Vitamin D Status in Different Stages of Disease Severity in Dogs with Chronic Valvular Heart Disease          下载免费PDF全文

T. Osuga  K. Nakamura  T. Morita  S.Y. Lim  K. Nisa  N. Yokoyama  N. Sasaki  K. Morishita  H. Ohta  M. Takiguchi 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2015,29(6):1518-1523

Background

In humans with heart disease, vitamin D deficiency is associated with disease progression and a poor prognosis. A recent study showed that serum 25‐hydroxyvitamin D [25(OH)D] concentration, the hallmark of vitamin D status, was lower in dogs with heart failure than in normal dogs, and a low concentration was associated with poor outcome in dogs with heart failure.

Objectives

To elucidate the vitamin D status of dogs with chronic valvular heart disease (CVHD) at different stages of disease severity.

Animals

Forty‐three client‐owned dogs with CVHD.

Methods

In this cross‐sectional study, dogs were divided into 3 groups (14 dogs in Stage B1, 17 dogs in Stage B2, and 12 dogs in Stage C/D) according to ACVIM guidelines. Dogs underwent clinical examination including echocardiography. Serum 25(OH)D concentrations were measured in each dog.

Results

Serum 25(OH)D concentration was significantly lower in Stage B2 (median, 33.2 nmol/L; range, 4.9–171.7 nmol/L) and C/D (13.1 nmol/L; 4.9–58.1 nmol/L) than in Stage B1 (52.5 nmol/L; 33.5–178.0 nmol/L) and was not significantly different between Stage B2 and Stage C/D. Among clinical variables, there were significant negative correlations between 25(OH)D concentration and both left atrial‐to‐aortic root ratio and left ventricular end‐diastolic diameter normalized for body weight.

Conclusions and Clinical Importance

These results indicate that vitamin D status is associated with the degree of cardiac remodeling, and the serum 25(OH)D concentration begins to decrease before the onset of heart failure in dogs with CVHD.  相似文献   

Expression of Cdc25a in cross-species hepatocellular carcinoma tissues and its significance     

LU Xiao-xu  CAO Ji  LI Yuan  SUN Wen  ZHU Ling-qun  YANG Chun  SU Jian-jia 《园艺学报》2014,30(5):820-824

AIM：To study the expression of cell division cycle 25a protein (Cdc25a) in hepatocellular carcinoma (HCC) tissues of different species, including human, rat and tree shrew, and to verify the feasibility of the strategy of cross-species oncogenomics screening. METHODS：Real-time fluorescence quantitative PCR and Western blotting were applied to detect the expression of Cdc25a at mRNA and protein levels in the HCC tissues, corresponding HCC-adjacent liver tissues and normal liver tissues collected from humans, rats and tree shrews. RESULTS：The mRNA expression of Cdc25a in the HCC tissues of humans, rats and tree shrews was higher than that in normal liver tissues (P<0.05). The mRNA levels of Cdc25a in the HCC tissues of humans and rats were higher than those in the corresponding HCC-adjacent liver tissues (P<0.05). The mRNA expression of Cdc25a in the HCC tissues was significantly correlated with the portal vein tumor thrombus, the extrahepatic metastasis and the clinical stage, but was not correlated with the recurrence of tumor, the diameter of tumor, the number of tumor, the level of serum alpha-fetoprotein and the differentiation of tumor. The protein levels of Cdc25a in the HCC tissues of humans, rats and tree shrews were higher than those in the corresponding HCC-adjacent liver tissues and the normal liver tissues. CONCLUSION：Cdc25a may be a particularly crucial molecule for hepatocarcinogenesis. The cross-species oncogenomics screening may represent a feasible and convenient way for identifying key molecules of human HCC.  相似文献   

Effects of RNA interference targeting CDC25agene on proliferation of human liver cancer HepG2 cells     

LI Wei  CAO Ji  ZHOU Ling-li  LUO Wang  YANG Chun  LUO Cheng-piao  LI Yuan  SU Jian-jia 《园艺学报》2014,30(12):2142-2147

