Cytokine profiles in peripheral blood mononuclear cells from patients with cystic echinococcosis     

《Comparative immunology, microbiology and infectious diseases》2020

IntroductionCystic echinococcosis (CE) is a chronic zoonotic disease caused by the larval stage of Echinococcus granulosus (E. granulosus), which affects domestic and wild carnivores as the definitive host and ungulates as intermediate hosts. In intermediate hosts, both Th1 and Th2 cells are involved in the immune responses to an echinoccocal infection. This study aimed to investigate production of IL-4, IL-10, and IFN-γ cytokines in peripheral blood mononuclear cells (PBMCs) of CE patients before and after surgical treatment.MethodsTo evaluate cytokine production in response to E. granulosus antigens, we investigated IL-4, IL-10, and IFN-γ production in PBMCs of 20 CE patients in response to hydatid cyst fluid antigen (HCF-Ag) before and after surgical treatment using ELISA.ResultsThe mean IL-4 production from HCF-Ag stimulated PBMCs was significantly decreased (p < 0.05), while IFN-γ was significantly increased in HCF-Ag stimulated PBMCs in patients after surgery (p = 0.005).Furthermore, our results showed that there is no significant difference between IL-10 production in patients before and after treatment (p = 0.562).ConclusionsOur data Indicated production of IL-4 in cultured PBMCs of CE patients stimulated with HCF-Ag was decreased significantly. While, production of IFN-γ was increased significantly in responses to HCF Ag after surgery. We concluded that the evaluation of IL-4 and IFN-γ in HCF-Ag stimulated PBMCs of CE patients should be considered as a useful marker in the follow up of patients with cystic echinococcosis.  相似文献   

密度和播期对杂交玉米遵糯4号鲜穗数的影响     

伍名龙  龙正凯  舒中兵  段明禹 《现代农业科技》2015,(12):14-15

采用206最优回归设计方案,研究了不同密度和播期对遵糯4号鲜穗数的影响。结果表明:在试验设置的密度和播期范围内,随二因素升高,鲜穗数先升后降。综合考虑,密度在55 500~64 500株/hm2、播期在4月12—18日水平区间内,有利于遵糯4号鲜穗数的提高。  相似文献   

基于Cortex-M4内核的μCOS-Ⅱ移植     

《科技视界》2016,(9)

针对TI公司新推出的基于Cortex-M4内核的TM4C123G高性能低功耗芯片,详细介绍了嵌入式开源实时操作系统μCOS-Ⅱ在芯片上的移植方法。根据移植的需求,首先介绍了芯片的一些基本功能以及相关的软件开发环境,然后结合芯片的固有特性以及μCOS-Ⅱ移植的需求,使用C语言和汇编语言修改了相关的源文件,并详细阐述了修改的原因。  相似文献   

复杂疾病的遗传咨询技术     

张堃  裴雨晨  杨进  董靖 《广西农业生物科学》2014,(4):916-919

复杂疾病大面积爆发使人们的生活健康面临着重大挑战。在这一环境下,遗传咨询在人们的生活中占据了越来越重要的地位。依据基因与环境互作共同影响个体健康的原理,咨询者在遗传咨询师的指导帮助下合理解决各种遗传学问题可以有效降低复杂疾病的发病率。目前国内的遗传咨询发展尚处于萌芽期,随着遗传咨询在健康领域的地位上升,该技术在未来将会进一步推广应用。  相似文献   

大豆胞囊线虫主效抗病基因Rhg4(GmSHMT)的CAPS/dCAPS标记开发和利用     

史学晖  李英慧  于佰双  郭勇  王家军  邱丽娟 《作物学报》2015,41(10):1463-1471

大豆胞囊线虫(soybean cyst nematode,SCN)严重危害世界大豆生产,Rhg4(resistance to Heterodera glycines 4)是控制大豆SCN抗性的2个主效位点之一。本研究针对Rhg4(Gm SHMT)上的2个单核苷酸多态性(single nucleotide polymorphisms,SNP)位点开发了快速、经济、简便易行的CAPS(Rhg4-389)和d CAPS标记(Rhg4-1165),并用开发的2个标记鉴定了以大豆胞囊线虫应用核心种质为主的193份代表性抗感种质。结果表明,Rhg4-389和Rhg4-1165位点间存在显著连锁不平衡(P=0.0001,r2=0.87),可形成4种单倍型。Rhg4-389-G/Rhg4-1165-T和Rhg4-389-C/Rhg4-1165-A为优势单倍型,稀有单倍型Rhg4-389-G/Rhg4-1165-A和Rhg4-389-C/Rhg4-1165-T是在中国抗源中新发现的单倍型。结合193份种质对SCN 3号小种抗性鉴定分析发现,Rhg4-389-G和Rhg4-1165-T主要存在于抗病种质,它们形成的单倍型对抗病种质鉴定效率可达94.1%。本研究开发了可用于辅助大豆SCN抗性鉴定且方便育种家利用的CAPS/d CAPS标记,且用其摸清了应用核心种质等重要抗源在Rhg4位点的"本底",为育种家有效利用这些优异抗源提供了重要信息。  相似文献   

