越橘基因组DNA的快速提取及分析   总被引：4，自引：0，他引：4  

徐娜  夏秀英  徐大可  冯雪松 《果树学报》2007,24(5):714-717

为了从富含多酚、多糖及色素的越橘叶片中提取适用于分子生物学研究的高质量基因组DNA。以越橘幼叶为实验材料,比较了CTAB、SDS、高盐低pH值3种提取方法,获得了一种以CTAB法为基础的分离高质量完整DNA的简便、快速方法。用紫外分光光度计、琼脂糖凝胶电泳、RAPD-PCR、酶切等方法对获得的DNA进行了分析,结果表明,快速CTAB法所提取的DNA产量高、质量好,完全能够满足RAPD、PCR等分子生物学实验的要求。  相似文献   

金脉单药花组培工厂化技术研究   总被引：1，自引：0，他引：1  

李永文  李红 《北方园艺》2007,(7):179-181

选用金脉单药花健壮枝条作为材料,进行组培工厂化育苗繁殖技术研究.结果表明:愈伤组织诱导激素组合以6-BA0.5mg/L NAA 0.2mg/L最为适合,愈伤组织的生长速度和质量均满足要求,继续培养可分化产生大量不定芽;继代培养采用6-BA 0.3 mg/L NAA 0.1mg/L,增殖倍率控制在6～8倍;生根培养采用NAA 0.5 mg/L IBA 0.1 mg/L,一般15d左右生根,平均根量5～6条,生根率100%,移栽成活率高,满足植物组织培养工厂化育苗技术要求.  相似文献   

东北刺人参组培快繁的可行性研究   总被引：1，自引：0，他引：1  

朴炫春  廉美兰  刘继生  金国振 《延边大学农学学报》2006,28(1):10-13

在MS培养基中加入BA1．0mg／L时可产生3个健壮的丛生苗。采用组织培养技术快速繁殖刺人参幼苗具有可行性，这对保护和开发濒危的刺人参资源具有极其重要的意义．  相似文献   

食用仙人掌的离体培养及其快速繁殖   总被引：15，自引：1，他引：14  

陈丽静  潘英  马慧  刘少霞  姜明兰  钟文田 《园艺学报》2001,28(4):327-330

 研究了食用仙人掌离体快繁的若干影响因素。适宜的培养基为: 不定芽诱导, MS + 6-BA 3. 0 mg/L + NAA 0. 1 mg/L ; 继代和增殖, MS + 6-BA 1 mg/L + KT 0. 5 mg/L + NAA0. 3 mg/L , 继代培养30 d , 平均增殖倍数为8 ; 生根培养基为1/ 2 MS + NAA 0. 1 mg/L + IBA 0. 1 mg/L。在培养过程中, 有些外植体膨大, 并有疏松的淡黄色颗粒状愈伤组织出现, 从愈伤组织表面分化出芽; 有些外植体不膨大, 无愈伤组织出现, 直接分化出丛生芽。过渡移栽基质为田园土∶珍珠岩∶河沙= 1∶1∶1 , 试管生根苗根长约1 cm时移栽, 成活率最高可达95 %以上。  相似文献   

金合欢组织培养和快速繁殖的研究   总被引：14，自引：0，他引：14  

翟建中  顾梅俏  程荣昌  张燕  刘小军 《园艺学报》2001,28(2):149-152

 采用金合欢(Acacia dealbata) 大树顶芽、幼苗茎尖、种子小苗上胚轴和幼叶作外植体进行组培和快繁研究。结果表明: 用MS + 6-BA 6 mg/L + NAA 0. 1 mg/L + NaH₂PO₄ 30 mg/L 可提高愈伤组织的出芽率, 有利于扩大繁殖系数, 而用MS + 6-BA 2 mg/L + NAA 0. 1 mg/L + NaH₂PO₄ 30 mg/L 可以增加发枝率。珍珠岩+ 泥炭(1∶1) 基质可促进小苗后期生长,加速成苗。  相似文献   

Vegetative propagation studies of gum arabic trees. 1. Propagation of Acacia senegal (L.) Willd. using lignified cuttings of small diameter with eight nodes     