AIM: To investigate the effect of silencing cell division cycle 25a (CDC25a) gene on the proliferation of human hepatoma HepG2 cells. METHODS: CDC25agene in human hepatoma HepG2 cells was silenced by RNA interference. Real-time PCR was applied to detect the expression of CDC25a, cyclin E and CDK2 at mRNA levels in the HepG2 cells. Western blotting was applied to detect the expression of CDC25a at protein level. In addition, MTT assay, Giemsa staining and flow cytometry were used to measure the proliferation of human hepatoma HepG2 cells. RESULTS: The expression of CDC25a at mRNA and protein levels in RNA silence group was lower than those in negative control group and normal control group (P＜0.05). The mRNA expression of cyclin E and CDK2 in silence group was lower than that in negative control group and normal control group (P＜0.05). The cell proliferation in silence group was lower than that in negative control group and normal control group (P＜0.05). The results of flow cytometry revealed that the cells in silence group were blocked in G1 phase. CONCLUSION: Infection of LV-CDC25a-RNAi recombinant to the HepG2 cells effectively inhibits the CDC25agene expression and the proliferation of human hepatoma cells, and arrests the cells in G1 phase, suggesting that CDC25agene may be a key target for the treatment of liver cancer.  相似文献   

纤维素降解真菌A25-2的分离、鉴定及其产纤维素酶的酶学特性     

苏杨  张政  杨齐  刘君梁  段承杰  冯家勋 《广西农业生物科学》2010,(3):427-433

本研究以Avicel-刚果红选择培养基为初筛培养基,从云南哀牢山国家级自然保护区和广西猫儿山国家级自然保护区的土壤样品中分离筛选得到4200株真菌,从中筛选出透明圈与菌落直径比较大、透明程度较为清晰的12个菌株。通过液体培养发酵,测定其上清液中的羧甲基纤维素酶活力、滤纸酶活力和Avicel酶活力,最终筛选出一株产该三种酶且其活力均最高的真菌菌株A25-2。通过对菌株A25-2形态学观察和其内转录间隔区（internal transcribed spacer,ITS）序列同源性比对分析,将菌株A25-2鉴定为哈茨木霉（Hypocrea lixii）。酶活测定结果表明菌株A25-2产纤维素酶的酶活力较高,在最适作用pH4.5和最适作用温度55℃下,其羧甲基纤维素酶活力为2.26IU/mL,滤纸酶活力为0.58IU/mL,Avicel酶活力为0.39IU/mL。薄层层析实验表明A25-2具有完整的纤维素酶系统。因此,真菌A25-2可作为饲料加工等生产和纤维素酶相关研究的备选菌株。  相似文献   

吉林省东部半山区“四密25”玉米产量构成因素的浅析     

方向前边少锋  柴寿江孟祥盟谭国波  赵洪祥张丽华杨粉团  李伟堂孙国臣付稀厚  张凤喜 《勤云标准版测试》2006,(7)

四密25是优质高产适应性较强的玉米品种,虽创造出吉林省东部半山区15000kg/hm2的高产记录,但产量波动(7500～15000kg/hm2)较大。为进一步研究其高产稳产的构成因素,1994-2004年,在吉林省东部半山区桦甸市5个乡镇,应用四密25分别进行了品种比较、高产攻关、覆膜种植与裸地种植、不同施肥量、施肥方法、播种方法、播种时期、不同密度及等离子体处理种子的剂量、次数、播种期、化肥利用率的试验。通过对上述各项试验的194份材料统计分析,明确了四密25构成产量模型的3个主导因素为千粒重、穗粒数、公顷穗数。根据回归分析理论,计算出产量与3个主导因素之间的模型,为今后吉林省东部半山区四密25高产稳产提供理论依据。  相似文献   

25%阿米西达悬浮剂与6种药剂混用对辣椒安全性的影响     

肖彤斌  谢圣华  芮凯  吴凤芝  王三勇 《安徽农学通报》2007,13(18):160-161

用新型高效低毒仿生杀菌剂25%阿米西达悬浮剂与生产上常用的6种杀虫剂和杀菌剂设计成7个混用组合,于不同辣椒品种的不同生育期实施叶面喷雾,结果显示,所有混用组合均不会对辣椒的株高和叶片数量造成不良影响,辣椒植株上也未观察到任何药害症状,从而确认了其在辣椒上应用的安全性.  相似文献   

Inhibitory effect of 1,25-(OH)₂D₃ on proliferation of airway smooth muscle cells from passively-sensitized patients     

SONG Ying-fang  LAI Guo-xiang  QI Hao-wen  WU Chang-gui 《园艺学报》2011,27(9):1828-1831