热应激下鸡肝脏SFRP1和PDK4基因表达水平研究     

赵妤娟  许生友  姜润深  曲鲁江 《中国家禽》2012,34(15):12-15

为研究热应激条件下,鸡SFRP1和PDK4两个与代谢相关的基因在鸡肝脏组织中的差异表达,选取12只60日龄体重相近的黄羽肉母鸡,分为正常对照组(CL)、热应激反应组(HS)和热应激反应后的降温组(HF)3个处理组,并对这两个基因在三组肝脏组织中的表达水平进行荧光定量表达分析。结果表明,在热应激状态下,SFRP1和PDK4基因在肝脏中的表达均上调,且PDK4在对照组和热应激组间差异显著(P<0.05)。本研究结果还表明,当热应激反应后温度重新降至(25±1)℃时,SFRP1和PDK4在肝脏中的相对表达量均有不同程度的恢复,进一步说明这两个基因在热应激中发挥的作用。本研究为从分子机制分析热应激提供了相应的参考。  相似文献   

用2,4-D处理获得可育甘薯组种间杂种   总被引：4，自引：0，他引：4  

翟红  刘庆昌 《农业生物技术学报》2002,10(2):113-115,T001

用50mg/L 2,4-D处理杂交不亲和组合甘薯(Ipomoea batatas (L.)Lam.)品种徐薯18(2n＝6x＝90)×L.lacunosa(2n＝2x＝30)以及I.trifida(2n＝4x＝60)×L triloba(2n＝2x＝30)的母本花器,获得了可育种间杂种植株,并对种间杂种进行了过氧化物酶同工酶分析和细胞学观察.特别是徐薯18×I.lacunosa的杂种后代具有明显的结薯性,这是首次报道获得具有结薯性的甘薯品种和第Ⅱ群近缘野生种种间杂种.  相似文献   

microRNA-146a通过靶向MAPK4和Myosin Va基因调控羊驼黑色素细胞增殖、迁移     

刘学贤  杜斌  郭湘  薛骥轩  于雷涛  范瑞文 《畜牧兽医学报》2021,52(4):967-975

旨在研究microRNA-146a(miR-146a)对羊驼黑色素细胞增殖和迁移的调控及其分子机制。本研究使用双荧光素酶试验验证MAPK4和Myosin Va是miR-146a的靶基因;利用荧光定量PCR和蛋白质免疫印迹试验检测在羊驼黑色素细胞中过表达miR-146a对相关下游基因表达的影响;利用CCK8和Transwell检测miR-146a过表达对羊驼黑色素细胞增殖和迁移的影响。结果显示,与对照组相比,将miR-146a和MAPK4或Myosin Va共转染293T细胞,双荧光素酶活性分别极显著下降36%和30%(P<0.001);MAPK4和Myosin Va基因转录水平分别极显著下调67%和47%(P<0.001,P<0.01);蛋白质水平的表达量分别显著或极显著下调38%和69%(P<0.05,P<0.01);增殖和迁移相关的基因CREB、MITF、MLPH和Rab27a在转录水平和蛋白水平的表达均极显著下调(P<0.01,P<0.001);CCK8和Transwell结果显示,过表达miR-146a使羊驼黑色素细胞的增殖和迁移能力极显著下调(P<0.01)。综上所述,miR-146a通过靶向调控MAPK4和Myosin Va,使增殖和迁移相关的基因MEK1、CREB、MITF、MLPH和Rab27a的表达下调,从而对羊驼黑色素细胞的增殖和迁移起抑制作用。  相似文献   

AR regulates porcine immature Sertoli cell growth via binding to RNF4 and miR-124a     

Xinpeng Yang  Yue Feng  Yang Li  Dake Chen  Xuanyan Xia  Jialian Li  Fenge Li 《Reproduction in domestic animals》2021,56(3):416-426

Sertoli cells are the only somatic cells in the seminiferous epithelium which directly contact with germ cells. Sertoli cells exhibit polarized alignment at the basal membrane of seminiferous tubules to maintain the microenvironment for growth and development of germ cells, and therefore play a crucial role in spermatogenesis. Androgens exert their action through androgen receptor (AR) and AR signalling in the testis is essential for maintenance of spermatogonial numbers, blood–testis barrier integrity, completion of meiosis, adhesion of spermatids and spermiation. In the present study, we demonstrated that AR gene could promote the proliferation of immature porcine Sertoli cells (ST cells) and the cell cycle procession, and accelerate the transition from G1 phase into S phase in ST cells. Meanwhile, miR-124a could affect the proliferation and cell cycle procession of ST cells by targeting 3′-UTR of AR gene. Furthermore, AR bound to the RNF4 via AR DNA-binding domain (DBD) and we verified that RNF4 was necessary for AR to regulate the growth of ST cells. Above all, this study suggests that AR regulates ST cell growth via binding to RNF4 and miR-124a, which may help us to further understand the function of AR in spermatogenesis.  相似文献   

Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen     

Hiroshi BANNAI  Manabu NEMOTO  Koji TSUJIMURA  Takashi YAMANAKA  Ken MAEDA  Takashi KONDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):309-311

To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4 (EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer: MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more strongly to the gG4-24-mer peptide than to the gG4-12-mer peptide. We used peptide ELISAs to test paired sera from horses naturally infected with EHV-4 (n=40). gG4-24-mer ELISA detected 37 positive samples (92.5%), whereas gG4-12-mer ELISA detected only 28 (70.0%). gG4-24-mer ELISA was much more sensitive than gG4-12-mer ELISA.  相似文献