Vitamin D status is seasonally stable in northern European dogs     

Emma A. Hurst  Natalie Z. Homer  Adam G. Gow  Dylan N. Clements  Helen Evans  Donna Gaylor  Susan Campbell  Ian Handel  Richard J. Mellanby 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2020,49(2):279-291

  相似文献   

Effects of calcifediol supplementation on markers of chronic kidney disease-mineral and bone disorder in dogs with chronic kidney disease     

Valerie J. Parker  Adam J. Rudinsky  Jason A. Benedict  Azadeh Beizaei  Dennis J. Chew 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(6):2497-2506

  相似文献   

The effect of intramuscularly administered vitamin D3 on serum vitamin D metabolites and electrolytes in vitamin D3 deficient dairy cows     

M Sturén 《Acta veterinaria Scandinavica》1985,26(2):179-190

The effect of intramuscular administration of vitamin D₃ (1×10⁶ IU D₃/100 kg bodyweight) to 3 different dairy breeds on the serum levels of vitamin D₃, 25-OH-D₂, 25-OH-D₃, Ca, inorganic P and Mg was studied.The vitamin metabolites and the electrolytes were analysed on 9 occasions during a 36-day period. Vitamin D₃ was analysed on 6 occasions during the same period. No significant breed differences were observed except for 25-OH-D₃ (P ≤ 0.05). The D₃ level rose in 1 day from < 2 ng/ml to 906 ng/ml and decreased to below 50 % of the peak level after 6 days. At the end of the experiment (day 36) vitamin D₃ was < 2 ng/ml. 25-OH-D₃ rose from < 2 ng/ml to 106 ng/ml in 6 days and stayed at this level during the whole experiment. 25-OH-D₂ decreased from 16 ng/ml to 5 ng/ml during the observation period.  相似文献   

Study on Relative Bioavailability of 1α-Hydroxycholecalciferol to 25-Hydroxycholecalciferol in Broiler Chickens     

HAN Jin-cheng  WANG Jian-guo  CHEN Guan-hua  ZHANG Jin-long  QU Hong-xia  ZHANG Jin-liang  XI Li  YAN Yong-feng 《中国畜牧兽医》2015,42(12):3246-3252

The study was conducted to determine the relative bioavailability (RBV) of 1α-hydroxycholecalciferol (1α-OH-D₃) to 25-hydroxycholecalciferol (25-OH-D₃) in Ross 308 broiler chickens from 1 to 42 days of age.300 one-day-old Ross 308 broilers were randomly assigned into 6 groups with 5 replicates per group and 10 chicks per replicate.The six groups were fed with basal diets in which 2.5,5.0,10.0 μg/kg 25-OH-D₃ and 1.25,2.5,5.0 μg/kg 1α-OH-D₃ were added,respectively.The RBV of 1α-OH-D₃ to 25-OH-D₃ was determined by the slope ratio method.The results showed that average body weight gain (ABWG),feed to gain ratio (F/G),plasma phosphorus (P) were improved with the 1α-OH-D₃ and 25-OH-D₃ addition and the tibia and femur weight,length,and ash weight were linearly increased with the levels of 1α-OH-D₃ or 25-OH-D₃ increasing (P< 0.05).Using ABWG,F/G,and plasma P as the criteria,RBV of 1α-OH-D₃ to 25-OH-D₃ were 2.16,2.56,and 2.77.Using tibia weight,length,width,and ash weight as the criteria,RBV of 1α-OH-D₃ to 25-OH-D₃ were 1.80,1.88,2.42,and 2.04.Using femur weight,length,and ash weight as the criteria,RBV of 1α-OH-D₃ to 25-OH-D₃ were 1.89,1.98,and 2.18.These results indicated that the activity of 1α-OH-D₃ was approximately 2.17 times higher than that of 25-OH-D₃ in promoting growth performance and bone mineralization in broiler chickens from 1 to 42 days of age.  相似文献   

1,25-dihydroxyvitamin D3 inhibits nuclear factor kappa B signaling pathway in passively sensitized human airway smooth muscle cells     

SONG Ying-fang  LAI Guo-xiang  LIU De-ling  LIN Qing-an  LIAO Yun-hai 《园艺学报》2013,29(11):2044-2048