AIM: To investigate the effects of 1,25-dihydroxyvitamin D₃ on the proliferation of passively-sensitized human airway smooth muscle cells (HASMCs), and to explore its potential role in asthmatic airway remodeling.METHODS: HASMCs were passively sensitized with 10% serum from asthmatic patients.1,25-(OH)₂D₃ was used as the interventor.The effect of 1,25-(OH)₂D₃ on the cell proliferation and its optimal concentration were determined by MTT colorimetric assay.The cell cycle analysis was performed by flow cytometry.The expression of proliferating cell nuclear antigen (PCNA) was measured by the method of immunocytochemical staining.RESULTS: 1,25-(OH)₂D₃ at the concentrations of 10^-9-10^-7 mol/L markedly inhibited the cell proliferation and the maximum effect was observed at the concentration of 10^-7 mol/L.This concentration of 1,25-(OH)₂D₃ markedly suppressed the PCNA-positive rate and hampered the G₁/S transition in HASMCs passively-sensitized by asthmatic serum.CONCLUSION: 1,25-(OH)₂D₃ has direct inhibitory effects on the proliferation of passively-sensitized HASMCs in vitro, which may be concerned with the beneficial role of 1,25-(OH)₂D₃ on the prevention and therapy of asthmatic airway remodeling.  相似文献   

海岛棉GbMYB25基因的克隆及表达分析     

陈全家  倪志勇  刘静静  冯文林  刘超  陈章良  康定明  曲延英 《中国农业大学学报》2015,20(1):1-7

根据陆地棉GhMYB25的序列,设计1对引物,通过RT-PCR技术从海岛棉品种新海21号中克隆了1个同源基因,命名为GbMYB25。GbMYB25基因具有1个930bp的开放阅读框,编码309个氨基酸,预测分子量约为34.762ku,等电点为8.08,推测的氨基酸序列中含有2个高度保守的SANT结构域。GbMYB25基因序列包含2个内含子。氨基酸序列比对表明,该蛋白和其他高等植物的MYB蛋白有较高的同源性。进化树分析表明,GbMYB25基因和陆地棉GhMYB25基因处在同一进化树分支。亚细胞定位表明GbMYB25基因在细胞核中表达,实时荧光定量PCR结果表明GbMYB25基因在胚珠(0doa)、纤维(5dpa)和叶片中的表达量较高。以上结果表明,GbMYB25转录因子可能参与棉花纤维发育。  相似文献   

乳牛口服过量维生素A对维生素D3及钙磷代谢的影响   总被引：9，自引：0，他引：9  

金久善  夏兆飞 《畜牧兽医学报》1994,25(1):25-30

从北京郊区某农场选取１０头健康成年黑白花乳牛，随机分为试验（５头，每周每头口服维生素Ａ１００万单位）和对照（５头，不作任何处理）两组。８周以后检测发现，试验组血清钙和无机磷水平呈下降趋势；尾椎骨皮质变薄，密度下降，呈现骨质吸收现象；血清碱性磷酸酶显著下降（Ｐ＜０．０５）；其活性代谢产物２５－羟维生素Ｄ３（２５－ＯＨ－Ｄ３）极显著下降（Ｐ＜０．０１）；１，２５－二羟维生素Ｄ３［１，２５－（ＯＨ）２－  相似文献   

应用25%菊乐合酯乳油防治马尾松毛虫     

汤荣堂  周肃富 《浙江林学院学报》1991,8(3):378-382

1988～1989年,金华县进行应用25％菊乐合酯乳油防治马尾松毛虫药效试验,并在大面积防治中推广应用了这种新的复配农药。结果表明:应用25％菊乐合酯孔油防治马尾松毛虫3～4龄幼虫在地面超低量喷雾防治时,60ml/hm~2就可取得96.3％的效果,用药成本1.05元/hm~2,比用2.5％溴氰菊酯乳油(3.60元/hm~2),20％杀灭菊酯乳油(2.55元/hm~2)分别下降2.55元/hm~2和1.50元/hm~2;飞机超低量喷雾防治马尾松毛虫3～4龄幼虫用药120g/hm~2即可,效果可达94.8％,用药成本比用2.5％溴氰菊酯乳油、20％杀灭菊酯礼油分别下降1.50元/hm~2和0.45元/hm~2。  相似文献