物学软件及简约法和最大似然法对其进行分析并构建分子系统树。[结果]CO Ⅱ的A＋T含量较低（68.6%）,说明蜻蜓目是一比较原始的类群;每条序列均含有2个半胱氨酸,这一点有别于其他类群昆虫;不支持将大蜻亚科上升为科的观点;支持将大蜓科和蜓科归入蜓总科,而将春蜓科上升为总科的观点;差翅亚目5科之间的进化关系由慢到快依次为：春蜓科→大蜓科→蜓科→伪蜻科→蜻科。[结论]为蜻蜓目分子系统学研究提供相关依据。  相似文献   

Evaluation of type I fusarium head blight resistance of wheat using non-deoxynivalenol-producing fungi     

N. Gosman†  Srinivasachary  A. Steed  E. Chandler  M. Thomsett  P. Nicholson 《Plant pathology》2010,59(1):147-157

A series of experiments was conducted to determine whether type I resistance (resistance to initial infection) to fusarium head blight (FHB) in wheat could be assessed using fungal species/isolates that do not produce deoxynivalenol (DON), a mycotoxin critical to the spread of Fusarium graminearum in the wheat spike. It was shown that, while the non-toxin-producing species Microdochium nivale and M. majus could infect following spray inoculation of wheat spikes, they were unable to spread within the spike following point inoculation. However, although these species might reveal type I resistance, they are not highly pathogenic towards wheat. A nivalenol (NIV)-producing isolate of F. graminearum caused high levels of disease following spray inoculation, but spread only very slowly within the spike and rarely induced bleaching above the point of inoculation. It is proposed that spray inoculation with an appropriate, aggressive, non-DON-producing FHB pathogen may be used to characterize type I resistance to complement point inoculation with a DON-producing isolate to assess type II resistance (resistance to spread within the spike).  相似文献