AIM:To investigate the effects of 1,25-dihydroxyvitamin D3 ［1,25-(OH)2D3］ on nuclear factor kappa B (NF-κB) signaling pathway in passively sensitized human airway smooth muscle cells (HASMCs). METHODS:HASMCs were passively sensitized with 10% serum from asthmatic patients. 1,25-(OH)2D3 was used as an interfering factor. Electrophoretic mobility shift assay (EMSA) was used to detect the DNA-binding activity of NF-κB. Immunocytochemical staining was used to observe the nuclear translocation of NF-κB p65. Western blotting was used for IκBα and phosphorylated IκBα protein detection. Real-time fluorescence quantitative PCR was used to determine vitamin D receptor (VDR), vitamin D 24-hydroxylase (CYP24) and IκBα mRNA expression. The mRNA expression of IκBα in HASMCs after actinomycin D treatment was also determined. RESULTS:(1) 1,25-(OH)2D3 significantly attenuated the DNA-binding activity of NF-κB and the nuclear translocation of NF-κB p65 in HASMCs passively sensitized by asthmatic serum. (2) 1,25-(OH)2D3 enhanced IκBα mRNA stability and inhibited IκBα protein phosphorylation in passively sensitized HASMCs, thus increasing IκBα expression in these HASMCs. (3) 1,25-(OH)2D3 up-regulated VDR mRNA level and evoked its functional response in passively sensitized HASMCs. CONCLUSION: 1,25-(OH)2D3 enhanced the expression of IκBα and therefore inhibited NF-κB signaling passway in HASMCs. This effect may be dependent on VDR, and responsible for the inhibitory effect of 1,25-(OH)2D3 on passively sensitized HASMCs.  相似文献   

新疆红花随体染色体及25S rDNA位点分析     

王力军  严兴初 《中国油料作物学报》2012,34(3):245-248

利用荧光原位杂交技术研究我国新疆红花25S rDNA在染色体上的位点数目,同时分析了其随体染色体数目。研究结果显示：新疆红花有2对随体染色体;4对染色体存在25S rDNA位点,其中3对染色体上的杂交信号较强,可稳定检出,有时还可检测到1对染色体上较弱的杂交信号。与国外红花品种相比,新疆红花rDNA位点数目多,表明新疆红花与国外红花品种间核仁组织区结构存在较大差异。这些结果将为研究红花染色体进化及其准确的核型分析提供一定的参考。  相似文献   

土壤团聚体稳定性评价方法比较   总被引：22，自引：3，他引：19  

苏静  赵世伟 《水土保持通报》2009,29(5):114-117

土壤团聚体稳定性是评价土壤结构和土壤物理形状的重要指标.对土壤团聚体分形维数、土壤团聚体平均重量直径和>0.25 mm团聚体含量3种土壤团聚体结构评价的方法进行了探讨.结果表明,分形维数与>0.25 mm团聚体的含量呈负相关性,但是平均重量直径与>0.25 mm团聚体的含量有正的相关性.这三种方法都能应用来评价土壤结构,但是分形维数不仅表示土壤结构,还能说明土壤水分和土壤其它的物理性质.因此,分形维数是评价土壤结构的较好的方法.  相似文献   

大豆新品种北豆25的选育及栽培技术要点     

刘玲雪  朱化敏  冯涛  吴亚晶  李红林  王平  贺海生 《大豆科技》2009,(5):66-66,68

大豆新品种北豆25是黑龙江省建三江科研所以绥农11为母本,哈94—3164为父本进行有性杂交,系谱选育而成,具有高产、稳产、高油、中抗灰斑病的优良特性。  相似文献   

大豆根围丛枝菌根真菌鉴定   总被引：3，自引：1，他引：2  

蔡柏岩  接伟光 《大豆科学》2009,28(3)

通过对大豆根围丛枝菌根(arbuseular myeorrhiza,AM)真菌鉴定和分子检测,为大豆菌根深入研究奠定基础.采集大豆根系和根围土壤,采用湿筛倾析法分离大豆根同土壤AM真菌孢子.依据AM真菌孢子形态特征及25S rDNA D1/D2区域序列分析对其进行分类鉴定,并应用Nested-PCR技术检测根围土壤AM真菌侵染大豆根系情况.结果表明:大豆根围土壤中存在2种AM真菌:根内球囊霉(Glomus intraradices)、摩西球囊霉(Glomus mosseae),并且两者均侵染大豆根系.  相似文献   

刚地弓形虫GRA25基因的克隆及序列分析     

徐晓佩  刘文阁  张念章  陈佳  周东辉  朱兴全  徐前明 《中国畜牧兽医》2016,43(3):650-655

本研究旨在对来源于不同宿主和地理分布的22株弓形虫的GRA25基因进行PCR扩增并测序,对获得的GRA25基因序列进行比对,利用MP和ML两种方法对不同分离株的GRA25基因构建系统进化树。利用生物信息学软件将弓形虫RH株的GRA25基因翻译成氨基酸序列,对其编码蛋白的生物学特征进行分析。结果显示,弓形虫GRA25基因序列有2种长度,分别为939和948 bp。序列比对结果显示,GRA25基因在不同虫株间有82个核苷酸变异位点,变异率为0~4.4%。进化分析结果显示,用GRA25基因不能区分弓形虫基因Ⅰ型和Ⅱ型虫株。生物信息学分析预测弓形虫GRA25蛋白含有7个亲水区域,10个α-螺旋,3个β-折叠,8个无规则卷曲和8个潜在的线性B淋巴细胞抗原表位。本研究结果表明,GRA25基因不能作为标记分子区分不同基因型的弓形虫虫株,但可能作为疫苗候选分子研制新型抗弓形虫基因疫苗或表位肽疫苗。  相似文献