中国大麦种质资源Wx蛋白分析及糯大麦分子标记验证   总被引：1，自引：0，他引：1  

朱彩梅  张京 《麦类作物学报》2010,30(5):813-819

利用1D-SDS-PAGE分析了中国167份不同直链淀粉含量类型的大麦种质资源Waxy 蛋白类型,发现在低直糯大麦和无直糯大麦两种类型中,可能存在四种生化遗传变异类型。其中有两种与无直糯大麦有关,一种是Wx基因可以正常转录,但转录产生的Wx蛋白无活性,不能催化直链淀粉合成;另一种是Wx基因不能正常转录,既无Wx蛋白产生,也无直链淀粉合成。另外两种与低直糯大麦有关,一种是Wx基因可以正常转录,但转录产生的Wx蛋白活性有限,催化直链淀粉合成的功能降低;另一种是Wx基因不完全正常转录,产生的Wx蛋白数量有限,影响直链淀粉合成。利用4个大麦与小麦的STS标记和2个大麦与小麦的SSR标记对不同Wx蛋白类型进行糯性鉴定,结果表明,迄今利用的Wx基因的STS引物P1可能只适合用于CDC Candle遗传类型相同的糯大麦品种或基因型的鉴定。其它标记均不能用于鉴定大麦糯性胚乳表型,因此当前对于糯大麦育种及Wx分子鉴定研究而言,还有待于开发新的分子标记。  相似文献   

Determination of Amylose Content and Its Relationship with RVA Profile Within Genetically Similar Cultivars of Rice (Oryza sativa L.ssp.japonica)     

WANG Xin-qi  YIN Lin-qing  SHEN Ge-zhi  XU Li  LIU Qiao-quan 《中国农业科学(英文版)》2010,9(8)

Anther culture pure lines with antisense Wx gene were generated by Agrobacterium tumefaciens-mediated co-transformation followed by anther culture in transgenic rice (Oryza sativa L.ssp.japonica).The antisense Wx transgenic pure lines were used to analyze the differences and the relationship between RVA eigenvalues among the lines with different amylose contents.77 pure lines of anther culture having antisense Wx gene were studied for the amylose content of its offspring cultivar,Wuyunjing 7.It was found that levels of amylose content were similar to the control in 19 lines (16.0%);1-5%lower than control in 50 lines,of which,within 11.0-13.9% in 40 lines;and 3.1-4.0% in 8 lines.The effect of the amylose content on RVA eigenvalue was analyzed through the RVA profile graphic comparison and test of significance for RVA eigenvalues.The analysis of RVA profiles with different amylose contents indicated that there were two kinds of RVA profiles within genetically similar cultivars.The lines with 3.1-4.0% of amylose content were distinctly different from other lines and conventional glutinous rice.The comparison of the similarity curve of RVA profile of the lines with different amylose contents showed that the lines with lower amylose content had a distinguished RVA profile with the curve increasing gradually,but not exceeding the first apex.The test of significance for eigenvalues of the RVA profile in the lines with different amylose contents indicated that there were five eigenvalues remarkably different among the lines with 3.1-4.0% of amylose content compared with other two groups.Further,there were three eigenvalues remarkably different among the lines with 11.0-13.5% of amylose content compared with control group.An optimized mathematical model,which characterizes the relationships between the amylose content and RVA eigenvalue,has been established in this study.From this model,it was clearly indicated that the parameters PeT and SBV were more related to amylose content.It was concluded that the differences in amylose content not only affected the eigenvalues of RVA but also caused different RVA profiles within genetically similar cultivars.The introduction of antisense Wx gene could lead to reduce the amylose content and lay theoretical foundation for quality improvement in rice breeding programs.  相似文献   

Amylose synthesis capacity of the three Wx genes of wheat cv. Chinese Spring     

Hideho Miura  Etsuko Araki  Sawako Tarui 《Euphytica》1999,108(2):91-95

The Wx locus controls amylose synthesis in the cereal endosperm. Hexaploid wheat (Triticum aestivum L.) has the three Wx loci on chromosomes 7A ( Wx-A1), 4A (Wx-B1) and 7D (Wx-D1). To verify the effects of null alleles on reducing amylose content and determine the amylose synthesis capacity of each Wx gene independently and accurately, we produced eight possible types of recombinant lines carrying different null alleles at the Wx loci under the ‘Chinese Spring’ genetic background. Amylose content varied from 0% of the waxy ‘Chinese Spring’ to 25% of the ‘Chinese Spring’ normal type. The reducing effect of the single null alleles was the largest in Wx-B1b, and there was no significant difference between Wx-A1b and Wx-D1b. More than 3% reductions in amylose content were detected in the double null types. The results of the double null lines further demonstrated that for the capacity of amylose synthesis, Wx-B1a predominates and produces 21–22% amylose, followed by Wx-D1a (20–21%) and Wx-A1a (15–18%). These significant differences were partly correlated with variation in the amounts of the Wx proteins produced by different Wx genes. However, comparisons of the double null lines with the single null or normal lines indicated that amylose content was not linearly proportional to the number of the Wx genes, suggesting that the Wx genes act in an epistatic manner. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

小麦地方品种Wx基因构成分析   总被引：15，自引：2，他引：13  

王子宁  郭北海  张艳敏  温之雨  李洪杰  石云素 《华北农学报》1999,14(3):5-9

用ＳＤＳ－ＰＡＧＥ电泳技术,从９００份小麦（Ｔ．ａｅｓｔｉｖｕｍ）地方品种鉴定出普通小麦Ｗｘ－７Ｄ基因缺失材料一份,Ｗｘ－４Ａ基因缺失材料六份,含三个基因的材料为８９３份,出现频率为正常类型〉Ｗｘ－４Ａ〉Ｗｘ－７Ｄ〉Ｗｘ－７Ａ,Ｗｘ－７Ｄ缺失然因的发现,再次证明我国小麦资源是一特殊类群,这些材料的发现,为我国开展面各品质育种研究打下了坚实的材料基础,在小麦淀粉遗传控制研究和品质育种方面具有深远的理  相似文献   

江苏淮北部分小麦品种品质性状相关重要基因的检测     

周淼平  杨丹  杨学明  姚金保  张平平  张鹏 《江苏农业学报》2014,(6)

为了解江苏淮北小麦品种重要品质性状相关基因状况,以20份近期审定的徐麦系列和淮麦系列小麦品种以及12份小麦对照材料为试验材料,采用SDS-PAGE电泳与PCR检测相结合,检测了小麦高分子量麦谷蛋白亚基;同时采用共显性PCR标记筛查1BL/1RS易位系以及Wx基因突变型。结果显示,江苏淮北小麦品种Glu-A1位点含3种亚基,其中1亚基占75%;Glu-B1位点有4种亚基,7+8亚基占60%;Glu-D1位点有3种亚基,5+10亚基占50%;1BL/1RS易位系有14份,占全部品种的70%;所有品种均为Wx基因野生型。  相似文献