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Pyrosequencing was used to study the effect of rotation and tillage on total bacterial communities. We designed primers to the bacterial 16s rDNA and amplified DNA from soil samples from a long-term tillage/rotation trial in Kansas for two seasons. The 2 × 2 factorial trial had two rotation treatments (wheat-wheat and wheat-soybean) and two tillage treatments (conventional and no-till). A total of 20,180 16s rDNA sequences were generated and 2337 operational taxonomic units (OTUs) were assembled using a 97% similarity cut-off. The phylum Proteobacteria represented 38% of 299 identified taxa. The second most abundant phylum was Acidobacteria, making up 20% of the sequences, the majority of which were Acidobacteria Group 1. The phyla Actinobacteria and Gemmatimonadetes comprised 12% and 3.5% of the sequences. Other groups detected included TM7, Nitrospira, Verrucomicrobia, and Bacteroidetes. Some clusters of Acidobacteria Group 1 were more frequent in continuous wheat versus wheat-soybean rotation, some Acidobacteria Group 2 were more frequent in no-till, and some Acidobacteria Group 4 were more frequent in wheat-soybean rotation. These results were validated by quantitative real-time PCR. Pyrosequencing provided taxonomic information about the overall bacterial community, and detected community shifts resulting from different cropping practices. 相似文献
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大豆灰斑病菌毒素生物活性分析 总被引:11,自引:1,他引:10
大豆灰斑病菌[Cercosporium sojina(Hara) Liu&Guo]可以产生有毒代谢物。滤液透析及热稳定性测试结果表明:该毒素可以穿过半透膜,为较小分子化合物并具有很强的热稳定性。该菌培养物浸提液经浓缩,硅胶柱层析可得粗毒素。生物测定结果表明:该毒素对大豆幼苗及叶片具有致萎作用。针刺叶片可产生类似真菌感染的病斑。浸渍处理后,可使叶组织失绿、萎蔫最后坏死,同时毒素对抗性不同的品种有鉴别作用。 相似文献
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RAPD标记在大豆品种鉴定中的应用初探 总被引:4,自引:0,他引:4
以十六烷基溴化铵(CTAB)选择性沉淀DNA的方法,提取大豆种子、新鲜叶片或冷冻干燥叶片的总DNA,所得DNA纯度好,降解少,分子量大,完全可以用于RAPD分析。本文就RAPD标记手段在大豆品种鉴定中的应用作一简报,仅OPA-04就能将供试的17份大豆品种区分开,表明RAPD用于种子纯度鉴定及品种鉴定是可行的。 相似文献
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采用盆栽试验、田间试验及室内分析相结合的方法 ,研究了含有沸石、腐殖酸、稀土的大豆专用复混肥对轮作、连作大豆的增产机理。结果表明 ,施用大豆专用复混肥对轮作、连作大豆具有明显的改土、增加肥效、抗逆、提高光合效率和促进大豆生长的作用 ,最终表现出明显的增产效果。大豆专用复混肥的综合施用效果以连作大豆好于轮作大豆 相似文献
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《Plant Production Science》2013,16(4):434-439
AbstractFlooding during germination inhibits the sprouting and emergence of soybean [Glycine max (L.) Merr.], reducing subsequent growth and yield. Previous studies revealed that cultivars tolerant to pre-germination flooding have a mechanism of reducing water absorption speed (WAS) during the initial stage of inundation; however, seed structures involved in WAS have not been fully clarified to date. The objectives of this study were to identify possible seed structures responsible for cultivar difference in WAS and pre-germination flooding tolerance. WAS of two tolerant cultivars (Peking and Williams) and two susceptible cultivars (Nakasennari and Enrei), which were identified in our previous study, was compared in relation to seed structures. In Peking, WAS was markedly lower than that of the other cultivars, either in intact seed or seed with the seed coat removed, suggesting that both the seed coat and the embryo have a mechanism of reducing WAS in this cultivar. WAS of the hilum side tended to be higher than that of the back side, and sealing of the micropyle significantly lowered WAS, showing that the micropyle rather than the hilum appeared to be responsible for the higher WAS of the hilum side regardless of cultivar. A comparison of cross section area of the hilum revealed that the tolerant cultivars tended to have a larger area than the susceptible cultivars, suggesting that an inner space of the hilum can act as a reservoir at the initial stage of inundation, thereby reducing WAS in tolerant cultivars. 相似文献