S. Badji  I. Ndiaye  P. Danthu  J. P. Colonna 《Agroforestry Systems》1991,14(3):183-191

In order to study and monitor rates of rooting, callusing and survival, small diameter stem cuttings (less than 0.4 cm) with eight nodes (12–14 cm long) were taken from trees in the field at different times of the year in tests with small batches of cuttings. Among the four tested substrates, well drained crushed basalt powder placed on a bed of gravel was the best rooting medium. Rooting was generally poor or even non-existent with this type of cutting, but the presence of leaves both promoted rooting and significantly (using Chi square test) improved survival of the cutting. Four leaves were sufficient to assure the necessary metabolic functions. Only stem cuttings collected during the rainy season gave roots and then, only when hormonal treatments were applied to the cuttings. 8%-IBA resulted in significantly better rooting than did 2%-IBA, 0.2%-NAA and 1%-IAA. With 8%-IBA, the rate of rooting varied between 50 and 70% for leafy cuttings collected in the rainy season. Hormonal treatments had a significant effect on the survival rate of the stem cuttings during the two months observation period. Further investigations are being conducted with different types of cuttings.  相似文献   

希蒙得木研究综述   总被引：1，自引：0，他引：1  

曹兵  高捍东 《福建林业科技》2002,29(3):88-91

综述了希蒙得木的栽培利用历史、种子油的化学性质与应用、生物学特性、繁殖与栽培技术等 ,并提出今后的研究重点。  相似文献   

花尾胡椒鲷的人工繁殖   总被引：8，自引：0，他引：8  

周仁杰  黄斌  林涛 《上海水产大学学报》2000,9(2):103-107

报导了１９９９年５月１３日至７月５日进行的花尾胡椒鲷人工育苗技术研究。培育获得平均全长２４．３ｍｍ、平均体重０．２９ｇ的幼鱼鱼苗３．０万尾,育苗成活率达４９．２％。试验期间室内育苗池水温与盐度的自然变化范围分别为２４．０￣３０．５℃,２９．６￣３０．５。仔、稚、幼鱼培育的饵料系列基本搭配为轮虫－卤虫无节幼体和桡足类、海水枝角类－鱼肉糜。试验发现：花尾胡椒鲷人工育苗的死亡高峰期出现在稚鱼后期,３８￣４４日龄阶段。  相似文献   

Loblolly pine cutting morphological traits: Effects on rooting and field performance     

Foster  G.S.  Stelzer  H.E.  McRae  J.B. 《New Forests》2000,19(3):291-306

Shoot cuttings were harvested fromfour-year-old, loblolly pine hedges in March andSeptember of 1987, and placed into a series offactorial combinations of cutting length, diameterclass, and the presence/absence of a terminal bud toassess effects on rooting and field performance.Average rooting in the March trial was 50 percent andonly 20 percent for the September trial; however, thebest treatment in March yielded 100 percent rooting.Terminal bud status did not appear to influencepercent rooting. Shorter cuttings (5.1 or 7.6 cm)with an average diameter of 2 or 3 mm tended to rootbetter and develop more roots. Field performance ofthe rooted cuttings through age five suggests that theoriginal cutting does not require a terminal bud, butthe best set of morphological traits differ dependingon bud status. Considering both rooting ability andfield growth with an original tip bud present, thebest cutting dimensions were 5.1 or 7.6 cm long and 2or 3 mm in diameter. Without a tip bud present,cutting dimensions were restricted to 7.6 or 10.2 cmlong and 3 mm in diameter. Number of main roots wasa weak predictor of tree height or dbh at age five.  相似文献   

苦水玫瑰分枝和生根特性研究     

薛智德 《陕西林业科技》2000,(1):12-14

本文通过挖根,对一年生苦水玫瑰地上茎,地下茎和根的分枝特点及生根特性进行了详细调查,结果表明三个部位的第一、二次枝数差异均显著（或极显著）,且以地上茎生枝数量最多,玫瑰根系属浅根吸收根密集型。并据此提出了２／３强度压条的数量和变幅,分枝繁殖的数量和变幅。  相似文献